Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,851,260	C→A	100%	A115S (GCG→TCG)	acsA1 ←	acetate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,851,260	0	C	A	100.0%	73.6 / NA	28	A115S (GCG→TCG)	acsA1	acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/12);  total (16/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTC  >  CP000730/1851136‑1851394                                                                                                                             |                                                                                                                                       aaTGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGgt                                                                                                                        <  2:204393/141‑1 (MQ=255) aaTGCCTTAGTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCGTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGgt                                                                                                                        <  2:378109/141‑1 (MQ=255)  aTGCCTGATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCTCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGgtt                                                                                                                       >  2:291302/1‑140 (MQ=255)     ccTAATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCATTTTCCATAAATGCTTCAAATAACGGCCCTACAATTGCACCAATGATTAAATGACCTAACAACGAAAAATAGAGTTCAGGTGTAc                                                                                                                    <  2:45984/141‑1 (MQ=255)      cTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTAcg                                                                                                                   <  1:394705/141‑1 (MQ=255)      cTTATTAGTAACTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTAcg                                                                                                                   <  2:225072/141‑1 (MQ=255)                aTTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTATCAACGAAAAATATAGCCCAGGTGCACGCAACataaaa                                                                                                         >  2:31139/1‑140 (MQ=255)                            gCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTg                                                                                             >  1:153242/1‑141 (MQ=255)                             cTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAAAGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACCTAAATATAAATACACTGt                                                                                            >  2:161826/1‑141 (MQ=255)                              ttCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTc                                                                                           <  2:346467/141‑1 (MQ=255)                                     ttCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATTCTCTGTCACCTTTg                                                                                    <  2:126344/141‑1 (MQ=255)                                       ctctAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTTTc                                                                                  >  2:177353/1‑141 (MQ=255)                                        tctAATCTATCCGCAACTGCCATTTCCATAAATGCTTCAAATAACGGCCCAACAACTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTAAGGTGTACGCCACAAAAATATAAATACTCTGCCACCttttcca                                                                                 >  2:123329/1‑137 (MQ=255)                                            aTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCCCCTTTGTCAACtt                                                                             >  1:23119/1‑141 (MQ=255)                                            aTCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACtt                                                                             >  1:26603/1‑141 (MQ=255)                                                 tCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGCCATAAATATAAATACTCTGTCACCTTTGTCAACTTCTTCa                                                                        >  2:294820/1‑141 (MQ=255)                                                          gCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGAc                                                               >  2:202298/1‑141 (MQ=255)                                                                 ccATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACAt                                                        >  2:251230/1‑141 (MQ=255)                                                                             cAAATAACGGCCCAACAATTGCACGAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATTCTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTttat                                            <  2:251232/141‑1 (MQ=255)                                                                                 tAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCTACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGa                                        <  1:364013/141‑1 (MQ=255)                                                                                 aaaCGGCCCAACAATTGCACCAATTGTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGa                                        <  2:73096/140‑1 (MQ=255)                                                                                      gCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAAcc                                   >  1:61272/1‑141 (MQ=255)                                                                                        ccAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATACCCGt                                 >  1:4498/1‑141 (MQ=255)                                                                                                 gCACCAATTTCTTAAACACCTAACAACAAAAAACATCGTTCACGTGTACGCGACATAAATATAAATACTCTGTCCATTTTGGCATCTTGTGCATTTTCAGACAAAACATTCGCTTCTTTTTTAGATAACCGTTGCATATCt                        <  2:258044/141‑1 (MQ=255)                                                                                                              aaaCACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAAGATAAATACACTGTCACCTTGGTCAACTTCTGCATGTTCAGACAAAACATTCGCTACATTATTAGATAACGGTTGCATATCTGTATAAGTATACg           >  2:296528/1‑141 (MQ=255)                                                                                                                acacCTAACAACGAAAAATATAGTTCAGGTGTAAGCGAAGTAAATATAAATACACTGTCACATTTGTTTAATACTAAATGTAAAGACACAACAGGCGCCGCGCTATTAGATAACCCTTTCATATCTTTATAAGTATACGAt         >  2:190160/1‑141 (MQ=255)                                                                                                                    cTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCtt     >  1:162387/1‑141 (MQ=255)                                                                                                                       acaacGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTc  <  1:16272/141‑1 (MQ=255)                                                                                                                             |                                                                                                                                       AATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTC  >  CP000730/1851136‑1851394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑