Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,315 G→A 58.8% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3150GA58.8% 182.3 / inf 600Y79Y (TAC→TATUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base G (105/133);  new base A (149/195);  total (266/334)
Fisher's exact test for biased strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

Only 100 of 602 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATT  >  CP000730/36175‑36453
                                                                                                                                            |                                                                                                                                          
ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                                                            <  2:45866/141‑1 (MQ=32)
 tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                                                                           <  2:111208/141‑1 (MQ=32)
          ttttAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                  <  1:323994/140‑1 (MQ=17)
           tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatc                                                                                                                                 <  2:314672/141‑1 (MQ=12)
              tagtagaCGTTTGACAACCGCATATGCTGAACGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAACGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                              <  2:189981/137‑1 (MQ=11)
                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTTTCATCAATAc                                                                                                                            >  1:381809/1‑141 (MQ=17)
                 gagaCTTTTGATAATCCCATATGCTGAACGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACg                                                                                                                           <  2:159049/141‑1 (MQ=11)
                   gaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGcc                                                                                                                         >  2:204661/1‑141 (MQ=17)
                          gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCACTGGCACGATCTAACTGGTACCAATTTCCTTTTATTTTGATATAAGGCTCATGAATACGACGTTTGta                                                                                                                  >  1:247860/1‑141 (MQ=11)
                           ctAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                 <  1:202010/140‑1 (MQ=17)
                            aaaaCGCATATGCTGAAGGATCATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATCTGtgat                                                                                                                <  2:4165/140‑1 (MQ=11)
                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATCAGCCATTTGtaata                                                                                                               >  1:124409/1‑141 (MQ=255)
                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCTTCAATGGCACGATATAAATAGCTCCATTTTCTTTTTATTTTGATGTACGTCTTTTCAATACGTAATTTGtaata                                                                                                               >  1:337469/1‑141 (MQ=11)
                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                                                               <  2:207093/141‑1 (MQ=32)
                              atcGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                                                              <  2:297681/139‑1 (MQ=21)
                              aaCGCATATGCGGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                                                              <  2:229241/141‑1 (MQ=18)
                                cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATACGCCATTTGtattaatc                                                                                                            >  1:182622/1‑139 (MQ=16)
                                 gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCt                                                                                                           <  2:316548/141‑1 (MQ=255)
                                  cATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTGTCATCAATACTCCATTTGTAATAAGCtt                                                                                                          >  1:167893/1‑141 (MQ=255)
                                   atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttt                                                                                                         <  1:263478/141‑1 (MQ=35)
                                      tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTATCCTTTTGTTTTGATTTACGGCTCATAAAAACGACATTTTTAGAAAGCtttttt                                                                                                      >  1:348819/1‑141 (MQ=11)
                                       gCTGAATGAAAAACTCGCTGCTTACGCCTCAAATTAACTGATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                                                                     <  1:285663/141‑1 (MQ=11)
                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                    <  2:220507/141‑1 (MQ=38)
                                         tGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                                                                   <  2:54609/141‑1 (MQ=255)
                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtt                                                                                                <  1:149812/141‑1 (MQ=40)
                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCACAAATCTGAATTTATTTTGATGTACGTCTCATTAATACGCAAATAGCAATAAGCTTTTTTACGcct                                                                                                >  1:90902/1‑139 (MQ=11)
                                                ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCtttttt                                                                                            >  2:12115/1‑140 (MQ=255)
                                                ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTCTATTTTGATATACGTCTCATCAATACGCCATTTGTAATGAGCTTTTTTATGCTTTttc                                                                                            <  1:237573/141‑1 (MQ=255)
                                                ttACCTCGCTGCCTACTCAACCAAATACCTAATTTACGTCCCTCTGCACCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                                                            <  1:6487/141‑1 (MQ=14)
                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttttt                                                                                           >  1:359443/1‑139 (MQ=39)
                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                          >  1:243835/1‑141 (MQ=255)
                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                          <  2:175185/141‑1 (MQ=40)
                                                    ttcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                        <  1:48450/140‑1 (MQ=255)
                                                         tGCTGACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTTTTCCAAGtt                                                                                   >  1:269305/1‑141 (MQ=14)
                                                              aCGCAACCATATATCTAATTTATGTCCCTGCGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTCATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                                                              <  2:369728/141‑1 (MQ=14)
                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGTAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                             <  2:248132/141‑2 (MQ=255)
                                                               cGCAAACAAAAACCTAATGCATGTCCCTCTGTATCAATGGCACGATATAATTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                             <  1:372034/141‑2 (MQ=14)
                                                                 cAACCAAATATCTAATGTATGTCTCCTTGTATCAATGGCACGATATAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                           <  2:32706/141‑1 (MQ=11)
                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAttt                                                                       >  2:344371/1‑141 (MQ=255)
                                                                      aaaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                      <  1:306003/141‑1 (MQ=25)
                                                                         tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                   <  1:119774/141‑1 (MQ=255)
                                                                             tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATATACGTCTCATCAATACGACATTTTTAATAAGATTTTTTATGCTTTTTATTACAAATTTTATTTAAAATTGAGGAata                                                               >  1:105474/1‑141 (MQ=11)
                                                                             tAATGTAAGTCCCTCTGTAACAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                               <  1:40599/141‑1 (MQ=11)
                                                                              aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                              <  2:291764/141‑1 (MQ=255)
                                                                                tgtatgtCCCTCAGTATCAATGGCACGATATAAAAAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTCATAAGCTTTTTTCTGCTTTTTCTTCCACATCTGATCTCAAATTGGCGCACATTc                                                            >  2:91112/1‑141 (MQ=11)
                                                                                   atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                         <  1:223426/141‑1 (MQ=255)
                                                                                    tttCCCTCTTCATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTGATTTTCATATACGTCTCAGCAATACGCCATTTGTAATAAGCTTCTTTATGCTTTTTCTTCCAAATTGGTTATGAAATTGGGGCATATTTTTgt                                                        <  2:19976/139‑2 (MQ=11)
                                                                                    tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTACGCCATTTGTAATAATCTTTATTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                        >  1:206846/1‑141 (MQ=11)
                                                                                     gtCCCTTTGGATCAATGGCCCGATATAAATAGCCCCCTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                       <  2:237472/141‑1 (MQ=11)
                                                                                      tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                      <  2:384159/141‑1 (MQ=255)
                                                                                       cccTCTGTATCAATGCCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTAGTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTCTATATAAAATTGAGGCATTATCTTGAAcc                                                     >  2:225426/1‑141 (MQ=11)
                                                                                        ccTCTGTATCAATAGCACGATATAAATAGCTCCATTTTCCTTATATTTTGATATATCTCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCAACTTTCATATAAAATTGTGGCACATTCttcgagcc                                                    >  2:380664/1‑135 (MQ=11)
                                                                                             ttttctaTGCCACGATTCAAACCGCTCCAGCTTCCTTTTATTTTGCTTTCCGCCTCATAAATACGCCATTTGTAAGAAGCTTTTTTATCCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCGAACgg                                               <  2:186245/135‑1 (MQ=2)
                                                                                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                               <  2:303693/139‑1 (MQ=255)
                                                                                             gtaTCAATGGCACCAATCAAATAACTTCATTTTCCTTTTATTTTGATATACGTCTCACCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                               <  1:331559/139‑1 (MQ=11)
                                                                                              cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTCGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                              <  1:258814/141‑1 (MQ=18)
                                                                                                taaaTGGCACGATATAAATAGCTCCCGTTGCCTTTTATTTTGATGTACGTCGCATCAATACGCCATTTGTAATAACCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                            <  1:192712/139‑1 (MQ=11)
                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGAAACATATTCTTGAACCCAACGGTAg                                            <  1:291106/141‑1 (MQ=255)
                                                                                                  aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGat                                          >  2:39386/1‑140 (MQ=255)
                                                                                                   aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAACTTGATATAAAATTGGGGCATATTCTTGAATCCAACGGTAGAcc                                         >  2:8168/1‑141 (MQ=255)
                                                                                                    tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTTTCTTGAACCCAAAGGTAGATCg                                        >  1:15908/1‑141 (MQ=11)
                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCACTTTTGTTATAAAATTGGGGCGTATTTTTGAACGCAAGGGTaaatctt                                       >  2:359529/1‑135 (MQ=11)
                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                       <  2:256264/141‑1 (MQ=11)
                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                       >  1:177543/1‑141 (MQ=255)
                                                                                                     ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                       <  1:367330/141‑1 (MQ=255)
                                                                                                       cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                     >  1:311425/1‑141 (MQ=21)
                                                                                                       cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                     >  1:320053/1‑141 (MQ=25)
                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAACCCAACGGTAGACCGTTGa                                    <  1:252381/141‑1 (MQ=21)
                                                                                                         cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                   <  1:278430/141‑1 (MQ=255)
                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                 >  1:101031/1‑141 (MQ=255)
                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                              >  2:149075/1‑141 (MQ=255)
                                                                                                               aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACGCAACGGTAGACCGTTGAAtgatga                             <  2:319593/141‑1 (MQ=255)
                                                                                                                aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                            >  1:137066/1‑141 (MQ=255)
                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAATCCAACGGTAGACCGTTGAATGATGAAc                           >  2:140067/1‑141 (MQ=21)
                                                                                                                   aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                         >  2:274433/1‑141 (MQ=255)
                                                                                                                    gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                        <  1:5898/141‑1 (MQ=255)
                                                                                                                      tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                      >  1:228854/1‑141 (MQ=21)
                                                                                                                      tCCATTTTCCTTTTATTTTGATATGCGTCTGATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                      <  2:96032/141‑1 (MQ=11)
                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAGTAACGTTTAcacc                  <  2:282387/141‑1 (MQ=11)
                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                  <  1:255140/141‑1 (MQ=255)
                                                                                                                           tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                 <  1:294590/141‑1 (MQ=255)
                                                                                                                            ttCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                <  2:74440/141‑1 (MQ=255)
                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGACTGATGTACGTTTAaaccac                >  2:117035/1‑141 (MQ=11)
                                                                                                                             tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGATCGTTTACACCACg               >  2:213127/1‑141 (MQ=18)
                                                                                                                             tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg               >  2:253186/1‑141 (MQ=255)
                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt              >  2:12933/1‑141 (MQ=25)
                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt             >  1:38699/1‑141 (MQ=21)
                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt             >  1:144813/1‑141 (MQ=255)
                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACACCGtt             >  1:79017/1‑141 (MQ=11)
                                                                                                                                ttttATTTTGATGTACGTCTCAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc            <  2:6710/141‑1 (MQ=25)
                                                                                                                                    aTTTTGATTTACGTCTTCTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTGGAACCCAACGTTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt        <  1:153565/141‑1 (MQ=11)
                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt        <  2:101031/141‑1 (MQ=255)
                                                                                                                                     ttttGATGTCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTATACCTCGTTCCCTTa       <  2:268077/141‑1 (MQ=255)
                                                                                                                                     ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa       <  2:364701/141‑1 (MQ=11)
                                                                                                                                       ttGATGTACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat     <  2:332956/141‑1 (MQ=21)
                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat     <  2:60942/141‑1 (MQ=11)
                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat     <  1:112734/141‑1 (MQ=11)
                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGGATGATGAACGTTTACACCACGTTCCCTTAatat   <  2:310135/141‑1 (MQ=18)
                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat   <  1:218740/141‑1 (MQ=21)
                                                                                                                                         gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat   >  1:356858/1‑141 (MQ=25)
                                                                                                                                            |                                                                                                                                          
TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATT  >  CP000730/36175‑36453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: