Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,812 G→A 54.7% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8120GA54.7% 5.1 / 103.4 86intergenic (‑895/‑273)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/16);  new base A (22/25);  total (45/41)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01

Only 100 of 102 total aligned reads displayed.

TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC  >  CP000730/1929672‑1929950
                                                                                                                                            |                                                                                                                                                 
tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATAGTTGCCGATGATATAAtag                                                                                                                                                   <  1:173325/141‑1 (MQ=255)
 cgtcTACTTTCTTAAATTAACTATATCTAGTCTCATAGATTTTGTCCAATTAAGTGTAGACGATTCAATCTCTCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTTTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                                  <  1:334571/138‑1 (MQ=37)
 cGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGTATCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagt                                                                                                                                                  <  1:271542/141‑1 (MQ=255)
 cGCCTACTTTC‑TAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                                                 <  1:22727/141‑1 (MQ=255)
  ggcgtctgtcctAAATTAACAGTATCTATTCTCATAGNATTTGTCCAGTTTAGTGTAGACGTTTCGAGCTTTTGGCAGGAATCATATACCTTATCTGAAGCAAGTGACTGTGCTTGGATATGTGCCGATGATATAAtagta                                                                                                                                                 <  2:34004/130‑1 (MQ=255)
       cTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTc                                                                                                                                            >  1:246019/1‑141 (MQ=255)
                     cAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTTCCGATGATATAATAGTAGAGTCGCCTATCTCTCAgg                                                                                                                              >  1:121359/1‑141 (MQ=255)
                      aaTATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTCTCTCGCAGGc                                                                                                                             >  2:55347/1‑141 (MQ=255)
                         atCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATCGTAGAGTCGCCTATCTCTCAGGCGTc                                                                                                                          <  2:246019/141‑1 (MQ=255)
                              ttCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAttt                                                                                                                     <  2:321649/141‑1 (MQ=255)
                                   aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACg                                                                                                                <  2:192213/141‑1 (MQ=255)
                                      gAATTTGTCCAATTAAGTGTAGACGATTCAATCCATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                             <  1:24854/141‑1 (MQ=255)
                                       aaTTTGTCTAAGTAAGTGTAGACTACTCAATCTATCAGGTAGAATCATATAACTTAACAGAAGCAAGTGACTGTGCTTTTATCTTTGCCGATGATATAATAGCAGAGTCGCCTATCTCTCAGGCGTCAACTTAGACGCaga                                                                                                            <  2:257250/141‑1 (MQ=255)
                                             tCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgaggag                                                                                                      >  2:204145/1‑141 (MQ=255)
                                                        gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑t                                                                                      <  1:393039/141‑1 (MQ=255)
                                                          aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCG‑TA‑TAA‑A‑G‑T‑Ga                                                                                   >  1:101414/1‑141 (MQ=255)
                                                             cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTAGATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGc                                                                               <  1:275194/141‑1 (MQ=255)
                                                                ttCAATCTATAAGCGAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                            >  2:268194/1‑141 (MQ=255)
                                                                ttCAATCTATAAGCAAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                            >  2:268215/1‑141 (MQ=255)
                                                                    aTCTATCAGCTAGAATCATAGAACTTATCAGAAGCAAGTGACTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCGCTCAGGCGTCAATTTAGACTCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTAtttt                                                                        >  2:216176/1‑141 (MQ=255)
                                                                      cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCCATCAGGCGTCAATTTAGACTCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCg                                                                      <  1:93088/141‑1 (MQ=255)
                                                                        ttCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGtt                                                                    <  2:72215/140‑1 (MQ=255)
                                                                            acTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTcaca                                                                <  1:28266/140‑1 (MQ=255)
                                                                                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAg                                                          >  2:326067/1‑141 (MQ=255)
                                                                                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAACAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTGTTTTCGTTGACATCATAg                                                          >  2:149856/1‑141 (MQ=255)
                                                                                   ggatctcagtccacAGATGCAAGTGACTGTGCTCGTATCTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGc                                                         >  2:86747/14‑141 (MQ=255)
                                                                                         aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGt                                                   >  2:396339/1‑141 (MQ=255)
                                                                                               tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GG‑TGCTTATTTTCGTTCACATCATAGAACCAGCcagcag                                             >  2:174291/1‑141 (MQ=255)
                                                                                                  gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGAACCAGTCATCAGTgg                                          >  1:61762/1‑141 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCTTAGCACCAGTCATCAGTGGc                                         <  1:239216/127‑1 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATCGCCCCAGTCATCAGGGGc                                         >  2:230999/15‑141 (MQ=255)
                                                                                                   aaGCAAGTGACTGTGCTTGTATATTTGCCGATCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGATG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                         <  1:264700/141‑1 (MQ=255)
                                                                                                    cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGAtg                                       >  2:293589/13‑141 (MQ=255)
                                                                                                     ggaagtcgatatgCTTGTATATTCACAGCTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTCCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                      <  1:164810/130‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGGTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtc                                     >  1:85308/11‑140 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                     >  1:167636/11‑141 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATAATCCCAGACGGTATCATAAAAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGACGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCGCCAGTCATCAGTGGCtgtg                                     <  1:209770/131‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATAATCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAACCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                     >  1:241671/11‑141 (MQ=255)
                                                                                                       gagtagatattgttGTACATTCACGGATGGTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                    <  2:351849/129‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                    <  1:361961/132‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                    <  1:211653/132‑1 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                   <  1:268087/133‑2 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                  >  1:175061/8‑139 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCCCATCCTAGCACCAGTCATCAGTGGCTGTGctg                                  >  1:158112/8‑139 (MQ=255)
                                                                                                         gtaaatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                  <  2:153668/134‑3 (MQ=255)
                                                                                                         gTGACTGTGCTTGTATATTTGGCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCCCATCATCGCACTAGTCATCAGTGGCTGTGcc                                   >  1:203355/1‑141 (MQ=255)
                                                                                                         gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                                   <  1:204145/141‑1 (MQ=255)
                                                                                                          tGACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGccc                                  >  1:269461/1‑140 (MQ=255)
                                                                                                           agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCAACAGTCATCAGTGGCTGTGctgtt                                >  2:18440/6‑137 (MQ=255)
                                                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                               <  2:175540/137‑6 (MQ=255)
                                                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                               >  1:367696/5‑136 (MQ=255)
                                                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                               <  2:372132/137‑6 (MQ=255)
                                                                                                              tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                              >  1:262361/1‑141 (MQ=255)
                                                                                                              tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                             >  2:346108/3‑141 (MQ=255)
                                                                                                                  cTTGTATAGTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                         <  2:21179/141‑4 (MQ=255)
                                                                                                                   ttGTATATTCACAGATGGTTTAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                        <  2:4917/141‑5 (MQ=255)
                                                                                                                    tGTATATTTGCCGATGATATAATAGGAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GC‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                        <  1:41819/141‑1 (MQ=255)
                                                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCTTCAGTGGCTGTGCTGTTGCGtattat                       <  1:375820/141‑6 (MQ=255)
                                                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAAGCATATATTT‑‑CTTTAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                       <  1:11342/141‑6 (MQ=255)
                                                                                                                     gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGACTGTGCTGTTGCGtattata                      >  2:25995/12‑138 (MQ=255)
                                                                                                                      tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                      <  1:396339/141‑1 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                     <  2:300593/141‑8 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                     <  2:170422/141‑8 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                     <  1:159110/141‑5 (MQ=255)
                                                                                                                        tattcactgatggtATAATAATAGATTCGCCTATCTCTGAGGCGTCAA‑‑TGGACGAAGAGAGGAGGTGCGT‑TAC‑T‑T‑T‑GC‑TAAGCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                   <  1:178478/133‑7 (MQ=255)
                                                                                                                         attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                  >  2:327679/8‑141 (MQ=255)
                                                                                                                         attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TACTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                  >  1:379275/8‑141 (MQ=255)
                                                                                                                           ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑CGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                 <  1:60499/141‑1 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGCGCTGTTGCGTATTATACGTATTg               >  1:210517/5‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATCCTTATTg               >  1:134399/5‑141 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg               >  1:331669/5‑141 (MQ=255)
                                                                                                                            cacagacggaaaaataatAGATTCGCCTATCATTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg               <  1:138513/129‑1 (MQ=255)
                                                                                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCGTATTATACTTATTgg              <  1:85048/138‑1 (MQ=255)
                                                                                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTGC‑T‑T‑T‑GC‑TAATCATTTTCGTTCAGATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTCTTATACTTATTggt             >  2:223025/3‑140 (MQ=255)
                                                                                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc             >  1:154708/3‑141 (MQ=255)
                                                                                                                                 atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta          >  1:317410/5‑139 (MQ=255)
                                                                                                                                  tggtataATCATAGGGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag         <  2:173884/138‑4 (MQ=255)
                                                                                                                                  tggtagaataatAGAGTCGCTTATCTCTCAGGCGTCAATT‑‑GACGGAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag         <  2:69275/135‑4 (MQ=255)
                                                                                                                                  tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTTTTGGCTaa          >  2:63992/1‑141 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcctagt        <  2:240761/139‑6 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt        <  1:198195/139‑1 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt        <  1:117363/139‑1 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCGCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt        <  2:276286/139‑1 (MQ=255)
                                                                                                                                   gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg         >  2:323567/1‑141 (MQ=255)
                                                                                                                                   gatATAATAGTAGAGTCGCCTATCTCTCAGGCGACAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATCTTGGCTAAg         >  2:211056/1‑141 (MQ=255)
                                                                                                                                    gtataATAATAGAGTCGCCTATCCCTCAGGCGCCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa       <  1:755/140‑1 (MQ=255)
                                                                                                                                    atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACAGCGTAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt        >  2:217750/1‑141 (MQ=255)
                                                                                                                                     tataATAGTAGAGTCGCCTATCTCTTAGGCGTAAATTTAGACGCAGAGAGGAGGGG‑TG‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGtt       >  2:273363/1‑140 (MQ=255)
                                                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTAGCTGTGCTGTTGCGTATGATACTTATTGGCTGAGTaa      >  2:236414/1‑140 (MQ=255)
                                                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGCGGAGGTG‑CA‑TTA‑G‑T‑TAGT‑TACTCATTTTGGCTTATATCATAGAACCAGTTCTCAGTGGCTGTGTGTTTGCTGTTTATACTTATTGGCTTAGTaa      >  2:329658/1‑140 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACTAAGAGAGGAGGTG‑CA‑TTTCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa     <  1:178075/141‑3 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGGATTATACTTATTGGCTTAGTaaa     >  2:86108/1‑139 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa     >  1:27226/1‑139 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa     <  1:99730/141‑3 (MQ=255)
                                                                                                                                         ataataGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc  <  1:107586/141‑1 (MQ=255)
                                                                                                                                         ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAGTGGCTTAGTAAACGc  <  1:294771/141‑1 (MQ=255)
                                                                                                                                         ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc  <  1:120251/141‑1 (MQ=255)
                                                                                                                                         ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAATCACTAAGTTGAcc   >  1:148590/1‑140 (MQ=255)
                                                                                                                                          tagtagAGTCGCCGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc  <  1:147474/141‑1 (MQ=255)
                                                                                                                                          atgtagAGTCGTCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc  <  1:267220/139‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC  >  CP000730/1929672‑1929950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑