Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,817 A→T 36.5% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8170AT36.5% 208.8 / 135.6 129intergenic (+48/+60)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (48/33);  new base T (22/25);  total (70/59)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

Only 100 of 133 total aligned reads displayed.

GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35677‑35956
                                                                                                                                            |                                                                                                                                           
gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                             <  1:226429/141‑1 (MQ=255)
gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTCAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                             >  1:401120/1‑141 (MQ=255)
   aaTTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGt                                                                                                                                          <  1:214245/141‑1 (MQ=255)
     ttattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTc                                                                                                                                        >  1:1035072/1‑141 (MQ=255)
     ttattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTc                                                                                                                                        >  1:618682/1‑141 (MQ=255)
     ttattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATGGTGATCTAATAAAAATAGAAGGTACt                                                                                                                                       >  2:676882/1‑141 (MQ=255)
       attCTGAATTTAAAGAGGTAGATTATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTg                                                                                                                                      <  2:283444/141‑1 (MQ=255)
           tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTGCTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATCGAAGGTTCTGTTGc                                                                                                                                  >  2:615430/1‑141 (MQ=255)
           tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                  >  1:847938/1‑141 (MQ=255)
            gAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTATTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAGTTGTGATCTAATAAAAATAGAAGGTTATGTTGCa                                                                                                                                 >  2:818614/1‑141 (MQ=255)
            gAATTTAAAGATGATGATGATCTTATTGACAAAGTAGTTAATTTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCa                                                                                                                                 <  2:414856/141‑1 (MQ=255)
             aaTTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCaa                                                                                                                                <  1:176152/141‑1 (MQ=255)
              aTTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCaaa                                                                                                                               <  1:998257/141‑1 (MQ=255)
               tttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAGGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAg                                                                                                                              <  1:32713/141‑1 (MQ=255)
                   aaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaa                                                                                                                          <  1:326681/141‑1 (MQ=255)
                   aaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAAAAAAAATAGAAGGTGCTGTTGCAAAGTaaa                                                                                                                          >  2:102741/1‑141 (MQ=255)
                   aaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCGAAAATAAAATTGTGATTTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaa                                                                                                                          <  1:703089/141‑1 (MQ=255)
                        gTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                     <  1:860156/141‑1 (MQ=255)
                                 ctcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAc                                                                                                            <  1:341514/141‑1 (MQ=255)
                                                tgtAATGGTGAAGCTATTGAGTATAAAGGTTTTGGCGAAAAATGATTACTTGAACAAGGGATAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGGAAAGGAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                             <  2:532993/139‑1 (MQ=37)
                                                   aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGAGTACTGGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                          >  1:560485/1‑141 (MQ=255)
                                                     tatAGAAGCTATTCAGTATAAAGATTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATAGTAATACACAACAATCAACAGTTTCTGTTTCAAAGTCCAAAAATATAGCTAACCACTAATTTATCATGTCAgt                                                                                        <  2:224134/139‑1 (MQ=21)
                                                      gTAGAAGATATTCAGTATAAAGTTTTTGGCGAAAAATGGTTGCTTGAACAAGAGCTAAAATCAAATTGTGATCTAATAAAAATAGAAGGTGCTGTTGCAAAGTAAAAAAATATAGCTACCCACTAATTTATTATGTAAgtt                                                                                       >  2:425298/1‑140 (MQ=37)
                                                       tAGAAGCTATTCAGTATAAAGTTTTTTTTTTTTTATGATTACTTGAACAAGAGATAAAATAAAATTGTGACCTAATAAAAATAGAAGGTGCTGTTTCAAAGTAAAAAAATATAGCTAACCACTAATTTAGAATGTTAgttt                                                                                      >  2:954048/1‑139 (MQ=25)
                                                       tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                      <  1:38360/141‑1 (MQ=255)
                                                         gAAGCTATTCAGTATCAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTATGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTCAAAAAAAATAGCTAAACACTAATTTATCaatatgatgttc                                                                                    >  2:822882/1‑130 (MQ=255)
                                                         gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTc                                                                                    >  2:807098/1‑141 (MQ=255)
                                                         gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTc                                                                                    >  1:251378/1‑141 (MQ=255)
                                                         gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAACACATCATGCTTTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTc                                                                                    >  1:503491/1‑141 (MQ=37)
                                                             cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                <  1:728697/141‑1 (MQ=255)
                                                               aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTaa                                                                              <  1:575768/141‑1 (MQ=255)
                                                               aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCATaa                                                                              >  1:340785/1‑141 (MQ=255)
                                                                     tataAAGTTTTTGGTGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                        <  2:701441/141‑1 (MQ=255)
                                                                     tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTCCt                                                                        >  2:864405/1‑141 (MQ=255)
                                                                     gataAAGGTTATGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                        <  1:981403/140‑1 (MQ=255)
                                                                       taAAGTTTTTGGCGAAAAAAGATTACTTGAACAAGAGCGAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAg                                                                      >  1:592934/1‑141 (MQ=255)
                                                                         aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                    >  1:535387/1‑141 (MQ=255)
                                                                         aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                    >  2:302298/1‑141 (MQ=255)
                                                                            tttttGGCGAAAAATGATTACTTGAACAAGAGCTCAAACAAAAGTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatga                                                                 >  1:1048009/1‑141 (MQ=255)
                                                                              tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATCGCTAACCACTAATTTATCATGTCAGTGCTCGCTTAACTTGCTAGCatgatg                                                               <  2:418202/141‑1 (MQ=255)
                                                                                tGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCt                                                             >  1:613353/1‑141 (MQ=255)
                                                                                 ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTTGCTTAACTTGCTAGCATGATGCTa                                                            >  1:23376/1‑141 (MQ=255)
                                                                                 ggCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                            >  1:784829/1‑141 (MQ=255)
                                                                                             ttACTTGCACAAGAGCTAAATTAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGAAAAAAAATATAGCTAACCTCTAATTTATCATGTCAGTGTTCGATTAACTTGCTTGCATGATGCTAATTTCGTGGCAt                                                >  1:669136/1‑141 (MQ=18)
                                                                                             ttACATGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCAt                                                >  2:733932/1‑141 (MQ=255)
                                                                                                          agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATGGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                   >  1:649288/1‑141 (MQ=255)
                                                                                                            cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTCACTTGCTAGCCTGATGCTAATTTCGTGGCATGGCGAAAGCCCGTAg                                 >  2:131953/1‑141 (MQ=255)
                                                                                                             taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCCCTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                >  1:18956/1‑141 (MQ=255)
                                                                                                                aataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCt                             >  1:630667/1‑141 (MQ=255)
                                                                                                                 acaaaagagtattttaataaAAATGATGGTTATGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                          >  2:886357/15‑141 (MQ=11)
                                                                                                                 acaaaagagtattttaataaAAATGATGGGTCTGTTGCAAAGTAATAAATATAGCTAATAACTAATATATCATGTCAGTATTCGTTTAAATTGCTAGCATGATGCTAATTTCATGGCATGGGGCAAATCCGCAGATCTGaa                          >  2:514646/15‑141 (MQ=2)
                                                                                                                   aactgagtattttaataaAAATGATGGTTCTGTTGCAAAGTAATAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  2:728483/129‑1 (MQ=11)
                                                                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  1:679751/129‑1 (MQ=11)
                                                                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  1:878693/129‑1 (MQ=11)
                                                                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  2:855521/129‑1 (MQ=11)
                                                                                                                    aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCATAGATCTggaa                         >  1:891641/1‑138 (MQ=255)
                                                                                                                     aatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTGATTTATCAGGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        >  2:908930/1‑141 (MQ=255)
                                                                                                                     aatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        >  2:1044580/1‑141 (MQ=255)
                                                                                                                     aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                      <  2:20577/131‑1 (MQ=11)
                                                                                                                      agattattCTAATAAAAATGACGCTTCTGTTGTAAAGTAAAAAATATAGCTAATCACCAATATATCCTGTCAGTGTGCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAgtgaa                     <  2:180925/134‑2 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTCATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:936461/132‑1 (MQ=11)
                                                                                                                        tgtgATCTAATAAAACTAGAAGGTTCTGTTGCAAAGTAAAGAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGGTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:929180/141‑1 (MQ=255)
                                                                                                                        tgtgATCTAATAAAAATAGAACGTTCTACTCAAACGAACAAAAATATAGCCAACCGCTGATTTGTCAGGTCAATTTTCGCTGAACTGTGTACCATTATGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:354817/141‑1 (MQ=9)
                                                                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTCATCACTAATATCTCATGTCATTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                   <  2:377173/134‑1 (MQ=11)
                                                                                                                         gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    >  2:1055877/1‑141 (MQ=255)
                                                                                                                         gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:946563/141‑1 (MQ=255)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATATCTAATTACTAATATTTTTTGTTTGTTTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 >  2:706637/6‑141 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:1049656/136‑1 (MQ=11)
                                                                                                                           gagcTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCCGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  2:339580/138‑1 (MQ=255)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  1:317039/137‑1 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                >  1:752790/5‑141 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  1:809754/137‑1 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                >  2:842339/5‑141 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                >  1:415276/5‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGTTGGTTCTCTTGCAAAGTAAAAAATTTAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:93710/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTGATATATCACGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:40379/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               >  1:24355/4‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTCAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               >  2:1037636/4‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATAGAACTGATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               >  1:250794/4‑141 (MQ=11)
                                                                                                                            aTCTAATAAAAATCGAAGGTTCTTGTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:286100/141‑1 (MQ=255)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGTAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  1:128496/139‑1 (MQ=11)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  2:807554/139‑1 (MQ=11)
                                                                                                                              ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATTGCTAATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTCGCATGATGCTAATTTCGTGGCATGGCGAAGATCTGTAGCTCTGTAGGGACCTGCGGtt             >  1:63753/2‑141 (MQ=11)
                                                                                                                              ttaataaAAATGATGGTACTGTTGCAAAGTAAAAAATATAGCTAATCATTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  2:618766/140‑1 (MQ=11)
                                                                                                                              tcatattaaaTAGCTGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCCGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGGGAAAATCCGTAGATCTGAAGAGAACTACGGt              >  2:326890/8‑141 (MQ=11)
                                                                                                                              taaaaaaataTAAAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:634667/136‑1 (MQ=12)
                                                                                                                              cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGAgg               >  1:339667/1‑141 (MQ=255)
                                                                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGGTCTGAAGAGACCTGCGGTTc            >  2:296800/1‑141 (MQ=11)
                                                                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc            <  1:362143/141‑1 (MQ=11)
                                                                                                                               taataaAAATGATGGATCTGTTGCAAAGTAAAAATGATAGCTCATCACTAATATATCATGTCAGCGTACGTTGAGCTTGCTAGCATGATGATAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc            >  1:792935/1‑141 (MQ=11)
                                                                                                                                aataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGAATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             >  2:405487/1‑141 (MQ=255)
                                                                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           >  1:43771/1‑141 (MQ=11)
                                                                                                                                  taaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           <  1:411365/141‑1 (MQ=38)
                                                                                                                                  taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt         <  1:488396/141‑1 (MQ=11)
                                                                                                                                     aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt        >  2:496871/1‑141 (MQ=35)
                                                                                                                                        tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat     <  1:98650/141‑1 (MQ=32)
                                                                                                                                         aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata    <  2:75944/141‑1 (MQ=32)
                                                                                                                                          gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat   >  2:213725/1‑141 (MQ=31)
                                                                                                                                           aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGGATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata  <  1:727516/141‑1 (MQ=11)
                                                                                                                                           aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata  >  1:650425/1‑141 (MQ=11)
                                                                                                                                            |                                                                                                                                           
GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35677‑35956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: