Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,889 G→A 19.1% intergenic (+947/‑187) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,8890GA19.1% 68.9 / 7.2 35intergenic (+947/‑187)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (20/8);  new base A (4/3);  total (24/11)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAA  >  CP000730/76750‑77029
                                                                                                                                           |                                                                                                                                            
gTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCCAGTTAAGc                                                                                                                                             >  1:190859/1‑141 (MQ=17)
      aaaTATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAacac                                                                                                                                       <  1:115626/141‑1 (MQ=32)
            caatcaaGCAAAGAATACTTTAAAAGGTATTGAGTGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACAt                                                                                                                                 <  1:133890/135‑1 (MQ=0)
                acggCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATa                                                                                                                             >  2:19997/4‑141 (MQ=0)
                                    agggagtGAATGTATTTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAtt                                                                                                         <  2:46769/135‑1 (MQ=1)
                                           gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCCCGAAATTAGCATCATGCTAGCAGGTTAGGAGGACAGTGACATGCCAGATTGGTGGTTAGGTATGTTTTTTTAc                                                                                                  >  1:81609/4‑141 (MQ=1)
                                           gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAc                                                                                                  <  1:235921/138‑1 (MQ=1)
                                           gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAc                                                                                                  <  1:324005/138‑1 (MQ=1)
                                           gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttact                                                                                                  >  2:136963/4‑138 (MQ=1)
                                           gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATATACGGATATTCGCCATGCCACGAAATTAGCATCACGCTAGCAAGTTAAACGAACACTGACAAGATATATTAGTGATTAGCTATAttttttact                                                                                                  >  2:158045/4‑138 (MQ=1)
                                           gagtGTATTTAAGCTCTATATAAAAAGAACCGCAGGGCTCTTCAGATCTACGGATTTTCGACATGACACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttact                                                                                                  >  2:134238/4‑138 (MQ=1)
                                                tagttACGCTCTATATAAAAAGAACCGTAGGTCCTTTCAGCGCTACGGATTTTGCCCATGCCACGAAAGTAGCATCAGTCTAGCAAGTTAAACGAACACTGACATGATATATTATTGATTAGCTATATTTTTTACTTTGCa                                                                                            <  2:140875/138‑1 (MQ=1)
                                                tactgactctctATATAAAAAGAACCGCAGATCTCTTCAGCTCTAAGGATTTTCGCCAGGGCCGGAAATTAGCATCATGGTTGCCAGTTATGCGAACACTGACCTGATACATTAGTGGTTAGCTATATGTATTTACttttc                                                                                             >  2:68896/9‑139 (MQ=0)
                                                                  aaaGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTACTTTGCAACAGAACCTATAAAAAAc                                                                          >  2:40229/1‑141 (MQ=37)
                                                                      aaCCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCGTGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTGTTTACTTTGCAACAGAACCTATAAAAAACATg                                                                       >  1:264309/1‑141 (MQ=255)
                                                                          gCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTAAAGCGAAAACTGACATTATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCt                                                                   >  2:43107/1‑141 (MQ=255)
                                                                           cAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGACAACTCACCTAATAAATAAGTGGTTAGCTATATTTTTTTCATTTGCACCAGAACCTCTAAAAAAAATGGGCtt                                                                  >  1:80750/1‑141 (MQ=14)
                                                                            aGGTCTCTTCAGATCTACGGATTTTCGCAATACCCCGAAATTCGAATGGAGCTAGCAAGTTAAGCGAACACTGACATGATAGATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTa                                                                 >  1:333172/1‑141 (MQ=14)
                                                                             ggTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaa                                                                >  1:176127/1‑141 (MQ=255)
                                                                              gTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGATATATTTTTTTACTTTGCAACAGAACCTATAAACAACAGAGGCTTaaa                                                               >  2:166001/1‑141 (MQ=255)
                                                                                ctctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaaaag                                                             <  1:254349/141‑3 (MQ=255)
                                                                                                          tGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaaa                                   >  1:193461/1‑141 (MQ=255)
                                                                                                          tGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaaa                                   >  1:193445/1‑141 (MQ=255)
                                                                                                            ccACGAAATTAGCATCATGCTAGCAAGTTAAGACAACACTGACCTGGTTAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                 <  1:268310/141‑1 (MQ=21)
                                                                                                             cACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAAGTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAt                                >  1:33858/1‑141 (MQ=255)
                                                                                                               cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTCATTTATATAGTATTACTGCAAACATTg                              >  1:117944/1‑141 (MQ=255)
                                                                                                                      aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTggg                       >  1:278679/1‑141 (MQ=255)
                                                                                                                            acgCTAGCCAATCAAGCGAACACTGACATGATAAATTAGTGGTTAGCTTTATGTGTTTACTTTGCAACAGAATCTATAAACAACATGGGTTTTAATGGTTTATTTAGTTAGTATTACCTCAAACATTGATTTGGGTCaaaa                 <  2:88509/139‑1 (MQ=18)
                                                                                                                             tGCTAGCAAGTTAAGCGAACACTGAAATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCaaaaa                <  2:48018/141‑1 (MQ=255)
                                                                                                                              gCTAGCAAGTTAAGCGAACACTGACATGATCAATTAGTGATTAGCTACAGTGTTTTACGCGGCGACGCAGCCTATAGAGAACATTTTCGTAAAAGGTTAAGTGATATATTATTACTGCAAACATTGATTTGGGTCAAAAAt               <  2:390794/141‑1 (MQ=12)
                                                                                                                                 aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATGGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg            >  1:368884/1‑141 (MQ=255)
                                                                                                                                 aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg            >  1:49416/1‑141 (MQ=255)
                                                                                                                                         aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTAGTGCAAACATTCATTTCGGTCAAAAATTAGGGGTATTa    >  2:185399/1‑141 (MQ=255)
                                                                                                                                         aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa    >  2:302435/1‑141 (MQ=255)
                                                                                                                                         aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa    >  2:147598/1‑141 (MQ=255)
                                                                                                                                           gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa  >  1:209897/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GTATCAAAATATCAATACAGCAAAGAATACTTTAAAAGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAA  >  CP000730/76750‑77029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: