BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	22.7%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	22.7%	414.3 / 129.8	294	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (96/127); new base A (30/37); total (127/167)
Fisher's exact test for biased strand distribution p-value = 8.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

Only 100 of 298 total aligned reads displayed.

ATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG  >  CP000730/35801‑36079

ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTACTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                           >  1:46612/4‑140 (MQ=11)

ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                           <  2:14986/137‑1 (MQ=11)

  ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTTATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                                                         <  1:595201/139‑1 (MQ=11)

  cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                           <  1:635511/140‑1 (MQ=255)

   taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                        <  2:45730/140‑1 (MQ=11)

    aataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAATATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                                                       >  1:228945/1‑140 (MQ=11)

                tggttaaatttcAAAGCAAAAAAATATAGATAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                                                                                             <  2:546926/129‑1 (MQ=1)

                                              tatccACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAg                                                                                               <  1:15170/137‑1 (MQ=1)

                                                       atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTGAATACGTTTTAAAGTATTCTTTg                                                                                      >  2:531082/4‑140 (MQ=1)

                                                       atatATCAGGTCATTGTTCTTTTAACTTGCAAGCATGATGCTAATTTCCTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATCGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTg                                                                                      <  1:320431/137‑1 (MQ=1)

                                                             caggTCCGTGTTGGCTTAACTTGCTAGCGTGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATTTGAAGAGACCTGCGGCTCTTTCTATATATAGCGTAAATACCTTCAATACCGTTTAATCTACTCTTTGCTGTAt                                                                                <  2:276759/137‑1 (MQ=1)

                                                                   aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                                                          >  1:18135/1‑140 (MQ=11)

                                                                   aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAGATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                          <  1:656331/140‑1 (MQ=2)

                                                                    gttttcgtctAACTTGCTAGCAAGATGCTAATTTCGTGGCATGGCTAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                         <  1:10599/131‑1 (MQ=11)

                                                                    gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                         >  1:284000/1‑140 (MQ=11)

                                                                     tgtTCGCTTAACTTGCTAGCCTTTGGCTTATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                        >  2:642331/1‑140 (MQ=11)

                                                                     tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                        >  1:494598/1‑140 (MQ=17)

                                                                     tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                        >  1:306929/1‑140 (MQ=17)

                                                                      gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                       <  1:360350/140‑1 (MQ=18)

                                                                       ttCGCTTAACTTTCTAGCCTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATATAGCATAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                      <  2:57776/140‑1 (MQ=11)

                                                                        tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                     >  2:391978/1‑140 (MQ=12)

                                                                        tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                     <  2:628678/140‑1 (MQ=16)

                                                                         cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATGGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                                                    >  1:27794/4‑140 (MQ=11)

                                                                         cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                    >  2:349203/1‑140 (MQ=17)

                                                                           tttAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTGTTTGCTGTATTGATACTTTGATAc                                                                  <  2:229148/139‑1 (MQ=255)

                                                                            ttAACTTGCTAGCATGATGCTAATTTCTTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                 <  1:214402/140‑1 (MQ=18)

                                                                            ttAACTTGCTAGCATGATGCTAATTTCTCGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                 <  1:547860/140‑1 (MQ=255)

                                                                              aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                               <  1:53145/140‑1 (MQ=18)

                                                                               aCTTGCTAGCATGAAGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                              <  1:416740/140‑1 (MQ=11)

                                                                                cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTTGt                                                             >  2:174310/1‑140 (MQ=17)

                                                                                cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                             <  1:652014/140‑1 (MQ=18)

                                                                                cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                             <  2:598111/140‑1 (MQ=18)

                                                                                  tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                           <  1:604029/140‑1 (MQ=18)

                                                                                   tcTAGCATGATTGTAATTTCGTGGCGTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACAATAAATAACTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                          <  2:437607/139‑1 (MQ=11)

                                                                                   gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                          <  2:545023/140‑1 (MQ=11)

                                                                                   gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                          >  1:247430/1‑140 (MQ=17)

                                                                                    cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATGTTGtcttt                                                         >  2:259007/1‑140 (MQ=11)

                                                                                     tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                        >  1:590772/1‑140 (MQ=11)

                                                                                     tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATAACTTTTAAAGAATGGTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                        >  1:451232/1‑140 (MQ=11)

                                                                                     tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                        >  2:88740/1‑140 (MQ=17)

                                                                                     tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAAACTTTGATACCTTGtctttc                                                        >  2:268720/1‑140 (MQ=17)

                                                                                      aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                       <  1:78582/140‑1 (MQ=18)

                                                                                       gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                      >  1:29451/1‑140 (MQ=18)

                                                                                       gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                      <  2:627672/140‑1 (MQ=18)

                                                                                        cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                     >  2:418175/1‑140 (MQ=18)

                                                                                         atgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                                                    >  1:538261/1‑140 (MQ=11)

                                                                                          tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATAGCtttcataagtgacg                                                   >  2:136003/1‑128 (MQ=11)

                                                                                            atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                 >  1:558252/1‑140 (MQ=35)

                                                                                                tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                             >  1:336157/1‑140 (MQ=255)

                                                                                                tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACTTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                             <  1:269572/140‑1 (MQ=255)

                                                                                                 aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACGTTCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                            >  1:627661/1‑140 (MQ=11)

                                                                                                  ttgtCGGGGCATGGCGAAAATCCGTAGATCTGAAGTGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGTCTTGTCTTTCTTACTTTAATATg                                           <  2:665054/137‑1 (MQ=11)

                                                                                                     tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGGTGTCTGGTCTTTCTTACTTTAATATGACg                                        <  1:159640/140‑1 (MQ=11)

                                                                                                     tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                        <  1:251520/140‑1 (MQ=37)

                                                                                                       gTGGCTTGGGGAAAATCCGTAGATCTAAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTACTTACTTTAATATGaaaag                                      <  2:113425/140‑5 (MQ=11)

                                                                                                        tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                     >  2:44750/1‑140 (MQ=37)

                                                                                                         ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                    <  2:563362/140‑1 (MQ=255)

                                                                                                         ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGATTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTGTTTCTGGCTCTAATGTGACGGTgt                                    >  2:497531/1‑139 (MQ=11)

                                                                                                          gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGATTCTTTTTATATAGAGCGTAACTCCACTCAATACCTTTTAAAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                   >  1:461007/1‑140 (MQ=11)

                                                                                                              ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                               <  1:102097/140‑1 (MQ=255)

                                                                                                              ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                               <  1:134736/140‑1 (MQ=255)

                                                                                                               ggtaaaaTCCGTACATCTGAAGAGACCTTCGGTTCTTTTTATATAGATCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                              <  2:291469/137‑1 (MQ=14)

                                                                                                               gCGAAAATCCGTAGATCTGAAGAGACCTGTGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                              <  1:392957/140‑1 (MQ=255)

                                                                                                                ggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAGGGTGATCTTGCt                             >  2:480440/2‑140 (MQ=11)

                                                                                                                ggAAAATCCGTACATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                             <  2:317341/139‑1 (MQ=255)

                                                                                                                cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTCATTTAATATGACGGTGATCTTGCt                             >  1:236839/1‑140 (MQ=255)

                                                                                                                cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGTTCTTGCt                             >  1:323557/1‑140 (MQ=255)

                                                                                                                 gAAAATCCGTAGATTTGATGATTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATTCCTTTTAAGGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                            <  1:312505/140‑1 (MQ=14)

                                                                                                                 gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                            <  1:444062/140‑1 (MQ=255)

                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATGTTGCTaaa                          >  2:163437/1‑140 (MQ=255)

                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                          >  2:468455/1‑140 (MQ=255)

                                                                                                                   aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTCGCTCaa                          <  1:499595/140‑1 (MQ=255)

                                                                                                                    aaTCCTTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCCTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                         <  1:119374/140‑1 (MQ=255)

                                                                                                                     aTCCGTAGATCTGAAGATACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATCCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                        <  2:374282/140‑1 (MQ=14)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                        >  1:572318/1‑140 (MQ=37)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTCAATATGACGGTGATCTTGCTCAATg                        >  1:407238/1‑140 (MQ=255)

                                                                                                                     aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATg                        >  2:238670/1‑140 (MQ=14)

                                                                                                                      tCCGTAGATCTTAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                       <  1:663396/140‑1 (MQ=255)

                                                                                                                        cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                     >  1:396118/1‑140 (MQ=37)

                                                                                                                        cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAATTTAATATGAAGGTGGTCTTGCTCAATGAgg                     >  2:196154/1‑140 (MQ=255)

                                                                                                                         gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTATGATACCTTGTCTTTCTTTCTTTAATATGACGGAGATCTTACTCAATGAGGt                    >  1:87842/1‑140 (MQ=11)

                                                                                                                          tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTGGATACCTTGTCTTTCTTACTTTAATATGACGCTGATCTTGCTCAATGAGGtt                   >  1:382461/1‑140 (MQ=255)

                                                                                                                          tAGATCTGAAGAGACCTACTGTTCTTTTTATATAGAGCATAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                   <  2:245089/140‑1 (MQ=14)

                                                                                                                          tAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTGATATGACGGTGATCTTGCTCAATGAGGtt                   <  2:610087/140‑1 (MQ=11)

                                                                                                                           aGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATTTTGTCTCTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                  <  1:368/140‑1 (MQ=14)

                                                                                                                             atcttaagatctcTGCGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTCTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                <  2:58819/128‑1 (MQ=11)

                                                                                                                             aTCTGTAGTGACCTGCGGTTTTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAAATGACGGTGATCTTGCTCAATGAGGTTAtt                <  1:142928/140‑1 (MQ=14)

                                                                                                                             aTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTGAAGTATTCTTTGCCGTATTGTTACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                <  1:509641/140‑1 (MQ=11)

                                                                                                                               cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGTCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa              <  1:11153/140‑1 (MQ=255)

                                                                                                                                tGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg             <  2:356359/140‑1 (MQ=255)

                                                                                                                                tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg             >  2:512182/1‑140 (MQ=255)

                                                                                                                                tGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg             >  2:204934/1‑140 (MQ=255)

                                                                                                                                 gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa            <  1:203122/140‑1 (MQ=255)

                                                                                                                                     agaCCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt        <  2:363884/140‑1 (MQ=255)

                                                                                                                                     agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt        <  2:122629/140‑1 (MQ=255)

                                                                                                                                     agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt        <  2:261221/140‑1 (MQ=255)

                                                                                                                                     agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt        <  2:344394/140‑1 (MQ=255)

                                                                                                                                        ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg     <  1:588752/140‑1 (MQ=255)

                                                                                                                                         cTGCGGTTCTTTTTATCTAGAGCGTAAATACACTCAATAACTTTTAAAGTATTCTTTGCTGTATTGATAATTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa    <  2:33361/140‑1 (MQ=255)

                                                                                                                                           gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTACTCAATGAGGTTATTCAGATATTTCGATg  >  1:117787/1‑140 (MQ=255)

ATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG  >  CP000730/35801‑36079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`