BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	28.5%	I131V (ATT→GTT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	28.5%	494.9 / inf	469	I131V (ATT→GTT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (172/162); new base C (68/66); total (240/229)
Fisher's exact test for biased strand distribution p-value = 9.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 475 total aligned reads displayed.

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTT  >  CP000730/36022‑36300

tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAt                                                                                                                                             >  2:318896/1‑140 (MQ=37)

  tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCATTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTa                                                                                                                                           >  1:522664/1‑140 (MQ=255)

  tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTa                                                                                                                                           <  1:612850/140‑1 (MQ=38)

   cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAc                                                                                                                                          <  2:18135/140‑1 (MQ=255)

    ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAcc                                                                                                                                         <  1:254109/140‑1 (MQ=255)

     tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCt                                                                                                                                        >  2:561851/1‑140 (MQ=255)

     tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTGCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGAGCTGTAACTGCCt                                                                                                                                        >  2:430459/1‑140 (MQ=14)

      aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCGGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtt                                                                                                                                       >  1:635532/1‑140 (MQ=255)

      aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtt                                                                                                                                       >  1:666491/1‑140 (MQ=255)

      aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtt                                                                                                                                       >  1:144603/1‑140 (MQ=37)

        tttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGCGCCTGCTCTGTAATTCCCtttt                                                                                                                                     >  2:117107/1‑140 (MQ=11)

        tttAATATGACGGTGATCTTGCCCAATGAGGTTATTCAGATATTTAGATGTACAATGGCAGTCAGGTGTAAGCTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGCGCCTGCTCTGTAATTACCtttt                                                                                                                                     >  2:604522/1‑140 (MQ=11)

          tactatGACGGTGATCTTGCTCATTGAGGTTATTCAGATATTTAGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAATCATTGCTACCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                                                   <  2:151881/137‑1 (MQ=11)

          tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGa                                                                                                                                   >  1:506653/1‑140 (MQ=255)

          tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                                                                                                   <  2:339252/140‑1 (MQ=37)

            ctatGTCGGTGTACTTGCTCCATGAGGTTATTCAGATATGTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                                                 <  2:457825/139‑1 (MQ=11)

            atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAAGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                                                 >  1:94674/1‑140 (MQ=255)

            atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                                                 <  1:493387/140‑1 (MQ=34)

             tatGACGGTGATCTTGCTCAATGAGATTATTCAGATTTTTCGATGTACAATGACAGTCCGGTTTAAGTTTAAAAGCTTTAATTATTGTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggc                                                                                                                                >  1:659672/1‑139 (MQ=11)

              atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                                               <  2:330376/140‑3 (MQ=255)

               tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGctt                                                                                                                              >  1:169621/1‑140 (MQ=255)

                 accgTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                            <  1:444874/137‑1 (MQ=12)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTATCTTTGTTGTAGATGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                            >  2:221075/1‑140 (MQ=11)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                            <  1:437498/140‑1 (MQ=21)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                                            <  2:64426/140‑1 (MQ=11)

                   ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTCAAGTTTAAAAGCTTTAATTATTTTAGCAATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCCTTTGAGGCTTACCa                                                                                                                          >  1:126638/1‑140 (MQ=11)

                     tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTTAAGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTATCTGCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                        <  1:157540/140‑1 (MQ=11)

                     tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                        <  1:468455/140‑1 (MQ=11)

                     tGATCTTGCTCAATGAAGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                        >  1:330116/1‑140 (MQ=18)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCTTTTGAGGTTTACCAAATTg                                                                                                                     >  2:594796/1‑140 (MQ=255)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                     >  1:105324/1‑140 (MQ=16)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                     >  1:233684/1‑140 (MQ=16)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                     >  1:134703/1‑140 (MQ=16)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                     >  2:591371/1‑140 (MQ=16)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTg                                                                                                                     >  1:549049/1‑140 (MQ=11)

                         cTTGCTCAATGAGGTTCTTCAGATATTTCGATGTACAATGACAGTCAGGTTTAATTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                    <  1:394492/140‑1 (MQ=17)

                         cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                    >  2:297891/1‑140 (MQ=16)

                         cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGACTGATCTGTAATTACCTTTTGAGGTTTACCAAGTTGt                                                                                                                    >  1:192272/1‑140 (MQ=17)

                          ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                   >  1:542351/1‑140 (MQ=16)

                             cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTCATCTGTAATTACCTTTTGCGGTTTACCAAATTGTTTaa                                                                                                                <  1:371398/140‑1 (MQ=255)

                              ttgaTGAGGTTATTCACATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                               <  2:446420/137‑1 (MQ=255)

                               cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                              <  2:257702/140‑1 (MQ=12)

                                 aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                            <  2:352590/140‑1 (MQ=12)

                                     ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                        <  1:199372/140‑1 (MQ=255)

                                         aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAATTTAAAAGCTTTAATTACTTTAACCATTGCTAACTTCGTTGAAGGTGCCTGATCTGTAATTATCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                    >  2:198114/1‑140 (MQ=11)

                                          ttCAGAAATTTCGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTTATTTCTTTAATCATTGCTACCTTCGTTGAAGGTGTGTGTTATGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                   <  1:41030/140‑1 (MQ=11)

                                            cAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                 <  1:349203/140‑1 (MQ=17)

                                              gATATTTCCATGTACAATGACAGGCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGCAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                               <  2:547201/140‑1 (MQ=11)

                                               atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                              <  1:308033/140‑1 (MQ=17)

                                               atatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                              <  1:281408/140‑1 (MQ=11)

                                                 atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                            <  1:228597/140‑1 (MQ=11)

                                                   ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata                                                                                          >  1:628640/1‑140 (MQ=16)

                                                    tCGGTGTACTATGGAAGTCAGGTTTAAGTTTAAAAGCTTTACTTGCGTTTACTATTGCTACCTTCGTTGAAGGTGCATGATCTGTAATTACCTTTTGATGTTTACGATATTGTTTAATGGGACGTTTGATAAACGCatat                                                                                         <  2:215886/140‑1 (MQ=11)

                                                    tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATGAGACGTTTGATAAACGCatat                                                                                         <  2:473959/140‑1 (MQ=16)

                                                      gATGTACAATGGCAGTCAGGTTTAAGTTTCAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGCTCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                       >  1:295654/1‑140 (MQ=11)

                                                         gTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                    <  1:109051/140‑1 (MQ=17)

                                                         gTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                    <  2:284000/140‑1 (MQ=255)

                                                          tACAATGGCAGTCAGGTTTAAGTTTAACAGCTTTGATGACTTTAAACATTGCTATCTTCGTTGAAGGTGCCTGTTCTGTAATTACCTTTTGAGGTTTCCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                   <  1:585565/140‑1 (MQ=11)

                                                          tACAATGGCAGTCAGGTTTAAGTTTAAAAGTTTTAATTACTTTAACCATTGCTACCTTCTTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                   <  1:380837/140‑1 (MQ=11)

                                                          tACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                   >  2:76923/1‑140 (MQ=16)

                                                          tACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                   >  2:581160/1‑140 (MQ=16)

                                                           aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                  <  1:75918/140‑1 (MQ=11)

                                                            cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                 >  1:272189/1‑140 (MQ=9)

                                                                gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                                             >  2:232537/1‑140 (MQ=255)

                                                                                   aataGCTTTAATTACTTTAACTATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAAcc                                                          <  1:86896/137‑1 (MQ=0)

                                                                                                    taaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACACATATGCTGAATGATTATCTCGTTGCTTACACAACCAAATATCTAatgtatgt                                         >  1:162483/4‑140 (MQ=9)

                                                                                                     aaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAACTTGTTTAATGAGACGTTTGCTAAACGCATATGCTGAATGATTATCTGGTTGCTTACGCACCCAAATATCTAATGTATGTc                                        >  2:24787/3‑140 (MQ=11)

                                                                                                        cATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct                                     <  2:403225/140‑1 (MQ=255)

                                                                                                         aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                    >  2:380742/1‑140 (MQ=32)

                                                                                                         aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                    >  1:579442/1‑140 (MQ=32)

                                                                                                         aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                    <  2:530130/140‑1 (MQ=33)

                                                                                                          ttGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAGTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                   >  1:573442/1‑140 (MQ=255)

                                                                                                          ttGCTACCTTCGTTGAAGGTGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                   >  1:427404/1‑140 (MQ=17)

                                                                                                          ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCACCCAAATATCTAATGTATGTCCctct                                   >  2:651525/1‑140 (MQ=17)

                                                                                                             cTACCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                <  2:629438/140‑1 (MQ=18)

                                                                                                              tACCTTCGTTGAAGGTGCCTGATCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAAAAGCAAATGAATGTCCttccgtac                               >  2:494185/1‑135 (MQ=11)

                                                                                                              tACCGTCGTTGAGGGTGCCTGTTCGGTAATTACTTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                               <  2:519602/140‑1 (MQ=11)

                                                                                                               aCCTTCGTTGAAGGTGCCTGATATGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTACGGCACTCTGCATc                              >  2:41325/1‑140 (MQ=255)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTTCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                             >  1:179167/1‑140 (MQ=17)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                             >  1:357875/1‑140 (MQ=33)

                                                                                                                ccTTAGTTGAAGGTGCCTGATCTGTAATTACCGTTTGAGGATTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                             >  1:476748/1‑140 (MQ=12)

                                                                                                                   tCGTTTAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                          <  1:622705/140‑1 (MQ=255)

                                                                                                                    ctttGAAGGGGCCTGATCTGTAATTACCTTTTTAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATATCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                         <  2:141332/138‑1 (MQ=11)

                                                                                                                     gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGc                        <  2:12067/140‑1 (MQ=32)

                                                                                                                     gTTCAAGGTGCCTGATCTGTAATTACCGTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGc                        <  1:563588/140‑1 (MQ=255)

                                                                                                                         aaGGTGTCTGATCTGTATCTATCTTTTGAGGCTTATCAAATTGTTTAATGAGACGTTTGATAATCGCATATTCTGAATGATTATCCCGTTGCTTACGCAACCAAATATCTAATGTTTGTCCCTCTGTATCAATGGCACGa                    <  1:486950/140‑1 (MQ=11)

                                                                                                                          aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATAGGCTGAATGATTATCTCGTTACTTACGCAACCAAATATATCATGTATGTACCTCTGCAACAATTGCAGGaa                   >  2:302444/1‑139 (MQ=11)

                                                                                                                            gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatat                 <  1:211275/140‑1 (MQ=33)

                                                                                                                             tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATATCGTTGCTTACGCAAGCAAAGATATAATGTATGTCCATCTGATTCAATGTAACGAtata                >  2:61679/1‑140 (MQ=11)

                                                                                                                               ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaaa              >  2:365747/1‑140 (MQ=32)

                                                                                                                               ccTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTATGTCCCTCTGTATCAATGGCACGATATaaa              >  2:46657/1‑140 (MQ=255)

                                                                                                                                 tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa            >  1:412886/1‑140 (MQ=255)

                                                                                                                                 tGATCTGTAACGACCTTTTGAGGCTTACGAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAGTa            <  2:659672/140‑1 (MQ=11)

                                                                                                                                  gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGCTTATCTCGTTTCGTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg           >  1:654596/1‑140 (MQ=255)

                                                                                                                                    tCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt         <  2:506166/140‑1 (MQ=255)

                                                                                                                                     cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc        <  1:532085/140‑1 (MQ=18)

                                                                                                                                     cTGTAACTCCCTTTTGAGGCTTACCAAATTGTTTAATGAGTCTTTTGATAACCGCATATGTTGAATGATTATCTCGTTTCTTACGCAACCAAATATCTAATTTATGTCCCTCGGTATCAATGGCACTATATAAATAGCTc        <  2:276510/140‑1 (MQ=11)

                                                                                                                                      tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc       <  2:238360/140‑1 (MQ=32)

                                                                                                                                        tAATTACCTTTTGAGGTTTACCAAATTTTTTAATGAGACGTTTGGTCCTCTCTCTTTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt     <  1:151548/140‑1 (MQ=11)

                                                                                                                                         aaTTACCTTTTGAGGTTTTCCAAATTGTTTAATGAGACCTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtt    <  2:539737/140‑1 (MQ=17)

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTT  >  CP000730/36022‑36300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`