Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,471 A→G 24.0% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4710AG24.0% 170.1 / 52.5 114S27S (AGT→AGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (45/40);  new base G (14/14);  total (60/54)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTA  >  CP000730/36332‑36610
                                                                                                                                           |                                                                                                                                           
gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATATAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                             <  1:651525/140‑1 (MQ=25)
gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                             <  2:20972/140‑1 (MQ=21)
gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTCGAGCAGATTCTGGAACCTAACGGTACACCGCATAACTAGGAAGGTTGACACCACCTTCCGTTCATCTTTCAGACATATCACGATaa                                                                                                                                             >  2:303162/1‑140 (MQ=2)
 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCGAAAGGAATTCTGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATGTATTACGATAAc                                                                                                                                            >  2:569293/1‑140 (MQ=11)
 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                            <  2:240534/140‑1 (MQ=18)
  cATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAAAAAGATATAAAATTGGGTCATATTCTTGAACCCAACGGAAGATCGTTGAATGACGAACGTTTACACCACGTTCTCTTAAAATTTCAGATATATCACTATAGCt                                                                                                                                           >  2:32446/1‑140 (MQ=11)
   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                          <  1:630100/140‑1 (MQ=11)
   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                          <  2:528791/140‑1 (MQ=11)
   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGTTTTCTTAATATTTCAGATATATCCCGATAGCtt                                                                                                                                          >  2:93292/1‑139 (MQ=11)
   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                          <  2:605295/140‑1 (MQ=18)
   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGTGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                          <  1:534301/140‑1 (MQ=255)
     ttttaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                        <  1:533084/137‑1 (MQ=21)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGAATAATCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                       >  1:526822/1‑140 (MQ=255)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                       >  2:131162/1‑140 (MQ=21)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                       <  1:492450/140‑1 (MQ=18)
      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTCCCCTTAATAGTTCATAGATATAACGATAACTCAAt                                                                                                                                       >  2:293139/1‑140 (MQ=11)
       taaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                      <  2:606692/138‑1 (MQ=255)
       gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCATg                                                                                                                                      >  2:1552/1‑140 (MQ=19)
       gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                      <  1:440489/140‑1 (MQ=40)
        taataaGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCCATgc                                                                                                                                     >  2:539164/1‑139 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAAGTGGGGCATATTCTTGAACCTAACGGTAGACCGTTGAATGATGAACGTCTACACCACGTTCCCTTAATATTTCCGATATATCACGATAACTCAATgc                                                                                                                                     <  2:165868/140‑2 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACGTTAACACCACTGTCTCGTAATATCTCAGATATATCACAATAACGCaaaac                                                                                                                                     >  1:467629/1‑137 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  1:222695/1‑139 (MQ=255)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  2:570834/1‑139 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTAAATgc                                                                                                                                     >  2:570865/1‑139 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                     <  2:451913/140‑2 (MQ=18)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                     <  2:157739/140‑2 (MQ=18)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTGCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  1:465091/1‑139 (MQ=11)
        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGt                                                                                                                                     >  2:252146/1‑140 (MQ=255)
        taataaGCTTTTTTATGCTTATTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  1:402950/1‑139 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:169307/140‑3 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGGTAGCTCAATgca                                                                                                                                    >  2:345742/1‑138 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    >  1:512151/1‑138 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:628070/140‑3 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    >  1:615418/1‑138 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:53867/140‑3 (MQ=11)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                    <  2:619212/140‑3 (MQ=17)
         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAGTTATTCCGGTACCTCAATgca                                                                                                                                    >  1:312750/1‑138 (MQ=11)
         aataaGCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCTGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:424528/140‑3 (MQ=11)
          ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTAATCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCTTGtat                                                                                                                                   >  1:303678/1‑140 (MQ=255)
            aaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATCGCTCAATgactat                                                                                                                                 >  2:664917/1‑135 (MQ=11)
             aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATACATCACGATAACTCAATGTATATc                                                                                                                                <  1:628771/140‑1 (MQ=38)
             aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGAGCATATTCTTAAACCAAACGGTAGACAGTTGAATGATGAACGTTTACACAACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATc                                                                                                                                >  1:182491/1‑140 (MQ=11)
               cTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                                              <  2:476748/140‑1 (MQ=37)
                 tttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                                                                                                                            >  2:51894/1‑140 (MQ=34)
                    ttATGCTTTTTCTTCCAAATTTGATCCAAAATTGGGGCATATTCTTTAACCCAACGGTAAAACGTTGAATGATGAAAGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAACTTAGAta                                                                                                                         >  1:217367/1‑140 (MQ=11)
                    ttATGCTTTTTCTTCCAAATTTGATACATAATTGGGGCATATTCTTGTTCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                                                                                                                         <  1:184666/140‑1 (MQ=255)
                     tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                                                        >  1:389170/1‑140 (MQ=34)
                      aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                       <  1:163321/140‑1 (MQ=35)
                      aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                       <  1:68217/140‑1 (MQ=35)
                      aTGCTTCTTCTTCCAAATTTGAGACAAAAGTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAGGTATATCTTAGAtagt                                                                                                                       <  1:438547/140‑1 (MQ=11)
                         cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTCATATTTCAGATATATCACGATAACCCACTGTCTATCTTAGAGAGTAGc                                                                                                                    >  2:519474/1‑140 (MQ=11)
                         cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                                                    >  2:28385/1‑140 (MQ=34)
                                ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                                             <  2:609109/140‑1 (MQ=37)
                                    aaaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAAATAGTAGCCAACGGCTACa                                                                                                         >  2:521121/1‑140 (MQ=25)
                                         tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAGATATATCACGATAACTCAATGTAAATCTTAGATAGTAGCCAACGGCTACAGTGag                                                                                                    >  1:311526/1‑139 (MQ=255)
                                             aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACa                                                                                                <  2:524853/140‑1 (MQ=34)
                                              cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAt                                                                                               >  2:118336/1‑140 (MQ=33)
                                                aaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                                                                             >  1:14435/1‑140 (MQ=32)
                                                aaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAAACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                                                                             >  1:14716/1‑140 (MQ=17)
                                                aaaTTGGGGCAAATTCTTGAACTCAACGTGAAACCGATGAATGATGAACGTTTACACCACGTTCGCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGACTACAGTGATCACCTcc                                                                                             >  2:659706/1‑140 (MQ=11)
                                                   ttGGGGCATATTCTTGAACCCATCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                          <  2:630548/140‑1 (MQ=18)
                                                   ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                          <  1:551904/140‑1 (MQ=33)
                                                   ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                          <  1:415589/140‑1 (MQ=33)
                                                    tGGGGCATATTCTTGAACCCAACGGTAGACGGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTTAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                         <  1:40507/140‑1 (MQ=16)
                                                     ggggCATCTTGGTGAACCCAACGGTAGACCGTTGCACTGGGACCGTTTAAACGCCCACCTATAAGTATATCAAATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACGGTGATAACATCCTTGtt                                                                                        >  2:140449/1‑140 (MQ=2)
                                                      gggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACGCAATGTAGATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTa                                                                                       <  2:283526/140‑1 (MQ=17)
                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                      >  1:109408/1‑140 (MQ=32)
                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                      >  1:552538/1‑140 (MQ=33)
                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATACTTGTTaa                                                                                      >  1:73872/1‑140 (MQ=18)
                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACACAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                      >  1:207991/1‑140 (MQ=17)
                                                        gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACTTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTTAATCTTGAATAGTAGCCAACGGCTACAGTGGTAAAATCCTTTTTaaa                                                                                     >  2:86028/1‑140 (MQ=11)
                                                         cATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAATGTATATCTTAGATAGTAGCCAACGGCTACAGTAATAAAATACCTGTTAAAt                                                                                    >  1:565365/1‑140 (MQ=11)
                                                          atatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAtt                                                                                   >  2:404552/1‑140 (MQ=33)
                                                            atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                                                 <  1:252146/140‑1 (MQ=32)
                                                                    aCCCAACGGTAGACCGTTGAATGACGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                         <  2:204354/140‑1 (MQ=17)
                                                                        aaCGCTAGTCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                     <  2:128669/140‑1 (MQ=18)
                                                                           ggTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                                                  >  1:397917/1‑140 (MQ=32)
                                                                            gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGt                                                                 >  2:275542/1‑140 (MQ=33)
                                                                              ctaCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTc                                                               >  1:413571/3‑140 (MQ=17)
                                                                                   gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAAATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATATGAAATAGTACATAcc                                                          >  1:84679/1‑139 (MQ=12)
                                                                                      gAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaa                                                       <  1:608582/140‑1 (MQ=33)
                                                                                       aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                      >  1:536092/1‑140 (MQ=32)
                                                                                       aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                      >  1:535945/1‑140 (MQ=32)
                                                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCCACGGCTACAGTGATAACAACCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                    >  1:284007/1‑140 (MQ=17)
                                                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTCTATCTGAAATAGTTCATACAGAAGAc                                                    <  2:413571/140‑1 (MQ=17)
                                                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                    <  1:275542/140‑1 (MQ=33)
                                                                                            tgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTcc                                                 <  1:118336/140‑1 (MQ=32)
                                                                                                    tACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                         <  2:246545/140‑1 (MQ=33)
                                                                                                     acacCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACTAGAGACGCCTTTTTGTTa                                        >  1:582957/1‑140 (MQ=255)
                                                                                                         cacGTTGTCTTAATATTTCAGATATATCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt                                    <  1:261050/140‑1 (MQ=12)
                                                                                                                    aaTATTTCATATATATCAAGATAATGCAATGTATATCTTAGATTGTAGTTAATGTCTTCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                         <  1:18589/140‑1 (MQ=11)
                                                                                                                    aaTATTTCAGAGATATCATGATAACTCAATGTATATCTTAGATAGTAGTCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                         <  1:121266/140‑1 (MQ=255)
                                                                                                                     atatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                        <  2:562477/140‑1 (MQ=34)
                                                                                                                              atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg               <  2:171479/140‑1 (MQ=35)
                                                                                                                                atatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa             <  2:45790/140‑1 (MQ=35)
                                                                                                                                  atCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc           <  1:96730/140‑1 (MQ=21)
                                                                                                                                       gcaaaCACAATGCATATCTTAGAAAGTAGCCAAACGCTACAGCGATACCATCCTTGTTAAATTCTTTATATCTGCAATAGTTCATACAGATGACTCCTTTATGTTAAAATTATACTATTAATTCAACTTTGCAACAgccc      <  2:297821/137‑4 (MQ=255)
                                                                                                                                           aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGACCCGta  >  2:345241/1‑140 (MQ=38)
                                                                                                                                           aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta  >  2:335032/1‑140 (MQ=39)
                                                                                                                                           |                                                                                                                                           
GCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTA  >  CP000730/36332‑36610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: