BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	91.1%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	91.1%	958.4 / 80.9	485	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (21/23); new base T (214/226); total (236/249)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 487 total aligned reads displayed.

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATC  >  CP000730/36238‑36518

  cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTACATTTTCTTTTTATTTTGATATTCGTCT‑GATCAATACGCCATATGTAATAACCTTTTGTATACTTTTGCTTCCAAATTTGTTATaa                                                                                                                                            >  1:210701/1‑141 (MQ=11)

      cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAttt                                                                                                                                        >  2:344371/1‑141 (MQ=255)

        aaTATCTTATGTGTGTCCCTCTGCATCAATAGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTCTATGCTTTTTTTTCCAAATTTGATATAAAATTgg                                                                                                                                      <  2:371315/141‑1 (MQ=11)

        aaTATCTAATGTATGTCCTACGTTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                      >  2:382390/1‑141 (MQ=11)

        aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATAAAATTgg                                                                                                                                      <  1:294138/141‑1 (MQ=11)

        aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                      <  2:254456/141‑1 (MQ=37)

        aaTATCTAATGTATGTCCCTCTGCAACAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                      <  1:247469/141‑1 (MQ=255)

          tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                    <  2:248554/141‑1 (MQ=255)

          tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                    <  1:122114/141‑1 (MQ=255)

          tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                    <  1:353131/141‑1 (MQ=255)

          tagcTAATGTATGTCCCTCGGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CAGCAATACGCCATTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                    <  2:361800/138‑1 (MQ=11)

            tCTAATGTATGTCTCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATTTACGTCT‑CATCAATTCGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAATTTTGATATGAAATTTGGTCa                                                                                                                                  >  1:106055/1‑141 (MQ=11)

             ttAATTTATGTCCCTCGTTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                 <  2:302556/140‑1 (MQ=11)

             cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                 <  1:258232/141‑1 (MQ=37)

              tAATGTAAGTCCCTCTGTAACAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                                                                                <  1:40599/141‑1 (MQ=11)

                 tgtatgtCCCTCAGTATCAATGGCACGATATAAAAAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTCATAAGCTTTTTTCTGCTTTTTCTTCCACATCTGATCTCAAATTGGCGCACATTc                                                                                                                             >  2:91112/1‑141 (MQ=11)

                  ttatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                            <  2:356395/140‑1 (MQ=255)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                          >  1:375992/1‑141 (MQ=255)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                          <  1:345877/141‑1 (MQ=255)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATCTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGAGGCATATTCTTg                                                                                                                          <  1:280028/141‑1 (MQ=11)

                    atgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                          <  1:36669/141‑1 (MQ=25)

                     tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTGGATATAAAATTGGGGCATattattta                                                                                                                         >  2:46329/1‑139 (MQ=255)

                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACTTCT‑CATCAATACGCCATTTTTAATAAGCTTTTTTCTGGTTTTTCTTCCCAATTTGATATAAAATTGGGGCAGATTCCTGAAc                                                                                                                       >  1:10406/1‑141 (MQ=11)

                         ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTCTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGACATATTCTTGAAccc                                                                                                                     >  2:41352/1‑141 (MQ=11)

                               tatccagggtaCGGTATATATTGTTCCTTTTTTCTTTTATTGTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTGAGGGTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                               <  1:11881/131‑1 (MQ=11)

                                aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTa                                                                                                              >  1:356877/1‑141 (MQ=25)

                                 tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             <  1:328972/141‑1 (MQ=19)

                                  cactGGCACGAGATAAATAGCTCCATTTTCCTTTTGTTTTTATGTACGTCA‑CACCACTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                                                                                            <  2:210592/138‑1 (MQ=14)

                                  cAATGGCACGATATAAATAACTCAATCAGCGTTTTATTTTGATATACGTCT‑ACTCAATACGCCATCTGCAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATCCTTGAACCCAACGGTAGa                                                                                                            >  2:280405/1‑141 (MQ=11)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGaac                                                                                                          >  1:312671/1‑139 (MQ=39)

                                    aTGGCACGACATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                                                                                          <  1:7470/141‑3 (MQ=255)

                                      ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAgctcgt                                                                                                        >  2:224461/1‑136 (MQ=255)

                                      ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                        >  1:177543/1‑141 (MQ=255)

                                        cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                      >  1:320053/1‑141 (MQ=25)

                                        agcGAGATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                      >  2:199589/3‑141 (MQ=19)

                                         aCGATATAAATAGCTCTATCTTCCTTTTTGTGTTATTTCCGTATACACCCA‑ATACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                     <  1:285871/141‑1 (MQ=2)

                                             tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                 >  1:224680/1‑141 (MQ=21)

                                             tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                 >  1:165131/1‑141 (MQ=255)

                                               taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                               >  2:149075/1‑141 (MQ=255)

                                                aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                              >  2:59547/1‑141 (MQ=255)

                                                 aaTAGCTCCATTTTACTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                             <  1:305742/141‑1 (MQ=18)

                                                  aTAGCTTCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                            <  2:233277/141‑1 (MQ=21)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                                                                                            >  1:244702/1‑141 (MQ=255)

                                                   tAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAACGGTAGATCGTTGAatgatgcaac                                                                                           >  2:96458/1‑137 (MQ=11)

                                                   tAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCAAATTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTAGATATAAAATTGGTGCTTTTTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                           >  2:132482/1‑141 (MQ=11)

                                                    aGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                          >  2:274433/1‑141 (MQ=255)

                                                      cTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                        <  2:125199/141‑1 (MQ=255)

                                                       cccatctcacTTTTACTTTGATATACGTCT‑CAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                       <  2:158572/132‑1 (MQ=11)

                                                        ccATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                                                                                      <  1:231311/141‑1 (MQ=255)

                                                        ccATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATAGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                                                                                      <  1:262326/141‑1 (MQ=255)

                                                         cATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                                     <  2:387513/141‑1 (MQ=21)

                                                         cATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                     <  2:188343/141‑1 (MQ=255)

                                                          aTTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGACCGTTGAATGATTAATGTTTacac                                                                                    >  2:263361/1‑141 (MQ=11)

                                                          aTTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                    <  2:21959/141‑1 (MQ=40)

                                                          aTTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                    <  2:204663/141‑1 (MQ=255)

                                                           ttttCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAGTAAACTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAGAACATATATTTGAACACAACGGTAGATCTGTGAATGATGCACGTTTacagc                                                                                   >  2:10765/1‑139 (MQ=11)

                                                           ttttCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                   >  2:365878/1‑141 (MQ=255)

                                                            tttCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                  <  1:148528/141‑1 (MQ=18)

                                                            tttCCTTATATTTTGATTCACGTCT‑TATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGTTATAAAATTGGGCCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                  <  1:157590/141‑1 (MQ=11)

                                                            tttCATTTTATTTTGATTTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATATCGTTGAATGATGAACGTTTAcacca                                                                                  <  2:318938/141‑1 (MQ=11)

                                                              tCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                                >  1:251940/1‑141 (MQ=40)

                                                                cTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                              >  1:38699/1‑141 (MQ=21)

                                                                cTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                              >  1:63536/1‑141 (MQ=255)

                                                                    tattgctatttACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                          <  1:100457/131‑1 (MQ=11)

                                                                     tttttGATGTACGTCT‑CATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                         <  2:67579/140‑1 (MQ=21)

                                                                      ttttGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                        >  2:2944/1‑141 (MQ=21)

                                                                       tttGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                       <  2:227909/141‑1 (MQ=11)

                                                                        ttGATGTACTTCT‑CATCAACACGCCATTTGTAATAAGCCTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                      <  2:323555/141‑1 (MQ=255)

                                                                          gatatACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                    >  1:142221/5‑141 (MQ=11)

                                                                          gatatACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                    >  1:219436/5‑141 (MQ=11)

                                                                          gATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGGATGATGAACGTTTACACCACGTTCCCTTAatat                                                                    <  2:310135/141‑1 (MQ=18)

                                                                          gATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACAACGTTGCCATAatat                                                                    >  1:201638/1‑141 (MQ=11)

                                                                           aTGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGGATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATGtt                                                                   >  2:285718/1‑141 (MQ=255)

                                                                              tACTTCT‑CATCAATACCCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                <  1:124268/141‑1 (MQ=255)

                                                                               aCGTCT‑CATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                               <  1:96348/141‑1 (MQ=255)

                                                                                 gTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                             <  1:253186/141‑1 (MQ=255)

                                                                                    t‑catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                          <  1:267404/141‑1 (MQ=18)

                                                                                      catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCCGatatat                                                         >  1:296951/1‑141 (MQ=255)

                                                                                      catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                         >  2:61036/1‑141 (MQ=255)

                                                                                        tcaACACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                       <  2:116477/141‑1 (MQ=11)

                                                                                         caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATCTCAc                                                      >  1:58797/1‑141 (MQ=255)

                                                                                          aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                     >  1:194129/1‑141 (MQ=255)

                                                                                           aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGTCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                    <  2:239415/141‑1 (MQ=255)

                                                                                            tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAATATTGGGGAATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAATGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                   <  2:74690/141‑1 (MQ=11)

                                                                                             aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                  <  2:8373/141‑1 (MQ=255)

                                                                                              cgcaaatggTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                 <  2:213233/133‑2 (MQ=11)

                                                                                              cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                 >  2:328996/1‑141 (MQ=21)

                                                                                               gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                <  1:84372/141‑3 (MQ=255)

                                                                                               gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                <  1:189168/141‑3 (MQ=255)

                                                                                                ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                               >  1:129477/1‑141 (MQ=255)

                                                                                                ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                               >  1:155344/1‑141 (MQ=21)

                                                                                                ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAGGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                               <  1:371416/141‑1 (MQ=18)

                                                                                                   tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                            <  2:63536/141‑1 (MQ=255)

                                                                                                       taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                        >  1:13931/1‑139 (MQ=11)

                                                                                                       taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                        <  1:49628/141‑3 (MQ=11)

                                                                                                       taataaGCTTTTTTACGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCAGATGTATCACGATAGCTCAATgca                                        <  1:240579/141‑3 (MQ=11)

                                                                                                        aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGCTACATCACGATAACTCAATgcat                                       <  2:182622/141‑4 (MQ=2)

                                                                                                          taaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTatat                                     <  2:336082/141‑1 (MQ=40)

                                                                                                                 ttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAt                              <  1:337162/141‑1 (MQ=35)

                                                                                                                  ttttTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGCACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                             <  1:152762/141‑1 (MQ=25)

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATC  >  CP000730/36238‑36518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`