Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 756,598 T→A 5.8% intergenic (‑86/‑127) bacA ← / → USA300HOU_0707 undecaprenol kinase/ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730756,5980TA 5.8% 194.7 / 4.8 69intergenic (‑86/‑127)bacA/USA300HOU_0707undecaprenol kinase/ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base T (37/28);  new base A (2/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-01

TAATCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAATTGTGAAAAAATTAAC  >  CP000730/756459‑756738
                                                                                                                                           |                                                                                                                                            
tagtCCTTCGACGACCCCTAAGATAATACCTTTAATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTa                                                                                                                                             <  2:427943/138‑1 (MQ=255)
tAATCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATCCTa                                                                                                                                             >  2:583347/1‑141 (MQ=255)
 aaTCCTTCGACGACCCCTAGGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAg                                                                                                                                            >  1:497573/1‑141 (MQ=255)
 aaTCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAg                                                                                                                                            >  1:400104/1‑141 (MQ=255)
 aaTCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGAAATTCTat                                                                                                                                            >  2:634356/1‑140 (MQ=255)
   tCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGtt                                                                                                                                          <  2:117681/141‑1 (MQ=255)
   tCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGtt                                                                                                                                          <  2:172276/141‑1 (MQ=255)
       tCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTGATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATACTAGTTataa                                                                                                                                      >  1:46528/1‑141 (MQ=255)
       tCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTataa                                                                                                                                      >  1:225166/1‑141 (MQ=255)
          acggcCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATGATAc                                                                                                                                   <  2:7966/141‑1 (MQ=255)
          acgacCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATAc                                                                                                                                   <  1:577948/141‑1 (MQ=255)
           cgacCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACt                                                                                                                                  >  1:534259/1‑141 (MQ=255)
            gacCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCACTACTCAATTTAATTTAGTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTa                                                                                                                                 <  2:236976/141‑1 (MQ=255)
            cacCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTa                                                                                                                                 <  1:309825/140‑1 (MQ=255)
              ccccTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCAAGTTATAATACTAGa                                                                                                                               >  1:259637/1‑141 (MQ=255)
              ccccTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCAAGTTATAATACTAGa                                                                                                                               >  1:516059/1‑141 (MQ=255)
                ccTAATATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACt                                                                                                                             <  2:125876/141‑1 (MQ=255)
                        aataCCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTATAGATGt                                                                                                                     <  2:367418/141‑1 (MQ=255)
                          taCCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGtat                                                                                                                   >  1:221286/1‑141 (MQ=255)
                          taCCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGtat                                                                                                                   <  1:13340/141‑1 (MQ=255)
                              ttttATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTatatat                                                                                                               <  2:331092/141‑1 (MQ=255)
                              ttttATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGAAATGCTAGTTATAATACTAGACTAAAGATGTatatat                                                                                                               <  1:460881/141‑1 (MQ=255)
                                           aTGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTa                                                                                                  <  1:292983/141‑1 (MQ=255)
                                                aaaCATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCCTTTGGTGAGGTGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGt                                                                                             <  1:624770/141‑1 (MQ=255)
                                                   cATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTAACATATCATGATAGCATATTCATTTAAAGACATGCTAGTTGTAATACTAGACTAAAGATGTATATATTAATTTTCTTTTACTTGTaaa                                                                                          >  1:235609/1‑141 (MQ=255)
                                                     tAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAAc                                                                                        >  1:247747/1‑141 (MQ=255)
                                                     tAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAAc                                                                                        >  1:237876/1‑141 (MQ=255)
                                                     tAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGAATAAAGATGTATATATTCATTTTCTTTTACATGTaaaat                                                                                        >  2:609232/1‑140 (MQ=255)
                                                                  ttCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATCTTTTAt                                                                           <  2:367915/141‑1 (MQ=255)
                                                                      ttACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAg                                                                       >  1:115040/1‑141 (MQ=255)
                                                                      ttACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAg                                                                       >  1:538697/1‑141 (MQ=255)
                                                                         cTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTa                                                                    >  2:175853/1‑141 (MQ=255)
                                                                          tCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTAt                                                                   >  1:96824/1‑141 (MQ=255)
                                                                                taatttaTTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCAAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAAtt                                                             <  2:259637/141‑1 (MQ=255)
                                                                                taatttaTTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCAAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAAtt                                                             <  2:516059/141‑1 (MQ=255)
                                                                                 aatttaTTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAAttt                                                            <  2:117922/141‑1 (MQ=255)
                                                                                 aatttaTTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAAttt                                                            <  1:377054/141‑1 (MQ=255)
                                                                                 aatttaTTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAAttt                                                            <  2:596465/141‑1 (MQ=255)
                                                                                  atttatttAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGATCTTTTTAAtttt                                                           >  2:577532/1‑140 (MQ=255)
                                                                                  atttatttAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTg                                                           <  1:175295/141‑1 (MQ=255)
                                                                                   tttatttaAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGa                                                          >  1:309667/1‑141 (MQ=255)
                                                                                    ttatttaAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGAt                                                         >  2:537512/1‑141 (MQ=255)
                                                                                         taAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTaa                                                    >  2:224164/1‑141 (MQ=255)
                                                                                          aaaTATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAg                                                   >  1:543838/1‑141 (MQ=255)
                                                                                          aaaTATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAg                                                   >  1:539228/1‑141 (MQ=255)
                                                                                            atatCAAAATTACCATATAATGATAGCATATTCATTTAAAGACATGCTCGTTATAATACTAGACTAAAGATGTTTATATTCATTTTCTTTTATATGTAATAATACAATATTTTTTTGAGCTATTTAATTTGATTTTAAGGa                                                 >  2:231776/1‑141 (MQ=255)
                                                                                             tatCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGaa                                                >  1:570697/1‑141 (MQ=255)
                                                                                             tatCAAAATTACCATATCATGATAGCATATTCATTTAAAGAAATGCTAGTTATATTACTAGACTAAAGTTGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTTTTTAATTTGTTTTTTAGGaa                                                >  2:265638/1‑141 (MQ=255)
                                                                                                  aaaTTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCt                                           <  2:409406/141‑1 (MQ=255)
                                                                                                     ttACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCtttt                                        >  1:249244/1‑141 (MQ=255)
                                                                                                       aCCTTATCACAATAGTATATTCATTTAATCACATGCTAGTTATAATACTAGACTAAATATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGACAACCTTTtat                                      <  2:214809/141‑1 (MQ=255)
                                                                                                       aCCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTtat                                      >  2:320351/1‑141 (MQ=255)
                                                                                                           tatCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTTAGGAAAACCTTTTataata                                  <  1:399072/141‑1 (MQ=255)
                                                                                                           tatCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTataata                                  <  2:73706/141‑1 (MQ=255)
                                                                                                           tatCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTataata                                  >  1:412416/1‑141 (MQ=255)
                                                                                                            ttCATGATAGCATAGTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAg                                 <  2:620815/140‑1 (MQ=255)
                                                                                                            ataaTGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTTTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAg                                 <  2:363/138‑1 (MQ=255)
                                                                                                                 gATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTa                            >  1:133331/1‑141 (MQ=255)
                                                                                                                 gATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGAAAAACCTTTTATCAATGGTTTa                            >  2:327380/1‑141 (MQ=255)
                                                                                                                     gCATATTCAGTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCAGTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTg                        <  2:69727/141‑1 (MQ=255)
                                                                                                                          ttCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGAttta                   >  2:659556/1‑141 (MQ=255)
                                                                                                                            cATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAAt                 >  2:579332/1‑141 (MQ=255)
                                                                                                                            cATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAAt                 >  1:329093/1‑141 (MQ=255)
                                                                                                                            cATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAAt                 >  1:410071/1‑141 (MQ=255)
                                                                                                                               gtAAATACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACAGGTAAAACTACAATATTTTATTGAGCTATTTAAATTGATTTTAAGTTATACGTTTTATAATAGGTTTAGGTGATATAATtgt              <  2:405660/140‑1 (MQ=255)
                                                                                                                                   aGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTTAGCTATTTAATTTGATTTGAAGGAAAACCTTTTATAATAGGTTTAGGTGATATACTTGTGaaa          >  2:82335/1‑141 (MQ=255)
                                                                                                                                   aGACATGCTAGTTACAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACATTTTATAATAGGTTTAGGTGATATAATTGTGaaa          >  1:347217/1‑141 (MQ=255)
                                                                                                                                       aTGCTAGTTATAATACTAGACTAAAGCTTTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTGTATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAATTGTGAAAAAAt      <  2:269647/141‑1 (MQ=255)
                                                                                                                                         gCTAGTTATAATACTAGACTAAAGATTACTATATTCATTTTCTTTTACATGTAAAACTACAATAAATTATTGCGCTATTTAATTTGATTTTAAGGAAAATCTTTTATAATAGGTTTCGCTAATATAATTGTGAAAAAAtca    >  2:220381/1‑139 (MQ=255)
                                                                                                                                          ttAGTTATTATTATAGACTTCCGATGTATTTTTTTCTTTTTTTTTACATGTAAAAGTACAATTGTTTATTGAGTTTGTTTATTTGATTTTAAGGAAGACCTTTTATAATAGGTTTAGGTGATATAATTGTGAAAAAATTaa   <  2:222522/140‑1 (MQ=255)
                                                                                                                                           tAGTTATAATACTAGACTAAAGATGTATATATTGATTTTCCTGTACATGTAAAACGACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAATTGTGAAAAAATTaac  >  1:319593/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TAATCCTTCGACGACCCCTAAGATAATACCTTTTATTAATTCAATGATAAACATAATGTACCCACTTTCATTACTCAATTTAATTTATTTAAATATCAAAATTACCATATCATGATAGCATATTCATTTAAAGACATGCTAGTTATAATACTAGACTAAAGATGTATATATTCATTTTCTTTTACATGTAAAACTACAATATTTTATTGAGCTATTTAATTTGATTTTAAGGAAAACCTTTTATAATAGGTTTAGGTGATATAATTGTGAAAAAATTAAC  >  CP000730/756459‑756738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: