BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,471	A→G	24.0%	S27S (AGT→AGC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,471	0	A	G	24.0%	170.1 / 52.5	114	S27S (AGT→AGC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base A (45/40); new base G (14/14); total (60/54)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTA  >  CP000730/36332‑36610

gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATATAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                             <  1:651525/140‑1 (MQ=25)

gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                             <  2:20972/140‑1 (MQ=21)

gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTCGAGCAGATTCTGGAACCTAACGGTACACCGCATAACTAGGAAGGTTGACACCACCTTCCGTTCATCTTTCAGACATATCACGATaa                                                                                                                                             >  2:303162/1‑140 (MQ=2)

 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCGAAAGGAATTCTGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATGTATTACGATAAc                                                                                                                                            >  2:569293/1‑140 (MQ=11)

 ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                            <  2:240534/140‑1 (MQ=18)

  cATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAAAAAGATATAAAATTGGGTCATATTCTTGAACCCAACGGAAGATCGTTGAATGACGAACGTTTACACCACGTTCTCTTAAAATTTCAGATATATCACTATAGCt                                                                                                                                           >  2:32446/1‑140 (MQ=11)

   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                          <  1:630100/140‑1 (MQ=11)

   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                          <  2:528791/140‑1 (MQ=11)

   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAAACCACGTTTTCTTAATATTTCAGATATATCCCGATAGCtt                                                                                                                                          >  2:93292/1‑139 (MQ=11)

   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                          <  2:605295/140‑1 (MQ=18)

   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGTGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                          <  1:534301/140‑1 (MQ=255)

     ttttaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                        <  1:533084/137‑1 (MQ=21)

      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGAATAATCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                       >  1:526822/1‑140 (MQ=255)

      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                       >  2:131162/1‑140 (MQ=21)

      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                       <  1:492450/140‑1 (MQ=18)

      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTCCCCTTAATAGTTCATAGATATAACGATAACTCAAt                                                                                                                                       >  2:293139/1‑140 (MQ=11)

       taaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                      <  2:606692/138‑1 (MQ=255)

       gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCATg                                                                                                                                      >  2:1552/1‑140 (MQ=19)

       gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                      <  1:440489/140‑1 (MQ=40)

        taataaGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCCATgc                                                                                                                                     >  2:539164/1‑139 (MQ=11)

        taataaGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAAGTGGGGCATATTCTTGAACCTAACGGTAGACCGTTGAATGATGAACGTCTACACCACGTTCCCTTAATATTTCCGATATATCACGATAACTCAATgc                                                                                                                                     <  2:165868/140‑2 (MQ=11)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACGTTAACACCACTGTCTCGTAATATCTCAGATATATCACAATAACGCaaaac                                                                                                                                     >  1:467629/1‑137 (MQ=11)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  1:222695/1‑139 (MQ=255)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  2:570834/1‑139 (MQ=11)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTAAATgc                                                                                                                                     >  2:570865/1‑139 (MQ=11)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                     <  2:451913/140‑2 (MQ=18)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                     <  2:157739/140‑2 (MQ=18)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTGCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  1:465091/1‑139 (MQ=11)

        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGt                                                                                                                                     >  2:252146/1‑140 (MQ=255)

        taataaGCTTTTTTATGCTTATTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                     >  1:402950/1‑139 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:169307/140‑3 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGGTAGCTCAATgca                                                                                                                                    >  2:345742/1‑138 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    >  1:512151/1‑138 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:628070/140‑3 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    >  1:615418/1‑138 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:53867/140‑3 (MQ=11)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                    <  2:619212/140‑3 (MQ=17)

         aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAGTTATTCCGGTACCTCAATgca                                                                                                                                    >  1:312750/1‑138 (MQ=11)

         aataaGCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCTGATATATCACGATAGCTCAATgca                                                                                                                                    <  1:424528/140‑3 (MQ=11)

          ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTAATCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCCTTGtat                                                                                                                                   >  1:303678/1‑140 (MQ=255)

            aaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATCGCTCAATgactat                                                                                                                                 >  2:664917/1‑135 (MQ=11)

             aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATACATCACGATAACTCAATGTATATc                                                                                                                                <  1:628771/140‑1 (MQ=38)

             aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGAGCATATTCTTAAACCAAACGGTAGACAGTTGAATGATGAACGTTTACACAACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATc                                                                                                                                >  1:182491/1‑140 (MQ=11)

               cTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                                              <  2:476748/140‑1 (MQ=37)

                 tttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                                                                                                                            >  2:51894/1‑140 (MQ=34)

                    ttATGCTTTTTCTTCCAAATTTGATCCAAAATTGGGGCATATTCTTTAACCCAACGGTAAAACGTTGAATGATGAAAGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATAACTTAGAta                                                                                                                         >  1:217367/1‑140 (MQ=11)

                    ttATGCTTTTTCTTCCAAATTTGATACATAATTGGGGCATATTCTTGTTCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                                                                                                                         <  1:184666/140‑1 (MQ=255)

                     tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                                                                                                        >  1:389170/1‑140 (MQ=34)

                      aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                       <  1:163321/140‑1 (MQ=35)

                      aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                       <  1:68217/140‑1 (MQ=35)

                      aTGCTTCTTCTTCCAAATTTGAGACAAAAGTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAGGTATATCTTAGAtagt                                                                                                                       <  1:438547/140‑1 (MQ=11)

                         cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTCATATTTCAGATATATCACGATAACCCACTGTCTATCTTAGAGAGTAGc                                                                                                                    >  2:519474/1‑140 (MQ=11)

                         cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                                                    >  2:28385/1‑140 (MQ=34)

                                ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                                             <  2:609109/140‑1 (MQ=37)

                                    aaaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAAATAGTAGCCAACGGCTACa                                                                                                         >  2:521121/1‑140 (MQ=25)

                                         tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAGATATATCACGATAACTCAATGTAAATCTTAGATAGTAGCCAACGGCTACAGTGag                                                                                                    >  1:311526/1‑139 (MQ=255)

                                             aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACa                                                                                                <  2:524853/140‑1 (MQ=34)

                                              cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAt                                                                                               >  2:118336/1‑140 (MQ=33)

                                                aaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                                                                             >  1:14435/1‑140 (MQ=32)

                                                aaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAAACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                                                                             >  1:14716/1‑140 (MQ=17)

                                                aaaTTGGGGCAAATTCTTGAACTCAACGTGAAACCGATGAATGATGAACGTTTACACCACGTTCGCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGACTACAGTGATCACCTcc                                                                                             >  2:659706/1‑140 (MQ=11)

                                                   ttGGGGCATATTCTTGAACCCATCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                          <  2:630548/140‑1 (MQ=18)

                                                   ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                          <  1:551904/140‑1 (MQ=33)

                                                   ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                          <  1:415589/140‑1 (MQ=33)

                                                    tGGGGCATATTCTTGAACCCAACGGTAGACGGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTTAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                         <  1:40507/140‑1 (MQ=16)

                                                     ggggCATCTTGGTGAACCCAACGGTAGACCGTTGCACTGGGACCGTTTAAACGCCCACCTATAAGTATATCAAATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACGGTGATAACATCCTTGtt                                                                                        >  2:140449/1‑140 (MQ=2)

                                                      gggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACGCAATGTAGATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTa                                                                                       <  2:283526/140‑1 (MQ=17)

                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                      >  1:109408/1‑140 (MQ=32)

                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                      >  1:552538/1‑140 (MQ=33)

                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATACTTGTTaa                                                                                      >  1:73872/1‑140 (MQ=18)

                                                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACACAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                      >  1:207991/1‑140 (MQ=17)

                                                        gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACTTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTTAATCTTGAATAGTAGCCAACGGCTACAGTGGTAAAATCCTTTTTaaa                                                                                     >  2:86028/1‑140 (MQ=11)

                                                         cATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAATGTATATCTTAGATAGTAGCCAACGGCTACAGTAATAAAATACCTGTTAAAt                                                                                    >  1:565365/1‑140 (MQ=11)

                                                          atatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAtt                                                                                   >  2:404552/1‑140 (MQ=33)

                                                            atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                                                 <  1:252146/140‑1 (MQ=32)

                                                                    aCCCAACGGTAGACCGTTGAATGACGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                         <  2:204354/140‑1 (MQ=17)

                                                                        aaCGCTAGTCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                     <  2:128669/140‑1 (MQ=18)

                                                                           ggTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                                                  >  1:397917/1‑140 (MQ=32)

                                                                            gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGt                                                                 >  2:275542/1‑140 (MQ=33)

                                                                              ctaCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTc                                                               >  1:413571/3‑140 (MQ=17)

                                                                                   gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAAATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATATGAAATAGTACATAcc                                                          >  1:84679/1‑139 (MQ=12)

                                                                                      gAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaa                                                       <  1:608582/140‑1 (MQ=33)

                                                                                       aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                      >  1:536092/1‑140 (MQ=32)

                                                                                       aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                      >  1:535945/1‑140 (MQ=32)

                                                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCCACGGCTACAGTGATAACAACCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                    >  1:284007/1‑140 (MQ=17)

                                                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTCTATCTGAAATAGTTCATACAGAAGAc                                                    <  2:413571/140‑1 (MQ=17)

                                                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                    <  1:275542/140‑1 (MQ=33)

                                                                                            tgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTcc                                                 <  1:118336/140‑1 (MQ=32)

                                                                                                    tACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                         <  2:246545/140‑1 (MQ=33)

                                                                                                     acacCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACTAGAGACGCCTTTTTGTTa                                        >  1:582957/1‑140 (MQ=255)

                                                                                                         cacGTTGTCTTAATATTTCAGATATATCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt                                    <  1:261050/140‑1 (MQ=12)

                                                                                                                    aaTATTTCATATATATCAAGATAATGCAATGTATATCTTAGATTGTAGTTAATGTCTTCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                         <  1:18589/140‑1 (MQ=11)

                                                                                                                    aaTATTTCAGAGATATCATGATAACTCAATGTATATCTTAGATAGTAGTCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                         <  1:121266/140‑1 (MQ=255)

                                                                                                                     atatTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                        <  2:562477/140‑1 (MQ=34)

                                                                                                                              atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg               <  2:171479/140‑1 (MQ=35)

                                                                                                                                atatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa             <  2:45790/140‑1 (MQ=35)

                                                                                                                                  atCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc           <  1:96730/140‑1 (MQ=21)

                                                                                                                                       gcaaaCACAATGCATATCTTAGAAAGTAGCCAAACGCTACAGCGATACCATCCTTGTTAAATTCTTTATATCTGCAATAGTTCATACAGATGACTCCTTTATGTTAAAATTATACTATTAATTCAACTTTGCAACAgccc      <  2:297821/137‑4 (MQ=255)

                                                                                                                                           aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGACCCGta  >  2:345241/1‑140 (MQ=38)

                                                                                                                                           aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta  >  2:335032/1‑140 (MQ=39)

GCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTA  >  CP000730/36332‑36610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`