BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,413	C→T	51.8%	V47I (GTC→ATC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,413	0	C	T	51.8%	34.0 / inf	551	V47I (GTC→ATC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (117/148); new base T (138/146); total (256/295)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

Only 100 of 562 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553

 aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCATCGGTAGAcc                                                                                                                                              >  2:1042739/1‑141 (MQ=255)

   ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTATCATACATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                                                            >  1:943585/1‑141 (MQ=11)

    gCACGATATACATAGCCTCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCGAATTTGACACAAAACTGGGGCATATTCTTGAACCCAACGGTAGACCGtt                                                                                                                                           <  2:967330/141‑1 (MQ=14)

     cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAGTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTAGAACCCTACGGTAGACCGTTg                                                                                                                                          <  2:726516/141‑1 (MQ=255)

     cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                                                          >  1:651303/1‑141 (MQ=255)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAACGGTAGACCGTTGa                                                                                                                                         >  1:3021/1‑141 (MQ=21)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCGTATTCTTGAACCCAACGGGAGACCGTGGa                                                                                                                                         >  1:1015455/1‑141 (MQ=255)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCACCGGTAGATCGTTGa                                                                                                                                         >  2:204202/1‑141 (MQ=11)

       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACACAACGGTAGACCGTTGaa                                                                                                                                        >  1:581429/1‑141 (MQ=255)

       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGAACCCAACGGTAGATCGtgtaa                                                                                                                                        >  2:673703/1‑137 (MQ=11)

        gATATAAATAGCTCCATTTTGATTTTATTTCGTTATGCAACTCTTCTATACGCATAATCTTTGAAGTTTTTTGTAT‑CGTTTTCTGCCAAAGTTGAGATAAAATTTGTTCATATTCTTGAACCCAACGGTAGACCGTTGAat                                                                                                                                       <  2:883156/141‑1 (MQ=0)

        gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAat                                                                                                                                       >  2:352593/1‑141 (MQ=25)

         atatAAATTGCTCCTATTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                                                                      <  1:260287/141‑1 (MQ=255)

         atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGCTAGACCGTTTAatg                                                                                                                                      >  1:48523/1‑141 (MQ=255)

         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGCCCGCTGAatg                                                                                                                                      <  2:1052065/141‑1 (MQ=11)

         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                      <  1:976630/141‑1 (MQ=255)

         atatAAATAGCTCCATTTTCATTTTATTGTGATGTACGTCTCATCAATACGCCATTTGTAATACGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTATTGAACCCAACGGTAGACCGATGAatt                                                                                                                                      >  2:387279/1‑140 (MQ=14)

          tataCATAGCTCCATTTTCCTTTTATTTTGATATCCGTCACATTAATACGCCATTTGTAATAAGCTTTGT‑TATGCTTTTTCTTTCAATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                                                                                                                     <  2:561061/141‑1 (MQ=11)

            taAATAGCTCCATTTTCCTTTTATTTTGATATGCGCTTTATTAGTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat‑g                                                                                                                                  <  2:479960/141‑1 (MQ=11)

              aaTATCTCCATTTTCCTTTTATTTTGATATTCGGCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑Gaa                                                                                                                                <  2:173475/141‑1 (MQ=11)

              aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTCATCTTGAACCCAACGATAGACCGTTGAATGAT‑Gaa                                                                                                                                >  1:743428/1‑141 (MQ=11)

               aTAGCTCCATTTTCCTTTTACTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAAc                                                                                                                               >  1:662986/1‑141 (MQ=255)

                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTaca                                                                                                                        >  1:752787/1‑141 (MQ=21)

                      cATTTTCCTTTTATTTTGAAATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTaca                                                                                                                        >  1:791400/1‑141 (MQ=255)

                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATCTAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTacac                                                                                                                       >  2:523637/1‑141 (MQ=255)

                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTAGATCGTTGAATGAT‑GAACGTTTacac                                                                                                                       <  1:209894/141‑1 (MQ=255)

                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTAcacca                                                                                                                     <  2:119460/141‑1 (MQ=255)

                         ttgccgtttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTAcacca                                                                                                                     <  2:631722/135‑1 (MQ=255)

                           tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACg                                                                                                                   <  1:1022395/141‑1 (MQ=39)

                           tCCTTTTATTTTGACGTACTTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TACGCCTTTTCTTCCAAATTTCATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGTCCGTTGAATGAC‑GAACGTTTACACCACg                                                                                                                   <  2:547353/141‑1 (MQ=11)

                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGtt                                                                                                                            <  2:693318/131‑1 (MQ=255)

                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGt                                                                                                                  <  1:56327/141‑1 (MQ=255)

                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGt                                                                                                                  <  2:455963/141‑1 (MQ=255)

                             cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGtt                                                                                                                 >  1:613239/1‑141 (MQ=21)

                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGtt                                                                                                                 >  1:473547/1‑141 (MQ=255)

                                 tATTTTGCTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTctct                                                                                                             <  2:371483/141‑4 (MQ=11)

                                 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCt                                                                                                             <  1:716418/141‑1 (MQ=25)

                                  aTTTCGATGTACGTCTCCTCAATACGCCTTTTGTAATACGCTTTTT‑TATGCTTTTTCTTCGAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCtt                                                                                                            <  1:450505/141‑1 (MQ=11)

                                   ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTa                                                                                                           <  2:742695/141‑1 (MQ=11)

                                   ttctGATTTACGTCTCATCAATACGCTATTTTTAATAAGCTTTTT‑TATGCGTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCTTTGAATGAT‑GAACGTTTACACCACGTTCTCTTa                                                                                                           <  1:830307/138‑1 (MQ=11)

                                    tttGATGTACCTCTCATCAATACGCCACTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGCGCATATTCTTGAACCCAACCGTAGACCGTTGAATTAT‑GAACGTTTACACCACGTTCCCTTaa                                                                                                          <  2:808891/141‑1 (MQ=14)

                                    ttcgttatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTaa                                                                                                          <  2:30920/134‑1 (MQ=11)

                                     ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAat                                                                                                         <  2:62173/141‑1 (MQ=21)

                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAGCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTCatat                                                                                                       >  2:418027/5‑141 (MQ=11)

                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTAGGGCATATTCTTGAACCCAACGTTAGATCGTTGAATGAT‑GAACGTTTACAACACGTTCTCTTAatat                                                                                                       >  1:156234/5‑141 (MQ=11)

                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTTTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAatat                                                                                                       >  1:847029/1‑141 (MQ=255)

                                          gTACGTCTCATCAATACGCCATTTCTAATAAGCTTTTT‑TATGCTTTTTGTTACATCAGGGATATAGAATAGGGGCGTATTCTTGAACCCAACGGTAGACGGTTGAATGAT‑GAACGTTGACACCACGGTCCCTTAATATTTc                                                                                                    >  1:775959/1‑141 (MQ=11)

                                           tACCTCCCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                   <  1:54636/141‑1 (MQ=255)

                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTATACAGTCG‑ATGATACAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                                                 <  1:509585/141‑1 (MQ=11)

                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTCATATTTCAgc                                                                                                 >  2:13034/1‑140 (MQ=255)

                                                ctcATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                              <  1:954303/141‑1 (MQ=255)

                                                ctcATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                                              <  2:793880/141‑1 (MQ=255)

                                                 tcatcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                                             >  1:345972/1‑141 (MQ=255)

                                                 tcatcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                                             <  1:556694/141‑1 (MQ=25)

                                                  catcaATACGCCATTTGTAATAATCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCATGTTCTCTTAATATTTCCGatatat                                                                                            <  1:674521/141‑1 (MQ=11)

                                                   atcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                                                           <  1:382823/141‑1 (MQ=255)

                                                   atcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                           <  1:283884/141‑1 (MQ=21)

                                                    tcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCGGATATATCa                                                                                          >  2:186123/1‑141 (MQ=11)

                                                    tcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGAATATTCTTGAACCCAACGGTAGATCGATGAATGAT‑GAAAGTTTACACCACGTTATCTTAATATTTCAGATAAATCa                                                                                          >  2:235375/1‑141 (MQ=11)

                                                    tcaATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                          <  2:1017421/141‑1 (MQ=39)

                                                     caATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         >  1:436535/1‑141 (MQ=18)

                                                     caATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         >  1:889589/1‑141 (MQ=21)

                                                     caATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         <  2:445703/141‑1 (MQ=40)

                                                      aaTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                        >  1:591956/1‑141 (MQ=255)

                                                       aTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                       >  1:420394/1‑141 (MQ=255)

                                                       aTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                                                       >  1:407502/1‑141 (MQ=255)

                                                       aTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACATTTACACCACGTTCTCTTAATGTTTCAGATATATCACGa                                                                                       >  1:112365/1‑141 (MQ=11)

                                                       aTACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                       <  2:849154/141‑1 (MQ=18)

                                                          cGCCATTTGTAATAAGCTTTTT‑TTTTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCCGATATATCCCGATaa                                                                                    >  2:654033/1‑141 (MQ=11)

                                                          cGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                                                    <  2:835772/141‑2 (MQ=255)

                                                          cGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTATTAATATTTCAGATATATCACGATag                                                                                    >  1:640830/1‑140 (MQ=11)

                                                          cGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                    <  2:861248/141‑1 (MQ=21)

                                                           gCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                   <  2:278061/141‑1 (MQ=39)

                                                            ccATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCCCGTTGAATGAT‑GACCGTTTACACCACGTACCCTTAATATTTCTGATATATCACGATaaac                                                                                  >  2:251514/1‑139 (MQ=11)

                                                            ccATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                  >  2:581411/1‑141 (MQ=255)

                                                            ccATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                  >  1:908149/1‑141 (MQ=255)

                                                            ccATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                  >  2:940466/1‑141 (MQ=25)

                                                            ccATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGACGATCGTTGAAAGAT‑GACCGTTTACACCACCATCTCTTAAACTTTCAAAATTATCACGAtggct                                                                                  >  1:239280/1‑137 (MQ=11)

                                                             cATTTGTCATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCAGGTCCCCTTACTGTTTCTGATATATCACGATCACTc                                                                                 >  2:596130/1‑141 (MQ=11)

                                                              aTTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                >  1:338595/1‑141 (MQ=25)

                                                                ttGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                              >  1:985869/1‑141 (MQ=40)

                                                                 tGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                             <  2:325234/141‑1 (MQ=39)

                                                                  gTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                            <  1:1001540/141‑2 (MQ=11)

                                                                  gTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                            <  1:105569/141‑2 (MQ=11)

                                                                  gTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATA‑TAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                           >  1:858045/1‑139 (MQ=11)

                                                                   taataaGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaagaca                                                                           >  2:193590/1‑137 (MQ=11)

                                                                   taataaGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAAAGTTTACACAACGATCTCTTAATATTTCAGATATATCACGATAGCTCaaaaca                                                                           >  2:897913/1‑137 (MQ=11)

                                                                    aataaGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGAT‑GAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTgaatgaat                                                                          >  1:693710/1‑141 (MQ=11)

                                                                      taaGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgcctat                                                                        >  1:128654/1‑136 (MQ=0)

                                                                                  aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGTTAACTCAATGTATATCTTAGAtagta                                                             >  1:191467/1‑141 (MQ=25)

                                                                                        tttCCTCCAAATTTGATACAAAATTGGTGCTTATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGccgtt                                                       <  1:35865/141‑4 (MQ=255)

                                                                                                    ttGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAt                                           <  2:506800/141‑1 (MQ=35)

                                                                                                     tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                          >  1:228455/1‑141 (MQ=35)

                                                                                                              aTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTAAAGGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                 <  1:728073/141‑1 (MQ=17)

                                                                                                               ttGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                <  1:871889/141‑1 (MQ=17)

                                                                                                               ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                >  1:699401/1‑141 (MQ=32)

                                                                                                               ttGGGACATATTCTTTAACCCAACGCTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCCGATATATCACGTTGTCCCATTGTATGTCTGACATAGTATCCAACGGCTAGAGTGAGAACATCCTTGt                                <  2:40639/141‑1 (MQ=11)

                                                                                                                tGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGtt                               <  2:777507/141‑1 (MQ=17)

                                                                                                                  gggCATATTCTTGAACCCAACGGCAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                             <  1:1035448/141‑1 (MQ=17)

                                                                                                                                 cccAACTGTAGACCGTTGAATGAT‑TAACGTTTACACCACGTTCCCTTAATATTTCAGATATAGCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa              <  2:923171/141‑1 (MQ=12)

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTT‑TATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAT‑GAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`