Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,269 C→T 58.9% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,2690CT58.9% 268.9 / inf 975A95T (GCA→ACA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (195/205);  new base T (274/291);  total (473/502)
Fisher's exact test for biased strand distribution p-value = 9.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 986 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409
                                                                                                                                            |                                                                                                                                            
  cTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTGAGGTTTACCAAGTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTACCTCTGTAt                                                                                                                                            >  2:513563/1‑141 (MQ=11)
    aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  2:200479/141‑1 (MQ=17)
           tttaaGGTGCCTGATCTGTAATTACCTTTTGAGGTTGTCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTTTGCATCAATGGCAc                                                                                                                                   <  1:588547/138‑1 (MQ=255)
            tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCTTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                                                                                                                                  >  1:442966/1‑141 (MQ=17)
               aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                                                                                                                               >  1:47477/1‑141 (MQ=17)
               aGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAAGTTTGATAAACGCATATGCTGAATGATTATCACGTTGCTTACGCAACCAAGTAGCTAATGTATGTCCCTCTGTATCAAAGACACGatt                                                                                                                               >  2:304050/1‑140 (MQ=11)
                ggTGCCTGATCTGTAATTACGTTTTGAGATTTACCCAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACgccat                                                                                                                              >  2:886491/1‑137 (MQ=11)
                  tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATaa                                                                                                                            >  2:1016137/1‑141 (MQ=32)
                   gCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATTTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATTTaaa                                                                                                                           >  1:131461/1‑141 (MQ=11)
                       gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                                                                                                                       >  1:340794/1‑141 (MQ=32)
                        aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                                                                                                                      <  2:174427/141‑1 (MQ=17)
                        aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCt                                                                                                                      <  2:914766/141‑1 (MQ=32)
                         acTGGAACTACTTTCTGAGGCTTACCATAGCGTTTAATGAGACGGTTGATAAACGCATATGCTGAACGAATATCTCGGTGTGTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                     <  1:743709/140‑1 (MQ=11)
                          ttGTAACTACCTTTTGAGGTCTACCAAATTGTTTATTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                                                                                                                    <  1:166561/140‑1 (MQ=255)
                          atGTAATGAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCACATATCTAACATATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                                                                                                                    <  1:149793/140‑1 (MQ=11)
                             taactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAACACGCTCCAtt                                                                                                                 >  1:490074/5‑141 (MQ=11)
                               actACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                               >  2:1047136/3‑141 (MQ=12)
                                ctACCTTCTGAGGGTTACCAAATTGTTTAATGAGACGTTTGTTAAACGCGTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              <  1:459524/140‑1 (MQ=11)
                                  atctttgggggcgtACCACATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            <  2:130278/128‑1 (MQ=11)
                                  aCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            >  1:598649/1‑141 (MQ=17)
                                   ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTAATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAACAAATGTATGTCCCTCTGCATCACTGGCACGCTATAAAACGCTCCATTTTCCtt                                                                                                           >  1:1000901/1‑141 (MQ=11)
                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          >  1:273165/1‑141 (MQ=17)
                                     ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATCAATAGCTCCATTTTACtttt                                                                                                         >  2:1058851/1‑141 (MQ=17)
                                     tgttGAGGGTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCCGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                         <  1:383121/139‑1 (MQ=12)
                                     ctaagAGGTGTCCCAAACTGTTTAATGAAACATTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                         <  1:11068/137‑1 (MQ=11)
                                      tttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa                                                                                                        >  2:905308/1‑141 (MQ=32)
                                       ttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt                                                                                                       <  1:846191/141‑1 (MQ=17)
                                        tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      >  2:976630/1‑141 (MQ=17)
                                        tGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      >  1:293672/1‑141 (MQ=17)
                                        tGAGGCTTACCAAAGTGTTTAATGAGAGGTTTGATAAACGCATATGCTTAATGAGTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      <  1:260186/141‑1 (MQ=11)
                                           ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   >  1:973817/1‑141 (MQ=32)
                                            gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa                                                                                                  >  1:788537/1‑141 (MQ=17)
                                            gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa                                                                                                  <  1:925213/141‑1 (MQ=32)
                                             tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTCTTATTTTGAt                                                                                                 >  2:550394/1‑141 (MQ=17)
                                             tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTCCCTTTTATTTTGAt                                                                                                 <  1:554213/141‑1 (MQ=17)
                                              ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTCTCTAGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                >  2:484271/1‑141 (MQ=17)
                                               tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                               <  2:816755/141‑3 (MQ=17)
                                                aacAAATTGTTTAATGAGACGTCTGATAAACGCATTCGCAGAACGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCTGCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtata                                                                                              <  1:615256/139‑4 (MQ=11)
                                                    aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                          >  2:712820/1‑141 (MQ=12)
                                                    aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                          >  2:601931/1‑141 (MQ=17)
                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTATGtct                                                                                         >  1:82333/1‑141 (MQ=17)
                                                        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                      >  2:662215/1‑141 (MQ=17)
                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                  <  2:629137/141‑1 (MQ=12)
                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATCTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                 >  2:846625/1‑141 (MQ=12)
                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                >  2:953806/1‑141 (MQ=17)
                                                                   cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAt                                                                           <  1:633699/141‑1 (MQ=12)
                                                                     tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTATTGTATGTCTCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTGCTCGTCAATACGctcttt                                                                         >  1:52608/1‑136 (MQ=11)
                                                                     tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAttt                                                                         <  2:160487/141‑1 (MQ=17)
                                                                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCActtt                                                                        >  1:431524/1‑140 (MQ=12)
                                                                      ctgttgaccgcgTATGCTGAATGGTTATTGCGGTGCTTACGCTAACAAATAACTAATGTATGTCCCATTGTATGAACGGCACTATATAAATAGCCCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                        <  2:260574/133‑1 (MQ=2)
                                                                       tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGt                                                                       >  1:1038910/1‑141 (MQ=17)
                                                                        gATAAACGCATATGCTGAATAATTATCTCGTTGCTTACGCAACCAAATATCTAACGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCATTTATTTTGATGTACGTCTCATCATTACGCCATTTGta                                                                      >  1:688189/1‑141 (MQ=255)
                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     >  2:138580/1‑141 (MQ=32)
                                                                          tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAGTTGtaaa                                                                    >  1:913293/1‑140 (MQ=17)
                                                                           aaaCGCATATGCTGAATGATTATCTCGGTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                   <  2:360333/141‑1 (MQ=18)
                                                                            aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                  >  2:56962/1‑141 (MQ=21)
                                                                               gCATATGCTTAATGATTGTCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGTATCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGACATTTGTAATAAGCt                                                               <  1:699408/141‑1 (MQ=11)
                                                                                 atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCAATGGCTCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttt                                                             >  1:726516/1‑141 (MQ=21)
                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            >  1:861248/1‑141 (MQ=21)
                                                                                   atGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                           <  2:673435/141‑1 (MQ=35)
                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  2:939368/1‑141 (MQ=25)
                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  1:214691/1‑141 (MQ=35)
                                                                                     gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                         <  2:1030292/141‑1 (MQ=25)
                                                                                      cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                        <  1:757212/141‑1 (MQ=38)
                                                                                       tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       <  1:19046/141‑1 (MQ=38)
                                                                                        taatctttgtctcGTTCCTTCCGCAACCAAATACCTAATTTATGTCCCTCTGTATCCATGCAACAATATAAATAGCGCCATTTTCCTTTTTTTTTTCTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTCTATGc                                                      <  2:761119/132‑1 (MQ=2)
                                                                                        gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCACTACGCCATTTGTAATAAGCGATTTTATGc                                                      >  1:361578/1‑141 (MQ=11)
                                                                                        gAATGATTATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                      <  1:573637/141‑1 (MQ=255)
                                                                                           tGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTCGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttt                                                   <  2:435300/141‑1 (MQ=255)
                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  >  2:389763/1‑141 (MQ=255)
                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  1:804015/1‑141 (MQ=255)
                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATCGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACACTACTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  2:702183/141‑1 (MQ=14)
                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTttctt                                              >  1:628028/1‑141 (MQ=255)
                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  1:1058718/141‑1 (MQ=40)
                                                                                                   tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCa                                           <  2:805548/141‑1 (MQ=255)
                                                                                                       tccTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                       <  2:69187/139‑1 (MQ=255)
                                                                                                        gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAttt                                      >  2:778795/1‑141 (MQ=255)
                                                                                                        gCTTACGCAACCAAATATCTAATGTAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAttt                                      <  1:322552/141‑1 (MQ=255)
                                                                                                            tcGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                  <  2:855256/140‑1 (MQ=40)
                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACCTCAATTTTAATAAAATTTTTTTTGCTTTATATTCCAAAtttatga                                  >  1:539144/1‑137 (MQ=14)
                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                  <  1:408317/141‑1 (MQ=40)
                                                                                                             cGCAATCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:881896/141‑2 (MQ=255)
                                                                                                             agCAACCAATGTTCTAATGTATGTCCCGCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  2:742884/140‑2 (MQ=255)
                                                                                                                aaCCAAATATCTAAGGTATGTCCCGCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                              <  2:455850/141‑1 (MQ=11)
                                                                                                                      atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAACTTggg                        >  1:510864/1‑141 (MQ=11)
                                                                                                                       tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       >  2:37919/1‑141 (MQ=255)
                                                                                                                          cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCat                    <  1:778795/141‑1 (MQ=255)
                                                                                                                          cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  1:670499/141‑1 (MQ=255)
                                                                                                                          cTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  1:956113/141‑1 (MQ=37)
                                                                                                                           tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAACTTGTGGCata                   >  2:217876/1‑141 (MQ=11)
                                                                                                                           tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  1:1045297/141‑1 (MQ=255)
                                                                                                                            aaTGTATGTTCCTCTTCATCAATGGAAAGATATAAATAGCTCAATTTTCCTTTTATTTTGATGTACGTCTCGTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCatag                  <  1:305602/141‑2 (MQ=14)
                                                                                                                            aaTGTAGTTCCCTCTGTATCAATGGCACGATAGAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                  <  1:226754/141‑1 (MQ=11)
                                                                                                                               gtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCTATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt               <  1:1020197/141‑1 (MQ=255)
                                                                                                                                  tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGa            >  2:338735/1‑141 (MQ=255)
                                                                                                                                     cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTTGGGCATATTCTTTAAcc         >  2:417839/1‑141 (MQ=11)
                                                                                                                                     cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATCAAATTGGGGCATATTCGTGAAcc         >  1:901425/1‑141 (MQ=255)
                                                                                                                                        tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATATAAAATTGGGTAATGTCCTTTAACCCaa      >  2:524745/1‑141 (MQ=11)
                                                                                                                                         ctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc     <  1:989967/141‑1 (MQ=255)
                                                                                                                                          tGCATCGATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg    >  2:586444/1‑141 (MQ=25)
                                                                                                                                            |                                                                                                                                            
TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: