BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,413	C→T	49.0%	V47I (GTC→ATC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,413	0	C	T	49.0%	78.8 / inf	369	V47I (GTC→ATC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (90/93); new base T (92/87); total (188/181)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 369 total aligned reads displayed.

GGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCA  >  CP000730/36276‑36550

tgCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                         <  1:634560/139‑1 (MQ=18)

 gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGt                                                                                                                                        >  1:442741/1‑140 (MQ=40)

 gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                                                        <  2:450152/140‑1 (MQ=255)

  cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                                                                                                                       >  1:377702/1‑140 (MQ=255)

   aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                                                      <  1:581160/140‑1 (MQ=21)

    cGATATAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                                                     <  2:48952/140‑1 (MQ=11)

    cGATATAAATAGCTCCTTTCTCCTTCTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                     <  1:647661/140‑1 (MQ=255)

      atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTGGATTGTTGAat                                                                                                                                   >  1:606692/1‑140 (MQ=11)

       tgtaAAGAGCTCCATTTTCCTTGTATTGTGATGTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                  <  2:608373/138‑1 (MQ=11)

       tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                  <  2:162483/140‑1 (MQ=21)

        ttaACTAGCTCCATTTTCCGTTTATTTTCATATCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                 <  2:62489/139‑1 (MQ=11)

        ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                 <  2:408911/140‑1 (MQ=255)

         taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                                                                >  1:238441/1‑140 (MQ=255)

         taAATAGCTCAATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTTTTGAGCCCAACGGTAGAGCGTTGAatgtt                                                                                                                                >  2:467039/1‑138 (MQ=11)

          aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgata                                                                                                                               >  1:546678/1‑139 (MQ=18)

          aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                                               <  2:92313/140‑1 (MQ=255)

          aaaTAGCAACATATGCCATTTATTATGATGTACGTCCCATCAATAAACCATTTGTAATAAGCTCTTTTATGCCTTTTCTTCCAAATTTGCTATAAAATTGAGGCATATTCTTGAACCCAACGGTAGACCGTTTAatgatg                                                                                                                               >  2:453189/1‑140 (MQ=11)

          aaaGAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                                                               >  1:479237/1‑140 (MQ=25)

           aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                                              <  2:2944/140‑1 (MQ=255)

            aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                             >  2:630139/1‑140 (MQ=255)

             tAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGCTGAATGATGAAc                                                                                                                            >  2:261050/1‑140 (MQ=11)

              aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                                                                                                                           >  1:660779/1‑140 (MQ=25)

              aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACg                                                                                                                           >  1:112458/1‑140 (MQ=21)

                ctgcATTTGCCGTTTTTGTGGATATATGTCTCATGTATAGGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                         <  2:509691/137‑1 (MQ=11)

                  cctttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAGTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                                                       <  2:177340/137‑1 (MQ=11)

                   cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                                                                                                                      <  1:382890/140‑1 (MQ=18)

                    aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                     >  1:335699/1‑140 (MQ=255)

                    aTTTTCCTTTTATTTTGATAGACGTCTCATCAATACGCCATTTGCAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                     >  2:364152/1‑140 (MQ=11)

                     ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                    <  2:237841/140‑1 (MQ=40)

                     ttttCCTTTGATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTCCCACATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                    <  2:73693/140‑1 (MQ=255)

                      tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                   <  1:105399/140‑1 (MQ=18)

                      tttCCTTTTATTTTGATGTACGGCTCATCAATACGCCATTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                   <  2:298321/140‑1 (MQ=16)

                       ttCCTTTTATTTTGATATACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                  <  1:314792/140‑1 (MQ=255)

                        tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                                                 <  2:493588/140‑1 (MQ=40)

                        tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGCGTGACGATTGAAGCCAACGGTAGACCGTTCAATGATGACAGTTTAccccac                                                                                                                 >  2:638164/1‑140 (MQ=14)

                         ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGAGGCATATTCTTGAACCAAAAGGTAGAAAGTTTAATGATGAACGTATACACCACg                                                                                                                >  1:283526/1‑140 (MQ=14)

                         ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                                >  1:215115/1‑140 (MQ=255)

                          cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                               <  1:596982/140‑1 (MQ=255)

                           ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                              >  2:119017/1‑140 (MQ=25)

                             ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                                                            >  2:658540/1‑140 (MQ=21)

                              tATTTTGATGTACGTCTTCTCAATACGCCATTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                                           <  1:578253/140‑1 (MQ=255)

                                ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGTTGAACGTTTACACCACGTTCCCtt                                                                                                         >  1:415688/1‑140 (MQ=25)

                                ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                         <  1:31153/140‑1 (MQ=21)

                                ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTGATATAAAATCGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                         <  1:49913/140‑1 (MQ=11)

                                ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGTATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                         <  1:156159/140‑1 (MQ=11)

                                  ttGATTTACGTCCTATTAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                       <  1:300642/140‑1 (MQ=11)

                                   tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                                                      <  1:664785/140‑1 (MQ=18)

                                   tGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTTTGTTTTTTTTTCCAAATTTGTTATAAAATTGAGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat                                                                                                      <  1:92013/140‑1 (MQ=11)

                                   tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGACGAACGTTTACACCACGTTCTCTTAat                                                                                                      <  2:412886/140‑1 (MQ=11)

                                    gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAata                                                                                                     >  1:3546/5‑140 (MQ=11)

                                    gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                                                     >  2:478917/5‑140 (MQ=11)

                                    gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                                                     >  1:325967/5‑140 (MQ=11)

                                    gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTAATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATTGTTGAATTATGAAAGTTTACACCACGTTGTCTTAata                                                                                                     >  1:385211/5‑140 (MQ=11)

                                    gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                                                     >  2:578867/1‑140 (MQ=25)

                                    caTGTACGTCTCTTCAAACCGCCATGTGTAATAAGCTTCTTTATGCTTTTTCTTTCAAGATTGAGATAAAATAGAGCCATATTTTTGAACCGCACTTTACACCGTTGAATGATGAACGTTTACACCACGTTCCCTCAata                                                                                                     >  2:219227/2‑140 (MQ=2)

                                         aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCCTGAACCCAACGCTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                <  2:39199/140‑1 (MQ=255)

                                          tttctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                               >  2:564137/3‑140 (MQ=255)

                                          cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTGCACCACGTTCTCTTAATATTTCAg                                                                                               >  2:611476/1‑140 (MQ=255)

                                            tctcATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAGAGTAGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                             <  1:129740/140‑1 (MQ=11)

                                             ctcAACAATACCCCATTTGTAATAAGCTCTTATATGCTATTTCTTCCAAATTTTATATAATATTGGGGTATATTCTTTAACCAAAATGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGata                                                                                            <  1:221682/140‑1 (MQ=11)

                                              tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAAatat                                                                                           >  1:423678/1‑140 (MQ=255)

                                                atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                                                         >  1:171479/1‑140 (MQ=40)

                                                 tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGATCGTTGAATGATGAACGTTTACCCCAAGTTCTCTTAATTTTTCAGatatatg                                                                                        >  1:147891/1‑139 (MQ=11)

                                                 tcaATACGCCATTCGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGACCGTAGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                        <  2:430956/140‑1 (MQ=11)

                                                  caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                       <  1:192247/140‑1 (MQ=21)

                                                  caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCATTAATATTTCAGATATATCa                                                                                       >  2:601144/1‑140 (MQ=19)

                                                    aTACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                                                     <  1:257318/140‑1 (MQ=21)

                                                    aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTCGACCGTTCAATGATGAACGTTTTCACCGCGTTCCATTAATATTTCAGatatacaacg                                                                                     >  2:18589/1‑135 (MQ=14)

                                                    aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATAGAGAAATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGATTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                     >  1:45577/1‑140 (MQ=11)

                                                     tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACGCCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                    >  2:66396/1‑140 (MQ=255)

                                                     tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                    >  1:402853/1‑140 (MQ=255)

                                                       cGCCTTTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                  <  2:19588/140‑1 (MQ=11)

                                                       cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTACAGCTATATCACGATa                                                                                  >  1:249009/1‑140 (MQ=11)

                                                        gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                 <  2:20972/140‑1 (MQ=21)

                                                         ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTATTATTTCATATATATCACGATagc                                                                                >  2:520696/1‑138 (MQ=11)

                                                         ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                <  2:357473/140‑3 (MQ=255)

                                                          cATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAAAAAGATATAAAATTGGGTCATATTCTTGAACCCAACGGAAGATCGTTGAATGACGAACGTTTACACCACGTTCTCTTAAAATTTCAGATATATCACTATAGCt                                                                               >  2:32446/1‑140 (MQ=11)

                                                           aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                              <  2:528791/140‑1 (MQ=11)

                                                           aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGTGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                              <  1:534301/140‑1 (MQ=255)

                                                             ttttaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                            <  1:533084/137‑1 (MQ=21)

                                                              tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGAATAATCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                           >  1:526822/1‑140 (MQ=255)

                                                              tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCCGATATATCACGATAGCTCAAt                                                                           >  1:461802/1‑140 (MQ=11)

                                                              tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                           >  2:131162/1‑140 (MQ=21)

                                                               gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                          <  1:2620/140‑1 (MQ=11)

                                                                taataaGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCCATgc                                                                         >  2:539164/1‑139 (MQ=11)

                                                                taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACGTTAACACCACTGTCTCGTAATATCTCAGATATATCACAATAACGCaaaac                                                                         >  1:467629/1‑137 (MQ=11)

                                                                taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTAAATgc                                                                         >  2:570865/1‑139 (MQ=11)

                                                                 aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGGACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTGAGATATATCACGATAACTCAATgca                                                                        >  1:407767/1‑138 (MQ=255)

                                                                 aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                        <  1:628070/140‑3 (MQ=11)

                                                                 aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                        >  1:512151/1‑138 (MQ=11)

                                                                  ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTAATCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCTTGtat                                                                       >  1:303678/1‑140 (MQ=255)

                                                                     aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGAGCATATTCTTAAACCAAACGGTAGACAGTTGAATGATGAACGTTTACACAACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATc                                                                    >  1:182491/1‑140 (MQ=11)

                                                                              aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                           <  2:105841/140‑1 (MQ=35)

                                                                              aTGCTTCTTCTTCCAAATTTGAGACAAAAGTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAGGTATATCTTAGAtagt                                                           <  1:438547/140‑1 (MQ=11)

                                                                                 cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                        >  2:28385/1‑140 (MQ=34)

                                                                                                           ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                              <  1:551904/140‑1 (MQ=33)

                                                                                                                gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                         >  2:645068/1‑140 (MQ=32)

                                                                                                                 cATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAATGTATATCTTAGATAGTAGCCAACGGCTACAGTAATAAAATACCTGTTAAAt                        >  1:565365/1‑140 (MQ=11)

                                                                                                                                aaCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa         >  2:156500/1‑140 (MQ=32)

                                                                                                                                    gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGt     >  2:275542/1‑140 (MQ=33)

GGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCA  >  CP000730/36276‑36550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`