Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,378 C→T 100% D586N (GAT→AAT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,3780CT100.0% 107.0 / ‑6.5 38D586N (GAT→AAT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/15);  minor base G (1/0);  total (23/15)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATT  >  CP000730/39242‑39513
                                                                                                                                        |                                                                                                                                       
aTAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTACGTTTGCATAGGATTTTTAATTAttttct                                                                                                                                     >  2:159266/1‑141 (MQ=255)
   gCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCtttt                                                                                                                                  >  1:195549/1‑140 (MQ=255)
     catcatcatGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCGCCAGGTTACATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATAGTCTTAAtg                                                                                                                                >  2:107116/1‑141 (MQ=255)
                ttGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAATTAGTTTCTTTATGTGTtttattta                                                                                                                     <  2:122649/141‑1 (MQ=255)
                ttGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTtttattta                                                                                                                     <  2:327638/141‑1 (MQ=255)
                   gatttccgttctCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGa                                                                                                                  <  2:275704/130‑1 (MQ=255)
                      ttgcTTTATCATATGATATTAACCACCCTATTTGTCTTCGATGTTCTCCTTGTTTCGTTTTGAGTTCTGCAGTACCTGTTTTGCCAATTTAGTTTGCATAAGATCTATTAATATTTTCTTTATGTGTTTTATTTACGACtt                                                                                                               <  2:51890/138‑1 (MQ=255)
                          tgtATCATATGGTATAAACCACCCAATTTGTCTGCCAGTTGCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACttgttg                                                                                                           <  2:371477/139‑1 (MQ=255)
                           ttATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGc                                                                                                          >  2:371957/1‑141 (MQ=255)
                                 tatGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCa                                                                                                    >  2:311125/1‑141 (MQ=255)
                                             caccCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGGCTTGTTGCATACCATCAGTTAATAGa                                                                                        >  1:22347/1‑141 (MQ=255)
                                                  aaTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATGTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGat                                                                                   >  1:348286/1‑141 (MQ=255)
                                                   aTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGAACACGAGCAGTTCATAGATTGata                                                                                  >  2:276184/1‑141 (MQ=255)
                                                     ttGTCTGCCAGTTTCTCCTTGTCTCATCTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTATTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtt                                                                                >  2:333703/1‑141 (MQ=255)
                                                        tCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTTCGACTTGTTGCATACCATCAGTTAATAGATGTATAttttt                                                                             >  2:124892/1‑140 (MQ=255)
                                                            ccAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTg                                                                         <  2:8388/141‑1 (MQ=255)
                                                               gTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGGTTGCATAAGATGTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTTATAGATTGATATTTTCTTTGGaa                                                                      >  2:966/1‑141 (MQ=255)
                                                                 ttCTCCTTGTTTCAATTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaat                                                                    >  1:374647/1‑141 (MQ=255)
                                                                   ctcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaataa                                                                  >  2:227857/1‑141 (MQ=255)
                                                                   ctcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAaataa                                                                  <  2:142139/141‑1 (MQ=255)
                                                                    tcCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCTGTTAATAGATTGTTTTTTTCTTTGGAaataat                                                                 >  1:72653/1‑141 (MQ=255)
                                                                               ttttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAATATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTcc                                                      >  2:210936/1‑141 (MQ=255)
                                                                               ttttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCCGTTAATAGATTGATATTTTCTTTGGTAATAATATTTTTCTTcc                                                      >  2:192692/1‑141 (MQ=255)
                                                                               tcttGCGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATACGATCTATAAATATTTTCTTTATGTGTTTTATTTACGCCTTGTTGCATACGATCAGTTAATAGATTGATATTTTCTTTTGAAATAATCTTTTTCTTcc                                                      >  2:8581/3‑141 (MQ=255)
                                                                                     gTTCTTCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAACTAATATTTTTCTTCCAAACtt                                                <  2:142979/141‑1 (MQ=255)
                                                                                       tCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGCTAATAGATTGCTATTTTCTTTGGAAATAAAATTTTTCTTcccacctgtg                                              >  1:52409/1‑133 (MQ=255)
                                                                                        cTGCAGTACCGGATTGGCCAATTAAGTTTGCATGAGACCTATTAATATTTGCGTTATGTGTTTTATTTACGACGTGTTGCTTACCATCAGTGAACAGCTTGATATTTTCTTTGGAAATAATATTTTATGTCCAAAGttgct                                             >  2:90418/1‑138 (MQ=255)
                                                                                         tGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGtt                                            <  2:186975/141‑1 (MQ=255)
                                                                                          gCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACtttcatc                                           <  2:192116/141‑5 (MQ=255)
                                                                                           cAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATTTTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTGCTTTGGAAATAATATTTTTCTTCCAAACTTTGtttt                                          <  1:276184/141‑1 (MQ=255)
                                                                                                ccGGATTTGCCAATTAAGTGTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCACACTTTGTTTTTCgtg                                     >  2:36036/1‑141 (MQ=255)
                                                                                                       tGCCAATTAAGTTTGCATAAGATCTATAAATATTTTTTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTta                              <  2:204308/141‑1 (MQ=255)
                                                                                                            aTTAAGTTTGCATAAGATCTATAAATATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAGTAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAg                         <  1:270932/141‑1 (MQ=255)
                                                                                                            aTTAAGTGTGCATAAGATCTATAAATATTTTCTTGATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAGTAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAg                         <  1:333878/141‑1 (MQ=255)
                                                                                                                   ttGCATACGCTATATAAATATTTTCTTTATGTGTTTTATTAAAGCCTCGTCCCTTATTATCGGTGAATAGATTGATATTTTCTTTGGAAAAAATATTTTTCTTCCAAACTTTTTTTTTCTTGTCTTTTATTTAGTgtgctg                  >  2:279256/1‑136 (MQ=255)
                                                                                                                                tttaaTATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGcc     <  2:348286/138‑1 (MQ=255)
                                                                                                                                  aaaTATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAGACTTTGTTTTTCGTGTCTGTTAATAAGTGAGGTGCGTTAATATTACCat   >  1:104821/1‑141 (MQ=255)
                                                                                                                                   aaTATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCatt  >  1:89969/1‑141 (MQ=255)
                                                                                                                                   aaTATTTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCatt  >  1:136285/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCATT  >  CP000730/39242‑39513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑