Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,185 G→A 28.0% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1850GA28.0% 123.4 / 46.8 79intergenic (+243/‑891)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (32/24);  new base A (12/10);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01

AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGG  >  CP000730/76047‑76323
                                                                                                                                          |                                                                                                                                          
aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                          >  2:50403/1‑141 (MQ=255)
aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                          <  1:68325/141‑1 (MQ=255)
      aCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACAAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAttt                                                                                                                                    >  1:312293/1‑141 (MQ=255)
       cTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                                   <  1:157030/141‑1 (MQ=255)
            atTGCTGCACCTGAAGCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaa                                                                                                                              <  1:379752/141‑1 (MQ=255)
            agtgctgaACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaa                                                                                                                              <  2:155766/139‑1 (MQ=255)
                 tgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                         >  2:160763/1‑141 (MQ=255)
                 tgcACCGGGATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                         <  2:80473/141‑1 (MQ=255)
                 cgcACCTCTATCACTTAGAACAGCTAACCAAAGTGTTATGAATGCAGTGAAGACTAACACAAATTCAATGAATTTAAGAATTATCGCAAAATATATTTTCTGTTGCAAAGTTTAATCTATAGTATAATTTTAACaaaaacg                                                                                                                         <  2:189052/140‑3 (MQ=25)
                                    aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                      >  1:341198/1‑141 (MQ=255)
                                        cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                                  >  2:344525/1‑141 (MQ=255)
                                         tAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAg                                                                                                 >  1:46888/1‑141 (MQ=255)
                                         tAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAg                                                                                                 >  2:115616/1‑141 (MQ=255)
                                           aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAAat                                                                                               >  2:215129/1‑141 (MQ=255)
                                                  tgtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACa                                                                                        <  1:160763/141‑1 (MQ=255)
                                                    ttAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAt                                                                                      >  1:39863/1‑141 (MQ=255)
                                                      aGTAATCCAGGGAATACTAACACAAAGGAAATTAATTTAATAATTATAGCAAAATATAGGGTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAatt                                                                                    >  1:41785/1‑141 (MQ=255)
                                                             cAGGGAATACTAACCCAAAGGCAATTAATTTAATAATTATAGCCAAATACAAGCTGTCTTCCGAGGTGGAAACGACAGTGTAATTTTAGTAACAAGGCGTCTTCTGTATGAACAATTTCGGATATAAACAGTTTAACAAgc                                                                             >  2:99070/1‑140 (MQ=255)
                                                                ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                          >  2:126700/1‑141 (MQ=255)
                                                                ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                          >  2:368676/1‑141 (MQ=255)
                                                                    tACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGCGTCTTCTGTATGAACTATTTCAGATATAAACAATTTACCAAGGATGTGAt                                                                      >  1:67893/1‑141 (MQ=255)
                                                                       tAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAc                                                                   >  1:141302/1‑141 (MQ=255)
                                                                       tAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAc                                                                   >  1:308825/1‑141 (MQ=255)
                                                                           acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                               <  1:81327/141‑1 (MQ=255)
                                                                                    attaattTAATAATTATAGCATCACTTAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGc                                                      >  2:382035/1‑141 (MQ=255)
                                                                                     ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                     <  1:248814/141‑1 (MQ=255)
                                                                                         tttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTAAAAATATAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                                 <  2:231905/141‑1 (MQ=255)
                                                                                            aataatTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCt                                              <  2:118451/141‑1 (MQ=255)
                                                                                                      gCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                    >  1:377949/1‑141 (MQ=255)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGGGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAAGTTATCACTATAGCCGTGGGCTACTATCTAAGATATGCAt                                   >  1:225426/9‑141 (MQ=11)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:111819/9‑141 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:228863/9‑141 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:42238/9‑141 (MQ=11)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:149706/9‑141 (MQ=11)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:238127/9‑141 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:96458/9‑141 (MQ=1)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                   >  1:321794/9‑141 (MQ=255)
                                                                                                        gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                                  <  1:267022/134‑1 (MQ=255)
                                                                                                        agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                                  <  1:376980/134‑1 (MQ=255)
                                                                                                         gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                 >  1:39386/7‑141 (MQ=0)
                                                                                                          ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                                <  1:777/136‑1 (MQ=2)
                                                                                                          ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                                <  1:364429/136‑1 (MQ=2)
                                                                                                          aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                <  1:96830/136‑1 (MQ=9)
                                                                                                          aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGAATTGa                                >  2:334899/6‑141 (MQ=1)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATTATATAATTTTAACAAAAAGGGGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                               <  1:167026/137‑1 (MQ=11)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                               <  2:29169/137‑1 (MQ=2)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                               <  2:118340/137‑1 (MQ=9)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAATAAAAATAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                               <  2:190896/137‑1 (MQ=11)
                                                                                                            tataGGTTCTGTTGCAAAGTTAAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                              <  1:201344/141‑1 (MQ=38)
                                                                                                            aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              <  2:316418/138‑2 (MQ=9)
                                                                                                             tgatgttatttttcAAATTTTAATATATAATAAAAATAAAAAAAAAATTAGTCTTCTGCATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGTCTACTATCTAAGATATGCATTGAGtt                             <  2:129872/128‑1 (MQ=2)
                                                                                                             ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTTTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTgggct                             >  1:297384/3‑137 (MQ=11)
                                                                                                             ggaGGTTATATTGCAAAGTTGAATTTATAAAAAAAATAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAttcacct                             <  2:273729/139‑6 (MQ=11)
                                                                                                             ataGGTTCGGTTGCAAAGTCGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAATAATTTAACAAGGATGTTATCTCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                             <  2:58167/141‑1 (MQ=255)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           >  1:95538/1‑141 (MQ=0)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           >  2:95372/1‑141 (MQ=11)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           <  2:298743/141‑1 (MQ=11)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                           >  1:57033/1‑141 (MQ=2)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                           >  2:393788/1‑141 (MQ=2)
                                                                                                                 gTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGCTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCg                         >  1:369734/1‑141 (MQ=25)
                                                                                                                 gTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACAATCTAAGATATGCATTGAGTTATCg                         >  2:88886/1‑141 (MQ=21)
                                                                                                                    cttttGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCGTTATAAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                      <  1:52469/138‑1 (MQ=11)
                                                                                                                      tatgaaaatctGAATCTATACTATAAGTAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                    <  2:228913/131‑1 (MQ=11)
                                                                                                                      gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                    <  1:87362/141‑1 (MQ=35)
                                                                                                                         gCAAAGTGGAATCTATAGTATAATGTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATGAACAATGTAACAAGGAGGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGctatat                 >  2:99595/1‑141 (MQ=11)
                                                                                                                          cAATGTTGAATCTATAATATAATTTAAACAAAAAGGAGGCTTCTGTATGAACGATTTCAGATACAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATc                <  1:27183/141‑1 (MQ=11)
                                                                                                                              gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa            <  2:311398/141‑1 (MQ=35)
                                                                                                                                   ctttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt       >  2:165131/4‑141 (MQ=2)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATTTCTGAAATAtt       >  1:118063/4‑141 (MQ=0)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt       >  1:180064/4‑141 (MQ=0)
                                                                                                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt       >  1:249888/4‑141 (MQ=0)
                                                                                                                                    tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTGAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTa      >  2:253422/3‑141 (MQ=9)
                                                                                                                                    tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa      >  1:49070/3‑141 (MQ=11)
                                                                                                                                    tttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa      >  2:255562/3‑141 (MQ=9)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa     <  2:228960/140‑1 (MQ=12)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa     <  2:322435/140‑1 (MQ=12)
                                                                                                                                     ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa     <  1:214322/140‑1 (MQ=12)
                                                                                                                                     ttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa     <  2:363759/140‑1 (MQ=17)
                                                                                                                                     ttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa     <  2:57554/140‑1 (MQ=17)
                                                                                                                                     cTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCCTTGAGTTATCGTGATATATCTGAAATATTaa     <  2:363036/141‑1 (MQ=17)
                                                                                                                                     cTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa     >  2:365147/1‑141 (MQ=17)
                                                                                                                                       ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGTCTACTATCTAAGATATGCATTTAGTTATCGTGATATATCTGAAATATTAAgg   <  2:190131/141‑1 (MQ=12)
                                                                                                                                       ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg   >  1:339372/1‑141 (MQ=17)
                                                                                                                                        taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg  >  1:42489/1‑141 (MQ=17)
                                                                                                                                        taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg  >  2:230055/1‑141 (MQ=17)
                                                                                                                                        taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGCTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg  >  2:230051/1‑141 (MQ=17)
                                                                                                                                        taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg  >  1:286325/1‑141 (MQ=32)
                                                                                                                                          |                                                                                                                                          
AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGG  >  CP000730/76047‑76323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: