BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	86.9%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	86.9%	771.0 / 118.5	469	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (26/36); new base T (200/203); total (230/239)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 470 total aligned reads displayed.

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAT  >  CP000730/36240‑36517

 aaCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                                                                                           <  2:644537/140‑1 (MQ=38)

  tccAAATATCTAATGTTTGTTCCTCTGTATCAATGGCACGATATAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                                                                                          <  1:328770/139‑1 (MQ=11)

  aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCTCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACaaa                                                                                                                                          <  1:318896/140‑1 (MQ=255)

   ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCTTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                         <  1:117107/140‑1 (MQ=255)

    cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTTAGAAGAATTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                        >  1:513952/1‑140 (MQ=11)

      aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTAACATTg                                                                                                                                      >  2:495713/1‑140 (MQ=11)

       atatCTAATGTATGTCCTTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                     <  2:182427/140‑1 (MQ=255)

       acagctgatgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTCGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                     <  2:36731/133‑1 (MQ=11)

             aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                                                                               <  1:245064/140‑1 (MQ=255)

             aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATTTAATATAACCTTAGGGctaa                                                                                                                               >  2:161809/1‑137 (MQ=11)

              atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTACATATTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                                                                                              >  2:436918/1‑140 (MQ=255)

               tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTTTTCCAAATTTGATATAAAATTGGGGCatcta                                                                                                                             >  1:143622/1‑137 (MQ=11)

               tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTTTCATCAATACGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAAATTGATATAAAATTGGGGCATAtt                                                                                                                             >  1:560619/1‑140 (MQ=11)

                  atgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt                                                                                                                          >  2:63110/1‑140 (MQ=38)

                   tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTg                                                                                                                         >  1:534056/1‑140 (MQ=255)

                   tgtCACTCTGCATCAATGGCACGATATAAATAGGAACATTGTCCTTTTATTTTGATGTCCGTATCATCAATAAGCGATTTGTAAGAAGGTTTTTTATGCTTTTTCTTCCAAATAGAAGCTAAAATTGCGGCGTGTTCTTg                                                                                                                         >  2:213389/1‑140 (MQ=2)

                    gtCCCTCTTTATCAATGGCACGATATAAATATCTCCATGTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                                                                                        <  1:465710/140‑1 (MQ=11)

                     tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                       >  2:478979/1‑140 (MQ=21)

                          ctgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                  >  2:173955/5‑140 (MQ=255)

                          ctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAATCCaa                                                                                                                  >  1:622724/1‑140 (MQ=255)

                             taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTTCTCTAAAATTACGGCATCTTTTTGactctttgct                                                                                                               >  1:150536/2‑131 (MQ=11)

                             taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                               <  1:65136/139‑1 (MQ=16)

                             taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                               >  2:312436/2‑140 (MQ=255)

                             cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                               <  1:364763/140‑1 (MQ=18)

                              aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGGTTTTGCTTCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                              >  2:638185/1‑140 (MQ=11)

                                cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCAAAAGGTAg                                                                                                            >  1:347657/1‑140 (MQ=11)

                                 actGGCACGATATAAATACTTCTATTTTCCTTTTATTTTGATATATGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATTCTTGTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGa                                                                                                           <  2:628640/138‑1 (MQ=11)

                                    tgCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                        <  1:634560/139‑1 (MQ=18)

                                     gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                       <  2:450152/140‑1 (MQ=255)

                                      cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                      >  1:377702/1‑140 (MQ=255)

                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                     >  1:528791/1‑140 (MQ=255)

                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                     >  1:479112/1‑140 (MQ=255)

                                        cGATATAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                    <  2:48952/140‑1 (MQ=11)

                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                    >  1:603510/1‑140 (MQ=25)

                                            ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                <  2:408911/140‑1 (MQ=255)

                                             taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTGGACCGTTGAatgat                                                                                               <  2:452989/140‑1 (MQ=255)

                                             taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                               >  1:238441/1‑140 (MQ=255)

                                             taAATAGCTCAATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTTTTGAGCCCAACGGTAGAGCGTTGAatgtt                                                                                               >  2:467039/1‑138 (MQ=11)

                                              aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgata                                                                                              >  1:546678/1‑139 (MQ=18)

                                               aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                             <  2:2944/140‑1 (MQ=255)

                                                  atcTCCCTTTTTCTTTTATTTGGATTGTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                                                                                          <  2:340089/138‑1 (MQ=11)

                                                  aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                                                                                          >  1:128669/1‑140 (MQ=40)

                                                      ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                      <  1:391262/140‑1 (MQ=255)

                                                      ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                      >  1:592689/1‑140 (MQ=255)

                                                       cATTTTCCTTTCATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                                                                                     >  2:209531/1‑140 (MQ=255)

                                                        aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                    >  1:335699/1‑140 (MQ=255)

                                                        aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATCGTTGAATGATGAACGTTTaca                                                                                    >  1:180835/1‑140 (MQ=255)

                                                          tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                  <  1:105399/140‑1 (MQ=18)

                                                           tttgtttttgttttATGGACGTTTCATCAATAAGCAATAGGTAAAAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                 <  2:68930/130‑1 (MQ=11)

                                                           ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                 >  1:251448/1‑140 (MQ=255)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                               <  2:587180/140‑1 (MQ=21)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                               <  1:509378/140‑1 (MQ=40)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGAGGCATATTCTTGAACCAAAAGGTAGAAAGTTTAATGATGAACGTATACACCACg                                                                               >  1:283526/1‑140 (MQ=14)

                                                             ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                               >  1:215115/1‑140 (MQ=255)

                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                              <  2:272189/140‑1 (MQ=255)

                                                               ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                             >  2:119017/1‑140 (MQ=25)

                                                                   aTTTTGATGTACGTCTCAGCGATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCGTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                         <  2:545590/140‑1 (MQ=255)

                                                                    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  2:654941/140‑1 (MQ=11)

                                                                    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  1:580999/140‑1 (MQ=11)

                                                                    ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  2:573442/140‑1 (MQ=11)

                                                                    ttttGAGATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  2:190220/140‑1 (MQ=11)

                                                                    ttgggttacaCGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  2:126638/131‑1 (MQ=11)

                                                                      ttGATTTACGTCCTATTAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                      <  1:300642/140‑1 (MQ=11)

                                                                       tGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTTTGTTTTTTTTTCCAAATTTGTTATAAAATTGAGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat                                                                     <  1:92013/140‑1 (MQ=11)

                                                                       tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGACGAACGTTTACACCACGTTCTCTTAat                                                                     <  2:412886/140‑1 (MQ=11)

                                                                        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                    >  2:284240/1‑140 (MQ=21)

                                                                        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                    >  2:357953/1‑140 (MQ=25)

                                                                          tatACGTCTCATCAAGACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACTCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                  <  2:227383/138‑1 (MQ=11)

                                                                             aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                               <  1:27618/140‑1 (MQ=255)

                                                                             aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCCTGAACCCAACGCTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                               <  2:39199/140‑1 (MQ=255)

                                                                              cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTGCACCACGTTCTCTTAATATTTCAg                                                              >  2:611476/1‑140 (MQ=255)

                                                                                 ctcAACAATACCCCATTTGTAATAAGCTCTTATATGCTATTTCTTCCAAATTTTATATAATATTGGGGTATATTCTTTAACCAAAATGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGata                                                           <  1:221682/140‑1 (MQ=11)

                                                                                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                         <  1:383038/140‑1 (MQ=255)

                                                                                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                        >  1:610648/1‑140 (MQ=255)

                                                                                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                      <  1:192247/140‑1 (MQ=21)

                                                                                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                      <  1:450087/140‑1 (MQ=21)

                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                     <  1:564137/140‑1 (MQ=255)

                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGCTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                     <  1:662027/140‑1 (MQ=255)

                                                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACGCCACGTTCTCTTAATATTTCAGATATATCACGa                                                   >  2:66396/1‑140 (MQ=255)

                                                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                   >  2:508945/1‑140 (MQ=255)

                                                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                   >  1:574908/1‑140 (MQ=21)

                                                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATATGGGCATATTCTTGAACCCAACGGTAGATAGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATAAATCACGa                                                   >  1:422022/1‑140 (MQ=11)

                                                                                            gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                >  2:5302/1‑139 (MQ=255)

                                                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCGAAAGGAATTCTGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATGTATTACGATAAc                                               >  2:569293/1‑140 (MQ=11)

                                                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTATTATTTCATATATATCACGATagc                                               >  2:520696/1‑138 (MQ=11)

                                                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                               <  2:240534/140‑1 (MQ=18)

                                                                                              cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagct                                              >  1:459466/1‑137 (MQ=11)

                                                                                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGTTTTCTTAATATTTCAGATATATCCCGATAGCtt                                             >  2:93292/1‑139 (MQ=11)

                                                                                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                          <  1:492450/140‑1 (MQ=18)

                                                                                                   taaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                         <  2:606692/138‑1 (MQ=255)

                                                                                                   gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                         <  1:2620/140‑1 (MQ=11)

                                                                                                   gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCATg                                         >  2:1552/1‑140 (MQ=19)

                                                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGt                                        >  2:252146/1‑140 (MQ=255)

                                                                                                     aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                       <  1:169307/140‑3 (MQ=11)

                                                                                                     aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGGACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTGAGATATATCACGATAACTCAATgca                                       >  1:407767/1‑138 (MQ=255)

                                                                                                     aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                       <  2:619212/140‑3 (MQ=17)

                                                                                                     aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAGTTATTCCGGTACCTCAATgca                                       >  1:312750/1‑138 (MQ=11)

                                                                                                      ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTAATCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCTTGtat                                      >  1:303678/1‑140 (MQ=255)

                                                                                                                  aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                          <  2:105841/140‑1 (MQ=35)

                                                                                                                  aTGCTTCTTCTTCCAAATTTGAGACAAAAGTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAGGTATATCTTAGAtagt                          <  1:438547/140‑1 (MQ=11)

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAT  >  CP000730/36240‑36517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`