Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9100AG100.0% 164.3 / ‑6.5 49intergenic (‑1/‑121)USA300HOU_0653/USA300HOU_0654ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (23/25);  total (24/25)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAA  >  CP000730/707774‑708042
                                                                                                                                        |                                                                                                                                    
tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACTTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGtt                                                                                                                                  <  2:461407/141‑1 (MQ=255)
 gATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGttt                                                                                                                                 <  1:188791/141‑1 (MQ=255)
          aGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATCAGAAACTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctcct                                                                                                                        >  2:401904/1‑141 (MQ=255)
             tttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAt                                                                                                                     <  2:121640/141‑1 (MQ=255)
                aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                  >  2:296682/1‑141 (MQ=255)
                aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGCTTCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                  >  2:489191/1‑141 (MQ=255)
                 ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaaa                                                                                                                 >  1:174212/1‑141 (MQ=255)
                 ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaaa                                                                                                                 >  2:315540/1‑141 (MQ=255)
                    gCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGAATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTTTTTCTAGCAAACGTTTATACCTCCTAATTAAAAGt                                                                                                              >  1:376422/1‑141 (MQ=255)
                     cACCAGTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGtt                                                                                                             <  2:483709/141‑1 (MQ=255)
                               gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                   <  2:458995/141‑1 (MQ=255)
                               gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                   <  1:659209/141‑1 (MQ=255)
                                   aTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaat                                                                                               <  2:269399/141‑1 (MQ=255)
                                   aTGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaat                                                                                               <  1:612454/141‑1 (MQ=255)
                                             ttattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                                                     <  2:284884/141‑1 (MQ=255)
                                             ttattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                                                     <  2:106306/141‑1 (MQ=255)
                                              tattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataa                                                                                    >  1:46321/1‑141 (MQ=255)
                                                tgtCGCCGTTTAATGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataata                                                                                  <  2:11708/139‑1 (MQ=255)
                                                attCGCCGCGTACTGGTATAGATAAGGAAATGCTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataata                                                                                  <  1:97928/140‑1 (MQ=255)
                                                   cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAAc                                                                               <  2:65244/141‑1 (MQ=255)
                                                          tACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAat                                                                        >  1:122001/1‑141 (MQ=255)
                                                           aCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAata                                                                       >  1:637223/1‑141 (MQ=255)
                                                                          gAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGt                                                        >  1:84781/1‑141 (MQ=255)
                                                                           aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                       <  2:653351/141‑1 (MQ=255)
                                                                                 ttttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                 <  1:325245/141‑1 (MQ=255)
                                                                                        tACATGCTTATTGCCTAAAAACAGAGTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatata                                          >  2:322158/1‑141 (MQ=255)
                                                                                        tACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCACCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGACAATATTTATCATGTCAAGTATATTCGTGatata                                          >  2:372722/1‑141 (MQ=255)
                                                                                                tattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAc                                  >  2:592289/1‑141 (MQ=255)
                                                                                                tattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGACATAATATAGAc                                  >  1:610845/1‑141 (MQ=255)
                                                                                                tattaCCAAAGCACAGATTGAAATCTTTTGTTTCTATGAAGCGATTCTAACTGTCGGCGGTAAGGGTAGGGTAACCTAATTAATGGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATAAAATATAGAc                                  >  2:140903/1‑141 (MQ=255)
                                                                                                  ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAATCATGATATAATATAGACaa                                >  2:122964/1‑141 (MQ=255)
                                                                                                    aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                              <  1:239446/141‑1 (MQ=255)
                                                                                                       tAAAAACATATTTATATCTTTTGATTCTAACAAGCGTTTATACCTCCTAATGAAAAGGTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAGCATGTCAAGTAAATTCGTGATATAATATAGACAatgtat                           <  2:185288/141‑1 (MQ=255)
                                                                                                        aaaaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATAATGTCAAGTAAATTCGTGATATAATATAGACaactaccg                          >  2:548259/1‑135 (MQ=255)
                                                                                                         aaaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                         <  2:637223/141‑1 (MQ=255)
                                                                                                             cAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                     <  1:164164/141‑1 (MQ=255)
                                                                                                             cAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAAACAATGTATGTGAgg                     >  2:334483/1‑141 (MQ=255)
                                                                                                               gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                   >  1:479617/1‑141 (MQ=255)
                                                                                                                aTTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAAGTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGa                  <  1:315540/141‑1 (MQ=255)
                                                                                                                    agaTCTTTTGTTTCTAACAAGCGTTTGTACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAATCTGAGAATATTTATCATGTCAAGTACATTCGTGATATAATATAGACAATGTATGTGTGGCTAAAGt              <  1:489191/139‑1 (MQ=255)
                                                                                                                    aaaTCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGt              <  1:296682/141‑1 (MQ=255)
                                                                                                                       tCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATg           <  2:174212/141‑1 (MQ=255)
                                                                                                                         ttttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGtt         >  1:358194/1‑141 (MQ=255)
                                                                                                                         ttttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGtt         >  1:571829/1‑141 (MQ=255)
                                                                                                                         ttttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGCAAGtt         >  2:484735/1‑141 (MQ=255)
                                                                                                                           ttGTTTCTAACAAGCGATTCTACCTCCTAATTAAAAGTGTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGCCAAGTAAATTCGTGATACAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa       >  1:371728/1‑141 (MQ=255)
                                                                                                                            tGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAAAAAAATAAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATgaaaaa      <  2:46321/141‑6 (MQ=255)
                                                                                                                               ttCTAACAAGCGTTTATATCTCCTAATTAAAAGTTTAGTCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAAGATAGACAATGTATGTGAGGGGAAAGTATGTTAACTga   <  2:162900/141‑1 (MQ=255)
                                                                                                                                tCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAGGTAAGTGAGGTGAAAGTATGTTAACGgaa  >  1:397622/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAA  >  CP000730/707774‑708042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: