BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,930	T→A	41.6%	intergenic (‑1013/‑155)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,930	0	T	A	41.6%	63.2 / 151.8	127	intergenic (‑1013/‑155)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (35/39); new base A (34/18); total (70/57)
Fisher's exact test for biased strand distribution p-value = 4.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

Only 100 of 129 total aligned reads displayed.

TTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929794‑1930068

ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                               >  1:444181/7‑140 (MQ=255)

 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                              >  2:262819/6‑140 (MQ=255)

 tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                              >  2:223806/6‑140 (MQ=255)

  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGATGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                             >  1:297137/5‑140 (MQ=255)

  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                             >  2:250494/5‑140 (MQ=255)

  cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                             >  1:262654/5‑140 (MQ=255)

   acagatagtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                            <  2:152124/132‑1 (MQ=255)

    ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTg                                                                                                                                            >  2:156940/1‑140 (MQ=255)

    cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAAAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGGTAACAACATAGGACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTgg                                                                                                                                           >  1:337951/3‑140 (MQ=255)

      gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                                          <  2:113652/140‑1 (MQ=255)

      gatgatATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                                          <  1:110705/140‑1 (MQ=255)

        tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCtg                                                                                                                                        <  2:576950/140‑2 (MQ=255)

         ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                                      <  1:426444/138‑4 (MQ=255)

         ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTACTATACTTATTGGCTTAg                                                                                                                                      <  2:335683/138‑1 (MQ=255)

          ttataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:615958/139‑1 (MQ=255)

          gttaaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:332591/136‑1 (MQ=255)

          atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                                      <  2:495638/140‑1 (MQ=255)

           tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    >  2:558378/1‑140 (MQ=255)

           tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAA‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCATTCATCAGTGGCTTTTCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    >  2:157312/1‑140 (MQ=255)

            cgatctatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTGACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                   >  2:6591/8‑139 (MQ=255)

            ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCTGTTGCGTATTATACTTCTTGGcggagaat                                                                                                                                   >  2:135965/1‑133 (MQ=255)

            ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                   >  1:374131/1‑139 (MQ=255)

             taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATAATAGCACCAGTTATCAGTGGCTGTGCCAATGCATTTTTTTCTTATTGGCTAAGTAg                                                                                                                                   <  1:28062/140‑1 (MQ=255)

              aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                                 <  1:540592/140‑4 (MQ=255)

               ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCT‑TTA‑G‑T‑TCTTAAAGCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                                <  1:154182/140‑1 (MQ=255)

               ataCTAGAGTAGCCTATCTCTCAGGCAAAAATT‑‑GAAGAAGAGAGGAGGTGAATTAA‑T‑T‑TGAA‑AAGCATTTTCAGTTGCATCAGATCACCAGTCATCAGTGGCTGTGGTGTTGCGTATGATACTTATTGGCTTAGTAAACg                                                                                                                                <  2:657606/140‑1 (MQ=255)

                 agtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTACTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                               <  1:116665/140‑1 (MQ=255)

                  atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGACGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                                             >  2:433154/2‑140 (MQ=255)

                   tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                                            >  2:353430/1‑139 (MQ=255)

                      agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCATTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                                          >  2:371126/1‑140 (MQ=255)

                      agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                         >  1:644172/1‑136 (MQ=255)

                      agTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                                          <  1:173036/140‑1 (MQ=255)

                       gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                        >  1:631400/1‑135 (MQ=255)

                       gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                        >  1:438656/1‑135 (MQ=255)

                       gTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGATCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATCCTTATTGGCTTAGTAAACGCaaaaaat                                                                                                                        >  2:188322/1‑135 (MQ=255)

                       gTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                        >  2:251746/1‑135 (MQ=255)

                        tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑aa                                                                                                                      >  1:284975/1‑140 (MQ=255)

                        tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                                       >  2:540239/1‑134 (MQ=255)

                          gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACTCAATAC‑‑aa‑‑aa                                                                                                                  >  1:467819/1‑140 (MQ=255)

                          gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                                                                                     >  1:186500/1‑132 (MQ=255)

                           cctctctttacggCGTAAATTGAGACCCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGTT‑GGTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGCAATAC‑‑AA‑‑AAt                                                                                                                 <  2:179563/129‑1 (MQ=255)

                           ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AAt                                                                                                                 >  1:264990/1‑140 (MQ=255)

                           ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                    >  1:53523/1‑131 (MQ=255)

                           ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                    >  2:324772/1‑131 (MQ=255)

                            cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                                   >  2:100951/1‑130 (MQ=255)

                            cTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCCGTCGATGTGCTGTTGCGTATTATACGTATTGGCTTAGTAAACGCaacaaataatta                                                                                                                   <  2:304254/140‑11 (MQ=255)

                             tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTCC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTAGCTTAGTAAACACaacaagtaattta                                                                                                                  >  1:649359/1‑129 (MQ=255)

                             tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                  >  1:418785/1‑129 (MQ=255)

                             tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                  >  1:307337/1‑129 (MQ=255)

                              aTCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                 <  2:214678/140‑13 (MQ=255)

                              aTCTCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑C‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                 <  2:532574/140‑13 (MQ=255)

                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattact                                                                                                                 >  2:624595/1‑128 (MQ=255)

                              aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                 >  1:314945/1‑128 (MQ=255)

                                ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                                               <  2:660952/140‑15 (MQ=255)

                                ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCT‑AATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                                               >  2:607649/1‑126 (MQ=255)

                                     aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ata                                                                                                      >  2:53056/1‑140 (MQ=255)

                                          tccaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGcc                                                                                                <  2:654316/137‑1 (MQ=255)

                                             aattgacgcctgagagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCa                                                                                            <  1:45457/129‑1 (MQ=255)

                                             aTTTAGGCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCa                                                                                            <  2:117695/140‑1 (MQ=255)

                                               ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTTACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCAcc                                                                                          >  2:355254/1‑140 (MQ=255)

                                                     gCAGATACGAGTTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATCGGCTAAGTCGCCGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATaaa                                                                                    <  1:657444/140‑1 (MQ=255)

                                                       agagagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATaaaaa                                                                                  <  1:546191/140‑1 (MQ=255)

                                                        gagagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GAACCAATAAAAAt                                                                                 >  1:373733/1‑140 (MQ=255)

                                                         agagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATc                                                                                <  1:581282/140‑1 (MQ=255)

                                                         agagGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATc                                                                                >  1:442154/1‑140 (MQ=255)

                                                           aggaggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCCATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCAAACATAAAAATccc                                                                              >  2:228734/1‑140 (MQ=255)

                                                            ggaggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATcccc                                                                             <  1:420496/140‑1 (MQ=255)

                                                             gaggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCt                                                                            <  2:375683/140‑1 (MQ=255)

                                                               ggTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTCTTATTGGCTAAGTAGAGGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCACTAAAAATCCCCCCa                                                                          >  2:293383/1‑140 (MQ=255)

                                                                 tgtA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACt                                                                        <  1:208517/140‑1 (MQ=255)

                                                                  gtA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTa                                                                       >  1:634105/1‑140 (MQ=255)

                                                                  gtA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGAAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCACCTCACTa                                                                       >  1:433186/1‑140 (MQ=255)

                                                                  gtA‑TAA‑G‑G‑TGAT‑GCTTATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGAAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTa                                                                       >  1:354392/1‑140 (MQ=255)

                                                                        a‑G‑G‑TGAT‑GCTTATTTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑CCCCGGTCAC‑AGAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                                  <  2:607591/140‑1 (MQ=255)

                                                                            g‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaaa                                                                >  1:17989/1‑140 (MQ=255)

                                                                               taT‑GCTTATTTTTGTTCACATCATAGCACCAGTCATCAGTGGCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATa                                                              <  1:301929/139‑1 (MQ=255)

                                                                                    cTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGa                                                          >  1:60442/1‑140 (MQ=255)

                                                                                     ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGCC‑ATAT‑AGCC‑GCACCAATACACAACCCCTCACTACCGCAAGTaacgcg                                                         >  2:552250/1‑135 (MQ=255)

                                                                                     ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATCGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATTAAAATCCCCTCACTACCACAAagagagag                                                         >  2:424809/1‑140 (MQ=255)

                                                                                      tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTGTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        >  1:457144/1‑140 (MQ=255)

                                                                                      tATTTCCGTTCCTATTATAGCCCCAGTCATCAGTGGCTGTGCCATTTCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        <  2:355065/140‑1 (MQ=255)

                                                                                        ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                                      <  1:130121/140‑1 (MQ=255)

                                                                                              ttCACATCATAGCACCAATCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgt                                                >  2:25765/1‑140 (MQ=255)

                                                                                                    tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCCAAAAGTGAGGGGATTGGTGTATaa                                          <  2:643649/140‑1 (MQ=255)

                                                                                                    tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGAATaa                                          >  2:665917/1‑140 (MQ=255)

                                                                                                     catAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAg                                         <  1:146383/140‑1 (MQ=255)

                                                                                                       tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGgtgcat                                            <  1:102347/140‑4 (MQ=255)

                                                                                                           aCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATa                                   >  1:185855/1‑140 (MQ=255)

                                                                                                               gTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTa                               >  1:562388/1‑140 (MQ=255)

                                                                                                                 catcaGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAT‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTAGCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                             <  1:53056/140‑1 (MQ=255)

                                                                                                                  atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                            <  1:240119/140‑1 (MQ=255)

                                                                                                                  atcaGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTATCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                            <  1:343325/140‑1 (MQ=255)

                                                                                                                    caGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑CA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTc                          >  1:221683/1‑140 (MQ=255)

                                                                                                                           tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCt                   >  2:374244/1‑140 (MQ=255)

                                                                                                                           tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATACGGAGGGGGTAGGTGTATAAGTAAATACTTTTTTTTGTTTTCt                   >  2:370638/1‑140 (MQ=255)

                                                                                                                             tgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAACACTTATTTTCGTTGTCTTa                 <  1:371126/140‑1 (MQ=255)

                                                                                                                                cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGAGTATAAGTAAATACTTATTTTCGTTGTCTTAAtt              >  1:611717/1‑140 (MQ=255)

                                                                                                                                cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAAAAAAAAACCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAAACTTATTTTCGTTGTCTTAAtt              >  1:596654/1‑140 (MQ=255)

                                                                                                                                 aTTGCGTTTTTTTCGTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTTCCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATta             <  1:180334/140‑1 (MQ=255)

                                                                                                                                    gCGTTTTTTTCTCTTTGGCTAAGTAGACTCAATAC‑‑AA‑‑AATAGGGCAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTTCCGTAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTTTCTTAATTATAc          <  2:43113/140‑1 (MQ=255)

TTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAT‑GCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929794‑1930068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`