Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,018,612 G→A 100% R103* (CGA→TGA)  USA300HOU_1869 ← transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,018,6120GA95.1% 117.4 / ‑1.4 41R103* (CGA→TGA) USA300HOU_1869transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (15/24);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATA  >  CP000730/2018475‑2018742
                                                                                                                                         |                                                                                                                                  
aCGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTc                                                                                                                                 >  1:200615/1‑141 (MQ=255)
       ttATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTcc                                                                                                                          >  2:212119/1‑141 (MQ=255)
                 aataatTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaa                                                                                                                <  1:535496/141‑1 (MQ=255)
                  ataatTTTTGACAACGTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATaaa                                                                                                               <  1:98360/141‑1 (MQ=255)
                      tttttGTCAACTTGTTGTGTAATGGTACTATCACCTTGCACAGCTCTGTTGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCa                                                                                                           <  2:515195/141‑1 (MQ=255)
                       ttttGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGTTAATCGTTGAAAATAAAGCTCTAGTTCTACCTTTCAAATCGAATCCATGATGATTGTAGAATCACTCATCTTCCATTGAAATAAAGGCAc                                                                                                          <  2:168999/141‑1 (MQ=255)
                           gACAACTTGTTGTGTAATGGTACTACCACCTTGCCCATCTCTGTCGCTAATCGTTGACAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCttt                                                                                                      <  2:487802/141‑1 (MQ=255)
                                cTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACAt                                                                                                 <  1:552355/141‑1 (MQ=255)
                                cTTGTTGTGTAATGGTACTACCACCTTGCACACCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACAt                                                                                                 <  2:448882/141‑1 (MQ=255)
                                         tAATGGTACTACCACCTTTCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTCTAACATACTCTGGCa                                                                                        <  1:335215/141‑1 (MQ=255)
                                             gggactccCACCTTGCACAGCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGATTCATTCATCTTCCATTGAAATAAATGCACCTTTAACATACTCTGGCATGtt                                                                                    <  2:471619/138‑1 (MQ=255)
                                             ggTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGtt                                                                                    <  2:544364/141‑1 (MQ=255)
                                                actacCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATc                                                                                 >  1:220790/1‑141 (MQ=255)
                                                      accTTGCACATCTCTGTCGCTAGTCGGTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGa                                                                           >  1:490841/1‑141 (MQ=255)
                                                      accTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGa                                                                           >  1:210796/1‑141 (MQ=255)
                                                              caTCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGTCATGTTATCAGCTGACACACAac                                                                   >  2:542705/1‑141 (MQ=255)
                                                                 ctctGTCGCTAATTGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAactac                                                                <  2:281602/141‑1 (MQ=255)
                                                                    tGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACttt                                                             <  1:306196/141‑1 (MQ=255)
                                                                          tcaTCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtttt                                                       <  2:283354/139‑1 (MQ=255)
                                                                          tAATCGTTGAAAATAAAGCTCTAGTTGTACCGTTCAAATCGCATCCATGATGATTGTAGAATCATCCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTAtttt                                                       <  2:604802/141‑1 (MQ=255)
                                                                              cGTTGAAAATAAAGCTCTAGTTTTACCTTTCAAATCGTATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAAt                                                   <  2:26849/141‑1 (MQ=255)
                                                                                ttGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAAttt                                                 <  1:427920/141‑1 (MQ=255)
                                                                                   aaaaTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAACGGCAAAACTTTCAGACTCTGGCATGTTCTCTTCTAACACAAAACTACTTTTATTTTCAATTTTTc                                              <  2:640282/141‑1 (MQ=255)
                                                                                      aTAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTa                                           <  1:212657/141‑1 (MQ=255)
                                                                                       tAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAg                                          <  1:505327/141‑1 (MQ=255)
                                                                                       aaaaGCCCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAg                                          <  1:61797/140‑1 (MQ=255)
                                                                                         aaGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTGTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGtt                                        >  2:295348/1‑141 (MQ=255)
                                                                                             tctAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATc                                    >  2:353149/1‑141 (MQ=255)
                                                                                                  ttGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCCTGTTATCAGCTGACACAATACGACTGTTATTTTCAATTTTTCTTAGTTCATCCACAt                               <  2:294767/141‑1 (MQ=255)
                                                                                                     tACCTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTAt                            >  1:501742/1‑141 (MQ=255)
                                                                                                       ccTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                          <  1:212119/141‑1 (MQ=255)
                                                                                                       ccTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATcg                          >  2:525600/1‑141 (MQ=255)
                                                                                                       ccTTTCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTGTCTTAGTTCATCCACATTATcg                          >  2:358115/1‑141 (MQ=255)
                                                                                                          ttCAAATCGAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACCAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCgt                       >  2:274856/1‑141 (MQ=255)
                                                                                                                  gAATCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCTATTTTTCTTAGTTCATCCACATTATCGCGTGTGGATaa               >  1:243740/1‑141 (MQ=255)
                                                                                                                    aTCCGTGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTAATTTTATTTTACCTCTTCCTTAGTTCCTCCACATTATCGCGTGTAGATaaaa             >  2:138740/1‑141 (MQ=255)
                                                                                                                    aTCCATGATGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaa             <  1:439678/141‑1 (MQ=255)
                                                                                                                      ccATGAAGATTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTGCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATaaaaaa           <  2:235858/141‑1 (MQ=255)
                                                                                                                           atgatTGTAGAATCATTCATCTTCCATTGAAATAAAGGCAACTTTAGCATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACAt      >  1:130158/1‑141 (MQ=255)
                                                                                                                            tgatTGTAGAATCATTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATg     >  1:563427/1‑141 (MQ=255)
                                                                                                                               ttGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATa  >  1:192240/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                  
ACGATCATTATCATAAAAATAATTTTTGACAACTTGTTGTGTAATGGTACTACCACCTTGCACATCTCTGTCGCTAATCGTTGAAAATAAAGCTCTAGTTGTACCTTTCAAATCGAATCCATGATGATTGTAGAATCGTTCATCTTCCATTGAAATAAAGGCACCTTTAACATACTCTGGCATGTTATCAGCTGACACAAAACTACTTTTATTTTCAATTTTTCTTAGTTCATCCACATTATCGCGTGTAGATAAAAAATACATGATA  >  CP000730/2018475‑2018742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑