Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,848 C→A 63.5% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8480CA63.5% 108.7 / 127.2 138intergenic (‑931/‑237)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base A (41/45);  total (66/72)
Fisher's exact test for biased strand distribution p-value = 8.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAA  >  CP000730/1929710‑1929985
                                                                                                                                          |                                                                                                                                                
gAATTTGTCCAATTAAGTGTAGACGATTCAATCCATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                                                                <  1:24854/141‑1 (MQ=255)
 aaTTTGTCTAAGTAAGTGTAGACTACTCAATCTATCAGGTAGAATCATATAACTTAACAGAAGCAAGTGACTGTGCTTTTATCTTTGCCGATGATATAATAGCAGAGTCGCCTATCTCTCAGGCGTCAACTTAGACGCaga                                                                                                                                               <  2:257250/141‑1 (MQ=255)
                  gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑t                                                                                                                         <  1:393039/141‑1 (MQ=255)
                    aGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGCG‑TA‑TAA‑A‑G‑T‑Ga                                                                                                                      >  1:101414/1‑141 (MQ=255)
                       cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTAGATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGc                                                                                                                  <  1:275194/141‑1 (MQ=255)
                          ttCAATCTATAAGCGAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                                                               >  2:268194/1‑141 (MQ=255)
                          ttCAATCTATAAGCAAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                                                               >  2:268215/1‑141 (MQ=255)
                              aTCTATCAGCTAGAATCATAGAACTTATCAGAAGCAAGTGACTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCGCTCAGGCGTCAATTTAGACTCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTAtttt                                                                                                           >  2:216176/1‑141 (MQ=255)
                                cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCCATCAGGCGTCAATTTAGACTCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCg                                                                                                         <  1:93088/141‑1 (MQ=255)
                                      acTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTcaca                                                                                                   <  1:28266/140‑1 (MQ=255)
                                             ggatctcagtccacAGATGCAAGTGACTGTGCTCGTATCTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGc                                                                                            >  2:86747/14‑141 (MQ=255)
                                                         tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GG‑TGCTTATTTTCGTTCACATCATAGAACCAGCcagcag                                                                                >  2:174291/1‑141 (MQ=255)
                                                            gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGAACCAGTCATCAGTgg                                                                             >  1:61762/1‑141 (MQ=255)
                                                            atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCTTAGCACCAGTCATCAGTGGc                                                                            <  1:239216/127‑1 (MQ=255)
                                                            atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATCGCCCCAGTCATCAGGGGc                                                                            >  2:230999/15‑141 (MQ=255)
                                                             aaGCAAGTGACTGTGCTTGTATATTTGCCGATCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGATG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                            <  1:264700/141‑1 (MQ=255)
                                                              cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGAtg                                                                          >  2:293589/13‑141 (MQ=255)
                                                                gaagtagatatgCTTGTATATTCACAGGTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtc                                                                        >  1:85308/11‑140 (MQ=255)
                                                                gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                        >  1:167636/11‑141 (MQ=255)
                                                                gaagtagatatgCTTGTATAATCCCAGACGGTATCATAAAAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGACGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCGCCAGTCATCAGTGGCtgtg                                                                        <  1:209770/131‑1 (MQ=255)
                                                                gaagtagatatgCTTGTATAATCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAACCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                        >  1:241671/11‑141 (MQ=255)
                                                                 gagtagatattgttGTACATTCACGGATGGTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                       <  2:351849/129‑1 (MQ=255)
                                                                 aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                       <  1:361961/132‑1 (MQ=255)
                                                                 aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                       <  1:211653/132‑1 (MQ=255)
                                                                   gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                     >  1:175061/8‑139 (MQ=255)
                                                                   gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCCCATCCTAGCACCAGTCATCAGTGGCTGTGctg                                                                     >  1:158112/8‑139 (MQ=255)
                                                                   gtaaatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                     <  2:153668/134‑3 (MQ=255)
                                                                   gTGACTGTGCTTGTATATTTGGCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCCCATCATCGCACTAGTCATCAGTGGCTGTGcc                                                                      >  1:203355/1‑141 (MQ=255)
                                                                    tGACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGccc                                                                     >  1:269461/1‑140 (MQ=255)
                                                                     agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCAACAGTCATCAGTGGCTGTGctgtt                                                                   >  2:18440/6‑137 (MQ=255)
                                                                      gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                  >  1:367696/5‑136 (MQ=255)
                                                                      gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                  <  2:372132/137‑6 (MQ=255)
                                                                      gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                  <  2:175540/137‑6 (MQ=255)
                                                                        tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                 >  1:262361/1‑141 (MQ=255)
                                                                        tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                >  2:346108/3‑141 (MQ=255)
                                                                            cTTGTATAGTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                            <  2:21179/141‑4 (MQ=255)
                                                                             ttGTATATTCACAGATGGTTTAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                           <  2:4917/141‑5 (MQ=255)
                                                                              tGTATATTTGCCGATGATATAATAGGAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GC‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                           <  1:41819/141‑1 (MQ=255)
                                                                              tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAAGCATATATTT‑‑CTTTAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                          <  1:11342/141‑6 (MQ=255)
                                                                               gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGACTGTGCTGTTGCGtattata                                                         >  2:25995/12‑138 (MQ=255)
                                                                                tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                                                         <  1:396339/141‑1 (MQ=255)
                                                                                tataTTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                        <  1:159110/141‑5 (MQ=255)
                                                                                   attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                     >  2:327679/8‑141 (MQ=255)
                                                                                   attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TACTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                     >  1:379275/8‑141 (MQ=255)
                                                                                     ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑CGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                                                    <  1:60499/141‑1 (MQ=255)
                                                                                      cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGCGCTGTTGCGTATTATACGTATTg                                                  >  1:210517/5‑141 (MQ=255)
                                                                                      cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATCCTTATTg                                                  >  1:134399/5‑141 (MQ=255)
                                                                                      cacagatggtATAATAATAGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                  >  1:331669/5‑141 (MQ=255)
                                                                                      cacagacggaaaaataatAGATTCGCCTATCATTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                  <  1:138513/129‑1 (MQ=255)
                                                                                        cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTGC‑T‑T‑T‑GC‑TAATCATTTTCGTTCAGATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTCTTATACTTATTggt                                                >  2:223025/3‑140 (MQ=255)
                                                                                           atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                             >  1:317410/5‑139 (MQ=255)
                                                                                            tggtataATCATAGGGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                            <  2:173884/138‑4 (MQ=255)
                                                                                            tggtagaataatAGAGTCGCTTATCTCTCAGGCGTCAATT‑‑GACGGAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                            <  2:69275/135‑4 (MQ=255)
                                                                                            tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTTTTGGCTaa                                             >  2:63992/1‑141 (MQ=255)
                                                                                             ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                           <  1:198195/139‑1 (MQ=255)
                                                                                             gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                            >  2:323567/1‑141 (MQ=255)
                                                                                             gatATAATAGTAGAGTCGCCTATCTCTCAGGCGACAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATCTTGGCTAAg                                            >  2:211056/1‑141 (MQ=255)
                                                                                              gtataATAATAGAGTCGCCTATCCCTCAGGCGCCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                          <  1:755/140‑1 (MQ=255)
                                                                                               tataATAGTAGAGTCGCCTATCTCTTAGGCGTAAATTTAGACGCAGAGAGGAGGGG‑TG‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGtt                                          >  2:273363/1‑140 (MQ=255)
                                                                                               tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTAGCTGTGCTGTTGCGTATGATACTTATTGGCTGAGTaa                                         >  2:236414/1‑140 (MQ=255)
                                                                                               tataATAATAGAGTCGCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGCGGAGGTG‑CA‑TTA‑G‑T‑TAGT‑TACTCATTTTGGCTTATATCATAGAACCAGTTCTCAGTGGCTGTGTGTTTGCTGTTTATACTTATTGGCTTAGTaa                                         >  2:329658/1‑140 (MQ=255)
                                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACTAAGAGAGGAGGTG‑CA‑TTTCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                        <  1:178075/141‑3 (MQ=255)
                                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGGATTATACTTATTGGCTTAGTaaa                                        >  2:86108/1‑139 (MQ=255)
                                                                                                ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                        >  1:27226/1‑139 (MQ=255)
                                                                                                ataataataGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                        <  1:99730/141‑3 (MQ=255)
                                                                                                 taatgatagAGTCGCCTATCTCTCAGGCGGCA‑‑GTGGACGAGGAGAGGAGGGGCAA‑TAC‑T‑T‑G‑GG‑TAATCATTTTCGTGCACATCATAGCACCAGTCAGCAGTGGCCGTGCGTTTGCGTATTCTAACTATTGGCttggtaacc                                       >  2:355167/7‑133 (MQ=255)
                                                                                                   ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAA‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                     >  1:32389/5‑141 (MQ=255)
                                                                                                   ataataGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                     <  1:107586/141‑1 (MQ=255)
                                                                                                   ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAGTGGCTTAGTAAACGc                                     <  1:294771/141‑1 (MQ=255)
                                                                                                   ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                     <  1:120251/141‑1 (MQ=255)
                                                                                                   ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAATCACTAAGTTGAcc                                      >  1:148590/1‑140 (MQ=255)
                                                                                                   agaagagcggcGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCAGCAGTGGCTTTGCCGTTGCTTATTATACTTATTGGCTTAGTAAACGc                                     <  2:143877/131‑1 (MQ=255)
                                                                                                    atgtagAGTCGTCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                     <  1:267220/139‑1 (MQ=255)
                                                                                                    aaatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                    <  2:203161/138‑1 (MQ=255)
                                                                                                     aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                   >  1:69935/3‑141 (MQ=255)
                                                                                                     aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                   <  1:59507/139‑1 (MQ=255)
                                                                                                     aatagAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCCCCCATTAGTAGTGGCTGGTTTCTTTCGTATTATACTTATTGGCTTAGTAAACGCaa                                   <  1:92973/139‑1 (MQ=255)
                                                                                                        agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                >  1:231743/1‑138 (MQ=255)
                                                                                                        agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACAGCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTAGTATACTTCTTGGATTAGTAAACGCaacaa                                >  2:169649/1‑138 (MQ=255)
                                                                                                        agagTCCCCTATTTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                <  2:10224/141‑4 (MQ=255)
                                                                                                          agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                               <  1:370019/141‑1 (MQ=255)
                                                                                                          agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                              <  1:228455/141‑6 (MQ=255)
                                                                                                              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGccacaaataatt                          >  2:128686/1‑130 (MQ=255)
                                                                                                              gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                          >  1:353055/1‑132 (MQ=255)
                                                                                                               ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGGAGCAATACCAAATa                          >  2:350998/1‑141 (MQ=255)
                                                                                                               ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                          <  2:224689/141‑1 (MQ=255)
                                                                                                                cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                        <  2:367696/141‑12 (MQ=255)
                                                                                                                 tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                       <  2:315908/141‑13 (MQ=255)
                                                                                                                  aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                       <  1:326067/141‑1 (MQ=255)
                                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCCTATTAGCTTAGTAAACGaaacaaataattaata                      >  1:304464/1‑128 (MQ=255)
                                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                      >  1:149819/1‑128 (MQ=255)
                                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                      >  2:134852/1‑128 (MQ=255)
                                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaaa                      >  1:385276/1‑128 (MQ=255)
                                                                                                                  aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcatc                      >  2:293934/1‑128 (MQ=255)
                                                                                                                          ggCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata               >  1:159331/1‑141 (MQ=255)
                                                                                                                           gCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg              <  1:335541/141‑1 (MQ=255)
                                                                                                                              ccAATGTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCg           <  2:170324/140‑1 (MQ=255)
                                                                                                                                 aTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAc        >  1:307362/1‑141 (MQ=255)
                                                                                                                                       aCGCAGAGGTGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaa  <  2:220766/141‑1 (MQ=255)
                                                                                                                                       aCGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaa  <  2:287627/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                
GAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAA  >  CP000730/1929710‑1929985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑