BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,843	Δ1 bp	51.6%	intergenic (‑926/‑242)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,843	0	A	.	51.6%	inf / inf	215	intergenic (‑926/‑242)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (49/51); new base . (58/53); total (108/107)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

TAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAT  >  CP000730/1929708‑1929983

tAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGc                                                                                                                                             <  1:393963/141‑1 (MQ=255)

  gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCag                                                                                                                                           <  2:281128/141‑1 (MQ=255)

   aaTTTGTCCAATTATGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATCGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACTCaga                                                                                                                                          <  1:757956/141‑1 (MQ=255)

       tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCCATT‑TAGACGCAGAgagg                                                                                                                                      >  2:272553/1‑141 (MQ=255)

        gTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAgaggg                                                                                                                                     >  1:301165/1‑140 (MQ=255)

                    gtAGACGATTCAATCTATCAGCGAGAATCATATAACTTACAAGAAGCAAGTGACTGTGCTTGTAAATTTGCCGATGGTATAATAGTAGAGGCGGCTAACTATCAGGCGTCAATT‑TAGACGGAGAGAGGAGGTGtg‑taggat                                                                                                                        >  1:102090/1‑137 (MQ=255)

                     tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑Tg                                                                                                                    >  1:638417/1‑141 (MQ=255)

                     tAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑Tg                                                                                                                    >  1:113503/1‑141 (MQ=255)

                        aCGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATg                                                                                                                 <  2:1003221/141‑1 (MQ=255)

                           aTTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGAGTA‑TAA‑G‑G‑TGATGCtt                                                                                                              >  1:188102/1‑141 (MQ=255)

                             tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTG‑TAA‑G‑G‑TGATGCTTAt                                                                                                            >  2:774601/1‑141 (MQ=255)

                              cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTAtt                                                                                                           >  1:851058/1‑141 (MQ=255)

                                     tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTc                                                                                                    <  2:29043/141‑1 (MQ=255)

                                     tCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTc                                                                                                    <  2:442753/141‑1 (MQ=255)

                                        gCTAGAATCATATAACTTATCGGGAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTcaca                                                                                                 <  1:797998/141‑1 (MQ=255)

                                        gCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTcaca                                                                                                 <  1:207966/141‑1 (MQ=255)

                                         cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCAcat                                                                                                >  1:764043/1‑141 (MQ=255)

                                         cTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCAcat                                                                                                <  1:106887/141‑1 (MQ=255)

                                             aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATa                                                                                            >  2:1042748/1‑141 (MQ=255)

                                             aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATa                                                                                            <  2:447528/141‑1 (MQ=255)

                                                 atatAACTTATCAGAAGCAAGTGCCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCAc                                                                                        <  2:795196/141‑1 (MQ=255)

                                                     aaCTTATGAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                    <  2:405118/141‑1 (MQ=255)

                                                     aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCGGGGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                    <  1:201705/141‑1 (MQ=255)

                                                     aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                    <  2:236841/141‑1 (MQ=255)

                                                               aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                          <  1:348002/141‑1 (MQ=255)

                                                                 ttaactagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                       <  2:558935/130‑1 (MQ=255)

                                                                 ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                       <  1:985514/130‑1 (MQ=255)

                                                                 gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                        >  1:1023864/1‑141 (MQ=255)

                                                                  gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                      <  2:603360/131‑1 (MQ=255)

                                                                   aagtagatatgattGTATATTCACAGAGGATATAAGAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGAAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                     >  2:970568/13‑141 (MQ=255)

                                                                    agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                    <  1:806288/133‑2 (MQ=255)

                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                   <  1:69341/134‑3 (MQ=255)

                                                                     gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                   >  2:877495/8‑139 (MQ=255)

                                                                      gagatatgCTTGTATATTCACAGATGGTATAATCATAGAGTCGCGTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                  <  1:26320/135‑4 (MQ=255)

                                                                       gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                  >  2:399780/1‑141 (MQ=255)

                                                                       gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                  <  1:278438/141‑1 (MQ=255)

                                                                       agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                 >  1:767931/6‑137 (MQ=255)

                                                                       agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                 >  1:84295/6‑137 (MQ=255)

                                                                        gatacgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                >  1:720912/6‑136 (MQ=255)

                                                                         atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                               <  1:344168/138‑7 (MQ=255)

                                                                              cTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttt                                                           >  1:584821/1‑141 (MQ=255)

                                                                              cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtgtt                                                          >  1:322164/1‑141 (MQ=255)

                                                                               ttgttgatTTGCCGATGAGATAAGAGTAGAGTCTCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                          <  2:954764/135‑1 (MQ=255)

                                                                               ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCATGCGTCAATT‑TAGCCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                          <  2:866725/141‑1 (MQ=255)

                                                                               ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                          <  2:974418/141‑1 (MQ=255)

                                                                               ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                          <  2:16197/141‑1 (MQ=255)

                                                                               ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                          <  2:385319/141‑1 (MQ=255)

                                                                                tgtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                        >  2:527972/13‑139 (MQ=255)

                                                                                tGTATATTTGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTTAATT‑AAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                         <  1:933634/141‑1 (MQ=255)

                                                                                tGTATATTCGCCGATGATATAATATTAGAGTCGCCTATCCCTCAGGCGTAAATC‑TAGACACAGAGAGAAGATTAA‑AAA‑G‑G‑CGATGCTGAGTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGttttt                                                         <  2:640740/141‑1 (MQ=255)

                                                                                 gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                       <  2:782078/130‑4 (MQ=255)

                                                                                   atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATAAGTGGCTGTGCTGTTGCGtattatact                                                     >  1:214158/10‑136 (MQ=255)

                                                                                    tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                    >  2:172044/9‑135 (MQ=255)

                                                                                    tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                    >  1:894127/9‑141 (MQ=255)

                                                                                     attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTGATCAGTGGCTGTGCTGTTGCGTATTATACttt                                                   >  2:513247/8‑140 (MQ=255)

                                                                                        tGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGACGCCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTATTTCTCTTAtt                                                 >  2:175705/1‑141 (MQ=255)

                                                                                         acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATTGAAGAGGCATGGACG‑GGCAT‑‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                               <  1:823026/138‑1 (MQ=255)

                                                                                         acagatgggataataAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                               <  2:826198/132‑1 (MQ=255)

                                                                                           agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGCGGAGCTTCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                             <  1:1040905/140‑1 (MQ=255)

                                                                                            gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                            >  2:326619/1‑140 (MQ=255)

                                                                                            gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                             >  1:542007/1‑141 (MQ=255)

                                                                                             atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                           >  1:64641/5‑139 (MQ=255)

                                                                                              tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                          <  1:175163/138‑4 (MQ=255)

                                                                                              tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑TGCTAATCATTTTCGTTCACATTATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                          >  2:583068/4‑141 (MQ=255)

                                                                                               ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                         <  2:186077/139‑1 (MQ=255)

                                                                                               ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                         >  1:1049589/3‑141 (MQ=255)

                                                                                               ggtataAGAATCGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                         <  2:33520/139‑1 (MQ=255)

                                                                                               ggtataAAAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                         <  2:614834/139‑1 (MQ=255)

                                                                                                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACTTAGTa                                        >  1:59171/2‑141 (MQ=255)

                                                                                                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                        <  2:539936/140‑1 (MQ=255)

                                                                                                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGAAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                        <  1:200143/140‑1 (MQ=255)

                                                                                                  ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                      >  2:144909/1‑139 (MQ=255)

                                                                                                  ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCGTTGCGTATTATACTTATTGGCTTAGTaaa                                      <  2:911961/141‑3 (MQ=255)

                                                                                                   taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                     <  2:810339/141‑4 (MQ=255)

                                                                                                     ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                   <  2:944018/141‑1 (MQ=255)

                                                                                                         tagaggcctcTATCTCTCAGGTGTCAATT‑‑‑GACGAAGTGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTGCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTCTTGGCTTAGTAAACGCaaca                               <  1:746053/132‑3 (MQ=255)

                                                                                                         tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                               >  2:737965/1‑139 (MQ=255)

                                                                                                            agTCGCCTATCTCTCAGGCGGCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCGTAGCACCAGGCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAAcacat                            >  1:610664/1‑140 (MQ=255)

                                                                                                               cGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                          >  1:138422/1‑141 (MQ=255)

                                                                                                               cGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGGAAACGCaacaaataat                         >  2:344020/1‑133 (MQ=255)

                                                                                                                gCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                         >  1:598935/1‑141 (MQ=255)

                                                                                                                 ccTATCTCTCAGGCGTCAATT‑‑‑GACGAAGATAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                       <  2:42778/141‑11 (MQ=255)

                                                                                                                 ccTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                       <  2:985247/141‑11 (MQ=255)

                                                                                                                  cTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                      >  1:975657/1‑130 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                     >  2:846315/1‑141 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGACATTGCGTTTTTTTCTTATTGGTTAAGTAGACGCACTATAAAATAggg                     >  2:958987/1‑140 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                    >  2:695494/1‑128 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                    >  1:418314/1‑128 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAAGCaacaaataattaata                    >  1:712638/1‑128 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACTTAGTAAACGCaacaaataattaata                    >  2:89379/1‑128 (MQ=255)

                                                                                                                    aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                    <  2:256161/141‑14 (MQ=255)

                                                                                                                     tctctcAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTTTTGTCTATTATACTTATTGGCTTAGTAAACGCaacaaataattaagag                   <  2:437983/141‑15 (MQ=255)

                                                                                                                     tctctcAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                   <  2:1062047/141‑15 (MQ=255)

                                                                                                                      ttctgAGGCTTCAAGT‑TAGACGCAGAGAGGAGGGGTA‑GAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGa                   <  1:441641/140‑1 (MQ=255)

                                                                                                                      ctttcAGGCGTCAATT‑TAGACGCAGAGAGTAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGa                   <  1:286633/138‑1 (MQ=255)

                                                                                                                          cAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata               <  2:178970/141‑1 (MQ=255)

                                                                                                                          cAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata               <  1:399780/141‑1 (MQ=255)

                                                                                                                                tCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCg         >  1:904700/1‑141 (MQ=255)

                                                                                                                                  aaTT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa       >  2:925655/1‑141 (MQ=255)

                                                                                                                                    tt‑tAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCACTAGAAAATAGGTGACATATAGCCGCAcc     >  1:487394/1‑141 (MQ=255)

TAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAT  >  CP000730/1929708‑1929983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`