Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,125,066	G→C	100%	E729Q (GAG→CAG)	pycA →	pyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,125,066	0	G	C	100.0%	102.2 / NA	30	E729Q (GAG→CAG)	pycA	pyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/14);  total (16/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTT  >  CP000730/1124936‑1125201                                                                                                                                   |                                                                                                                                        aTCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATc                                                                                                                               <  1:63643/141‑1 (MQ=255) aTCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATAAGGCAGGCTTATTAAAACCTAAAGCCGCTTACGACATGATTGGTCAGTTAAAATc                                                                                                                               <  1:256697/141‑1 (MQ=255)  tCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCa                                                                                                                              >  2:382944/1‑141 (MQ=255)  tCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCa                                                                                                                              >  2:348981/1‑141 (MQ=255)    aaaCATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAACCGCTTACGAATTGATTGGTCAGTTAAAAACAGc                                                                                                                            >  2:237448/1‑141 (MQ=255)            ataCTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGAtt                                                                                                                    <  1:111313/141‑1 (MQ=255)               cTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGTACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTAc                                                                                                                 >  2:65611/1‑141 (MQ=255)                    gggTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATCGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAAtt                                                                                                            <  1:380331/139‑1 (MQ=255)                                        tAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCTTGGTAc                                                                                        <  2:46670/141‑1 (MQ=255)                                        tAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATAc                                                                                        <  1:357351/141‑1 (MQ=255)                                           agggttataatctGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAg                                                                                     <  1:341383/129‑1 (MQ=255)                                            gagTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGt                                                                                    >  1:143540/1‑141 (MQ=255)                                                  gAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAAt                                                                              >  1:151445/1‑141 (MQ=255)                                                  gAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAAt                                                                              >  1:200003/1‑141 (MQ=255)                                                   aaCGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATg                                                                             >  1:279497/1‑141 (MQ=255)                                                           ggTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGGAATGGTttatta                                                                     >  1:328090/1‑141 (MQ=255)                                                            gataccATATTTTAGCGATTAAAGATATGGCACGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAACCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTaa                                                                    <  1:72305/137‑1 (MQ=255)                                                                   tatTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATacaa                                                             <  1:391565/141‑1 (MQ=255)                                                                   aatTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATacaa                                                             <  1:391501/140‑1 (MQ=255)                                                                     ttttAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTAATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACAGTCATGATACAAGTGGGAATGGTTTATTAACATacaaaa                                                           >  1:116536/1‑140 (MQ=255)                                                                       ttAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCATCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATacaaacaa                                                         <  1:269930/141‑1 (MQ=255)                                                                                 aagtacgggCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGCTTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATg                                               <  1:27917/134‑1 (MQ=255)                                                                                 aaGATATGGCAGGCTTGTTAATACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATCGAtt                                               >  1:203373/1‑140 (MQ=255)                                                                                 aaGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATg                                               >  1:237399/1‑141 (MQ=255)                                                                                               ttattaAAACCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATc                                 >  1:74196/1‑141 (MQ=255)                                                                                                      aaCCTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATa                          >  1:326328/1‑141 (MQ=255)                                                                                                        ccTAAAGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAAAACGAAGAACATGCTGGTGTCGATATCATTGATAct                        <  1:191386/141‑1 (MQ=255)                                                                                                             aGCCGCTTACGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGt                   <  2:388938/141‑1 (MQ=255)                                                                                                                     aCGAATTGATTGGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGGGTCGATATCATTGATACTGCTGTTGCTTCaa           >  1:312496/1‑141 (MQ=255)                                                                                                                              ttgGTCAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGTGTGGtt  >  2:27625/1‑141 (MQ=255)                                                                                                                                   |                                                                                                                                        ATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAGTTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCTAAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGATTTACCAATTCATCTTCACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGTGTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTT  >  CP000730/1124936‑1125201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑