BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,501	G→A	5.6%	A17A (GCC→GCT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,501	0	G	A	5.6%	390.2 / 9.7	178	A17A (GCC→GCT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (77/89); new base A (5/6); total (82/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 181 total aligned reads displayed.

TCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTA  >  CP000730/36362‑36639

 cttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTACATCTTAGATAGTAGCCAACGGc                                                                                                                                          >  1:460684/1‑141 (MQ=25)

    ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAAATAGTAGCCAACggatgc                                                                                                                                       >  2:478196/1‑137 (MQ=25)

     cAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACa                                                                                                                                      >  2:519093/1‑141 (MQ=35)

         tttGAGACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGa                                                                                                                                  <  2:221470/141‑1 (MQ=25)

          ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAt                                                                                                                                 <  1:265276/141‑1 (MQ=35)

           tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCATAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                                                                                                                >  1:465783/1‑141 (MQ=25)

             aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAc                                                                                                                              <  2:502788/141‑1 (MQ=35)

                      tGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTGACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                                                                                                                     <  1:123703/141‑1 (MQ=17)

                          gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                                                 <  1:207029/141‑1 (MQ=32)

                                tCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTta                                                                                                           <  1:547771/141‑1 (MQ=32)

                                    gagcccAGCGGTAGCTCGTGGAGTGTGTAACGTTTACACTACGTTTTCTTAGTATGCTCCATATACCACGATAACTCAAGTTTTATCTTATATAGGAGCCAACAGCTACAGTGATAACATCCTTGTGAAATTGttggttgt                                                                                                       <  2:276829/138‑7 (MQ=2)

                                     aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                                                      <  1:519093/141‑1 (MQ=32)

                                       cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                                                                                    <  1:37194/141‑1 (MQ=32)

                                             ggTAGAGCGTTGAATGATGAACGTTTACAGCCCGTGCCCTAAATATTTCAGATATATCACGATAACACAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTCGTTAAATTGTTTATATCTGAAATAGt                                                                                              >  1:489431/1‑141 (MQ=11)

                                              gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                                             <  1:481845/141‑1 (MQ=32)

                                                   ccGTTGAATGATGAACGTTTACTCCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAc                                                                                        <  1:167627/141‑1 (MQ=17)

                                                     gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTCTTTATATCTCAAATAGTTCATACag                                                                                      <  1:334069/141‑1 (MQ=17)

                                                               gaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATGCTTGTTAAATTGTTTAGATTTGAATTATTTAACAAAGATAACTCCtt                                                                            >  2:520407/1‑141 (MQ=11)

                                                                     ttACACCACGTTCCCTTAATATTTCCGCTATATTACGATCACTCAATGTAGATCTTCGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACGGAAGACTCCTTTTTGtt                                                                      <  1:137284/141‑1 (MQ=11)

                                                                         accacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaaaa                                                                  >  2:530222/1‑141 (MQ=32)

                                                                                    ttAATATTTCAGATATATCACGCTAACTCAATGTATAACCTAGATAGTAGCCAACGGCTACAGTGTTAACATCCTTGTCAAATTGTTTATATCTAAAATAGTTCATACAGAAGTGTCGTTTTTGTTAAAATAGTACTATaa                                                       >  2:48732/1‑141 (MQ=11)

                                                                                       atctttCAGCCATATCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTCATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAAtt                                                    <  1:554685/138‑1 (MQ=1)

                                                                                          ttacAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCaa                                                 <  1:478306/138‑1 (MQ=25)

                                                                                               gATATATCACGATAGCTAAGTGTAGATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTg                                            >  1:490584/1‑141 (MQ=11)

                                                                                                   tagcACGATAACTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc                                        <  2:183814/138‑1 (MQ=2)

                                                                                                     tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg                                      >  1:288255/1‑141 (MQ=35)

                                                                                                       aCGATAACTCAATGTATATCTTAGATAGTAGACAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTTTTTTTTTCTATAAATTCGACTTTTGATCAGaa                                    >  2:140991/1‑141 (MQ=11)

                                                                                                         gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcc                                  >  2:550425/1‑141 (MQ=34)

                                                                                                           tagcccAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                <  1:227555/136‑1 (MQ=255)

                                                                                                           tagcTCAATGCATATCTTAGATAGTAGCCAATGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                <  2:57855/138‑1 (MQ=255)

                                                                                                           tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                >  1:163003/4‑141 (MQ=9)

                                                                                                           tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                >  1:350448/4‑141 (MQ=9)

                                                                                                           tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                <  2:135747/138‑1 (MQ=9)

                                                                                                           tagcTCAATGCATATCTAAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATCGTTTATATCTGAAATAGTTCCTACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                <  2:447164/138‑1 (MQ=11)

                                                                                                           tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                                <  1:638325/141‑1 (MQ=35)

                                                                                                           tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTCTGCAACAGAACCGt                                <  1:356719/141‑1 (MQ=37)

                                                                                                           aagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                <  1:421961/138‑1 (MQ=12)

                                                                                                            agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                               >  2:243286/3‑141 (MQ=12)

                                                                                                            agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                               >  1:581353/3‑141 (MQ=12)

                                                                                                             gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                              >  2:442650/2‑141 (MQ=12)

                                                                                                             gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                              >  2:350177/2‑141 (MQ=12)

                                                                                                             gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                              >  1:607485/2‑141 (MQ=12)

                                                                                                             aCTCAATGTATATCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                              <  2:42401/141‑1 (MQ=38)

                                                                                                              cacAATGCATATCTTAAATAGTCGCCAACGGCTACAGTGATAACACCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAAAGAACCGtatt                             >  2:584778/3‑141 (MQ=11)

                                                                                                              cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                             >  2:620215/1‑141 (MQ=16)

                                                                                                              atCAATGTATATGTTAGATGGCAGCCAACGGCGACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatt                             <  1:24713/140‑1 (MQ=255)

                                                                                                               tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                            >  1:77760/1‑140 (MQ=16)

                                                                                                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                           <  1:199074/141‑3 (MQ=255)

                                                                                                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                           <  2:247758/141‑3 (MQ=255)

                                                                                                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                           >  1:332808/1‑139 (MQ=16)

                                                                                                                cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                           <  2:171153/141‑3 (MQ=255)

                                                                                                                cAATGCATATCTTAGATAGTACAAAACGTCTGCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                           >  1:659988/1‑139 (MQ=11)

                                                                                                                 aaggcatatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttac                          <  2:156670/136‑9 (MQ=11)

                                                                                                                  aTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                         <  1:136447/141‑5 (MQ=255)

                                                                                                                   ttattatCATAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATAACTTAAATAGTTCATACAGAAGACCCCTTTTTGTTAAAATTATATTATAACTTCAACTTTGCAACAGAAcctcctaactgt                        <  2:33658/137‑11 (MQ=11)

                                                                                                                    gctgagtttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                       <  2:574644/134‑7 (MQ=11)

                                                                                                                    gcatatCTTAGATAGTAGGCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactat                       <  1:304558/139‑12 (MQ=1)

                                                                                                                    gcatatCTTAGATAGTAGCCAATGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                       >  1:156161/3‑135 (MQ=11)

                                                                                                                    gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                       <  1:360759/139‑7 (MQ=255)

                                                                                                                     catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                      <  1:658453/140‑13 (MQ=11)

                                                                                                                      atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                     <  2:165729/141‑1 (MQ=255)

                                                                                                                      atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                     >  2:375297/1‑128 (MQ=11)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTATAATTTTATTATAAATTCAACTTTGCAACAGAACCGtattttttgagta                    >  2:6183/1‑132 (MQ=11)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    >  2:617003/1‑127 (MQ=11)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    >  1:428510/1‑127 (MQ=11)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    <  1:276482/141‑15 (MQ=11)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    >  1:73224/1‑127 (MQ=11)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                    >  2:274987/1‑132 (MQ=255)

                                                                                                                       tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                    <  1:619866/141‑10 (MQ=255)

                                                                                                                       tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                    >  1:193156/1‑127 (MQ=11)

                                                                                                                       tatCTTAGAAAATAGCCAACGGCAACAGTGATAAAATACTTGTTAAATTGTTTATATCCGAAATTGTTAATACAGATAACTCCACTTTGTTAAAATTAATTAATAAATTCAACTTTGCACCAGAACCGtatttaatgagta                    >  2:260142/1‑132 (MQ=2)

                                                                                                                       tatCTTAAATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    <  1:364467/141‑15 (MQ=11)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAACTTCAACTTTGCAACAGAACCGtattttatgagtac                   >  1:323479/1‑131 (MQ=11)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtcttttatgagtac                   >  1:153009/1‑131 (MQ=11)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   <  2:275155/141‑11 (MQ=255)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   >  2:312733/1‑131 (MQ=255)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   <  1:596166/141‑11 (MQ=255)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   <  2:120678/141‑11 (MQ=255)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   <  2:124434/141‑11 (MQ=255)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGATTaga                   <  2:460684/141‑1 (MQ=255)

                                                                                                                        atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                   >  1:459673/1‑141 (MQ=255)

                                                                                                                         tCTTAGTTAGAAGCCAACGGCTAAAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACGGTGCAACAGAACCGtattttatgagtaca                  <  1:25823/141‑12 (MQ=11)

                                                                                                                          tttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAgaga                 <  2:197381/140‑1 (MQ=255)

                                                                                                                          cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacac                 >  1:275819/1‑129 (MQ=11)

                                                                                                                          cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacac                 <  2:657165/141‑13 (MQ=11)

                                                                                                                          cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacac                 <  2:614419/141‑13 (MQ=11)

                                                                                                                           ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTCTATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                <  1:125907/141‑14 (MQ=11)

                                                                                                                           ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                <  1:97107/141‑14 (MQ=11)

                                                                                                                           ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                >  2:34174/1‑141 (MQ=255)

                                                                                                                           ttAGATAGGAGCCAACGGCTAAAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                <  2:652933/141‑1 (MQ=255)

                                                                                                                            tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTATTTTATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacacac               >  2:340468/1‑127 (MQ=11)

                                                                                                                            tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttattagtacactc               >  2:60019/1‑127 (MQ=11)

                                                                                                                            tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc               <  2:632552/141‑15 (MQ=11)

                                                                                                                             ataTAGTAGCCTACGGCTACAGTGATAACATCCTTGTTAAAAAGTTTGTAGGTGAAATAGTTCATACAGGGGGCTGCTGTTTGTTAAAATTATCCTATAAACTCAACTTTGCAAATGAACCGTATGATGGAATAGAGATgg              <  2:265609/139‑2 (MQ=14)

                                                                                                                                tagtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgt           >  2:665581/1‑140 (MQ=17)

                                                                                                                                  ttagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTa         <  2:20982/140‑1 (MQ=255)

                                                                                                                                    agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAAc       >  1:596769/1‑141 (MQ=255)

                                                                                                                                     gCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATCTTAAAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACa      >  1:348072/1‑141 (MQ=255)

                                                                                                                                      ccAACGGCTACAGTGATAACATCCTTGGTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTTCAACGGAACCGTATTATGGAATAGAGATGTTGGTagcct     >  2:23509/1‑137 (MQ=255)

                                                                                                                                         aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta  <  1:34174/141‑1 (MQ=255)

TCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTA  >  CP000730/36362‑36639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`