Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,969 G→A 100% intergenic (+1027/‑107) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9690GA100.0% 96.4 / ‑6.5 34intergenic (+1027/‑107)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (20/13);  minor base T (0/1);  total (20/14)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAA  >  CP000730/76856‑77089
                                                                                                                  |                                                                                                                        
tGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaaa                                                                                               >  1:193445/1‑141 (MQ=255)
tGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaaa                                                                                               >  1:193461/1‑141 (MQ=255)
  ccACGAAATTAGCATCATGCTAGCAAGTTAAGACAACACTGACCTGGTTAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                                                                             <  1:268310/141‑1 (MQ=21)
   cACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAAGTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAt                                                                                            >  1:33858/1‑141 (MQ=255)
     cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTCATTTATATAGTATTACTGCAAACATTg                                                                                          >  1:117944/1‑141 (MQ=255)
            aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTggg                                                                                   >  1:278679/1‑141 (MQ=255)
                  acgCTAGCCAATCAAGCGAACACTGACATGATAAATTAGTGGTTAGCTTTATGTGTTTACTTTGCAACAGAATCTATAAACAACATGGGTTTTAATGGTTTATTTAGTTAGTATTACCTCAAACATTGATTTGGGTCaaaa                                                                             <  2:88509/139‑1 (MQ=18)
                   tGCTAGCAAGTTAAGCGAACACTGAAATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCaaaaa                                                                            <  2:48018/141‑1 (MQ=255)
                    gCTAGCAAGTTAAGCGAACACTGACATGATCAATTAGTGATTAGCTACAGTGTTTTACGCGGCGACGCAGCCTATAGAGAACATTTTCGTAAAAGGTTAAGTGATATATTATTACTGCAAACATTGATTTGGGTCAAAAAt                                                                           <  2:390794/141‑1 (MQ=12)
                       aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATGGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                                                        >  1:368884/1‑141 (MQ=255)
                       aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                                                        >  1:49416/1‑141 (MQ=255)
                               aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTAGTGCAAACATTCATTTCGGTCAAAAATTAGGGGTATTa                                                                >  2:185399/1‑141 (MQ=255)
                               aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                >  2:147598/1‑141 (MQ=255)
                               aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                                                                >  2:302435/1‑141 (MQ=255)
                                 gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa                                                              >  1:209897/1‑141 (MQ=255)
                                    aaCACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaaaa                                                           <  2:190859/141‑1 (MQ=255)
                                                  aaaTTAGTGGTTAGCTATATCTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCAt                                              <  1:347734/141‑1 (MQ=255)
                                                  aaaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAGAAATTAGGGGTATTAAAAAAATGAATGAATCATa                                             >  1:368486/1‑141 (MQ=255)
                                                    aTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAt                                           >  1:209270/1‑141 (MQ=255)
                                                          ggTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATc                                     <  2:193445/141‑1 (MQ=255)
                                                          aaataaatataATTTTTTACTTTGCAACAGAACCTAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAAAAAAAAAAAAAAAATGAATGAATCATAATTTTATc                                     <  2:193461/134‑1 (MQ=255)
                                                                 tataTTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGATTAATTTATATAGTATTACTGCAAACATTGATTGTTGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGa                              <  2:272964/141‑1 (MQ=255)
                                                                           ttACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAgg                     <  2:22326/141‑1 (MQ=255)
                                                                                 taaaccAGAAGCTAGAAAGAACATGGGCTTAAAAGGTTGTTTTATATAGTATTACTGCAAACATTGATATGGGCCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTGAGCAAGCGAATTGACGGGGTTaaaa               >  2:203225/6‑141 (MQ=255)
                                                                                 tGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTaaaa               >  2:297944/1‑141 (MQ=255)
                                                                                 tGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAAATAGGGGTATTAAAAAAATGAATGAATCCTAATTTTCTCAAGCTGGTCGgacgggctcaga               >  2:333593/1‑131 (MQ=255)
                                                                                    aaaagaaacaaaaaaaaaCAAGGGCATAAAAGGTTAATTTATATAGTATAACTGAAAACATTGACTTGATTCAAAAAGAAAAAAAAAAAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGc            <  2:38339/129‑1 (MQ=255)
                                                                                    aaCAGAACCTATAAAAAACATGGGCTTACAAGGTTAATCTATATAGTATTACTGCCAACATTGATCTGGGACCAAAATTAGGGGGCTTAAAAAAATGAATGAATCATAATTTTATAAAGCTGATTGGAGAGGCAaaaaaca            >  2:64308/1‑138 (MQ=255)
                                                                                        gAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATta        >  1:228578/1‑141 (MQ=255)
                                                                                          aCCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtata      >  1:319451/1‑141 (MQ=255)
                                                                                           ccTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatat     >  2:103650/1‑141 (MQ=255)
                                                                                           ccTAAAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatat     <  2:333172/141‑1 (MQ=255)
                                                                                            gtataAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTTCTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATtatata    <  2:273677/140‑1 (MQ=255)
                                                                                              aaaaaaaaaaTAGGCATAAAAGGATAATATAAATAATAATAATACAAACATTGATTTGGTTCAAAAATTAAAAAAAAAAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATaaa  <  2:259151/139‑1 (MQ=255)
                                                                                                                  |                                                                                                                        
TGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATCAAGCTGATTGGAGAGGTTAAAATGCATTATATAAA  >  CP000730/76856‑77089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: