Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 58.4% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT58.4% 12.6 / 108.5 106intergenic (+107/+1)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base T (24/35);  total (48/58)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

Only 100 of 110 total aligned reads displayed.

ATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35740‑36016
                                                                                                                                        |                                                                                                                                              
aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtcaa                                                                                                                                            <  1:84301/141‑4 (MQ=255)
aTTCAGTATAAAGTTATTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAAAAGGTTCTTTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                                                                                            <  2:30590/141‑1 (MQ=255)
   cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt‑‑t                                                                                                                                       >  1:79028/1‑141 (MQ=255)
    aGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑Tg                                                                                                                                      >  1:361765/1‑141 (MQ=255)
      tataAAGTTATTGGCGAAAAATGCTTACTTGCACAAAAGCTACAATTAAATTGTAAAGCAAAGTGAGTTGAAGGTTCTGTTGCAGATTTTGAAAATGTGGCGATGCTCTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCt                                                                                                                                    <  2:390835/141‑1 (MQ=12)
       ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAACTAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAATAATTTATCATGTCAGTGTTCGCTTAAAT‑‑CGCTa                                                                                                                                   >  2:152762/1‑141 (MQ=255)
       ataAAGTTTTTGGCGAAAAATGATTACTTGAAAAAGAGCTAAAATAAAATTGAGATCTAAAAAAAATAGAAGGTTATGTTGCAAAGTAAAAAAATATAGATAACCACTAATTTATCATGTAAGTGATAGATACACT‑‑TGCTa                                                                                                                                   >  2:152820/1‑141 (MQ=25)
       agaaaGTTTTTGGCGAAAAATGAGTACGTGAAAAAGAGCTAAAGTAAAATTGTGATCAAATAAAAATAGAAGGTACTGTTGCAAAGAAAAAAAATATAGCTGACCACTAATATAACATGTCAGTGTTCGCATAACtgactc                                                                                                                                     >  2:152768/3‑136 (MQ=255)
               tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCatgatg                                                                                                                           >  1:51269/1‑141 (MQ=255)
               tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCatgatg                                                                                                                           >  2:33293/1‑141 (MQ=255)
                  ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTa                                                                                                                        <  2:103130/141‑1 (MQ=255)
                     gAAAAATGATTACTTTAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAAtt                                                                                                                     >  1:294749/1‑141 (MQ=255)
                      aaaaaaGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCGAATAACAATAGACGGTTCTGTTGCCAAGTACAAAAATATAGCTAACCACTGATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAAttt                                                                                                                    <  1:15612/141‑1 (MQ=21)
                               tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCATAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATg                                                                                                           >  2:220994/1‑141 (MQ=255)
                                aCTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATAATGTCAGAGTACGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATgg                                                                                                          >  2:88980/1‑141 (MQ=255)
                                   ttaaCAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGa                                                                                                       <  2:50736/139‑1 (MQ=255)
                                   tGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCTTGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGTTGCTATTTTCGTGGCATGGCGa                                                                                                       <  1:197718/141‑1 (MQ=255)
                                                  acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                      >  2:363379/15‑141 (MQ=11)
                                                  acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                      >  2:387443/15‑141 (MQ=11)
                                                   taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAGAATCCGTAGATCTGa                                                                                       >  2:74608/1‑141 (MQ=255)
                                                   caaaggagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     >  1:268552/14‑141 (MQ=11)
                                                    aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                    <  2:369002/129‑1 (MQ=11)
                                                     aaagagtattgtaataaAAATGATGATTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGCTCGATTAGCT‑‑TACTATCATGATGCTAATTTCGTGGCATGACAAAAGTCCGTAGATCTGAagag                                                                                   >  2:337085/12‑141 (MQ=2)
                                                      aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                                                  >  1:311166/11‑141 (MQ=11)
                                                      aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                                                  <  2:188235/131‑1 (MQ=11)
                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCG‑‑TAACTGCCCCGAGCATGATGCTTGAATCTTGGCATGGCGAAAATCCGTAGATTTGAAGAGAc                                                                                 <  1:289441/132‑1 (MQ=2)
                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGGCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:178887/132‑1 (MQ=11)
                                                        gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  2:29740/133‑1 (MQ=11)
                                                          gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  2:270921/135‑1 (MQ=11)
                                                           tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAACAGTAGATCTGCagaaaact                                                                               >  2:349245/1‑136 (MQ=255)
                                                           tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             >  2:274299/6‑141 (MQ=11)
                                                            attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTCGCCTGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  1:391118/137‑1 (MQ=11)
                                                            attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  1:157762/137‑1 (MQ=11)
                                                            attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  1:357293/137‑1 (MQ=11)
                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCgg                                                                           >  2:385487/4‑141 (MQ=11)
                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  2:365927/138‑1 (MQ=11)
                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  2:59277/138‑1 (MQ=11)
                                                              tttaataaAAATGATGGTTCTGTTGCAAAGTCAAAAATATAGCTAATCACTAATATATCATGTGAGTGTTCGTTTAACT‑‑TAGTAGCATGATGCTAATTTCGTGGGATGGGAAAAATCGGTAGGTATGAGAAGACCAGCggg                                                                          >  2:3582/3‑140 (MQ=2)
                                                              tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:88204/139‑1 (MQ=11)
                                                              tttaataaAAATGATGGTTCTGGTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:235082/139‑1 (MQ=11)
                                                              tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  2:131790/141‑1 (MQ=255)
                                                                taataaAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTGACCCCTAATTTATCATGTCCGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  1:215785/141‑1 (MQ=255)
                                                                 aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTACTATATCATGTCAGTGTTCATAAAACT‑‑TAATAGCAAGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCACTGGAAAGACCTGCCGTCCt                                                                       >  2:238377/1‑141 (MQ=2)
                                                                 aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                       >  2:246254/1‑141 (MQ=11)
                                                                 aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                       <  1:213740/141‑1 (MQ=11)
                                                                  ataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                        >  1:134379/1‑141 (MQ=38)
                                                                  ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                      >  2:166983/1‑141 (MQ=11)
                                                                  ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                      >  1:215423/1‑141 (MQ=11)
                                                                   taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  1:344232/141‑1 (MQ=11)
                                                                   taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     >  1:394659/1‑141 (MQ=11)
                                                                   taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  2:282856/141‑1 (MQ=11)
                                                                   taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  1:311184/141‑1 (MQ=11)
                                                                   taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATACTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACTTGCGGTTCttt                                                                     >  1:123703/1‑141 (MQ=11)
                                                                     aaaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAACTGAAGAGACCTGCGGTTCttt                                                                     <  2:288962/141‑1 (MQ=25)
                                                                     aaaaGAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  1:313010/141‑1 (MQ=18)
                                                                            aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                              <  2:185097/141‑1 (MQ=11)
                                                                                ttatGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGACATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCg                                                         <  1:184088/138‑1 (MQ=1)
                                                                                 tccgTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCg                                                         <  2:346012/138‑1 (MQ=1)
                                                                                                aacaaTATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGGTGCTAATTTCGTGGCATGGCGAAAACCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATa                                          <  2:8910/138‑1 (MQ=0)
                                                                                                         gccaatcACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTaaa                                 <  1:36263/135‑1 (MQ=1)
                                                                                                            aatcACTAATATATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                              <  1:95547/138‑1 (MQ=0)
                                                                                                                cctaagatATCATGTCAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTTGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCt                          <  2:39706/134‑1 (MQ=1)
                                                                                                                               tattggtCGTTTAGCT‑‑GGCTAGAATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           <  2:38919/135‑1 (MQ=11)
                                                                                                                               taGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           <  2:123546/140‑1 (MQ=11)
                                                                                                                               cAGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           <  2:310032/141‑1 (MQ=2)
                                                                                                                                aGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt          >  1:162052/1‑141 (MQ=11)
                                                                                                                                aGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:396417/1‑141 (MQ=9)
                                                                                                                                aGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  2:266248/1‑141 (MQ=14)
                                                                                                                                aGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  1:22844/141‑1 (MQ=14)
                                                                                                                                aGTGTTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:351080/1‑141 (MQ=9)
                                                                                                                                aGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:179936/1‑141 (MQ=31)
                                                                                                                                aGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          >  1:353215/1‑141 (MQ=31)
                                                                                                                                 gttgtCGTTTAACT‑‑TGTTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         <  2:91139/137‑1 (MQ=11)
                                                                                                                                 gtgtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:810/1‑141 (MQ=12)
                                                                                                                                  tgtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  1:281085/1‑141 (MQ=11)
                                                                                                                                  tgtTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        <  1:338431/141‑1 (MQ=17)
                                                                                                                                  tgtTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt        >  1:362747/1‑141 (MQ=17)
                                                                                                                                   gtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGGGCGTAAATACATTCAATTCCTTTTCAAGTATTCTTTGCCGTATTGCTACTTTg       <  1:250619/141‑1 (MQ=11)
                                                                                                                                   gtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       <  1:179633/141‑1 (MQ=255)
                                                                                                                                   gtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       >  1:127596/1‑141 (MQ=255)
                                                                                                                                   gtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       <  1:26259/141‑1 (MQ=11)
                                                                                                                                   gtTCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg       <  1:199588/141‑1 (MQ=12)
                                                                                                                                    ttCGGTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:33608/141‑1 (MQ=17)
                                                                                                                                    ttCGGTTAACT‑‑TGCTAGCATGATGCTAATTTCATGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCATATTGATACTTTGa      >  1:283275/1‑141 (MQ=11)
                                                                                                                                    ttCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:345351/141‑1 (MQ=32)
                                                                                                                                     tCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTACATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt     <  2:259762/141‑1 (MQ=11)
                                                                                                                                     tCGTTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCATCGATCTTTAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  1:287075/141‑1 (MQ=11)
                                                                                                                                     tCGTTTAACT‑‑TGCTAGCATGATGCTAATTCCGGGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  1:112899/141‑1 (MQ=255)
                                                                                                                                     tCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATTCGTTGCTGAAATACACGCAATTCCTTGTAAAGTATTCTTTGCTGTATTTATACTTTTAt     <  2:180684/141‑1 (MQ=11)
                                                                                                                                     tCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  2:382958/141‑1 (MQ=17)
                                                                                                                                     tCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     >  1:103942/1‑141 (MQ=17)
                                                                                                                                     tCGCTTAACT‑‑TGCTAGCATGATGCTAATATCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATAACTTTTAAAGTATTCTTTGCTGTATTGATACTTTAAt     >  1:276014/1‑141 (MQ=255)
                                                                                                                                     tCGCGTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     >  1:269343/1‑141 (MQ=17)
                                                                                                                                      cGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:123922/1‑141 (MQ=17)
                                                                                                                                       gCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGGAGAGACCTGCGGTTCTTTTTATATAGAGCGTAGATACACTCAATACCTGTTAAAGTATTCTTTGCTGTCTTGTTACTTTgtttc   >  1:383632/1‑137 (MQ=11)
                                                                                                                                       gCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  2:396286/141‑1 (MQ=17)
                                                                                                                                       gCTTAACT‑‑TGCTAGCATGATGCTAATTGCGTGGCGTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTGTATATCGAGCGAAAATTCACTCAATATCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:220252/141‑1 (MQ=11)
                                                                                                                                       ccTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  1:153723/140‑1 (MQ=17)
                                                                                                                                        cTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  2:294749/141‑1 (MQ=17)
                                                                                                                                        cTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  1:373078/141‑1 (MQ=17)
                                                                                                                                        |                                                                                                                                              
ATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACT‑‑TGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35740‑36016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: