BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,413	C→T	54.6%	V47I (GTC→ATC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,413	0	C	T	54.6%	26.6 / inf	329	V47I (GTC→ATC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (69/79); new base T (90/89); total (161/168)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01

Only 100 of 337 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATA  >  CP000730/36273‑36552

 aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCT‑CATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                                                            >  1:391034/1‑141 (MQ=255)

 aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAACTTGATATAAAATTGGGGCATATTCTTGAATCCAACGGTAGAcc                                                                                                                                            >  2:8168/1‑141 (MQ=255)

  tggcctgATATAAATAGCACCTTTTTCTTTTTATTTTTATATACCTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                                           <  1:44078/135‑1 (MQ=11)

   ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                                                          <  2:256264/141‑1 (MQ=11)

     cACGATATAAATAGCACCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAGCGTTg                                                                                                                                        >  2:321779/1‑141 (MQ=255)

      aCGATATAATTTGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                       <  2:116290/141‑1 (MQ=255)

      aCGATATAAATAGCTCTATCTTCCTTTTTGTGTTATTTCCGTATACACCCA‑ATACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                                                       <  1:285871/141‑1 (MQ=2)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAACCCAACGGTAGACCGTTGa                                                                                                                                       <  1:252381/141‑1 (MQ=21)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACTGTAGATCGTTGa                                                                                                                                       >  1:105820/1‑141 (MQ=255)

       cGAGATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                      <  1:152031/141‑1 (MQ=11)

         atatAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                    <  1:200334/141‑1 (MQ=255)

          tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                                                   >  1:224680/1‑141 (MQ=21)

          tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                                                   <  2:20553/141‑1 (MQ=40)

           ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                                                                                                                  <  1:375408/141‑1 (MQ=21)

            taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                                                                 >  1:21959/1‑141 (MQ=40)

              aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                               >  1:324335/1‑141 (MQ=255)

              aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                               >  1:137066/1‑141 (MQ=255)

               aTAGCTTCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                                                              <  2:233277/141‑1 (MQ=21)

               aTAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                                                                                                                              <  1:316878/141‑1 (MQ=255)

                tAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                                                             <  1:68482/141‑1 (MQ=255)

                tAGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAACGGTAGATCGTTGAatgatgcaac                                                                                                                             >  2:96458/1‑137 (MQ=11)

                tAGCTCCATTTTCCTTTTATTGTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                                                             <  1:246202/141‑1 (MQ=255)

                 aGCTCCATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGt                                                                                                                            >  2:289701/1‑141 (MQ=255)

                    tCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                                                                                                                         >  1:228854/1‑141 (MQ=21)

                    cccatctcacTTTTACTTTGATATACGTCT‑CAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                                                         <  2:158572/132‑1 (MQ=11)

                     ccATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATAGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                                                                                                                        <  1:262326/141‑1 (MQ=255)

                      cgtgtcgtttttATTTTGATATTCGTCG‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                       <  2:94749/133‑1 (MQ=11)

                      cATTTTCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                       <  2:188343/141‑1 (MQ=255)

                        ttttCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                     >  2:211901/1‑141 (MQ=18)

                        ttttCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                     <  2:31089/141‑1 (MQ=255)

                        ttttCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                     >  2:365878/1‑141 (MQ=255)

                         tttCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                    <  2:340104/141‑1 (MQ=21)

                         tttCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                    <  1:148528/141‑1 (MQ=18)

                         tttCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                    <  1:202661/141‑1 (MQ=255)

                         tttCCTTATATTTTGATTCACGTCT‑TATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGTTATAAAATTGGGCCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                    <  1:157590/141‑1 (MQ=11)

                         tttCATTTTATTTTGATTTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATATCGTTGAATGATGAACGTTTAcacca                                                                                                                    <  2:318938/141‑1 (MQ=11)

                          ttCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                                   >  1:54869/1‑141 (MQ=255)

                          ttCCTTGTATTTTTATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCccccac                                                                                                                   >  2:183477/1‑141 (MQ=11)

                           tCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                                                                  >  1:251940/1‑141 (MQ=40)

                           tCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                                  >  1:27522/1‑141 (MQ=255)

                           tCCTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                                  <  2:370367/141‑1 (MQ=255)

                            ccTTTTATTTTGATATACGTCT‑CATCAATACGCTATTTGTAATAAGCTTTTTTCTACTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                                 <  2:217492/141‑1 (MQ=11)

                            ccTTTTATTTCGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                                                      >  2:281528/1‑136 (MQ=11)

                            ccTTTCATTTTGATAGACGTCT‑CATCAATACGCCATTTGTAATAAGCTGTTTTATGCTTTTTCTTCCAAGTTTGATATAAAATTGCGGCATATTCTTTAACCCGACGGTAACTCGTTGAATCTTGAACGtttgaccaacgt                                                                                                                 >  2:129224/1‑132 (MQ=11)

                             cTTTTATTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                >  1:253752/1‑141 (MQ=255)

                              ttttATTTTGATGTACGTCT‑CAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                               <  2:6710/141‑1 (MQ=25)

                               tttATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGGAGGCCGTTGAATGAGGAACGATTACACCACGTTcc                                                                                                              >  1:386330/1‑141 (MQ=11)

                                 tattgctatttACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                            <  1:100457/131‑1 (MQ=11)

                                  aTTTTGATTTACGTCT‑TCTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTGGAACCCAACGTTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                           <  1:153565/141‑1 (MQ=11)

                                  aTTTTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                           <  2:101031/141‑1 (MQ=255)

                                  aTTGTGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                           <  2:391698/141‑1 (MQ=11)

                                   ttttGATGTCCGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTATACCTCGTTCCCTTa                                                                                                          <  2:268077/141‑1 (MQ=255)

                                   ttttGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                          <  2:364701/141‑1 (MQ=11)

                                    tttGTTGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCCCACAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                         <  1:308476/141‑1 (MQ=11)

                                    tttGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                         <  2:227909/141‑1 (MQ=11)

                                      tGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTGTACACCACGTTCTCTTAata                                                                                                       >  1:292213/1‑141 (MQ=11)

                                      tGATATACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGACTATTCTTGAACCCAACGGCAGATCGTTGAATGATGAACGTTCACAAGACGTTCTATTAata                                                                                                       >  1:347227/1‑141 (MQ=11)

                                       gatatACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACAACACGTTCTATTAatat                                                                                                      >  1:81145/5‑141 (MQ=11)

                                       gATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                      >  1:356858/1‑141 (MQ=25)

                                        atatACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                                                     <  2:62373/138‑1 (MQ=11)

                                          atACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                                                   <  2:243835/140‑1 (MQ=255)

                                           tACTTCT‑CATCAATACCCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                  <  1:124268/141‑1 (MQ=255)

                                           tACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                  >  2:71397/1‑141 (MQ=255)

                                            aCGTCT‑CATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                                                 <  1:96348/141‑1 (MQ=255)

                                             cGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                  <  2:124364/139‑1 (MQ=21)

                                               tct‑cATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGata                                                                                              >  1:90267/1‑141 (MQ=18)

                                                ct‑cATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                                                                             <  1:140431/141‑1 (MQ=18)

                                                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCCGatatat                                                                                           >  1:296951/1‑141 (MQ=255)

                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                       >  2:350619/1‑141 (MQ=255)

                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                                                       >  1:155771/1‑141 (MQ=40)

                                                        aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTGAGATATATAACGa                                                                                      >  2:284944/1‑141 (MQ=11)

                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAATATTGGGGAATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAATGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                     <  2:74690/141‑1 (MQ=11)

                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                     >  1:119130/1‑141 (MQ=16)

                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGATATATCCCGAt                                                                                     >  1:103847/1‑141 (MQ=11)

                                                         tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTAAAGTATATCGCGAt                                                                                     >  1:142335/1‑141 (MQ=11)

                                                           cgcaaatggTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                   <  2:213233/133‑2 (MQ=11)

                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATGTTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTACTATTTCATATATATCACGATag                                                                                   >  1:126285/1‑140 (MQ=11)

                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATAACGATag                                                                                   >  1:17695/1‑140 (MQ=255)

                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                   >  1:351822/1‑141 (MQ=25)

                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                   >  2:328996/1‑141 (MQ=21)

                                                            gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                  <  1:189168/141‑3 (MQ=255)

                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAGGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                 <  1:371416/141‑1 (MQ=18)

                                                               aTTTGTAAAAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                               <  2:25019/141‑1 (MQ=11)

                                                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                              >  1:243355/1‑141 (MQ=255)

                                                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTCGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                            <  2:200309/141‑1 (MQ=255)

                                                                   gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                           <  1:367136/141‑2 (MQ=11)

                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCCCGATAACTCAATgca                                                                          >  2:386274/1‑139 (MQ=12)

                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGGTCCCTTAATATTTCAAAAATAAAACGATAACTCAATgca                                                                          >  2:142394/1‑139 (MQ=11)

                                                                    taataaGCTTTTTTACGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATTATGAACGTTTACACCACGTTCTCTTAATATTTCAGATGTATCACGATAGCTCAATgca                                                                          <  1:240579/141‑3 (MQ=11)

                                                                       taaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTatat                                                                       <  2:336082/141‑1 (MQ=40)

                                                                          gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                    >  1:60057/1‑141 (MQ=38)

                                                                                  aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                            >  1:237534/1‑141 (MQ=35)

                                                                                            ttcCAAATTGGAGACAAAATTGGGGCCTATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                  <  2:168775/141‑1 (MQ=255)

                                                                                                         aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                            <  2:301000/134‑1 (MQ=34)

                                                                                                         aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                            >  1:301000/1‑134 (MQ=33)

                                                                                                          cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATc                                    >  2:149589/1‑141 (MQ=33)

                                                                                                                tGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGtt                              <  1:174269/141‑1 (MQ=12)

                                                                                                                   ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                           >  2:274496/1‑141 (MQ=32)

                                                                                                                        atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                      >  2:224855/1‑141 (MQ=32)

                                                                                                                               aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt               <  2:60057/141‑1 (MQ=32)

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCT‑CATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATA  >  CP000730/36273‑36552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`