Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 58.7% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG58.7% 612.7 / inf 1068C155C (TGT→TGCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (226/213);  new base G (304/315);  total (537/531)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 1073 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226
                                                                                                                                            |                                                                                                                                           
tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                                                                                                                                             >  1:360333/1‑141 (MQ=40)
  tttATATAGAGCGGAAATACACGCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCa                                                                                                                                           <  1:930542/141‑1 (MQ=255)
            ggttataTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttta                                                                                                                                 <  1:148181/135‑1 (MQ=255)
            gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATTACAGTCAGgttta                                                                                                                                 >  2:762716/1‑141 (MQ=255)
                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                                                                                             >  2:505637/1‑141 (MQ=255)
                aaaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                                                                                             >  2:778331/1‑141 (MQ=255)
                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                                                                                            >  1:889344/1‑141 (MQ=255)
                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                                                                                           >  2:746368/1‑141 (MQ=255)
                  aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                                                                                                           <  2:874446/141‑1 (MQ=255)
                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                           >  2:537137/1‑141 (MQ=255)
                   tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGATAGTAAAgattaagtttaa                                                                                                                          >  1:729983/1‑141 (MQ=14)
                   tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                                                                                          >  1:798873/1‑141 (MQ=255)
                   tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                                                                                          >  2:598322/1‑141 (MQ=255)
                   tACATTCAATACCTATTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAACGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                                                                                          >  2:725374/1‑141 (MQ=255)
                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  2:935748/1‑141 (MQ=255)
                     cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                                                                                        >  2:562848/4‑141 (MQ=255)
                     cATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTaaaa                                                                                                                        <  2:552189/141‑1 (MQ=255)
                       ttCAATACCTTTTAAATTGTTCTTTGCCGTACTGATACCTTGATCACCTGGCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAGGTGGTTATTCAGATATTTCGATGTACAATTACAGTCAGGTTTAAGGTTAAAAGc                                                                                                                      <  2:322422/141‑1 (MQ=11)
                          aaTACCCTTTAAAGTATTCTTTCCCCTATTGATACTTTGAACTCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTCAGTTTAAAAGCttt                                                                                                                   <  1:403314/141‑1 (MQ=14)
                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGATTTAAt                                                                                                                >  1:94960/1‑141 (MQ=255)
                               cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTCATTCAGATATATCCATGTACAATGGCCGTCCAGTTTAAATTTAAAAGCTTTAAtaa                                                                                                              >  2:615145/1‑139 (MQ=14)
                                ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAttcc                                                                                                             >  1:47204/1‑139 (MQ=255)
                                   tAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAACAGCTTTAATTAtttt                                                                                                          >  2:689585/1‑141 (MQ=255)
                                    aaaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCCTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa                                                                                                         <  1:873841/141‑1 (MQ=255)
                                     aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaa                                                                                                        >  2:106448/1‑140 (MQ=255)
                                         aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                                                                                                    <  1:298340/141‑1 (MQ=255)
                                         aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                                                                                                    >  1:102052/1‑141 (MQ=255)
                                          ttttttGCTGTATTGATAATTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAtt                                                                                                   <  1:308768/138‑1 (MQ=255)
                                           tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCGGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg                                                                                                  <  2:1045915/141‑1 (MQ=255)
                                           tCTTTGCCGTCTTGAGTCTTTGCTATCTTTTCTGTCCTACTTCAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                                                                                                  <  2:79121/141‑1 (MQ=14)
                                              ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa                                                                                               >  1:163438/1‑141 (MQ=255)
                                               tgccgTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCtgc                                                                                              <  1:674150/137‑3 (MQ=255)
                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                            <  2:818582/141‑1 (MQ=255)
                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                            >  1:709902/1‑141 (MQ=255)
                                                   gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAACTTc                                                                                          >  2:48744/1‑141 (MQ=255)
                                                   gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTGAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTc                                                                                          >  2:671470/1‑141 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                        >  2:286726/1‑141 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                        <  2:95087/141‑1 (MQ=255)
                                                          tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAGGTTTAAAAGCTTTAATTCCTTTAACCATTGCTACCTTCGTTGAAg                                                                                   >  1:252572/1‑141 (MQ=255)
                                                          tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGGGATCTTGCTCAATGAGTTTATTCCGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAGAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAg                                                                                   <  1:554466/141‑1 (MQ=255)
                                                           acgttGATATCTTGTCTTTCTTACTGTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAgg                                                                                  <  2:271669/138‑1 (MQ=11)
                                                            cTTTGATTCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt                                                                                 <  2:24773/141‑1 (MQ=255)
                                                            cTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCCCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGt                                                                                 <  1:1048918/141‑1 (MQ=255)
                                                             tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTg                                                                                <  1:820408/141‑1 (MQ=38)
                                                                gATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCATATATTTCGTTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCt                                                                             >  1:656645/1‑141 (MQ=14)
                                                                gATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCt                                                                             >  1:493875/1‑141 (MQ=255)
                                                                 atatCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTg                                                                            >  1:96666/1‑141 (MQ=255)
                                                                     cTTGTCTTTCTTACTTTAATANGNCGGNGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTTAAGGTGCCTGATCt                                                                        <  1:568597/141‑1 (MQ=255)
                                                                      ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTg                                                                       >  1:22690/1‑141 (MQ=255)
                                                                      ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGACATTGATGCCTTCGTTGAAGGTGCCTGATCTg                                                                       >  2:962498/1‑141 (MQ=255)
                                                                             tcttACTTTAAGATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                <  1:51167/141‑1 (MQ=255)
                                                                              cttACTTTAATATGCCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAcc                                                               >  1:693845/1‑141 (MQ=255)
                                                                               ttACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                              <  1:582622/141‑1 (MQ=255)
                                                                                tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAccgt                                                             <  2:917148/141‑3 (MQ=255)
                                                                                 aCTTTAATAGGACGGTGATCTTGCTCAATTGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                            <  1:124451/141‑1 (MQ=11)
                                                                                        tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                     >  1:207735/1‑141 (MQ=255)
                                                                                         atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGttt                                                    >  1:904308/1‑141 (MQ=25)
                                                                                         atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTAAGGtgt                                                    >  2:360209/1‑139 (MQ=255)
                                                                                          tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                   >  1:1000204/1‑141 (MQ=255)
                                                                                           gACGGTGATAATGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTCATCTGTAACTACCTTTTGAGGCTTAc                                                  >  1:778529/1‑141 (MQ=11)
                                                                                            gcGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTTCAATTACCTTTTGAGGTTTAcc                                                 <  2:229659/140‑1 (MQ=11)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTTTTCTGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 <  1:984742/141‑1 (MQ=255)
                                                                                            aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTATTTACCTTTTGAGGTTTAcc                                                 >  1:862093/1‑141 (MQ=255)
                                                                                              ggTGATCTTGCTCAATGATGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATCGCTACCCTCGTTGAAGTTGCCTGATCTGTAATTACCTGTTGAGGTTTACCaa                                               <  1:378025/141‑1 (MQ=11)
                                                                                                tGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAt                                             <  2:385290/141‑1 (MQ=17)
                                                                                                 gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                            <  1:432651/141‑1 (MQ=17)
                                                                                                 gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGATTACCAAAtt                                            >  1:277242/1‑141 (MQ=255)
                                                                                                  aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGGTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                           >  2:1020197/1‑141 (MQ=12)
                                                                                                  aTCTGGCTCAAGGAGGTTATTCAGATATTTCGATGCACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                           <  1:106371/141‑1 (MQ=11)
                                                                                                   tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                          >  2:64714/1‑141 (MQ=12)
                                                                                                   tCTTGCTCAATGAGGATATTCAGATATTTCGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                          >  1:533755/1‑141 (MQ=255)
                                                                                                    cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATGGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTTTTGACGTTTACTAAATTGtt                                         >  2:1058718/1‑141 (MQ=11)
                                                                                                    cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                         >  2:1003288/1‑141 (MQ=11)
                                                                                                    cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                         >  1:916575/1‑141 (MQ=255)
                                                                                                       ccTCAATGAAGTTATTCAGATAATTCGATGTACAATGACAGTCAGGTCTAAGTTTAAAAGCTTTAACTATTTTAGCCATTTCTGCCCTCGTTGAAGGAGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                      <  2:195171/140‑1 (MQ=255)
                                                                                                        ctacaTGAGGTTATGCAAATATTTCGATGTACAATGACAGTCAGTGTTAAGACTAAATGATTTAATTACTTGAGCCATTGCTACGTTCGTTGAAGGTGCCTGATCCATAATTACATTTTGAGGTTTACCAAATTGTTTAAt                                     <  2:891641/137‑1 (MQ=11)
                                                                                                        cTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAAt                                     <  2:468672/141‑1 (MQ=11)
                                                                                                        cTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCCACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                     <  1:624365/141‑1 (MQ=255)
                                                                                                         tCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTCATg                                    <  1:763634/141‑1 (MQ=12)
                                                                                                         tCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGCTGCCTGATCTGTAATTACCTTTTGATGTTTACCAAATTGTTTAATg                                    <  1:746339/141‑1 (MQ=17)
                                                                                                           aaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                  >  2:633699/1‑141 (MQ=12)
                                                                                                            aTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgaga                                 >  1:1033355/1‑141 (MQ=11)
                                                                                                               aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                              >  2:421769/1‑141 (MQ=17)
                                                                                                                ggtgagtcaagtagttCGATGGACAATGACAGGCAGGTTTAAGCGTAAAAGCTTTAATGATTGGAGCCATTGCTGCCTTCGGTGAAGGTGCCTGATCTGTAACTTCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                             <  2:14525/127‑1 (MQ=11)
                                                                                                                  ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTGCCAAATTGTTTAATGAGACGTTTg                           >  2:577275/1‑141 (MQ=17)
                                                                                                                  ttATTCAGATATTTCGATGTACAAGGACAGTCAGTTTTCAGTTTAAAAGCTTGCATTATTTTAGGCATTGCTGCCTTCGTTGAAGGAGCATCATCTGTTAATCCCTTTTGAGGCTTACCAAATTGCTTAATGAGACGTTTg                           <  2:438200/141‑1 (MQ=11)
                                                                                                                   tataCAGATATTTCGATATACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGTAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                          <  2:710758/141‑1 (MQ=11)
                                                                                                                       cAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTACCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaaa                      >  1:338026/1‑141 (MQ=11)
                                                                                                                        aGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATaaaa                     >  2:853131/1‑140 (MQ=12)
                                                                                                                         gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                    <  2:125584/141‑1 (MQ=17)
                                                                                                                              ttCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat               <  1:847725/141‑1 (MQ=11)
                                                                                                                               tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatatt              >  1:18510/1‑140 (MQ=17)
                                                                                                                               tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              >  1:447160/1‑141 (MQ=12)
                                                                                                                               tCGATGTACAATGGCAGGCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATGGTTTAATGAGACGTCTGGTAGACGAATATg              >  2:164981/1‑141 (MQ=11)
                                                                                                                                cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc             <  2:303199/141‑1 (MQ=17)
                                                                                                                                cGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc             <  2:208383/141‑1 (MQ=17)
                                                                                                                                cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc             >  2:979288/1‑141 (MQ=11)
                                                                                                                                     tagaagaaCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt        <  2:103358/134‑1 (MQ=11)
                                                                                                                                      aaagtGACGGTGAGGTTTAACTTTACACGCGTTAATCATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCCGATCGGCAACTGCCTTTTCAGCCGTACCAAATTGTTGAATGAGACGTTTGATAAACGCCTATGCTGaagg       <  2:341593/137‑3 (MQ=2)
                                                                                                                                        aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt     <  1:603907/141‑1 (MQ=11)
                                                                                                                                            |                                                                                                                                           
TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: