Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	536,758	C→T	91.7%	P202L (CCA→CTA)	rplY →	ribosomal protein L25

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	536,758	0	C	T	91.7%	167.2 / 4.0	68	P202L (CCA→CTA)	rplY	ribosomal protein L25
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base T (21/39);  total (25/43)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

TTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATT  >  CP000730/536620‑536894                                                                                                                                           |                                                                                                                                         ttGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACGGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACGAg                                                                                                                                        >  2:198264/1‑141 (MQ=255)  tGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGa                                                                                                                                       <  1:641283/141‑1 (MQ=255)  tGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGa                                                                                                                                       <  1:570161/141‑1 (MQ=255)  tGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGACGAACAACAAACTGAAGAACTAGa                                                                                                                                       <  1:485506/141‑1 (MQ=255)   gCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGaa                                                                                                                                      <  2:567901/141‑1 (MQ=255)   gCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGaa                                                                                                                                      >  1:207701/1‑141 (MQ=255)   gCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCGATGGAAGGCGAACAACAAACTGAAGAACCAGaa                                                                                                                                      >  2:74493/1‑141 (MQ=255)   gCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCGGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGaa                                                                                                                                      <  1:273329/141‑1 (MQ=255)        tGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTACTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAgttgt                                                                                                                                 >  2:624880/1‑141 (MQ=255)            aaaGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGc                                                                                                                             <  2:339363/141‑1 (MQ=255)             aaGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCg                                                                                                                            <  1:309202/141‑1 (MQ=255)                tAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGaaa                                                                                                                         >  2:560914/1‑141 (MQ=255)                  aCTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGc                                                                                                                       <  2:484024/141‑1 (MQ=255)                     ggCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCaaa                                                                                                                    >  1:368994/1‑141 (MQ=255)                                    gAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaaa                                                                                                     >  1:9301/1‑141 (MQ=255)                                    gAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCCGATTACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGaaaaa                                                                                                     >  1:453634/1‑141 (MQ=255)                                        aCGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTg                                                                                                 <  1:653044/141‑1 (MQ=255)                                        aCGATTCAGCTGAATCAGTAGCAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTg                                                                                                 <  1:14810/141‑1 (MQ=255)                                         cGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGa                                                                                                >  1:505016/1‑141 (MQ=255)                                             tCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAagag                                                                                            <  1:459467/141‑1 (MQ=255)                                             tCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCCATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAagag                                                                                            <  2:202867/141‑1 (MQ=255)                                              cAGCTGAATCAGTAGGAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGt                                                                                           >  1:334814/1‑141 (MQ=255)                                                gCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTaa                                                                                         >  1:60126/1‑141 (MQ=255)                                                 cTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAAt                                                                                        <  1:51781/141‑1 (MQ=255)                                                  tGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAAtt                                                                                       >  2:575320/1‑141 (MQ=255)                                                    aaacaaaagaaaCAGAAATTGCTGCAACTGAAGAACCAACTGAAGAAGAGAACGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAAAAAAAAAAAAAAAAACTGAAGAGTAAtttt                                                                                     <  2:205965/131‑1 (MQ=255)                                                     aTCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAGCTGAAGAGTAATTTTa                                                                                    <  1:331704/141‑1 (MQ=255)                                                           agtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGt                                                                              <  1:412287/141‑1 (MQ=255)                                                           agtaACAGTAGTTGCTCCAACTGAAGAAACAACAGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTAAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGt                                                                              <  2:43397/141‑1 (MQ=255)                                                            gtaACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGtt                                                                             >  1:42899/1‑141 (MQ=255)                                                              aaCAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTAc                                                                           >  2:180270/1‑141 (MQ=255)                                                                  aaagtAGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGACCAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAAAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTa                                                                       <  2:8267/139‑1 (MQ=255)                                                                    aggtGCTCTAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGATTAATTTTAATCTGTTACATTaaa                                                                     <  2:573997/138‑1 (MQ=255)                                                                     gtTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAg                                                                    >  1:226149/1‑141 (MQ=255)                                                                        gCTCCAACTGAAGAACGAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                 <  2:282361/141‑1 (MQ=255)                                                                        gCTCCAACTGAAGAACCAACTGAAGAAGAAAACGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGttt                                                                 <  1:112519/141‑1 (MQ=255)                                                                         cTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGtttt                                                                >  1:429238/1‑141 (MQ=255)                                                                              aatataaaaccaactgaagaaGAAAGCGAAGCTATGGAAGGCCAACAACAAACAGAAGAACTAGAAGTTGTTGGCGAAAGCAAAAAAAAAAAAAAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATAc                                                           <  2:477019/134‑1 (MQ=255)                                                                                   agaaccaactgaagaaGAAATCGTAGCTATGGAAGGCGAACAACAAACTGAAGAACTACAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTACACTTTGt                                                      <  1:418992/141‑1 (MQ=255)                                                                                   agaaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGt                                                      >  1:601687/1‑141 (MQ=255)                                                                                     aaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAGGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTGTAATTTGttt                                                    >  2:264540/1‑141 (MQ=255)                                                                                     aaccaactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGttt                                                    >  2:502045/1‑141 (MQ=255)                                                                                         agctgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACGGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACa                                                <  2:282186/139‑1 (MQ=255)                                                                                         aactgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACa                                                <  1:337322/141‑1 (MQ=255)                                                                                          actgaagaaGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACaa                                               <  1:120036/141‑1 (MQ=255)                                                                                            tgaagaaGAAATCTAAGCGAAGGAAGGCGAACAACAATGTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGc                                             <  2:620768/141‑1 (MQ=255)                                                                                             gaagaagaaATCGAAGCTCTGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGAAAATTTAATCAGTTACATCAACGCTTCAATACTTTGTTTAACAAGcg                                            >  2:136722/1‑140 (MQ=255)                                                                                             gaagaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCa                                            <  1:529537/141‑1 (MQ=255)                                                                                              aagcagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCAc                                           <  1:62649/141‑1 (MQ=255)                                                                                              aagaagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGATGAAAAAACTGAAGAGTAATTCTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCAc                                           <  1:297956/141‑1 (MQ=255)                                                                                                 aagaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgt                                        <  2:334814/141‑1 (MQ=255)                                                                                                  agaaATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACtgtg                                       >  1:233705/1‑141 (MQ=255)                                                                                                   gaaATGGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGc                                      <  2:484032/141‑1 (MQ=255)                                                                                                      aTCGAAGGTAGGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTa                                   <  1:149293/141‑1 (MQ=255)                                                                                                      aTCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTa                                   <  1:510018/141‑1 (MQ=255)                                                                                                        cGAAGCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTAtt                                 <  1:59331/141‑1 (MQ=255)                                                                                                            gCTATGGAAGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTTTGCTTATTTTaa                             >  2:408272/1‑141 (MQ=255)                                                                                                                   aaGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGc                      >  2:218505/1‑141 (MQ=255)                                                                                                                   aaGGCGAACAACAAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACCATGCTTATATTAAAATAAGc                      <  2:505016/141‑1 (MQ=255)                                                                                                                      gCGAACAACAAACCGAAGAACTAGAAGCTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATCAAAGCaag                   <  2:576942/141‑3 (MQ=255)                                                                                                                          accacaaACTGAAGTATGAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATCAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGc               <  1:541936/138‑1 (MQ=255)                                                                                                                          acaacaAACTGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGc               <  2:320580/141‑1 (MQ=255)                                                                                                                          acaacaAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGc               <  1:167806/141‑1 (MQ=255)                                                                                                                             acaaACGGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATGTTAATACAAGCATGGTGCttt            <  1:346984/141‑1 (MQ=255)                                                                                                                                aaCTGAAAAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAATCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTtg         <  2:239892/141‑1 (MQ=255)                                                                                                                                   tGAAGAACTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTAGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTgtgt      <  2:490794/141‑1 (MQ=255)                                                                                                                                      agaaCTAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGttat   >  2:352734/1‑141 (MQ=255)                                                                                                                                       gaaCCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAACGTTTTTATACATTGTTTAACAAGTACTGTGCTTATTTTATTATAAGGAAGTTGCTTTTTGTGttatt  >  2:130337/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                         TTGCTGATGTTAAAGTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCTCCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAAGAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAATTTTAATCTGTTACATTAAAGTTTTTATACTTTGTTTAACAAGCACTGTGCTTATTTTAATATAAGCATGGTGCTTTTTGTGTTATT  >  CP000730/536620‑536894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑