BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,872	G→A	53.1%	intergenic (‑955/‑213)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,872	0	G	A	53.1%	9.8 / 212.9	164	intergenic (‑955/‑213)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (32/44); new base A (49/38); total (81/83)
Fisher's exact test for biased strand distribution p-value = 8.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 165 total aligned reads displayed.

CGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATA  >  CP000730/1929733‑1930008

cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑g                                                                                                                                          <  1:107495/140‑1 (MQ=255)

   ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCtt                                                                                                                                       >  2:116665/1‑140 (MQ=255)

   ttCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCACCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCtt                                                                                                                                       >  2:180334/1‑140 (MQ=255)

     cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTAt                                                                                                                                     <  1:416060/140‑1 (MQ=255)

      aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCGTA‑TAC‑G‑G‑TGAT‑GCTTAtt                                                                                                                                    >  2:420496/1‑140 (MQ=255)

       aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTAttt                                                                                                                                   >  2:127954/1‑140 (MQ=255)

        tCTATCAGCTTGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTAtttt                                                                                                                                  <  1:239395/140‑1 (MQ=255)

          tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCg                                                                                                                                <  1:327701/140‑1 (MQ=255)

          tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCg                                                                                                                                <  1:327669/140‑1 (MQ=255)

                cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTcaca                                                                                                                          <  1:195487/140‑1 (MQ=255)

                        atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCATATTTTCGTTCACATCATAGcc                                                                                                                  >  2:601680/1‑139 (MQ=255)

                            aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAg                                                                                                              <  2:232125/140‑1 (MQ=255)

                                   cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                                                                                       >  1:642826/1‑140 (MQ=255)

                                     atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAATCAACAGTgg                                                                                                    >  2:194350/15‑140 (MQ=255)

                                      tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                   >  1:80227/14‑140 (MQ=255)

                                        ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                 <  2:564867/129‑1 (MQ=255)

                                        ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                 >  1:609342/12‑140 (MQ=255)

                                         gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGTGTTGACTAACTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                <  1:314332/130‑1 (MQ=255)

                                          aagtagatatggttGTATATTCACAGATGGTATAATAACAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                               <  2:392132/128‑1 (MQ=255)

                                           agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                              <  1:512835/132‑1 (MQ=255)

                                           aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                               <  1:519469/140‑1 (MQ=255)

                                           aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCggtg                                                                                               >  2:343325/1‑140 (MQ=255)

                                            gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCCCCCCAAACCACCAGTCATCAGTGGCTGTGct                                                                                             <  1:490424/133‑2 (MQ=255)

                                            gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                             >  1:97691/8‑139 (MQ=255)

                                              agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GTCGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                           <  1:230962/135‑4 (MQ=255)

                                              agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                           <  1:118944/135‑4 (MQ=255)

                                              agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                           <  2:25474/135‑4 (MQ=255)

                                               gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtgatgtt                                                                                          >  2:484475/5‑135 (MQ=255)

                                               gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                          <  1:246185/136‑5 (MQ=255)

                                               gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                          >  2:103259/5‑136 (MQ=255)

                                                cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                          >  1:407211/1‑140 (MQ=255)

                                                 tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑TTA‑ACGAAGAAAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtttc                                                                                        >  1:554713/3‑134 (MQ=255)

                                                 tatgCTTGTATATTCACAGAGGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                                        <  2:267487/138‑7 (MQ=255)

                                                  gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTTTCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                        <  1:123323/140‑1 (MQ=255)

                                                      ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                   <  2:340199/140‑4 (MQ=255)

                                                      ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                   <  1:500520/140‑4 (MQ=255)

                                                       tGTATATTCACAAATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                                  <  1:59706/140‑5 (MQ=255)

                                                        gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                 <  1:652921/140‑3 (MQ=255)

                                                         tataTTTGCCGATGAAATAATAGGAGAGTTGCCTATCTCTCAGGCGTCAATTTAGTCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCCATTGCGtttttt                                                                                 <  1:261145/140‑1 (MQ=255)

                                                             ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                            >  1:378478/7‑140 (MQ=255)

                                                              tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                           >  2:223806/6‑140 (MQ=255)

                                                               cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGATGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                          >  1:297137/5‑140 (MQ=255)

                                                               cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                          >  2:250494/5‑140 (MQ=255)

                                                                acagatagtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                         <  2:152124/132‑1 (MQ=255)

                                                                 cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAAAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGGTAACAACATAGGACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTgg                                                                        >  1:337951/3‑140 (MQ=255)

                                                                   gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                       <  1:437332/140‑1 (MQ=255)

                                                                   gatgatATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                       <  1:110705/140‑1 (MQ=255)

                                                                     tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCtg                                                                     <  2:576950/140‑2 (MQ=255)

                                                                       ttataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                  <  2:615958/139‑1 (MQ=255)

                                                                       gttaaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                  <  2:332591/136‑1 (MQ=255)

                                                                       atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                   <  2:495638/140‑1 (MQ=255)

                                                                       atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                   <  2:658037/140‑1 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                 >  2:558378/1‑140 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAA‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCATTCATCAGTGGCTTTTCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                 >  2:157312/1‑140 (MQ=255)

                                                                         ttaataGTAGAGCCGCTGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                 >  2:146383/2‑140 (MQ=255)

                                                                          taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATAATAGCACCAGTTATCAGTGGCTGTGCCAATGCATTTTTTTCTTATTGGCTAAGTAg                                                                <  1:28062/140‑1 (MQ=255)

                                                                           aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                              <  1:540592/140‑4 (MQ=255)

                                                                           aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                              >  1:667840/1‑140 (MQ=255)

                                                                            ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCT‑TTA‑G‑T‑TCTTAAAGCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                             <  1:154182/140‑1 (MQ=255)

                                                                            ataCTAGAGTAGCCTATCTCTCAGGCAAAAATT‑‑GAAGAAGAGAGGAGGTGAATTAA‑T‑T‑TGAA‑AAGCATTTTCAGTTGCATCAGATCACCAGTCATCAGTGGCTGTGGTGTTGCGTATGATACTTATTGGCTTAGTAAACg                                                             <  2:657606/140‑1 (MQ=255)

                                                                               atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGACGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                          >  2:433154/2‑140 (MQ=255)

                                                                                   agacGCCTATCTCTCAGGCGACAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                      <  1:179096/137‑5 (MQ=255)

                                                                                   agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCATTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                       >  2:371126/1‑140 (MQ=255)

                                                                                   agTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                       <  1:173036/140‑1 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                     >  1:631400/1‑135 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGATCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATCCTTATTGGCTTAGTAAACGCaaaaaat                                                     >  2:188322/1‑135 (MQ=255)

                                                                                     tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                     >  1:284975/1‑140 (MQ=255)

                                                                                     tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑TCTCAATTGCCGCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                    <  1:484475/140‑7 (MQ=255)

                                                                                     tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                    >  2:540239/1‑134 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACTCAATACaaaa                                                   >  1:467819/1‑140 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                                   <  2:177297/140‑1 (MQ=255)

                                                                                        ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATACAAAAt                                                  >  1:264990/1‑140 (MQ=255)

                                                                                        ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                 >  1:53523/1‑131 (MQ=255)

                                                                                        ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                 >  1:30862/1‑131 (MQ=255)

                                                                                         cTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCCGTCGATGTGCTGTTGCGTATTATACGTATTGGCTTAGTAAACGCaacaaataatta                                                <  2:304254/140‑11 (MQ=255)

                                                                                          tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTCC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTAGCTTAGTAAACACaacaagtaattta                                               >  1:649359/1‑129 (MQ=255)

                                                                                          tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGATGTTGCGTATAATACTTATTGGCTTGGTAAACGCaacaaataattaa                                               >  1:451416/1‑129 (MQ=255)

                                                                                           aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                              >  1:314945/1‑128 (MQ=255)

                                                                                             ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                            <  2:660952/140‑15 (MQ=255)

                                                                                             ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                            >  2:607649/1‑126 (MQ=255)

                                                                                                  aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                        >  2:53056/1‑140 (MQ=255)

                                                                                                       tccaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGcc                                   <  2:654316/137‑1 (MQ=255)

                                                                                                         aaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGc                                 >  2:298503/1‑140 (MQ=255)

                                                                                                          aattgacgcctgagagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                <  1:45457/129‑1 (MQ=255)

                                                                                                          aTTTAGGCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                <  2:117695/140‑1 (MQ=255)

                                                                                                            ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTTACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                              >  2:355254/1‑140 (MQ=255)

                                                                                                                     gagagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGAACCAATAAAAAt                     >  1:373733/1‑140 (MQ=255)

                                                                                                                      agagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATc                    >  1:442154/1‑140 (MQ=255)

                                                                                                                      agagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATc                    <  1:581282/140‑1 (MQ=255)

                                                                                                                        gggaggGGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCCAAAAAAATcac                  >  2:541456/2‑138 (MQ=255)

                                                                                                                        aggaggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCCATACAAAATAGGTGACATATAGCCGCAAACATAAAAATccc                  >  2:228734/1‑140 (MQ=255)

                                                                                                                         ggaggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATcccc                 <  1:420496/140‑1 (MQ=255)

                                                                                                                          gaggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                <  2:375683/140‑1 (MQ=255)

                                                                                                                              tgtA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACt            <  1:208517/140‑1 (MQ=255)

                                                                                                                               gtA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGAAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCACCTCACTa           >  1:433186/1‑140 (MQ=255)

                                                                                                                                    aa‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGc       <  1:359776/140‑1 (MQ=255)

                                                                                                                                         g‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa    >  2:255216/1‑140 (MQ=255)

                                                                                                                                         g‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa    >  1:17989/1‑140 (MQ=255)

                                                                                                                                           tgtt‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTatttgcggt   <  1:601680/137‑9 (MQ=255)

                                                                                                                                            taT‑GCTTATTTTTGTTCACATCATAGCACCAGTCATCAGTGGCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATa  <  1:301929/139‑1 (MQ=255)

CGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATA  >  CP000730/1929733‑1930008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`