Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 35.0% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT35.0% 194.6 / 130.9 151intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (51/47);  new base T (24/29);  total (75/76)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.59e-01

Only 100 of 152 total aligned reads displayed.

TTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTA  >  CP000730/36441‑36719
                                                                                                                                            |                                                                                                                                          
ttgccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAc                                                                                                                                            <  2:296580/138‑1 (MQ=17)
  cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTGTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTAATTTTTTACtt                                                                                                                                          >  2:703258/1‑140 (MQ=11)
              cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt                                                                                                                              >  2:188940/1‑141 (MQ=35)
              cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt                                                                                                                              >  2:323225/1‑141 (MQ=35)
              cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt                                                                                                                              >  1:344295/1‑141 (MQ=35)
                 atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                           >  1:275111/1‑141 (MQ=35)
                  tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                                                                                                          >  2:880522/1‑141 (MQ=35)
                   atatCACGATAGCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCaa                                                                                                                         >  1:156417/1‑141 (MQ=25)
                      tCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCACCAg                                                                                                                      >  2:644793/1‑141 (MQ=25)
                          gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACATAAGACTCTTTTTTGTTAAAATTATACTATAATTTCAACTTTGCAACAGAAcc                                                                                                                  <  1:646080/141‑1 (MQ=255)
                          gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcc                                                                                                                  <  1:418981/141‑1 (MQ=34)
                          gATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcc                                                                                                                  <  1:449144/141‑1 (MQ=35)
                            tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                <  1:427652/138‑1 (MQ=9)
                            tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                <  1:520365/138‑1 (MQ=9)
                              gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGCAATAGGTCATACCGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtct                                                                                                              >  2:519792/2‑139 (MQ=11)
                              gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTTCAACAGAACCGtat                                                                                                              >  2:345531/2‑141 (MQ=12)
                              gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                              <  1:1021151/140‑1 (MQ=16)
                              gcTCAATGCATATCTTAGATAGTAACCAAAGGCTACAGTGATAACATCCTTGTTAAATTGTATATATCTGAAATAGTTCATACAGAAGACTCATTTTTGTTAAAATTATATTGTAACTTCAACTTTGCGACAGAACCGtat                                                                                                              >  2:569962/2‑141 (MQ=11)
                               cacagtgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctccta                                                                                                             >  2:535934/9‑136 (MQ=11)
                               cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                             >  2:956179/1‑141 (MQ=16)
                               cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                             >  1:537002/1‑141 (MQ=16)
                               cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                             >  2:111099/1‑141 (MQ=16)
                               cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                             >  2:823542/1‑141 (MQ=16)
                                tCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                            >  1:1011714/1‑141 (MQ=40)
                                tCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                            >  1:73968/1‑141 (MQ=40)
                                tCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                            <  1:906165/141‑1 (MQ=40)
                                tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                            <  1:106745/141‑2 (MQ=16)
                                tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                            <  1:223217/141‑2 (MQ=12)
                                tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                            <  1:107569/141‑2 (MQ=19)
                                tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatgt                                                                                                            >  1:47710/1‑139 (MQ=12)
                                tCAATGCATATCTTAAATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                            >  1:787303/1‑140 (MQ=255)
                                 cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                                                                                                           <  2:192865/141‑3 (MQ=255)
                                 cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                                                                                                           <  1:575929/141‑3 (MQ=255)
                                  aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGTCTTTTg                                                                                                          >  1:1033368/1‑141 (MQ=255)
                                   agacatatCTTAGATCGAAGCCAACACCTAAAGTGATAACATCCAACTTCAATAGCTTCTCTCTAACCTACTTCCTACAGAAGACTCATTTCTGTTACCATTATACTATAAATTCAACTTTGCAACAGAAcctcatttact                                                                                                         >  2:129945/5‑132 (MQ=2)
                                    tgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTTAAATTTGCAACAGAATCGtattttatg                                                                                                        >  1:471347/4‑136 (MQ=11)
                                    tgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                        >  1:269761/4‑136 (MQ=255)
                                     gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                       >  2:456595/3‑135 (MQ=255)
                                      catatCTTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                      <  1:204202/140‑13 (MQ=11)
                                      catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                      <  1:576011/140‑13 (MQ=11)
                                      catatCGTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                      <  2:1036145/140‑8 (MQ=255)
                                       atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtttt                                                                                                     >  2:989078/1‑128 (MQ=11)
                                       atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                     <  1:1031578/141‑14 (MQ=11)
                                       atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTGTTGTTAACATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                     <  2:436535/141‑14 (MQ=11)
                                        tatCTTAGATAGTAGTCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                    <  2:958216/141‑10 (MQ=11)
                                        tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAACTTGTATATATCTGAAATCGTCCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCgccatctatgagta                                                                                                    >  2:874529/1‑128 (MQ=11)
                                        tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGCAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCATATTATGGAATag                                                                                                    >  2:598639/1‑141 (MQ=255)
                                        tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                    <  2:607123/141‑10 (MQ=255)
                                        tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                    <  1:403497/141‑15 (MQ=11)
                                        tatCGTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGCTATTATTTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                    >  1:723699/1‑127 (MQ=11)
                                         atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtac                                                                                                   >  2:229069/1‑131 (MQ=11)
                                         atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaa                                                                                                   >  2:661945/1‑131 (MQ=255)
                                         atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTAATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCACCTTTGAACCAGAACAGcattttataagtac                                                                                                   >  2:825977/1‑131 (MQ=11)
                                           cTTAGATAGTAGCCGACGGGTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtaatgtatgggtacac                                                                                                 >  1:583934/1‑127 (MQ=11)
                                            ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact                                                                                                >  1:851587/1‑128 (MQ=11)
                                            ttAGATAGTAGCCAACGGCTACAGTGACAACAGCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACCGCACCGTATTATGGAATAGAGAt                                                                                                <  2:48523/141‑1 (MQ=255)
                                             tAGATTGTATGCAACGGCTACAGTGATAACATCCCTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                               <  2:750601/141‑15 (MQ=11)
                                             tAGATAGTAGCCAACGGCTACAGTGATAACCGCCTGGTGAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttacgagtacactc                                                                                               <  2:219792/141‑15 (MQ=11)
                                             tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                               <  1:278204/141‑15 (MQ=11)
                                             tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAATTTTTTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                               <  1:205135/141‑15 (MQ=11)
                                               gATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGtt                                                                                             <  2:548503/141‑1 (MQ=255)
                                                  agtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGCCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGt                                                                                          >  2:377102/1‑141 (MQ=255)
                                                          aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTTTTATGGAATAGAGAGGTTGGTAACATTta                                                                                  <  1:566526/141‑1 (MQ=255)
                                                          aCGGCTACAGTGATAACATCCTTGTTAAACTGTTTATATCTGAAATAGTTCCTACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta                                                                                  <  2:562022/141‑1 (MQ=255)
                                                           cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                 <  2:663039/141‑1 (MQ=255)
                                                             gCTACAGTTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATAc                                                                               <  1:268731/141‑1 (MQ=255)
                                                                aCAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAgg                                                                            >  1:174229/1‑141 (MQ=255)
                                                                 cAGTTATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTCGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGa                                                                           <  2:475285/141‑1 (MQ=255)
                                                                               ttGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAg                                                             >  1:39155/1‑141 (MQ=255)
                                                                               ttGTTAAATTGTTTATAACTGAAATAGTTCATACAGAAGACGCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAg                                                             <  2:433065/141‑1 (MQ=255)
                                                                                tGTCAAATTGTTTAGAGCCGAAATAGTTCATACAGAGGACTCATTTTTGTTAAAATTATACTATACATTCAACTTTGAAACAGAACAGTATTACGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGt                                                            <  1:598639/141‑1 (MQ=21)
                                                                                 gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGCCTCATTTTCGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTAGGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                           <  2:1015455/141‑1 (MQ=255)
                                                                                 gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                           >  1:910189/1‑141 (MQ=255)
                                                                                    aaaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTaa                                                        >  2:421460/1‑141 (MQ=255)
                                                                                      aTTGGTTATATCTGAAATAGTTCAGACAGAAGACGCCTTTTTGTTAGAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGCAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                      <  1:364896/141‑1 (MQ=25)
                                                                                           ttATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtt                                                 >  1:944366/1‑141 (MQ=255)
                                                                                           ttATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAACGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtt                                                 >  2:553473/1‑141 (MQ=255)
                                                                                            tataTCGGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                                <  1:966716/141‑1 (MQ=255)
                                                                                             atatCTGAAATAGTTAATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttat                                               >  1:790940/1‑141 (MQ=255)
                                                                                                 cTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACa                                           <  1:742127/141‑1 (MQ=255)
                                                                                                 cTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACa                                           >  1:829102/1‑141 (MQ=255)
                                                                                                           tCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacaca                                 >  1:82678/1‑141 (MQ=255)
                                                                                                           tCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACATAAGTTTAATTTCGTTATTACAGAACcacaca                                 >  1:709495/1‑141 (MQ=255)
                                                                                                           tCATACACAACACTCCCTATTTTTGAAATTATACTATAAATTCAACATTGCAAACCAACCCACGTATTATATAGAGATATCGGTATACTTTATAAAGGATCATTATAATTAAGTTTAATTTCGGTACTACAGAACcacacc                                 >  2:270669/1‑140 (MQ=255)
                                                                                                              tACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTc                              <  2:1031138/141‑1 (MQ=255)
                                                                                                               aCAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATCATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTcc                             <  2:761227/141‑1 (MQ=255)
                                                                                                                cAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAAGTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTACGTTTAATTTCGTTATTACAGAACCACACATTCCa                            >  2:237828/1‑141 (MQ=255)
                                                                                                                   aagaCTCCTTTTTGTTAAAATTATACTGTAAATTCAACTTTGCACCAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACGGAACCACACATACCAAcc                         >  1:725355/1‑141 (MQ=255)
                                                                                                                   aagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAAcc                         >  2:243952/1‑141 (MQ=255)
                                                                                                                   aagaCTCCTTTTTGATAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTAAATAACCACACATTACAccc                         >  2:719469/1‑141 (MQ=38)
                                                                                                                     gaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGTTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCag                       >  2:408174/1‑141 (MQ=255)
                                                                                                                     gaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCag                       <  1:860584/141‑1 (MQ=255)
                                                                                                                     gaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCag                       <  2:414258/141‑1 (MQ=255)
                                                                                                                        tCCTTTTTGTTATAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaag                    >  2:650625/1‑141 (MQ=255)
                                                                                                                              ttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGCATAGAGATGTTGGTAACATTTATACAGGATCATTATACGTAAGTGTAAGTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAg              >  2:223041/1‑141 (MQ=255)
                                                                                                                              ttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGACGAGAAAg              >  1:235967/1‑141 (MQ=255)
                                                                                                                              ttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAg              >  1:623974/1‑141 (MQ=255)
                                                                                                                                 ttAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCATTATTCCAGAAACACACATNCCATACAGAAGAGAAAGTAt           >  1:616598/1‑141 (MQ=39)
                                                                                                                                   aaaaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGCTCATTATACTTAAGTTTAATTTCGTTATTACAGGACCACACATTCCAACAAGAAGAGAAAGtcggt         >  2:417686/1‑137 (MQ=255)
                                                                                                                                          taCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACCCATTCCAACCAGAAGAGAAAGTATGTCTATTTa  >  1:527837/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
TTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTA  >  CP000730/36441‑36719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: