Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,174 T→C 30.2% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1740TC30.2% 754.9 / inf 783K126K (AAA→AAGUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (258/282);  new base C (98/139);  total (358/425)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 803 total aligned reads displayed.

ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314
                                                                                                                                            |                                                                                                                                            
  atGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTAAGGtgt                                                                                                                                            >  2:360209/1‑139 (MQ=255)
  atGACGGTGATCTTGCCCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGttt                                                                                                                                            >  1:755970/1‑141 (MQ=255)
   tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                                                           >  1:1000204/1‑141 (MQ=255)
     aCGGTGATCTTGCTCGATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTGACTTTTGAGGCTTAcc                                                                                                                                         >  1:789667/1‑141 (MQ=11)
     aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                         <  2:384158/141‑1 (MQ=21)
          taTCTTGCTCAACGAGGTTAGTCAGACATTTCGATGCACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGCTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAtt                                                                                                                                    <  1:19778/140‑1 (MQ=11)
          gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                                    <  1:13685/141‑1 (MQ=17)
           aTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTATAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                                   >  2:768354/1‑141 (MQ=12)
           aTCTGGCTCAAGGAGGTTATTCAGATATTTCGATGCACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                                                                                                                   <  1:106371/141‑1 (MQ=11)
            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                  >  2:84498/1‑141 (MQ=12)
            tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACATTTTGAGGCTTACTAAATTGt                                                                                                                                  >  2:670499/1‑141 (MQ=11)
             cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCCAATtttt                                                                                                                                 >  1:435300/1‑141 (MQ=255)
               tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                                                                                                               <  1:152160/141‑1 (MQ=17)
                 ctacaTGAGGTTATGCAAATATTTCGATGTACAATGACAGTCAGTGTTAAGACTAAATGATTTAATTACTTGAGCCATTGCTACGTTCGTTGAAGGTGCCTGATCCATAATTACATTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                             <  2:891641/137‑1 (MQ=11)
                 cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                             <  1:58730/141‑1 (MQ=17)
                 cTCAATGAGGTTATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCCACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                             <  1:624365/141‑1 (MQ=255)
                   agatcgtggtATTCAGATATTTAGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                                           >  2:803273/10‑141 (MQ=11)
                     aTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgaga                                                                                                                         >  1:1033355/1‑141 (MQ=11)
                      tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                                                                                                                        >  1:511886/1‑141 (MQ=17)
                        gggTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                      <  2:138558/140‑1 (MQ=12)
                          gTTATTCATATATTTCTATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                    <  2:412516/141‑1 (MQ=255)
                          gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTCATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCTTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATTAGACGttt                                                                                                                    <  2:783742/141‑1 (MQ=11)
                          gTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGATTTACAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGttt                                                                                                                    <  1:605458/141‑1 (MQ=11)
                            tataCAGATATTTCGATATACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGTAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                  <  2:710758/141‑1 (MQ=11)
                            tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                  >  2:105657/1‑141 (MQ=12)
                            tATTCAGATATTTCGATGTACAATGACAGTCAGCTTTAAGTTTAAAAGCTTTACTGATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAACTTGTTTAATGAGACGTTTGa                                                                                                                  <  2:580959/141‑1 (MQ=11)
                                cAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                              >  1:227752/1‑141 (MQ=255)
                                cAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTACCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaaa                                                                                                              >  1:338026/1‑141 (MQ=11)
                                  gATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                                            <  1:472044/141‑1 (MQ=17)
                                     atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                                         <  1:689178/141‑1 (MQ=11)
                                      tttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata                                                                                                        >  2:184884/1‑141 (MQ=12)
                                      ttacGATGTACAGTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTCCCTGTTCTGTAACTACCTTTTGACGGCATCCAAATTGTTTAATGAGACGTTTGATAAACGCata                                                                                                        <  1:1047328/138‑1 (MQ=11)
                                       ttCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                                       <  1:847725/141‑1 (MQ=11)
                                        tctaTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                                      <  2:215303/138‑1 (MQ=12)
                                        tCGATGTATTATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAGTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                                      <  2:560885/141‑1 (MQ=11)
                                        tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGGGCCGTTTGGTAAACGCATATg                                                                                                      >  1:447177/1‑141 (MQ=11)
                                        tCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatatt                                                                                                      >  1:18510/1‑140 (MQ=17)
                                        tCGATGTACAAGGACAGTTAGGTTTCAGTTTAACAGCTTTAATTACTTTGGCCATTGCTACCTTCGTTGCAGGTGCCCGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATATTCGCATATg                                                                                                      <  2:234799/141‑1 (MQ=11)
                                             gTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                                 <  2:853514/141‑1 (MQ=32)
                                               agaacgACAGTCAGGTTTAAGTTTAAAAGACTGAATTAGTTTAGACATTGCTGCCAGAGTTGACGGTGCATGAACTGAATCTGACGTTTGAGGCATACCAAATTGTTTACTGAGACGATTTAGAAACGCACATGCTGAAtt                                                                                               >  2:154369/6‑140 (MQ=2)
                                                 aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                             >  2:964186/1‑141 (MQ=2)
                                                 aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTCATGGTACGTTTGATAAACGCATATGCTGAATGAt                                                                                             <  2:9505/141‑1 (MQ=11)
                                                    gACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt                                                                                          <  2:1033355/141‑1 (MQ=11)
                                                                     ttaagatctgttaTTGCTTTACCGAATGCTGCCTTGGTTGAAGGTGCCTGACCGGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGATACGTTTGATAGACGGATAGGCTGTATGATTGGCGCGTTGCTTACGCAAc                                                                         <  2:741217/129‑1 (MQ=0)
                                                                                   tattttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgt                                                           <  2:303003/138‑1 (MQ=0)
                                                                                      tttcccccttgttgccTTCGTTCAAGGTGCCTGATCTGTAACGCCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTGTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                        <  1:359878/127‑1 (MQ=11)
                                                                                      tttAACCATTGCTACCTTCGTTGAAGGTGCGTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                        >  2:253983/1‑141 (MQ=11)
                                                                                        taaccATTGCTACCTTCGTTGAAGGTGGCTAATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc                                                      <  1:286726/138‑1 (MQ=255)
                                                                                        tAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc                                                      >  2:612727/1‑141 (MQ=12)
                                                                                         aacaaTTGCTACCTTCGTTGAAGGTGCCTAATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  1:392549/137‑1 (MQ=17)
                                                                                         aGCCCTTGCTGTCTTCGTTAAACGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCACATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTcc                                                     <  1:546519/141‑1 (MQ=11)
                                                                                         aGCCATTGCTGCATTCGTTGAAGGTGACTGAACTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                     <  1:302285/141‑1 (MQ=11)
                                                                                          gCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                                    <  2:654871/141‑1 (MQ=12)
                                                                                           ccatctcTACCGTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTATCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct                                                   <  2:536921/135‑1 (MQ=11)
                                                                                           ccATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcccc                                                   >  1:379554/1‑140 (MQ=255)
                                                                                           ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct                                                   <  2:873238/141‑1 (MQ=32)
                                                                                             aTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctct                                                 <  1:652031/141‑1 (MQ=12)
                                                                                             aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCCTACGCAACCAAATATCTAATGTATGTCCccct                                                 >  2:54976/1‑141 (MQ=17)
                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                                <  1:712012/141‑1 (MQ=32)
                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                                >  2:1045297/1‑141 (MQ=32)
                                                                                               tGCTACCTTCGTTGCAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                               >  2:721451/1‑141 (MQ=17)
                                                                                               tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAGTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCCGc                                               >  2:1020430/1‑141 (MQ=17)
                                                                                                 ctgtcTTCGTTGAAGGTGCCTGATCTGTAACGACCTTTTGAGGCTTACCAAATTGTTTAGTGGGACGTTTGATAAACGCATATGCTGATTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                             <  1:531214/137‑1 (MQ=11)
                                                                                                  tACCTTCGTTGAAGGTGCCTGATCTGTATTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                            <  2:923220/141‑1 (MQ=17)
                                                                                                  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                            <  1:890067/141‑1 (MQ=17)
                                                                                                  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                            >  2:455328/1‑141 (MQ=32)
                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                           <  2:200479/141‑1 (MQ=17)
                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                           >  2:108120/1‑141 (MQ=32)
                                                                                                   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                           <  1:878467/141‑1 (MQ=17)
                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                          <  2:283365/141‑1 (MQ=12)
                                                                                                     cTTCGTTGAAGGTGCCTGTTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAAt                                         <  2:75663/141‑1 (MQ=17)
                                                                                                      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                        <  1:963442/141‑1 (MQ=17)
                                                                                                        tgTTTAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATACACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGc                                      <  1:777814/140‑1 (MQ=255)
                                                                                                         tttGATGGGGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGGATAGGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                     <  2:640559/140‑1 (MQ=11)
                                                                                                          ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                    <  2:437603/141‑1 (MQ=17)
                                                                                                          ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCAc                                    >  2:981196/1‑141 (MQ=17)
                                                                                                          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                    >  1:805548/1‑141 (MQ=12)
                                                                                                          ttGAAGGTGCCTGATATGTCCTGACGTTTTTCGGTTTAACAAATTGTTTAATAAGACGTTTGTTAAACTCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTTTGTCCGTCTGCATCAATGGccc                                    >  1:255275/1‑139 (MQ=11)
                                                                                                           tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                                   >  1:859748/1‑141 (MQ=32)
                                                                                                             aaGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGat                                 >  2:337585/1‑141 (MQ=17)
                                                                                                                     tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg                         >  1:415527/1‑141 (MQ=12)
                                                                                                                      gATCTGATATTACCTTTTGAGGTTTACCAAATTGTTTAATGATACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                        <  1:316379/141‑1 (MQ=255)
                                                                                                                         ttGTAACTACCTTTTGAGGTCTACCAAATTGTTTATTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                     <  1:166561/140‑1 (MQ=255)
                                                                                                                         cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                     >  1:738573/1‑141 (MQ=17)
                                                                                                                         cTGTAACTACCTTTTCAGGCTTACCCAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                     <  2:309800/141‑1 (MQ=11)
                                                                                                                            taactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAACACGCTCCAtt                  >  1:490074/5‑141 (MQ=11)
                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                  >  2:49450/1‑141 (MQ=17)
                                                                                                                            tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtt                  >  2:908675/1‑141 (MQ=32)
                                                                                                                               ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAAGATCTAATTTATGTACCTCTGCATCATTTGTCTGATATAAAAAGCTCCATTTTc               >  2:690573/1‑141 (MQ=11)
                                                                                                                               ttACCTTTTGAGGCGTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc               <  1:904881/141‑1 (MQ=255)
                                                                                                                                tACCTTTTGAGTCTTACCAAGTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc              <  1:639170/141‑1 (MQ=11)
                                                                                                                                 aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt             >  2:934910/1‑141 (MQ=17)
                                                                                                                                 aCCTTTTGAGGCTTTCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt             <  1:928108/141‑1 (MQ=12)
                                                                                                                                  ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt            <  1:992973/141‑1 (MQ=17)
                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt           >  1:273165/1‑141 (MQ=17)
                                                                                                                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt           <  1:564027/141‑1 (MQ=32)
                                                                                                                                    ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACTCATATGCTGAATGATTATCTCTTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCTCGACCTAAATAGCACCATTTTcatctt          >  1:101982/1‑136 (MQ=11)
                                                                                                                                    ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGTAACCAAATAAATAAGGAAAGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt          >  1:460372/1‑141 (MQ=11)
                                                                                                                                    ctaagAGGTGTCCCAAACTGTTTAATGAAACATTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt          <  1:11068/137‑1 (MQ=11)
                                                                                                                                     tttGAGGTTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa         <  1:211787/141‑1 (MQ=17)
                                                                                                                                            |                                                                                                                                            
ATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAT  >  CP000730/36034‑36314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: