Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,378 C→T 100% D586N (GAT→AAT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,3780CT100.0% 173.1 / NA 53D586N (GAT→AAT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATT  >  CP000730/39241‑39513
                                                                                                                                         |                                                                                                                                        
aaTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCCTAAGATCTATAAATAttttt                                                                                                                                       >  2:529822/1‑140 (MQ=255)
aaTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGAGCTATAAATAttttt                                                                                                                                       >  1:83204/1‑140 (MQ=255)
 aTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtattct                                                                                                                                      <  2:8607/141‑1 (MQ=255)
      catcattatGTTTGGATTATCTTTATCTTATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTAtg                                                                                                                                 <  2:48410/141‑1 (MQ=255)
        tcatcatGTTTGGATTATCTTTATCATACGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTAGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTAtgtg                                                                                                                              <  1:377719/141‑1 (MQ=255)
            catGTTTGGATTCTCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCGTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAAATAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGtttt                                                                                                                          <  1:395986/141‑1 (MQ=255)
              ttttttGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTtttat                                                                                                                        <  2:46771/139‑1 (MQ=255)
                              ttCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCAt                                                                                                        <  1:54210/140‑1 (MQ=255)
                              aTCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCAt                                                                                                        <  1:54047/141‑1 (MQ=255)
                               tCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTCTAAATATTTTCTTTATGTGTTTTATTTACGACTTGTGGCATa                                                                                                       >  1:203931/1‑141 (MQ=255)
                                   atGATATAAACCACCCCATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATACGATCTATAAATATTTTCTTATGCTGTTTTTTTTACGACCTGTTGCATACGAt                                                                                                   >  2:442653/1‑141 (MQ=255)
                                     gATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATcg                                                                                                 >  2:174444/1‑140 (MQ=255)
                                     gATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCa                                                                                                 >  2:553834/1‑141 (MQ=255)
                                      atatAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAg                                                                                                <  2:638776/141‑1 (MQ=255)
                                       tataAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGt                                                                                               <  2:421977/141‑1 (MQ=255)
                                        ttaAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGtt                                                                                              <  1:132505/140‑1 (MQ=255)
                                         taACCCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATCAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTa                                                                                             <  2:500019/141‑1 (MQ=255)
                                              caccCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGa                                                                                        >  1:423081/1‑141 (MQ=255)
                                              caccCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGa                                                                                        >  1:423119/1‑141 (MQ=255)
                                                   aaTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGCTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGat                                                                                   <  2:344429/141‑1 (MQ=255)
                                                      ttGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtt                                                                                >  2:2810/1‑141 (MQ=255)
                                                       tGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAATTTTGCATATGATCTATAAATATTTTCTTTGTGTGCTTTGTTCCCTTCTTGTTGCATACCTTCAGTTAATGTATTGATAttt                                                                               >  1:514082/1‑141 (MQ=255)
                                                        gTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtttt                                                                              >  2:90156/1‑141 (MQ=255)
                                                              cAGTTTCTGCTTGTTTCATTTTGATTTCTGCAGTACCGTATTTTCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTgg                                                                        <  2:374719/141‑1 (MQ=255)
                                                                   tctcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaata                                                                   <  1:90156/141‑1 (MQ=255)
                                                                               aTTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTCTGGAAATAATATTTttcttc                                                       >  1:195763/1‑141 (MQ=255)
                                                                                 tttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGAATACCATCAGTTAATAGATTGAGATTTTCTTTGGAAATAATATTTttcttcaa                                                     >  2:603307/1‑139 (MQ=255)
                                                                                 tttGAGTTCTGCAGTACCGGACTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCa                                                     >  2:240163/1‑141 (MQ=255)
                                                                                     ggTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACt                                                 <  1:295252/140‑1 (MQ=255)
                                                                                             ggTACCGGTTTTGCCAATTAAGTTTGCATAGGTTCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCTTCAGTTAATAGTTTGTTATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGttttt                                         <  1:111845/140‑1 (MQ=255)
                                                                                              gTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTc                                        <  2:652087/141‑1 (MQ=255)
                                                                                              gTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTc                                        <  2:188135/141‑1 (MQ=255)
                                                                                                aCCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCgt                                      >  2:631345/1‑141 (MQ=255)
                                                                                                  cGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCgtgt                                    >  1:16567/1‑141 (MQ=255)
                                                                                                   ggATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCgtgtt                                   >  1:278805/1‑140 (MQ=255)
                                                                                                   ggATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTc                                   <  1:111343/141‑1 (MQ=255)
                                                                                                     aTTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTAAATTACGTGTTTTTCATATAACTAGATAAATAACCTATATATCTAGATTGCTATTTTCATTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCtt                                 >  2:657729/1‑141 (MQ=255)
                                                                                                       ttGCAAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTGACGACTTGTTGCATACCATCAGTTGATAGCATGATATTTTTTTTGGAAATAATATTTTTATTTCAAACTTTGTTTTTCTTGTCtttt                               <  2:325758/141‑1 (MQ=255)
                                                                                                           cAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaata                           >  2:631939/1‑141 (MQ=255)
                                                                                                           cAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaata                           >  2:234618/1‑141 (MQ=255)
                                                                                                              ttATGTTTTCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCTTCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGt                        <  2:482490/141‑1 (MQ=255)
                                                                                                                aaGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGa                      <  1:617284/141‑1 (MQ=255)
                                                                                                                    ttGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTATGTTTTTCGTGTCTTTTAATAAGTGAGGTg                  >  2:497153/1‑141 (MQ=255)
                                                                                                                     tGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCTTGTCTTTTAATAAGTGAGGTGc                 >  1:588117/1‑141 (MQ=255)
                                                                                                                     tGCAAAAGAGCTATAAATATTTTCTTTATGTGTTTTATCTACGACTTGTTGCAGACCATCCGTTAATAGAGTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGc                 <  1:317075/141‑1 (MQ=255)
                                                                                                                      ggaTAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATATCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCg                <  2:263114/139‑1 (MQ=255)
                                                                                                                      gCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCg                >  2:114052/1‑141 (MQ=255)
                                                                                                                       cATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTAAGAATTGTTGAATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTACCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGt               <  1:14492/141‑1 (MQ=255)
                                                                                                                         tAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTGATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATACGTAAGGTGCGTTa             >  1:30060/1‑141 (MQ=255)
                                                                                                                         tAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTa             >  1:225472/1‑141 (MQ=255)
                                                                                                                           aGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCCAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAat           >  1:124448/1‑141 (MQ=255)
                                                                                                                              tCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCAAAAATTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATAtt        >  1:57781/1‑141 (MQ=255)
                                                                                                                               cTATAAATATTTTCTTTATGTGTTTTATTTACGGCTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTg       >  1:134617/1‑141 (MQ=255)
                                                                                                                                 ataAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGcc     <  1:24638/141‑1 (MQ=255)
                                                                                                                                    aaTATTTTCTTTATGTGTTTTATTTACTACTTGTTGCATTCTATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGACTTTTAATAAGTGAGGTGCGTTAatatcatatga  <  2:203931/141‑8 (MQ=255)
                                                                                                                                    aaTATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCatt  >  2:538253/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                        
AATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATT  >  CP000730/39241‑39513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: