Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,324,256 (A)9→8 100% coding (1028/2010 nt) mutL → DNA mismatch repair protein MutL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,324,2480A.91.7% 91.1 / 0.5 24coding (1020/2010 nt)mutLDNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (11/11);  total (12/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGGAAGAAAATCAAA  >  CP000730/1324121‑1324384
                                                                                                                               |                                                                                                                                        
ttCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGt                                                                                                                            <  1:200206/141‑1 (MQ=255)
ttCATCAAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGt                                                                                                                            <  1:4536/141‑1 (MQ=255)
       aacaaAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTAcat                                                                                                                     <  1:328859/141‑1 (MQ=255)
        acaaAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATCAAGTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTAcatt                                                                                                                    >  1:269664/1‑141 (MQ=255)
                 gAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGaaa                                                                                                           >  1:368067/1‑140 (MQ=255)
                   aGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTAGATTAATTCCTACAAATACTTTGGATGATGTGCCAAAAAAAATAAAGAATTACATTCATTCAaacac                                                                                                         >  2:42707/1‑140 (MQ=255)
                             acAAACGAAGAGTAACTATACCCATGGATTGTGAGAAAAATACAAGAAGCATTCAAAGACCGTATATTAATTCCTAAAAATAACTTGCATTATGAGAAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCg                                                                                               <  1:181021/140‑1 (MQ=255)
                              cAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAATATAACTTGGATTATGTGCAAGACAACATAAAGTATTACATTCATTCGAACAACAAAAAATCGa                                                                                              >  1:267713/1‑141 (MQ=255)
                                aaaGAAGAGCAACTATACCAATTGATTGTGAGCAAAATTCACGCGCCTATCAAATGCCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAt                                                                                            <  1:89543/141‑1 (MQ=255)
                                aaaGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAAt                                                                                            <  1:320014/141‑1 (MQ=255)
                                        gCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACaa                                                                                    >  1:384890/1‑141 (MQ=255)
                                                       tattgTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACagag                                                                     <  1:336676/140‑1 (MQ=255)
                                                       gagtgggAGCAAAATACAAGAAGCATTTAAAGCCCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACagag                                                                     <  1:176696/135‑1 (MQ=255)
                                                          tgtgAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAAcacaaaaaaa                                                                  >  2:36967/1‑135 (MQ=255)
                                                              aGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGACTAAcc                                                              >  1:126390/1‑141 (MQ=255)
                                                                     tACAATAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGagaa                                                       <  2:116857/141‑1 (MQ=255)
                                                                       cAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGagaaga                                                     <  1:49310/141‑1 (MQ=255)
                                                                        aagaagCATTTAAAGACCGTATATTAATACCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTAATACGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGAc                                                    <  2:14882/141‑1 (MQ=255)
                                                                                 ttAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATc                                           >  1:182884/1‑141 (MQ=255)
                                                                                   aaaGACCGTATATTAATTCCTAAAAATAACTTGGATTAAGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACACCAAAAAATCGAATTTGAAAAAAGACAAAACACAGAAAAAAACAAAGGGAAGCCAttttaaaata                                         >  2:334186/1‑135 (MQ=255)
                                                                                      gACCGTATATTAATACCTAAAAATAACTTGGATTATGTTCCAAAAAAAATAAAGGAGTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCGgaag                                      >  2:231017/1‑141 (MQ=255)
                                                                                                      cTAAAAATAACTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGcc                      <  1:261433/141‑1 (MQ=255)
                                                                                                               aCTTGGATTATGTGCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTGATGga             >  1:109334/1‑141 (MQ=255)
                                                                                                                      aatatgtgaaaaaaaaaaTAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGGAAGaaaa       <  2:21073/132‑1 (MQ=255)
                                                                                                                          gtgCCAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGGAAGAAAATCaaa  >  1:19876/1‑141 (MQ=255)
                                                                                                                               |                                                                                                                                        
TTCATCCAACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAAATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCAAAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAAAACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTTATGGAAGAAAATCAAA  >  CP000730/1324121‑1324384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: