BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	58.9%	E194E (GAA→GAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	58.9%	198.4 / inf	417	E194E (GAA→GAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (88/83); new base C (118/124); total (209/208)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

Only 100 of 421 total aligned reads displayed.

ATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAG  >  CP000730/35840‑36109

       tatccACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAg                                                                                                                             <  1:15170/137‑1 (MQ=1)

                      caggTCCGTGTTGGCTTAACTTGCTAGCGTGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATTTGAAGAGACCTGCGGCTCTTTCTATATATAGCGTAAATACCTTCAATACCGTTTAATCTACTCTTTGCTGTAt                                                                                                              <  2:276759/137‑1 (MQ=1)

                            aGTGTTCGTTTAACTTGGTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc                                                                                                        <  1:231026/140‑1 (MQ=11)

                              tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                      >  2:478886/1‑140 (MQ=255)

                              tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                      >  1:494598/1‑140 (MQ=17)

                               gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                                                     <  1:645293/140‑1 (MQ=11)

                                 tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAAACCTACGGTTCTTTTTATATAGTGTGTAAATACATTCAATACCTTTTAAAGTATTCTTTGGCGTATTGATACTTTGa                                                                                                   <  1:330195/136‑1 (MQ=11)

                                     ttAACTTGGTAGCTTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                               <  1:386453/140‑1 (MQ=255)

                                     ttAACTTGCTAGCATGATGCTAATTTCTTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                               <  1:214402/140‑1 (MQ=18)

                                     ttAACTTGCTAGCATGATGCTAATTTCTCGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                               <  1:547860/140‑1 (MQ=255)

                                       aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                             >  2:612850/1‑140 (MQ=32)

                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTATTTGCGGTGTTGAGACTTTGATATCTTGt                                                                                           >  1:312821/1‑140 (MQ=11)

                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                           <  1:373544/140‑1 (MQ=18)

                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                           <  2:598111/140‑1 (MQ=18)

                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                                                           <  1:652014/140‑1 (MQ=18)

                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACttct                                                                                           >  2:635444/1‑138 (MQ=12)

                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTACTCACCCGAGTGATACTTTGATACCTTGt                                                                                           <  1:159932/140‑1 (MQ=11)

                                            tcTAGCATGATTGTAATTTCGTGGCGTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACAATAAATAACTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                        <  2:437607/139‑1 (MQ=11)

                                            gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                                                        <  2:545023/140‑1 (MQ=11)

                                              tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATAACTTTTAAAGAATGGTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                                                      >  1:451232/1‑140 (MQ=11)

                                               aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                     <  1:78582/140‑1 (MQ=18)

                                                gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                                                    <  2:627672/140‑1 (MQ=18)

                                                 cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATAACTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                                                                                   >  1:276510/1‑140 (MQ=11)

                                                 cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                   >  1:503188/1‑140 (MQ=18)

                                                   tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTatt                                                                                 >  1:118729/1‑138 (MQ=14)

                                                     attgtAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAAAGACCTTCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                               <  2:467755/136‑1 (MQ=11)

                                                     atgCTAATTTCGTGTCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                               <  2:205925/140‑1 (MQ=255)

                                                     atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                               >  1:558252/1‑140 (MQ=35)

                                                     atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                               >  2:261170/1‑140 (MQ=35)

                                                         tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAATTTAATATCTTGTCTTTCTTACTTTAatt                                                                           >  1:600465/1‑139 (MQ=11)

                                                         tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                           <  1:250578/140‑1 (MQ=37)

                                                         tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                           >  2:2020/1‑140 (MQ=25)

                                                           aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAATATg                                                                         >  2:57899/1‑140 (MQ=255)

                                                           aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                         >  1:141332/1‑140 (MQ=21)

                                                                gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                    >  1:26738/1‑140 (MQ=38)

                                                                gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                    <  1:292437/140‑1 (MQ=255)

                                                                gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCCGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAATATTCTTTGCTGTATTGATACTTTGATAACTTGTCTTTCTTACTGTAATATGACGGt                                                                    >  1:114632/1‑140 (MQ=255)

                                                                 tGGCAGGGCGAAAATCCTTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                                                   <  2:247857/140‑1 (MQ=255)

                                                                    cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATTCTCTCTATTTCTTTTAATGTTTTCTTTGCTGTTTTGATACTTTGATACCTTGTCTTTCTTTCTTTAATATGACGGTGATc                                                                <  1:366699/140‑1 (MQ=14)

                                                                      gcgggAAAAGCCGTAGGTCCGAAGAGACCTACGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                              <  2:536925/136‑1 (MQ=11)

                                                                       ggCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                             <  1:102097/140‑1 (MQ=255)

                                                                       ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGCTACCTTGTCTTTCTTACTTTAATATGACGGTCATCTTg                                                             >  2:609582/1‑140 (MQ=255)

                                                                       ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                                                             <  2:556291/140‑1 (MQ=255)

                                                                         ggAAAATCCGTACATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                           <  2:317341/139‑1 (MQ=255)

                                                                         cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCt                                                           >  2:151548/1‑140 (MQ=255)

                                                                             aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                       <  1:412169/140‑1 (MQ=39)

                                                                             aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCact                                                       >  2:565920/1‑138 (MQ=255)

                                                                              aTCCGTAGATCTGAAGATACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATCCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                      <  2:374282/140‑1 (MQ=14)

                                                                              aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATg                                                      >  2:238670/1‑140 (MQ=14)

                                                                               tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                     <  1:536485/140‑1 (MQ=38)

                                                                                 cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGATGATCTTGCTCAATAAgg                                                   >  2:167978/1‑140 (MQ=255)

                                                                                   tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                                                 >  1:24416/1‑140 (MQ=38)

                                                                                      aTCTGAATAGGCCTGCGTTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                              <  2:116088/140‑1 (MQ=255)

                                                                                      aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                              <  1:629093/140‑1 (MQ=255)

                                                                                         tGAATACACTTGCGGTTCTTTTTATATAGAGTGTAAATACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                           <  1:282638/140‑1 (MQ=11)

                                                                                         tGAAGTGACCTGCGGTTCTTTTTATATAGAGCGTAAATACTCTCAATACCTTTTTAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTTTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                           <  1:303232/140‑1 (MQ=11)

                                                                                         tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                           >  2:512182/1‑140 (MQ=255)

                                                                                          gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTATCTTTCTTAATTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                          <  1:126454/140‑1 (MQ=255)

                                                                                          gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                          <  1:203122/140‑1 (MQ=255)

                                                                                          gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                          <  2:108877/140‑1 (MQ=38)

                                                                                             gagaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCGTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat                                       <  1:588946/140‑1 (MQ=255)

                                                                                              agaCCTGCGGTTCTTTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                      <  2:363884/140‑1 (MQ=255)

                                                                                              agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                      <  1:393980/140‑1 (MQ=38)

                                                                                               gaCCTGCGGTTCTTTTTATATAGAGCGTCAACGCACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTTTTTCTTACTTTAATATGACGGTGATCTTGCTCAAATAGGTTATTCAGATAttt                                     >  1:321895/1‑140 (MQ=11)

                                                                                               gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                     >  1:576547/1‑140 (MQ=38)

                                                                                                     cGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                               <  1:165207/140‑1 (MQ=38)

                                                                                                      ggTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGGTATTTCGATGTa                              >  2:620487/1‑140 (MQ=255)

                                                                                                      ggTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATGAGTTTATTCAGATATTTCGATGTa                              >  2:444874/1‑140 (MQ=255)

                                                                                                      ggTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATCTTTCGATGTa                              >  2:132921/1‑140 (MQ=255)

                                                                                                         tCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGGACaa                           >  2:492450/1‑140 (MQ=255)

                                                                                                          cTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCTATGTACAAt                          <  2:346942/140‑1 (MQ=255)

                                                                                                           tttttATATAGAGCGTAAATATACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTTCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                         <  1:462173/140‑1 (MQ=255)

                                                                                                           gttttATATAGAGCATAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATg                         <  2:122014/139‑1 (MQ=255)

                                                                                                             tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                       <  1:608387/140‑1 (MQ=255)

                                                                                                              ttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTAGATGTACAATGACa                      >  1:106470/1‑140 (MQ=255)

                                                                                                              ttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATggca                      <  2:296060/140‑4 (MQ=255)

                                                                                                               tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAg                     <  2:264490/140‑1 (MQ=255)

                                                                                                                atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTATTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                    <  1:186927/140‑1 (MQ=255)

                                                                                                                 tataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATGTAAAATGACAGTc                   >  2:423473/1‑140 (MQ=255)

                                                                                                                   taGGGCGTAAATACATTCTATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAg                 <  2:67470/140‑1 (MQ=255)

                                                                                                                      agCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCTATGTACAATGGCAGTCAGgtt              >  2:230443/1‑140 (MQ=255)

                                                                                                                      agCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt              >  2:646937/1‑140 (MQ=255)

                                                                                                                       tgttAAATACATTCAATACCTTTGAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt             <  2:350967/137‑1 (MQ=14)

                                                                                                                         gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaa           >  1:339252/1‑140 (MQ=40)

                                                                                                                          tAAATACACTTAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTTATCTTTCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag          <  2:360555/140‑1 (MQ=14)

                                                                                                                          tAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGATGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag          <  1:67175/140‑1 (MQ=255)

                                                                                                                          tAAAAACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATCTGACGGTGATCTTGCTCAATGAGGTTCATAAGATATTTCGATGTACAATGACAGGCAGgtttaag          >  2:281408/1‑140 (MQ=14)

                                                                                                                           aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagt         >  1:473959/1‑140 (MQ=255)

                                                                                                                            aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt        <  1:66035/140‑1 (MQ=255)

                                                                                                                            aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt        >  2:393900/1‑140 (MQ=255)

                                                                                                                            aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt        >  1:400025/1‑140 (MQ=255)

                                                                                                                             aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt       <  1:525427/140‑1 (MQ=255)

                                                                                                                             aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGggttaagttt       >  2:84685/1‑140 (MQ=255)

                                                                                                                              tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta      >  1:62313/1‑140 (MQ=40)

                                                                                                                              tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta      >  1:444756/1‑140 (MQ=255)

                                                                                                                              tACATTCAATACCTTTTAAACTATTCTTTGCTGTATTGATACTTTGATACCTCGTCTTGCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGCACAATGACAGTCAGgtttaagttta      >  1:406504/1‑140 (MQ=255)

                                                                                                                              tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGCTACTTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta      >  2:404742/1‑140 (MQ=255)

                                                                                                                               acgttCAATGCCTTTTAACGTATTCTTTGCTTTATGGAGACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa     <  2:336889/137‑1 (MQ=14)

                                                                                                                               aCATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaggtttaa     >  1:423796/1‑140 (MQ=255)

                                                                                                                                  ttCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTGCAATTGCAGTCAggtttaggtttacagg  >  2:187939/1‑136 (MQ=14)

ATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAG  >  CP000730/35840‑36109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`