Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,015 C→T 27.2% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0150CT27.2% 824.4 / inf 559R179R (AGG→AGAUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (218/180);  new base T (81/72);  total (307/252)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 575 total aligned reads displayed.

TTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGAT  >  CP000730/35877‑36154
                                                                                                                                          |                                                                                                                                              
ttAACTTGCTAGCATGATGCTAATTTCTCGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                                                                               <  1:547860/140‑1 (MQ=255)
  aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                                                                             <  2:545751/140‑1 (MQ=33)
   aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTTGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGGGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                                            <  1:253922/140‑1 (MQ=16)
         tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                      >  2:88740/1‑140 (MQ=17)
         tAGCATGATGCTAATATCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                                                                                                      >  1:494992/1‑140 (MQ=12)
          aGCCTGATCGTATTTCCGTGGCATGGCTAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATATAGAGCGTCAATACCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACTTTGATACCTTGtctttct                                                                                                                                     <  2:388916/140‑1 (MQ=11)
           gCATGATGCTAATTTCGTGGCATGGCGACAATCAGGCGCTCTGAAGAGACCTGCTGTGCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACTTTGATACCTTGtctttctt                                                                                                                                    >  2:275006/1‑140 (MQ=11)
           gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                                                                    >  1:29451/1‑140 (MQ=18)
                attgtAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTTCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                               <  2:467755/136‑1 (MQ=11)
                atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                                                                               >  1:558252/1‑140 (MQ=35)
                    tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                                                                                                           >  1:336157/1‑140 (MQ=255)
                     aaTTTGGTGGAATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTTTCTTTCTAGATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTCatat                                                                                                                          <  1:454309/140‑1 (MQ=11)
                      aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAATATg                                                                                                                         >  2:57899/1‑140 (MQ=255)
                           gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAACTACAATCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTATCTTTCTTCCTTTAATATGACgat                                                                                                                    >  1:282007/1‑138 (MQ=11)
                            tGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGATCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACGTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                   <  1:608332/140‑1 (MQ=255)
                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATGTGACGGTg                                                                                                                   >  2:17632/1‑140 (MQ=255)
                            tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                                                                   >  2:44750/1‑140 (MQ=37)
                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATCTAACGGTGa                                                                                                                  >  2:109051/1‑140 (MQ=11)
                             ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                                                                                                  <  2:563362/140‑1 (MQ=255)
                                  ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                            <  1:102097/140‑1 (MQ=255)
                                  ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                                                                            <  1:134736/140‑1 (MQ=255)
                                   gggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                                           <  2:348514/138‑1 (MQ=255)
                                   gCGAAAATCCGTAGATCTGAAGAGACCTGTGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                                                                                                           <  1:392957/140‑1 (MQ=255)
                                    ggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAGGGTGATCTTGCt                                                                                                          >  2:480440/2‑140 (MQ=11)
                                    cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGTTCTTGCt                                                                                                          >  1:323557/1‑140 (MQ=255)
                                    cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                                                                          >  2:24202/1‑140 (MQ=255)
                                       aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                                                                                                       <  2:423481/140‑1 (MQ=255)
                                          tCCGTAGATCTTAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                    <  1:663396/140‑1 (MQ=255)
                                          tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                                                    <  1:536485/140‑1 (MQ=38)
                                            cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAATTTAATATGAAGGTGGTCTTGCTCAATGAgg                                                                                                  >  2:196154/1‑140 (MQ=255)
                                              tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTGGATACCTTGTCTTTCTTACTTTAATATGACGCTGATCTTGCTCAATGAGGtt                                                                                                >  1:382461/1‑140 (MQ=255)
                                              tAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTGATATGACGGTGATCTTGCTCAATGAGGtt                                                                                                <  2:610087/140‑1 (MQ=11)
                                               aGATCTGAAGAGACCTGAGGTTGTTGGTTTATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTTGATGAGGTTa                                                                                               >  2:642569/1‑140 (MQ=11)
                                                   cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa                                                                                           >  2:25979/1‑140 (MQ=255)
                                                    tgatgaGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCTTGTTGCTTGCTCAATGAGGTTATTCAg                                                                                          <  1:659629/140‑1 (MQ=11)
                                                    tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                                          >  2:399241/1‑140 (MQ=255)
                                                    tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                                                                          <  1:588759/140‑1 (MQ=255)
                                                     gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                                                                         <  1:203122/140‑1 (MQ=255)
                                                         agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                                     <  2:122629/140‑1 (MQ=255)
                                                            ccTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGTGGGTATTCAGATATTTCg                                                                                  >  2:62862/1‑140 (MQ=11)
                                                             ctacGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGa                                                                                 >  1:117734/4‑140 (MQ=255)
                                                             cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                                                 >  2:343823/1‑140 (MQ=255)
                                                                cGGTTCTTTTTATCAGGGTCGTAAATACCCTCAATACCTTTTATAGTATTCTTTGCTGTCTTGTGACTTTGATACCTTGACTGTCTTTCTATAATATGACGGTGATCTTGCTCAATGTGGTGATTCAGATATTTctctat                                                                              <  2:281853/140‑6 (MQ=255)
                                                                cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                                              <  1:165207/140‑1 (MQ=38)
                                                                         ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTAGATGTACAATGACa                                                                     >  1:106470/1‑140 (MQ=255)
                                                                          tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                                                                    <  2:264490/140‑1 (MQ=255)
                                                                           atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                                                                   >  1:629438/1‑140 (MQ=255)
                                                                              taGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAg                                                               <  2:116986/140‑1 (MQ=255)
                                                                               agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                              <  1:441791/140‑1 (MQ=255)
                                                                               agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGACATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg                                                              <  2:438705/140‑1 (MQ=255)
                                                                                gagCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATAGCAGTCAGgt                                                             >  1:318550/1‑140 (MQ=255)
                                                                                  gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                           <  1:646304/140‑1 (MQ=255)
                                                                                    gTAAATACACTCAATACCTTTTAAAGTAATCTTTACTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaa                                                         >  1:429026/1‑140 (MQ=14)
                                                                                     tAAATTCACTCAATACCTTTTAGATTAGTCTTTGTTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  2:360658/140‑1 (MQ=14)
                                                                                     tAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTCTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                        >  1:248633/1‑140 (MQ=255)
                                                                                     tAAATACACTCAATACCTTTTAAAGTTTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                        <  2:616684/140‑1 (MQ=255)
                                                                                      aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt                                                       >  1:473959/1‑140 (MQ=255)
                                                                                       aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                      >  2:622599/1‑140 (MQ=255)
                                                                                       aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                      >  1:400025/1‑140 (MQ=255)
                                                                                        aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                     >  2:75918/1‑140 (MQ=255)
                                                                                        aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGCTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                     >  1:448303/1‑140 (MQ=255)
                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  1:62313/1‑140 (MQ=40)
                                                                                         tACATTCAATACCTTTTAAAGTATTCTTTGCCGTGTTGATACTTTCGTATATTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                    >  1:157335/1‑140 (MQ=14)
                                                                                         tACATTCAATACCTTTTAAACTATTCTTTGCTGTATTGATACTTTGATACCTCGTCTTGCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGCACAATGACAGTCAGgtttaagttta                                                    >  1:406504/1‑140 (MQ=255)
                                                                                          acactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                   <  1:568224/136‑1 (MQ=255)
                                                                                           cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaa                                                  >  1:26686/4‑140 (MQ=255)
                                                                                            actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                 >  2:205792/3‑140 (MQ=255)
                                                                                            actCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                 >  2:595728/3‑140 (MQ=255)
                                                                                             ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATGTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAg                                                <  1:188841/140‑1 (MQ=255)
                                                                                              ccAATACCTTTTAAAGTATTCTTAGCAGTCTTGATATTATGATAGCCTGTATTTACTCCTTTAATGTGAAGGTGATCTAGCTCATACTGGTTATTCATATATTTCAATTTACAATGCAAACACGGTTTAAGTTTAAAAgt                                               >  2:296844/2‑139 (MQ=2)
                                                                                               cAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTATTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCt                                              >  1:145644/1‑140 (MQ=255)
                                                                                                  tatcTTTTAAAGTATTCTTTGCTGGATTGATACTTTTATTCCTTGTCGTTCTTACTTTAATATGACGGTGATCTTGTTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                                           <  2:302571/137‑1 (MQ=14)
                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGCTATTTCGACGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                                          <  2:333115/140‑1 (MQ=255)
                                                                                                   aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                                          >  1:12067/1‑140 (MQ=255)
                                                                                                    ccTTTTAAAGTATCCTTTGCCGTATCGATACTTTGATCTCTTCTCTTTCTTACTTCAATATGACGGTGATCTTGCTTAATGACGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                         <  2:37029/140‑1 (MQ=14)
                                                                                                     cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                        <  2:508258/140‑1 (MQ=255)
                                                                                                      ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:213982/1‑140 (MQ=255)
                                                                                                      ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                       >  2:410901/1‑140 (MQ=40)
                                                                                                            aGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaa                                 >  1:238360/1‑139 (MQ=255)
                                                                                                               aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCa                              <  2:663097/140‑1 (MQ=255)
                                                                                                                   tttgcgctattctcaCTGTGTTACCTTGTCTGTCTTTCTTTAATAGTACGGTGATCTTTCTCGATGGGGTTATTTACATATTTCTATGTACACTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAAGCATTGc                          <  2:165345/126‑1 (MQ=2)
                                                                                                                   tttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCACTTGc                          >  1:81815/1‑140 (MQ=14)
                                                                                                                    ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCt                         >  2:230248/1‑140 (MQ=255)
                                                                                                                     tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa                        >  1:369070/1‑140 (MQ=255)
                                                                                                                     tGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTa                        >  2:156886/1‑140 (MQ=255)
                                                                                                                       cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTGTAACCATTGCTAcc                      >  1:582384/1‑140 (MQ=255)
                                                                                                                         gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGACGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCta                    >  1:304001/1‑139 (MQ=255)
                                                                                                                           aTTGATACTTCGATACCTTGTCTTTCTTACTTTAATATGACGTTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCg                  <  2:518698/140‑1 (MQ=255)
                                                                                                                            ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 >  1:504411/1‑140 (MQ=255)
                                                                                                                            ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 >  1:599234/1‑140 (MQ=255)
                                                                                                                            ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                 >  1:530130/1‑140 (MQ=255)
                                                                                                                               aTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAGCATCGATGa              >  2:135870/1‑140 (MQ=14)
                                                                                                                                 aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGTTTATTCATATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTTATAGCTTTAATTACTTTAATCATTGCTACCTTCGTTGagg            >  1:98797/1‑138 (MQ=14)
                                                                                                                                  cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg          <  2:323551/140‑1 (MQ=255)
                                                                                                                                   ctgtaTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt         <  2:494992/136‑1 (MQ=255)
                                                                                                                                     tGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc       <  1:416145/140‑1 (MQ=255)
                                                                                                                                     tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc       <  1:523047/140‑1 (MQ=255)
                                                                                                                                       atatCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCt     <  2:529563/140‑1 (MQ=255)
                                                                                                                                         aCCTTGTCTTTCTTACTTTAATCTGACGGTGATCTTGCTCAATGAGGTTATTCAAATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTGGTGAAGAGTGCCTGa   >  1:215886/1‑140 (MQ=11)
                                                                                                                                          ccTTGTCTTTCTTAGTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt  >  1:364441/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                              
TTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGAT  >  CP000730/35877‑36154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: