| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,041,281 | T→C | 11.5% | intergenic (‑364/+219) | USA300HOU_1897 ← / ← USA300HOU_1899 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,041,281 | 0 | T | C | 11.5% | 79.0 / 4.7 | 35 | intergenic (‑364/+219) | USA300HOU_1897/USA300HOU_1899 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/11); new base C (2/2); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01 | |||||||||||
AAGCTGACAGAAAGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGTTCTTCATTATATAGACT > CP000730/2041157‑2041417 | aaGCTGACAGAAAGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGt < 1:19126/141‑1 (MQ=255) aGCTGACAGAAAGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTc > 1:199992/1‑141 (MQ=255) gccaaaaaGTCATCTTCTTTTTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGAAATTGAATTTATGACCCAGGCTCAGTCTTAt < 1:261974/136‑1 (MQ=25) cAGAAAGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTCAATTTATGACCCAGCCTCAGTCTTAttt > 2:325104/1‑141 (MQ=255) aGAAAGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACCTTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTtc > 1:369150/1‑141 (MQ=255) aaaGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGCATCTATGACCCAGGCTCAGTCTTATTtcat > 2:62886/1‑141 (MQ=255) aGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTCATGACCCAGGCTCAGTCTTATTtcatca > 1:120466/1‑141 (MQ=255) aGTCAGCTTCTACGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTCATGACCCAGGCTCAGTCTTATTtcatca > 1:355388/1‑141 (MQ=255) gCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGACTTTATGACCCAGGCTCCGTCTTATTTCATCATTCtt > 1:41746/1‑141 (MQ=255) ttGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGtt > 1:143890/1‑141 (MQ=255) cTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACCTTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCACCt > 1:331713/1‑141 (MQ=255) cTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGGGCCTAGGACATTGAATTTATGACCCCGGCTCCGTCTTATTTCATTATTCTTAATATCGTTAAAGTCCAACt > 1:62897/1‑141 (MQ=37) gAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGt < 1:330943/141‑1 (MQ=255) ttGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGCCCCCGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATc < 1:56108/141‑1 (MQ=255) tGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCt < 1:326255/141‑1 (MQ=255) aaaGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTCATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAAc < 2:355388/141‑1 (MQ=255) aaaGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTCATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAAc < 2:120466/141‑1 (MQ=255) ttctttcAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACACATACTACCTTGAGATGTaa < 2:86531/141‑2 (MQ=255) ttctttcAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTAc > 2:27043/1‑141 (MQ=255) tcAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGc < 1:376378/141‑1 (MQ=255) cAGTCAACTACAGCCAATATAACCTTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTAAAAAGCt > 1:392191/1‑141 (MQ=255) cAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCt > 2:14422/1‑141 (MQ=255) gTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTg > 2:305920/1‑141 (MQ=255) gTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTg > 1:24232/1‑141 (MQ=255) gTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAAATTGTATCTTAAACAAATACTAATTCAATATGTACAAAGtttt > 2:185358/1‑140 (MQ=255) tCAACTACAGCCAATATAACATTGTAGTGCCTAGGACTTTGAAGTTATGACACAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTAACAATGGCTCTATCTCAATATGTACAAAGCTTGt < 1:191510/141‑1 (MQ=255) gCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTc > 1:302102/1‑141 (MQ=255) aaTATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGc < 2:369150/141‑1 (MQ=255) cttGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTg < 1:245151/140‑1 (MQ=255) tAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGt > 2:242614/1‑141 (MQ=255) tAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGt > 2:368730/1‑141 (MQ=255) ccTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGttcttc < 2:199992/141‑1 (MQ=255) ggACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTAAGCATTTTTTTCCGTTCTTCATta > 1:183992/1‑141 (MQ=255) gAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGTTCTTCATTATATAGAc > 2:323018/1‑141 (MQ=255) aaTTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATCTGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGTTCTTCATTATATAGACt > 2:131821/1‑141 (MQ=255) | AAGCTGACAGAAAGTCAGCTTCTATGTTGGGGCCCCACTAGAATTGAAAAAAGCTTGTTACAAGCGTATTTTCTTTCAGTCAACTACAGCCAATATAACATTGTAGTGCCTAGGACATTGAATTTATGACCCAGGCTCAGTCTTATTTCATCATTCTTAATATCGTTAAAGACCAACTTGTATCTTAAACAAATACTATCTCAATATGTACAAAGCTTGTTATTTATTCAGCATTTTTTGCCGTTCTTCATTATATAGACT > CP000730/2041157‑2041417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |