Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,413 C→T 51.8% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,4130CT51.8% 34.0 / inf 551V47I (GTC→ATC) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (117/148);  new base T (138/146);  total (256/295)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

Only 100 of 562 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553
                                                                                                                                             |                                                                                                                                             
 aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCATCGGTAGAcc                                                                                                                                              >  2:1042739/1‑141 (MQ=255)
   ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTATCATACATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                                                            >  1:943585/1‑141 (MQ=11)
    gCACGATATACATAGCCTCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCGAATTTGACACAAAACTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                                                                                                                           <  2:967330/141‑1 (MQ=14)
     cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAGTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTAGAACCCTACGGTAGACCGTTg                                                                                                                                          <  2:726516/141‑1 (MQ=255)
     cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                                                          >  1:651303/1‑141 (MQ=255)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAACGGTAGACCGTTGa                                                                                                                                         >  1:3021/1‑141 (MQ=21)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCGTATTCTTGAACCCAACGGGAGACCGTGGa                                                                                                                                         >  1:1015455/1‑141 (MQ=255)
      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCACCGGTAGATCGTTGa                                                                                                                                         >  2:204202/1‑141 (MQ=11)
       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACACAACGGTAGACCGTTGaa                                                                                                                                        >  1:581429/1‑141 (MQ=255)
       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGAACCCAACGGTAGATCGtgtaa                                                                                                                                        >  2:673703/1‑137 (MQ=11)
        gATATAAATAGCTCCATTTTGATTTTATTTCGTTATGCAACTCTTCTATACGCATAATCTTTGAAGTTTTTTGTATCGTTTTCTGCCAAAGTTGAGATAAAATTTGTTCATATTCTTGAACCCAACGGTAGACCGTTGAat                                                                                                                                       <  2:883156/141‑1 (MQ=0)
        gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAat                                                                                                                                       >  2:352593/1‑141 (MQ=25)
         atatAAATTGCTCCTATTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                      <  1:260287/141‑1 (MQ=255)
         atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGCTAGACCGTTTAatg                                                                                                                                      >  1:48523/1‑141 (MQ=255)
         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGCCCGCTGAatg                                                                                                                                      <  2:1052065/141‑1 (MQ=11)
         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                      <  1:976630/141‑1 (MQ=255)
         atatAAATAGCTCCATTTTCATTTTATTGTGATGTACGTCTCATCAATACGCCATTTGTAATACGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTATTGAACCCAACGGTAGACCGATGAatt                                                                                                                                      >  2:387279/1‑140 (MQ=14)
          tataCATAGCTCCATTTTCCTTTTATTTTGATATCCGTCACATTAATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTTTCAATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                     <  2:561061/141‑1 (MQ=11)
            taAATAGCTCCATTTTCCTTTTATTTTGATATGCGCTTTATTAGTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                                                  <  2:479960/141‑1 (MQ=11)
              aaTATCTCCATTTTCCTTTTATTTTGATATTCGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                                <  2:173475/141‑1 (MQ=11)
              aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTCATCTTGAACCCAACGATAGACCGTTGAATGATGaa                                                                                                                                >  1:743428/1‑141 (MQ=11)
               aTAGCTCCATTTTCCTTTTACTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                                                                                                                               >  1:662986/1‑141 (MQ=255)
                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                                                                        >  1:752787/1‑141 (MQ=21)
                      cATTTTCCTTTTATTTTGAAATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                        >  1:791400/1‑141 (MQ=255)
                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                                                       >  2:523637/1‑141 (MQ=255)
                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                                                                       <  1:209894/141‑1 (MQ=255)
                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  2:119460/141‑1 (MQ=255)
                         ttgccgtttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  2:631722/135‑1 (MQ=255)
                           tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                                                                   <  1:1022395/141‑1 (MQ=39)
                           tCCTTTTATTTTGACGTACTTCTCATCAATACGCCATTTGTAATAAGCTTTTTTACGCCTTTTCTTCCAAATTTCATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGTCCGTTGAATGACGAACGTTTACACCACg                                                                                                                   <  2:547353/141‑1 (MQ=11)
                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                            <  2:693318/131‑1 (MQ=255)
                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                                  <  1:56327/141‑1 (MQ=255)
                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                                  <  2:455963/141‑1 (MQ=255)
                             cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 >  1:613239/1‑141 (MQ=21)
                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 >  1:473547/1‑141 (MQ=255)
                                 tATTTTGCTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                                             <  2:371483/141‑4 (MQ=11)
                                 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                             <  1:716418/141‑1 (MQ=25)
                                  aTTTCGATGTACGTCTCCTCAATACGCCTTTTGTAATACGCTTTTTTATGCTTTTTCTTCGAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                                            <  1:450505/141‑1 (MQ=11)
                                   ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                           <  2:742695/141‑1 (MQ=11)
                                   ttctGATTTACGTCTCATCAATACGCTATTTTTAATAAGCTTTTTTATGCGTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCTTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                                           <  1:830307/138‑1 (MQ=11)
                                    tttGATGTACCTCTCATCAATACGCCACTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGCGCATATTCTTGAACCCAACCGTAGACCGTTGAATTATGAACGTTTACACCACGTTCCCTTaa                                                                                                          <  2:808891/141‑1 (MQ=14)
                                    ttcgttatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                          <  2:30920/134‑1 (MQ=11)
                                     ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                                                         <  2:62173/141‑1 (MQ=21)
                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAGCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTCatat                                                                                                       >  2:418027/5‑141 (MQ=11)
                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAGGGCATATTCTTGAACCCAACGTTAGATCGTTGAATGATGAACGTTTACAACACGTTCTCTTAatat                                                                                                       >  1:156234/5‑141 (MQ=11)
                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                       >  1:847029/1‑141 (MQ=255)
                                          gTACGTCTCATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTTTGTTACATCAGGGATATAGAATAGGGGCGTATTCTTGAACCCAACGGTAGACGGTTGAATGATGAACGTTGACACCACGGTCCCTTAATATTTc                                                                                                    >  1:775959/1‑141 (MQ=11)
                                           tACCTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                   <  1:54636/141‑1 (MQ=255)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTATACAGTCGATGATACAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                                 <  1:509585/141‑1 (MQ=11)
                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCCCTTCATATTTCAgc                                                                                                 >  2:13034/1‑140 (MQ=255)
                                                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                              <  1:954303/141‑1 (MQ=255)
                                                ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                              <  2:793880/141‑1 (MQ=255)
                                                 tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                                             >  1:345972/1‑141 (MQ=255)
                                                 tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                                             <  1:556694/141‑1 (MQ=25)
                                                  catcaATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCATGTTCTCTTAATATTTCCGatatat                                                                                            <  1:674521/141‑1 (MQ=11)
                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                           <  1:382823/141‑1 (MQ=255)
                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                           <  1:283884/141‑1 (MQ=21)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCGGATATATCa                                                                                          >  2:186123/1‑141 (MQ=11)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAATATTCTTGAACCCAACGGTAGATCGATGAATGATGAAAGTTTACACCACGTTATCTTAATATTTCAGATAAATCa                                                                                          >  2:235375/1‑141 (MQ=11)
                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                          <  2:1017421/141‑1 (MQ=39)
                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         >  1:436535/1‑141 (MQ=18)
                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         >  1:889589/1‑141 (MQ=21)
                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         <  2:445703/141‑1 (MQ=40)
                                                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                        >  1:591956/1‑141 (MQ=255)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                       >  1:420394/1‑141 (MQ=255)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                       >  1:407502/1‑141 (MQ=255)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACATTTACACCACGTTCTCTTAATGTTTCAGATATATCACGa                                                                                       >  1:112365/1‑141 (MQ=11)
                                                       aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                       <  2:849154/141‑1 (MQ=18)
                                                          cGCCATTTGTAATAAGCTTTTTTTTTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCCGATATATCCCGATaa                                                                                    >  2:654033/1‑141 (MQ=11)
                                                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                    <  2:835772/141‑2 (MQ=255)
                                                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTATTAATATTTCAGATATATCACGATag                                                                                    >  1:640830/1‑140 (MQ=11)
                                                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                    <  2:861248/141‑1 (MQ=21)
                                                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                   <  2:278061/141‑1 (MQ=39)
                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCCCGTTGAATGATGACCGTTTACACCACGTACCCTTAATATTTCTGATATATCACGATaaac                                                                                  >  2:251514/1‑139 (MQ=11)
                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                  >  2:581411/1‑141 (MQ=255)
                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                  >  1:908149/1‑141 (MQ=255)
                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                  >  2:940466/1‑141 (MQ=25)
                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGACGATCGTTGAAAGATGACCGTTTACACCACCATCTCTTAAACTTTCAAAATTATCACGAtggct                                                                                  >  1:239280/1‑137 (MQ=11)
                                                             cATTTGTCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCAGGTCCCCTTACTGTTTCTGATATATCACGATCACTc                                                                                 >  2:596130/1‑141 (MQ=11)
                                                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                >  1:338595/1‑141 (MQ=25)
                                                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                              >  1:985869/1‑141 (MQ=40)
                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                             <  2:325234/141‑1 (MQ=39)
                                                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                            <  1:1001540/141‑2 (MQ=11)
                                                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                            <  1:105569/141‑2 (MQ=11)
                                                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                           >  1:858045/1‑139 (MQ=11)
                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaagaca                                                                           >  2:193590/1‑137 (MQ=11)
                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTACACAACGATCTCTTAATATTTCAGATATATCACGATAGCTCaaaaca                                                                           >  2:897913/1‑137 (MQ=11)
                                                                    aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTgaatgaat                                                                          >  1:693710/1‑141 (MQ=11)
                                                                      taaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcctat                                                                        >  1:128654/1‑136 (MQ=0)
                                                                                  aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGTTAACTCAATGTATATCTTAGAtagta                                                             >  1:191467/1‑141 (MQ=25)
                                                                                        tttCCTCCAAATTTGATACAAAATTGGTGCTTATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGccgtt                                                       <  1:35865/141‑4 (MQ=255)
                                                                                                    ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAt                                           <  2:506800/141‑1 (MQ=35)
                                                                                                     tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                          >  1:228455/1‑141 (MQ=35)
                                                                                                              aTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAAGGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                 <  1:728073/141‑1 (MQ=17)
                                                                                                               ttGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                <  1:871889/141‑1 (MQ=17)
                                                                                                               ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                >  1:699401/1‑141 (MQ=32)
                                                                                                               ttGGGACATATTCTTTAACCCAACGCTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCCGATATATCACGTTGTCCCATTGTATGTCTGACATAGTATCCAACGGCTAGAGTGAGAACATCCTTGt                                <  2:40639/141‑1 (MQ=11)
                                                                                                                tGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                               <  2:777507/141‑1 (MQ=17)
                                                                                                                  gggCATATTCTTGAACCCAACGGCAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                             <  1:1035448/141‑1 (MQ=17)
                                                                                                                                 cccAACTGTAGACCGTTGAATGATTAACGTTTACACCACGTTCCCTTAATATTTCAGATATAGCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa              <  2:923171/141‑1 (MQ=12)
                                                                                                                                             |                                                                                                                                             
AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: