BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,501	G→A	6.9%	A17A (GCC→GCT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,501	0	G	A	6.9%	255.4 / 8.0	116	A17A (GCC→GCT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (56/50); new base A (4/4); total (60/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 116 total aligned reads displayed.

TTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTA  >  CP000730/36364‑36639

ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGc                                                                                                                                          <  2:609109/140‑1 (MQ=37)

    aaaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAAATAGTAGCCAACGGCTACa                                                                                                                                      >  2:521121/1‑140 (MQ=25)

         tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑AAATCTTAGATAGTAGCCAACGGCTACAGTGag                                                                                                                                 >  1:311526/1‑139 (MQ=255)

              cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAt                                                                                                                            >  2:118336/1‑140 (MQ=33)

                aaaTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATcc                                                                                                                          >  1:14435/1‑140 (MQ=32)

                aaaTTGGGGCAAATTCTTGAACTCAACGTGAAACCGATGAATGATGAACGTTTACACCACGTTCGCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGACTACAGTGATCACCTcc                                                                                                                          >  2:659706/1‑140 (MQ=11)

                   ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                                                       <  1:551904/140‑1 (MQ=33)

                    tGGGGCATATTCTTGAACCCAACGGTAGACGGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTTAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                                                      <  1:40507/140‑1 (MQ=16)

                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                                                   >  1:109408/1‑140 (MQ=32)

                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATACTTGTTaa                                                                                                                   >  1:73872/1‑140 (MQ=18)

                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACACAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                                                   >  1:207991/1‑140 (MQ=17)

                       ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCATTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATGCTTGTTaa                                                                                                                   >  1:37007/1‑140 (MQ=17)

                        gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACTTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑TAATCTTGAATAGTAGCCAACGGCTACAGTGGTAAAATCCTTTTTaaa                                                                                                                  >  2:86028/1‑140 (MQ=11)

                        gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                                                                                  >  2:645068/1‑140 (MQ=32)

                         cATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTAATAAAATACCTGTTAAAt                                                                                                                 >  1:565365/1‑140 (MQ=11)

                          atatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAtt                                                                                                                >  2:404552/1‑140 (MQ=33)

                            atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                                                                              <  1:252146/140‑1 (MQ=32)

                                    aCCCAACGGTAGACCGTTGAATGACGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                                                      <  2:204354/140‑1 (MQ=17)

                                        aaCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                                                  >  2:156500/1‑140 (MQ=32)

                                        aaCGCTAGTCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                                                  <  2:128669/140‑1 (MQ=18)

                                           ggTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg                                                                                               >  1:397917/1‑140 (MQ=32)

                                            gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGt                                                                                              >  2:275542/1‑140 (MQ=33)

                                              ctaCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTc                                                                                            >  1:413571/3‑140 (MQ=17)

                                               gACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCa                                                                                           >  1:530069/1‑140 (MQ=32)

                                                   gTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAAATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATATGAAATAGTACATAcc                                                                                       >  1:84679/1‑139 (MQ=12)

                                                      gAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaa                                                                                    <  1:608582/140‑1 (MQ=33)

                                                       aaTGATTAATGTTTACACCATGTTTTCTTAATATTTCAAATATATCACGATAGCTCAATGT‑GTATTTTAGTTAGTAGCCAACGGCTACAGTGATAACAGCCTTGCTCTCTTTATAACATCGGAAATAGTTCATACagaag                                                                                   <  1:159411/140‑1 (MQ=2)

                                                       aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                                                   >  1:536092/1‑140 (MQ=32)

                                                       aaTGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                                                   >  1:535945/1‑140 (MQ=32)

                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCCACGGCTACAGTGATAACAACCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                                                 >  1:284007/1‑140 (MQ=17)

                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTCTATCTGAAATAGTTCATACAGAAGAc                                                                                 <  2:413571/140‑1 (MQ=17)

                                                         tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGAc                                                                                 <  1:275542/140‑1 (MQ=33)

                                                            tgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTcc                                                                              <  1:118336/140‑1 (MQ=32)

                                                                    tACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                                                      <  2:246545/140‑1 (MQ=33)

                                                                     acacCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACTAGAGACGCCTTTTTGTTa                                                                     >  1:582957/1‑140 (MQ=255)

                                                                     acacCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATAGCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTa                                                                     >  2:491271/1‑140 (MQ=17)

                                                                           ctttCTCTTAATTATTCAGTGATAGCACGATAGCTC‑ATGTCATATCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATta                                                               <  2:582835/138‑1 (MQ=11)

                                                                           cGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATta                                                               <  2:534056/140‑1 (MQ=33)

                                                                                    aaTATTTCATATATATCAAGATAATGCAATGT‑ATATCTTAGATTGTAGTTAATGTCTTCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                      <  1:18589/140‑1 (MQ=11)

                                                                                    aaTATTTCAGAGATATCATGATAACTCAATGT‑ATATCTTAGATAGTAGTCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                      <  1:121266/140‑1 (MQ=255)

                                                                                     atatTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                                                     <  2:562477/140‑1 (MQ=34)

                                                                                              atatatCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg                                            <  2:171479/140‑1 (MQ=35)

                                                                                                atatCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                          <  2:45790/140‑1 (MQ=35)

                                                                                                  atCACGATAGCTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAc                                        <  1:96730/140‑1 (MQ=21)

                                                                                                     aCGATAGCTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCCTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGa                                     <  2:94054/140‑1 (MQ=255)

                                                                                                       gcaaaCACAATGC‑ATATCTTAGAAAGTAGCCAAACGCTACAGCGATACCATCCTTGTTAAATTCTTTATATCTGCAATAGTTCATACAGATGACTCCTTTATGTTAAAATTATACTATTAATTCAACTTTGCAACAgccc                                   <  2:297821/137‑4 (MQ=255)

                                                                                                        taaaCTCTATGT‑ATATCTGAGTTATTAGCCGACGTCGACCGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTCTAAATTCCACTTTGCAACAGAAcc                                  <  2:313885/138‑1 (MQ=11)

                                                                                                          agcTCAATGC‑ATATCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGATATAGTTCATACAGAAGTTTCCTTTTTGTTAAGATTATATTATAAATTCAACTTTGCAACAGAACCGt                                <  1:510464/138‑1 (MQ=11)

                                                                                                          agcTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTATTTTTTTATTATAAAATCAGCTTTCCAACACAACCGt                                >  2:250113/3‑140 (MQ=11)

                                                                                                          agcTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTAGTTAAATAGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                >  1:201148/3‑140 (MQ=11)

                                                                                                           gcTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGAAACAGAACCGta                               >  1:176325/2‑140 (MQ=12)

                                                                                                           aCTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGACCCGta                               >  2:345241/1‑140 (MQ=38)

                                                                                                           aCTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta                               >  2:335032/1‑140 (MQ=39)

                                                                                                            cTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTTAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                              >  2:133445/1‑140 (MQ=255)

                                                                                                            cTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTTCAACAGAACCGtat                              >  2:409100/1‑140 (MQ=255)

                                                                                                            cTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                              >  1:165123/1‑140 (MQ=12)

                                                                                                            cTCAATGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                              >  2:407495/1‑140 (MQ=16)

                                                                                                              tcaagtcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctccta                             <  2:278809/133‑6 (MQ=11)

                                                                                                              cAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                            >  1:217589/1‑140 (MQ=255)

                                                                                                               aaTGG‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAAGATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAATAGAACCGtatttt                           >  1:476929/1‑138 (MQ=11)

                                                                                                               aaTGC‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                           <  1:625514/140‑3 (MQ=255)

                                                                                                                 atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTCTGCAACAGAACCGtatttta                          <  1:403360/136‑4 (MQ=11)

                                                                                                                  tggatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAATCTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGGAACAGAAcctcctaactg                         <  1:523037/137‑10 (MQ=11)

                                                                                                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTTTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                        <  1:266827/138‑6 (MQ=11)

                                                                                                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                        <  2:662111/138‑6 (MQ=255)

                                                                                                                   gcatatCTTAGATAGTAGCCAACAGCTACAGTGATAACACCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttacta                        >  1:223220/3‑130 (MQ=1)

                                                                                                                   gcatatCTGTGATAGTAGCCAACGGCTACAGTGATAACATCCTTTTTAAATTGTTTATATCTGAACTAGTTCATAGAGAAGGCACGTTTTGGTAAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                        <  2:61228/138‑6 (MQ=11)

                                                                                                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATTCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                       >  2:376878/2‑129 (MQ=11)

                                                                                                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                       <  1:248386/139‑7 (MQ=255)

                                                                                                                     atttCTTAGATAGTAGCCAACGGCTACAGTGATAACCTCCTTTTTCAATGTCTTATATCTGAAATAGTTCATACAGTAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCATCAGATCCGtattttatgag                      <  2:147891/137‑8 (MQ=11)

                                                                                                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctactaactgtat                      >  2:256609/1‑128 (MQ=11)

                                                                                                                     atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactata                      >  1:547735/1‑128 (MQ=11)

                                                                                                                     atatCTTACATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAAACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                      <  2:147980/140‑8 (MQ=11)

                                                                                                                     atatCTGAGAAAGTAGCCAACGGCTACAGTGATCACATCCAAGACACGTTCTATATCTCTGAAATAGTTAATACAGATGGCTCCATTTTGTTAAAATTATATTATGAATTAAACTTTACACCAGAccctcctagctgtat                      >  2:79602/1‑128 (MQ=2)

                                                                                                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                     <  1:464694/140‑1 (MQ=255)

                                                                                                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACGTTGCAACAGAACCGTATTATGGAATa                     <  1:51894/140‑1 (MQ=255)

                                                                                                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGTTTTCTTTTTGTTAAAATTATACTATAAATGCAACTTTGCAACAGAGcctcatttactatat                     <  1:63110/140‑14 (MQ=11)

                                                                                                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                     <  1:219227/140‑14 (MQ=11)

                                                                                                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                     <  1:358985/140‑14 (MQ=11)

                                                                                                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAAGATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAACGtattttatgagta                    >  1:140938/1‑131 (MQ=11)

                                                                                                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    >  1:115313/1‑126 (MQ=11)

                                                                                                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                    >  1:644896/1‑131 (MQ=255)

                                                                                                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTAAAAATTCAACTTTGCAACAGAACCGtattttattagta                    >  1:146566/1‑131 (MQ=11)

                                                                                                                       atCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAAcctcttttactatatg                    >  2:36905/1‑126 (MQ=11)

                                                                                                                       atCTTAGATAGTAGCCAACAGCTACAGTCATAACATCCTTGTTAAATAGTTTATCTCTGAAATAATTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTATGCAACAGAAcctcatttactatatg                    >  1:430172/1‑126 (MQ=11)

                                                                                                                       atCTGAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                    <  2:513869/140‑15 (MQ=11)

                                                                                                                        tCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTCATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAGTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   <  2:377702/140‑11 (MQ=11)

                                                                                                                        tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                   >  2:475420/1‑130 (MQ=11)

                                                                                                                         cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca                  <  2:274664/140‑12 (MQ=11)

                                                                                                                          ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacac                 <  1:478917/140‑13 (MQ=11)

                                                                                                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                <  1:287331/140‑14 (MQ=11)

                                                                                                                           tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                >  1:173918/1‑140 (MQ=255)

                                                                                                                            aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc               <  1:262311/140‑15 (MQ=11)

                                                                                                                            aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc               >  2:393736/1‑126 (MQ=11)

                                                                                                                            aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttataaaaacacac               <  2:60426/140‑15 (MQ=11)

                                                                                                                                agtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgg           >  2:478246/1‑140 (MQ=255)

                                                                                                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACTGTATTACGGAATAGAGATATTGATaa        >  2:135387/1‑140 (MQ=255)

                                                                                                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa        >  1:367217/1‑140 (MQ=255)

                                                                                                                                   agCCAACGGCTACAGGGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa        <  2:311526/140‑1 (MQ=255)

                                                                                                                                         cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtttt  >  2:181122/1‑139 (MQ=255)

TTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGT‑ATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTA  >  CP000730/36364‑36639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`