Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,913 C→G 26.4% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9130CG26.4% 574.6 / inf 459S213S (TCG→TCCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (152/180);  new base G (52/68);  total (206/253)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 462 total aligned reads displayed.

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053
                                                                                                                                            |                                                                                                                                            
cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                                                                                                              >  2:583744/1‑141 (MQ=255)
    aaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaa                                                                                                                                          <  2:162033/141‑1 (MQ=255)
             taaaataaaaTTGTGATCTAATAAAAATAGGAGGTTCTGTTGCAAAGTCAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGGGCAATTGAGTTGCTAGCATGATGCTAACTTCGTGGCAAGGGGAAAATTCGTAGa                                                                                                                                 >  1:453624/1‑141 (MQ=17)
             taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                                                                                 >  1:537439/1‑141 (MQ=255)
                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                      <  1:658730/132‑1 (MQ=11)
                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                      >  1:603793/10‑141 (MQ=11)
                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                      >  2:418945/10‑141 (MQ=11)
                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                  <  1:663003/136‑1 (MQ=11)
                          tattttaataaAAATGATGATTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                  <  1:635151/136‑1 (MQ=11)
                           ccgtcatattaaaTAGCTGGTTCTGTTCCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCtac                                                                                                                  <  1:527764/131‑3 (MQ=11)
                           attttaataaAAAGGATGGTTCTGTTGCAAAGTCAAAAATAAAGATAATTAACTGTTTAGCATGTCAGTGTTCGTTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                 <  1:288862/137‑1 (MQ=2)
                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                <  1:438781/138‑1 (MQ=11)
                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCGTGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                               <  2:555063/139‑1 (MQ=11)
                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGAGCTGAAGAGACCTGCGGt                                                                                                               <  2:144755/139‑1 (MQ=11)
                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                               <  1:106057/139‑1 (MQ=11)
                                                tcagTTCCAAACTAAAAAAACAGCGCTTGCCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCg                                                                                              <  2:520221/138‑1 (MQ=1)
                                                                           aatcACTAATATATCATGTCAGTGTTAGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTa                                                                   <  1:408413/138‑1 (MQ=1)
                                                                               cgtaatatatctgttCATTGTTCCTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGAGCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCt                                                               <  2:540384/127‑1 (MQ=1)
                                                                                        tcttgccAGTTTTCGCTTAACTTGGGAGCATGATGATAATTTGGCGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTGTAGTATTCTTTGCCGTAt                                                      <  2:427353/135‑1 (MQ=1)
                                                                                                gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTGTGTCGAGCGTAAATAGACACAATCCCTTTTAAAGTATTCTTTGCGGTATTTATACtt                                              >  1:285016/1‑141 (MQ=11)
                                                                                                gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              <  1:338898/141‑1 (MQ=17)
                                                                                                gggtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              <  1:158657/139‑1 (MQ=17)
                                                                                                 gtgtCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                             <  2:7907/138‑1 (MQ=11)
                                                                                                  gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTCTTAAAGTATTCTTTGCCGTATTGATACTTTg                                            >  2:566958/1‑141 (MQ=11)
                                                                                                  gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  2:18684/1‑141 (MQ=12)
                                                                                                   ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           <  2:3308/141‑1 (MQ=17)
                                                                                                    tCGTTTAACTTGCTAGCATGATGCTAATTTCGTTGCATTGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  2:381860/141‑1 (MQ=11)
                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                         >  1:139743/4‑141 (MQ=255)
                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAtc                                         >  1:445960/4‑140 (MQ=17)
                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  1:115410/4‑141 (MQ=17)
                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         <  1:570057/138‑1 (MQ=17)
                                                                                                     cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  2:44753/1‑141 (MQ=32)
                                                                                                     cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGCTa                                         >  2:515131/1‑141 (MQ=17)
                                                                                                      gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                        >  1:2255/3‑140 (MQ=11)
                                                                                                      gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAAACTTTGAGAc                                        >  1:617917/3‑141 (MQ=255)
                                                                                                      gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTATTTGCTGTATTGATACTTTGATAc                                        <  1:425297/141‑1 (MQ=17)
                                                                                                       tttAACTTGCTAGGATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                       <  1:304017/140‑3 (MQ=11)
                                                                                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                       <  1:526019/140‑3 (MQ=11)
                                                                                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                       <  1:269088/140‑3 (MQ=255)
                                                                                                        ttAACTTGCTCGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  1:98123/141‑1 (MQ=17)
                                                                                                        ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  1:374002/141‑1 (MQ=17)
                                                                                                        ttAACTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  2:92497/141‑1 (MQ=17)
                                                                                                        acacattGCGAGCATGATGCTAATTACGTGGCGTGGGGAAAATCCGTCGATCTGAAGAGACCTACGGTTCTCTTTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCt                                      >  2:576847/6‑141 (MQ=11)
                                                                                                          aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATAAGTACATCTCATTTGACCTGAGCACAACCAGGTTGAGAGCGTAAATACCCTCCGTACCTTTTAAAGCGTGCTTTGCCGTATTGATACTTTGGTAGCttt                                    >  1:487403/1‑140 (MQ=2)
                                                                                                          aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                    <  2:360559/141‑1 (MQ=17)
                                                                                                           actttgtAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   <  2:30698/135‑1 (MQ=12)
                                                                                                           aCTTGCTTGCATGATGGTAAGTTCGTGGCATGGCGAAAATCCGTAGGTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   <  1:508716/141‑1 (MQ=255)
                                                                                                           aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   >  2:463416/1‑141 (MQ=17)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtt                                  >  2:252492/1‑140 (MQ=255)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATGCTTTGATACCttatt                                  >  2:652326/1‑138 (MQ=12)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  >  1:92909/1‑141 (MQ=17)
                                                                                                            cTTGCTACCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  <  1:261898/141‑1 (MQ=17)
                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTCCCATATTGATACTTTGATATCTTGtctt                                <  2:440825/141‑1 (MQ=11)
                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:101021/141‑1 (MQ=17)
                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:365024/141‑1 (MQ=17)
                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                               >  2:126053/1‑141 (MQ=255)
                                                                                                               gCTAGCATGATGCTAATTTCGTGGCAAGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAAAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               <  1:516191/141‑1 (MQ=12)
                                                                                                                cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                              <  2:471382/141‑1 (MQ=17)
                                                                                                                 tagcgggatgCTAATTTCGTGGCATGGTGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  2:392123/135‑1 (MQ=255)
                                                                                                                 taacATGATGCTAAGTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  2:235651/138‑1 (MQ=11)
                                                                                                                 tAGCATGATGCTAATTTCGTGGCATTGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  2:277342/141‑1 (MQ=11)
                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTGAATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTATCTTGtctttct                             <  1:434024/141‑1 (MQ=11)
                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             >  1:470547/1‑141 (MQ=17)
                                                                                                                 tAGCATGATGCTAATTTCGTGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAccagttgttgctg                            >  2:635040/1‑130 (MQ=11)
                                                                                                                   gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGTTAAATTAATACTTTGTTCCTTTGTTTGTCTTa                           >  2:497720/1‑141 (MQ=11)
                                                                                                                   gCATGATGCCAATTTCGTGGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                           <  1:176352/141‑1 (MQ=12)
                                                                                                                     atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                         <  1:587327/141‑1 (MQ=21)
                                                                                                                      tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                        >  1:356111/1‑141 (MQ=25)
                                                                                                                       gatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                       <  2:377770/141‑1 (MQ=255)
                                                                                                                       gatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                       <  2:346281/141‑1 (MQ=255)
                                                                                                                       gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                       <  2:291389/141‑1 (MQ=25)
                                                                                                                          gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAACGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                    <  1:194375/141‑1 (MQ=25)
                                                                                                                            tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                  >  2:664200/1‑141 (MQ=21)
                                                                                                                            tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                  >  2:485493/1‑141 (MQ=25)
                                                                                                                             aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGTAGAGACCTACGGTTCTTTTGACATAGAGCGTAAATACATTCAATACCTTTTAAAGTTTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                 <  2:366305/141‑1 (MQ=11)
                                                                                                                             aaTTTCGGTGCATTGGGAAAATCCGTTGATGTGAAGATACCTACGGTTCTTTTTATATAGATCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                 <  2:610129/141‑1 (MQ=11)
                                                                                                                              aTTTCGTGGCATGGGGGAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTCTCTTACTGTattctgc                >  1:395323/1‑135 (MQ=11)
                                                                                                                              aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                >  1:250744/1‑141 (MQ=35)
                                                                                                                              aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatataa                >  1:54408/1‑139 (MQ=21)
                                                                                                                               tttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc               <  1:574034/141‑1 (MQ=25)
                                                                                                                                ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg              <  1:232618/141‑1 (MQ=25)
                                                                                                                                ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCGTTCTTACTTTAATATGACg              >  2:201356/1‑141 (MQ=25)
                                                                                                                                  cGTGGCATGGGTAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt            <  2:398141/141‑1 (MQ=14)
                                                                                                                                  cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt            >  2:282985/1‑141 (MQ=38)
                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATCCATTATAAAGTAGGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           >  1:154748/1‑141 (MQ=14)
                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTGTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           >  2:353235/1‑141 (MQ=255)
                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGTg           >  2:370320/1‑141 (MQ=255)
                                                                                                                                     ggCATGGGGAAAAGCCGGAGATCTGAAGAGACCTACGGTTCGTTTTATAGAGAGCGTAAATACATTCAATACCTTTTAAAGGATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt         <  1:218590/141‑1 (MQ=14)
                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         <  1:385892/141‑1 (MQ=39)
                                                                                                                                      gCATGTCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  2:595035/141‑1 (MQ=255)
                                                                                                                                      gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  1:335044/141‑1 (MQ=255)
                                                                                                                                      gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACTTTTTAAAGTATTCTTTGCGGTTTTGATCATTTTATACCTTGTCTTTTTTACTTTAATATGACGGtcacc        >  2:112624/1‑137 (MQ=11)
                                                                                                                                      gCATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  2:265271/141‑1 (MQ=255)
                                                                                                                                        aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  1:564041/141‑1 (MQ=255)
                                                                                                                                         tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:133801/141‑1 (MQ=255)
                                                                                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc    >  1:498870/1‑141 (MQ=37)
                                                                                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCGTACTTTAATATGACGGTGATCTTGc    <  2:642402/141‑1 (MQ=255)
                                                                                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTAAGCTGTATCGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc    >  2:167020/1‑141 (MQ=255)
                                                                                                                                           gCGAAAGTTCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTCTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt   <  2:60269/141‑1 (MQ=255)
                                                                                                                                           gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt   >  1:76823/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: