Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,010,3630CT100.0% 167.0 / NA 55C146Y (TGT→TAT) USA300HOU_1861ABC superfamily ATP binding cassette transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/26);  total (29/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGG  >  CP000730/2010234‑2010494
                                                                                                                                 |                                                                                                                                    
gtCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTTAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCa                                                                                                                            <  1:175099/140‑1 (MQ=255)
 tCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAGAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaa                                                                                                                           >  1:576860/1‑140 (MQ=255)
  cAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaaa                                                                                                                          >  2:494527/1‑140 (MQ=255)
  cAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCaaa                                                                                                                          >  2:296089/1‑140 (MQ=255)
   aaTTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAat                                                                                                                         >  1:632926/1‑140 (MQ=255)
        tCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCACTTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAAAATTCa                                                                                                                    >  1:425265/1‑140 (MQ=255)
            aaTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTaa                                                                                                               >  1:406321/1‑140 (MQ=255)
             aTCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGAGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAAc                                                                                                              >  1:622621/1‑140 (MQ=255)
              tgttCCAAAGTAAACGTACACCGTGAAAATGTAAAATGGGGATATCATCAGTGCTGCTAAATCCAATTTCACAGCTAAAAAGAACTTTCTGGACAGTGCAATAATGATTGTTATTTAATCTAACCAAATATTCATTAAcc                                                                                                             <  2:154392/138‑1 (MQ=255)
               cTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTAAATAATATAACCAAATAATTATTAAccc                                                                                                            >  2:100778/1‑140 (MQ=255)
                 tCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCACTCATAATTGTTATATAATCTAACCAAATATTCATTAACCCgg                                                                                                          <  2:607734/140‑1 (MQ=255)
                   cAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGtt                                                                                                        <  2:522419/140‑1 (MQ=255)
                         acccggacacCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATg                                                                                                  <  2:633897/134‑1 (MQ=255)
                         aatcGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATg                                                                                                  <  2:170387/137‑1 (MQ=255)
                         aaaCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATCATTTAACCCGGTTaaaaag                                                                                                  >  2:77573/1‑138 (MQ=255)
                           aCGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGaa                                                                                                >  1:18044/1‑140 (MQ=255)
                             gTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAAt                                                                                              <  1:607662/140‑1 (MQ=255)
                               acacCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCt                                                                                            <  2:456591/140‑1 (MQ=255)
                                    gTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTtttgt                                                                                       >  2:550425/1‑140 (MQ=255)
                                          atgtaaaatgGCAAGATAACCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATCATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTc                                                                                 <  2:155150/140‑1 (MQ=255)
                                           tgtaaaatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAACAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCa                                                                                <  2:456329/140‑1 (MQ=255)
                                                aatgTGAAGATAAACAGTGCTGCTAAAGTAACTTTCACATCTAAATAGAATATTATGTATAGTGCATTAATAATTGTTATATATTCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatc                                                                           <  1:140818/140‑1 (MQ=255)
                                                aatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATTGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatc                                                                           <  1:496769/140‑1 (MQ=255)
                                                aatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatc                                                                           <  2:186262/140‑1 (MQ=255)
                                                aatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatc                                                                           <  2:594515/140‑1 (MQ=255)
                                                aatgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTAAATAATCTAACAAATCATTCCTTAACCAGGTTAAAATGAAATCTTTTGTTTGTTCAAcata                                                                           >  1:252891/1‑139 (MQ=255)
                                                  tgGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                         <  2:511334/140‑1 (MQ=255)
                                                  tgGGAAGAGAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAAcatcat                                                                         <  2:149307/140‑1 (MQ=255)
                                                    ggAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTa                                                                       >  2:664117/1‑140 (MQ=255)
                                                        gATAAATAGTTCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCa                                                                   <  2:213162/140‑1 (MQ=255)
                                                        gATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCa                                                                   <  1:614699/140‑1 (MQ=255)
                                                        gATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCa                                                                   <  1:347337/140‑1 (MQ=255)
                                                         aTAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCAc                                                                  >  1:426226/1‑140 (MQ=255)
                                                            aaCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCActct                                                               >  2:226799/1‑140 (MQ=255)
                                                             aCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTa                                                              <  1:570719/140‑1 (MQ=255)
                                                             aCAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAACCTTTTGTGTTTTCAACATCATTAATCACTCTa                                                              <  2:142902/140‑1 (MQ=255)
                                                                  gctgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCACCATCATTAATCACTCTAGctat                                                         >  2:270386/1‑140 (MQ=255)
                                                                  gctgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGatat                                                         >  1:156040/1‑140 (MQ=255)
                                                                    tgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATCGTGCAATACTAATTGTTATATAATCTAACAAAAAATTCATCTAAAGCCTAAAGAATAAATTTATTGTTTGTTCAACATCATTAATCACTCTAGATATTa                                                       >  1:289323/1‑140 (MQ=255)
                                                                    tgctAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATATTTTGTTTGTTAAACATCATTAATCACTCTAGATATTa                                                       >  1:93209/1‑140 (MQ=255)
                                                                          aGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGAc                                                 >  1:472206/1‑140 (MQ=255)
                                                                                         tAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATATACTCTAACCAAATATTCATTACCCCGGTTAAACTGAAAGCTTTCGTTTCTTCCACATCATCAAGCACCCTCGATATCACTTGACCTACtctatcatcac                                  >  2:617620/1‑130 (MQ=255)
                                                                                                aaCATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACGCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTAGTAGCATaaaa                           <  1:483461/140‑1 (MQ=255)
                                                                                                  cATTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtc                         >  2:105072/1‑140 (MQ=255)
                                                                                                   aTTATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtct                        >  2:375436/1‑140 (MQ=255)
                                                                                                    ttATGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTCAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAAtctc                       <  2:476545/140‑1 (MQ=255)
                                                                                                    ttAGGGATAGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTCAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTTCTTGATTATTAGCATAAAAtctc                       <  2:476621/140‑1 (MQ=255)
                                                                                                         gATAGTTCAATAATAATTGTTATATAATCTAGCCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTATTTAACATCATTAAGCACTCTTGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACt                  <  2:202/140‑1 (MQ=255)
                                                                                                           tAGTGCAATAATAAATGTTATATAATCTAACCAGATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCTTTAATTACTCTAGTTATTACTTGACCTACTTGATTATCAGCATAAAATCTCGCACTTa                <  1:405445/140‑1 (MQ=255)
                                                                                                            aGTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTTTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACttca               >  1:663415/1‑138 (MQ=255)
                                                                                                             gTGCAATAATAATTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTaaa              >  2:564519/1‑140 (MQ=255)
                                                                                                                cAATAATAATTGTTATATAATCTAACCAAATATTCCTTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAATCt           >  1:396239/1‑140 (MQ=255)
                                                                                                                       aatTGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAAt    >  1:627532/1‑140 (MQ=255)
                                                                                                                         ttGTTATATAATCTCACCAAATATTCAATAATCTGGTTAACATGAAATCTTTTGTTTGTTCAACATCATTAATCCCTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATgg  >  2:276813/1‑140 (MQ=255)
                                                                                                                         ttGTTATATAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATAACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATgg  >  1:218607/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                    
GTCAATTTTCTTAATCTTCCAAAGAAAACGTACACCGTTAAAATGTAAAATGGGAAGATAAACAGTGCTGCTAAAGTCAATTTCACATCTAAAAAGAACATTATGGATAGTGCAATAATAATTGTTATACAATCTAACCAAATATTCATTAACCCGGTTAAAATGAAATCTTTTGTTTGTTCAACATCATTAATCACTCTAGATATTACTTGACCTACTTGATTATTAGCATAAAATCTCGCACTTAAAGCTTGTAAATGG  >  CP000730/2010234‑2010494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: