Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,850 C→T 49.4% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8500CT49.4% 71.0 / 123.3 110intergenic (+81/+27)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base T (15/39);  total (43/67)
Fisher's exact test for biased strand distribution p-value = 2.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAG  >  CP000730/35711‑35986
                                                                                                                                           |                                                                                                                                        
tcATTTAAAAAGTATTTAATGTATAAGCTATTCAGTATAAATTTTTTGGCGAAATATGATTACTTGAACAAGATCTAAAATAAAAGTGTGATCTAAGAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAcc                                                                                                                                         <  1:219702/141‑1 (MQ=38)
               ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                                                          >  1:576591/1‑141 (MQ=255)
                tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTc                                                                                                                         <  1:139311/141‑1 (MQ=255)
                 aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAAAAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                                                        >  1:553800/1‑141 (MQ=255)
                         aGCTAATAAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                <  2:562515/141‑1 (MQ=255)
                          gCTATTAAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCt                                                                                                               <  2:383991/141‑1 (MQ=255)
                               tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                                                          <  1:175675/141‑1 (MQ=255)
                                 aGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAATAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTg                                                                                                        >  1:162583/1‑141 (MQ=255)
                                  gTATAAAGGTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAATTAGAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                       <  1:129493/141‑1 (MQ=255)
                                    ataAAGTTTTTGGCGAAAAATGATTACTTGGACAAGAGCTAAAATAAACTTGTGATCTAATAGAAATATAAGGTTCTATTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTTAGTGTTCGCTTAACTTGCTa                                                                                                     <  2:1971/141‑1 (MQ=25)
                                    aaaaacttataggCGAAAAATGATTACTTCAACAAGAGCTAAAATAAAAATTTTATCAAATAAATATATAATGTATTTTTAAAAAATAAAAAAATATAGCTAACCACTAATTAATCATGTCAGTGTTCGCTTAACTTGCTa                                                                                                     <  2:359211/130‑1 (MQ=12)
                                          tttttGGCGAAAAATGATTACTTGAACAAGGGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGcctca                                                                                               >  2:564176/1‑137 (MQ=255)
                                           ttttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgat                                                                                              <  1:358663/141‑1 (MQ=255)
                                           ttttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgat                                                                                              <  1:214941/141‑1 (MQ=255)
                                            tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAACTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                             <  1:539352/141‑1 (MQ=255)
                                            tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                             >  2:192729/1‑141 (MQ=255)
                                             ttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGc                                                                                            <  1:139033/141‑1 (MQ=255)
                                               ggCGAAAAATGATTACTTGAACAAGAGCTAAAATACAATTGTGAACTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGATCACTAACAATTGCTCATGTAAGTATACGCTTAGCTTGCTAGAGTGATGCTa                                                                                          >  2:398352/1‑141 (MQ=21)
                                                 cGAAAAATGATTACTTGAACAAGAGCTAAAGTAAAATTGTAATCTAATAAACATAGAAGGTTATGTTGCAAAGAAAAAAAATATAGCAAACGACTGATTTATCATTAGAGTGTTCGCTTAACTTGCTATCATAGTACTAAt                                                                                        >  2:176185/1‑141 (MQ=21)
                                                    aaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGGTACTAATTTc                                                                                     >  1:107462/1‑141 (MQ=255)
                                                    aaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATACTAATTTc                                                                                     >  1:139794/1‑141 (MQ=255)
                                                     aaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTTGGGCGTCCTGCTAGCATGATGCTAATTTCg                                                                                    <  1:159747/141‑1 (MQ=21)
                                                      aaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTATGTTGCAAAGTAAAAAAATATAGATAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGATAGGATCATGTTAATTTCGt                                                                                   >  2:385892/1‑141 (MQ=21)
                                                             aCTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATATATCATGTCTATGACCACGGAGCTTGCTAGCATGATGCTAATTTCGTGGCATgg                                                                            <  1:657845/141‑1 (MQ=21)
                                                              cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                                           >  2:583744/1‑141 (MQ=255)
                                                                  aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                                                       <  2:162033/141‑1 (MQ=255)
                                                                  aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTACCTTGGCATGGTTCGATCAACGGTGTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                                                       <  1:157358/141‑1 (MQ=18)
                                                                  aaCAAGAGCTAAAATAAAATCGTGATCTAATAAAAATAGAAGGTTAAGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAGTTTCGTGGCATGGAGaaa                                                                       >  2:508716/1‑141 (MQ=18)
                                                                      agagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                                   >  1:667232/1‑141 (MQ=255)
                                                                        agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                 <  1:192729/141‑1 (MQ=255)
                                                                          cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTAAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCCAAAAACCTTac                                                               <  2:576591/141‑2 (MQ=255)
                                                                           taaaataaaaTTGTGATCTAATAAAAATAGGAGGTTCTGTTGCAAAGTCAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGGGCAATTGAGTTGCTAGCATGATGCTAACTTCGTGGCAAGGGGAAAATTCGTAGa                                                              >  1:453624/1‑141 (MQ=17)
                                                                           taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                              >  1:537439/1‑141 (MQ=255)
                                                                               acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                        >  2:147566/15‑141 (MQ=11)
                                                                                caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTACTCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                       <  1:168558/128‑1 (MQ=11)
                                                                                caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAGATCCGTAGATCTGAag                                                       <  1:396503/128‑1 (MQ=11)
                                                                                caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                       <  1:350364/128‑1 (MQ=11)
                                                                                caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                       <  1:289535/128‑1 (MQ=11)
                                                                                caaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                       <  1:198259/128‑1 (MQ=11)
                                                                                 aaaagagtattttaataaAAATGATTGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                      <  1:559834/129‑1 (MQ=11)
                                                                                 aaaagagtattttaataaAAATGATGGTTCTGTTGTTAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                      <  1:513680/129‑1 (MQ=11)
                                                                                  caatTGTGATCTACTAAAAGTAAAAGGTCCTGGTGCAAATTAGAAAAATATAGCTGTACACTAATTTATGATGTCAGTGTTCGGTAAACTTGCTAGTAAGATTCTAATTTCGTGGCATGGCGAAAATCCGTGGTTCTGaga                                                       >  2:322313/2‑139 (MQ=9)
                                                                                  aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                     >  1:104310/12‑141 (MQ=11)
                                                                                  aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                     >  1:641607/12‑141 (MQ=11)
                                                                                  aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGAGGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                     >  2:536122/12‑141 (MQ=11)
                                                                                   aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                    <  2:55909/131‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATTGCTAATCACTAATTTATCATGTCAGTTTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                   <  2:296497/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                   >  1:603793/10‑141 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                   <  1:634279/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                   <  1:658730/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                   <  1:458466/132‑1 (MQ=11)
                                                                                     aaagaatttaataaAAATGATGATTCTGTGTCAAAGAAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                  <  2:106640/133‑1 (MQ=11)
                                                                                      agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                 <  1:611719/134‑1 (MQ=11)
                                                                                      agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                 <  2:200808/134‑1 (MQ=11)
                                                                                       gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                <  2:107916/135‑1 (MQ=11)
                                                                                       gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                <  1:437345/135‑1 (MQ=11)
                                                                                        tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                               <  2:280475/136‑1 (MQ=11)
                                                                                        tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                               <  1:541181/136‑1 (MQ=11)
                                                                                        tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                               <  1:663003/136‑1 (MQ=11)
                                                                                         gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                >  1:659221/1‑141 (MQ=255)
                                                                                         gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                >  1:554976/1‑141 (MQ=255)
                                                                                         ccgtcatattaaaTAGCTGGTTCTGTTCCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCtac                                               <  1:527764/131‑3 (MQ=11)
                                                                                         atttttataaAAATGATGGTTCTGTTGCGAAGTAAAAAATATAGCTAATCTCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                              <  2:27776/135‑1 (MQ=11)
                                                                                         attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                              >  2:442275/5‑141 (MQ=11)
                                                                                         attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                              >  2:331131/5‑141 (MQ=11)
                                                                                         attttaataaAAAGGATGGTTCTGTTGCAAAGTCAAAAATAAAGATAATTAACTGTTTAGCATGTCAGTGTTCGTTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                              <  1:288862/137‑1 (MQ=2)
                                                                                         atttgaagaaaaaTGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                              <  2:454307/133‑1 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACATGCgg                                             >  2:275677/4‑141 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                             <  1:438781/138‑1 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCgg                                             >  1:638948/4‑141 (MQ=11)
                                                                                          agtcatattaaaTGGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCACGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCtacg                                              <  2:436980/132‑4 (MQ=11)
                                                                                          aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                               <  1:531005/141‑1 (MQ=255)
                                                                                           tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCGTGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                            <  2:555063/139‑1 (MQ=11)
                                                                                           tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGAGCTGAAGAGACCTGCGGt                                            <  2:144755/139‑1 (MQ=11)
                                                                                           tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                            <  1:106057/139‑1 (MQ=11)
                                                                                           tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                            >  2:394143/3‑141 (MQ=11)
                                                                                           tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                            <  2:171271/139‑1 (MQ=11)
                                                                                           tttaataaAAATGATGGTTCCTTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                            <  2:362816/139‑1 (MQ=11)
                                                                                           tttaagaaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                            <  2:374664/139‑1 (MQ=11)
                                                                                           tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                              <  1:512420/141‑1 (MQ=255)
                                                                                            ttaataaAAATGATTGTTCTGGTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTACCTTGCCAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                           <  2:60192/140‑1 (MQ=11)
                                                                                            cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                             >  2:515911/1‑141 (MQ=40)
                                                                                             taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                          >  2:290786/1‑141 (MQ=11)
                                                                                              aataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAATAATTTATCATGTCAGTATTCGCTTAACTTGCTAGCATGAAGCTAATTTCGTGGCACGGCACACATCCGTAGACCTGAAGTGAGCcgcgcgc                                           >  2:348392/1‑138 (MQ=14)
                                                                                              aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                         >  2:269111/1‑141 (MQ=11)
                                                                                              aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                         >  2:29345/1‑141 (MQ=11)
                                                                                               ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                        >  1:567860/1‑141 (MQ=11)
                                                                                                  aaaaaggatgggtCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                      <  2:162158/129‑1 (MQ=1)
                                                                                                   aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                      >  1:537689/1‑141 (MQ=35)
                                                                                                   aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                      >  2:598979/1‑141 (MQ=35)
                                                                                                   aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                      >  2:55809/1‑141 (MQ=35)
                                                                                                    aaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCGCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt                                     <  2:357565/141‑1 (MQ=21)
                                                                                                      tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                   <  1:378710/141‑1 (MQ=32)
                                                                                                       aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                  >  1:567056/1‑141 (MQ=32)
                                                                                                       aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                  >  1:1103/1‑141 (MQ=32)
                                                                                                         aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                <  1:125442/141‑1 (MQ=11)
                                                                                                         aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGCCGAAAATCCGTAGATCTGAAGAGACCTGCGTTTCtttttcttta                                >  1:231785/1‑136 (MQ=2)
                                                                                                              tcagTTCCAAACTAAAAAAACAGCGCTTGCCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCg                           <  2:520221/138‑1 (MQ=1)
                                                                                                                                  aatcgCTAACCACTAGTTTATCAGGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCttt       <  2:437230/137‑1 (MQ=1)
                                                                                                                                       ctcaCCTCTAACATATCATGTCAGTGTTCGTTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAg  <  2:609990/138‑1 (MQ=1)
                                                                                                                                           |                                                                                                                                        
TCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAG  >  CP000730/35711‑35986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: