Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 215,097 T→C 5.2% K164E (AAA→GAA)  argC ← N‑acetyl‑gamma‑glutamyl‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730215,0970TC 5.2% 223.6 / 3.0 79K164E (AAA→GAA) argCN‑acetyl‑gamma‑glutamyl‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (29/42);  new base C (2/2);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

TGTGTTTATGGTTTCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCAAATCATCTTGTG  >  CP000730/214959‑215234
                                                                                                                                           |                                                                                                                                         
tgtgTTTATGGTTTCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTAtttttt                                                                                                                                         >  2:806300/1‑141 (MQ=255)
  tgtTTATTGTTTCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGCGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCg                                                                                                                                       <  2:21730/141‑1 (MQ=255)
  tgtTTATGGTTTCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCCCCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCg                                                                                                                                       >  1:294698/1‑141 (MQ=255)
  tgtTTATGGTTTCCGATTGCATAAGAGCTTTGATTTTCATACATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAACAATAGACCACAAA‑TTTACTCTTTGTTCg                                                                                                                                       <  2:219860/141‑1 (MQ=255)
  tgtTTATGGTATGCCCTTGCATAATCGCTTAGATTTTCATTCATTTCTGCAAAATGAACACGTTGTGATAATGATCGACCAGCACCTGACCCGCCGGTCGTAGCATCTATAATAATAG‑CGACAAACTCAACTAtttttgcc                                                                                                                                       >  2:491919/1‑138 (MQ=255)
      tATGGTTTACGATTCCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCtaa                                                                                                                                   <  1:907821/141‑1 (MQ=255)
       aTGGTTTCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCtaat                                                                                                                                  <  1:310698/141‑1 (MQ=255)
             tCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTgg                                                                                                                            <  2:666960/141‑1 (MQ=255)
             tCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTgg                                                                                                                            <  1:399437/141‑1 (MQ=255)
              ccGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGa                                                                                                                           <  2:202378/141‑1 (MQ=255)
              ccGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGa                                                                                                                           <  1:306525/141‑1 (MQ=255)
                caTTGCATAAGCGCTTAGTTTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGACGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATg                                                                                                                         <  1:974692/140‑1 (MQ=255)
                    gCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGCCAAA‑TCTACTATTTTTTCGCTAAAAAGTGGATGTAAt                                                                                                                     >  2:300281/1‑141 (MQ=255)
                    gCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTACTAAGTGGATGTAAt                                                                                                                     >  1:458181/1‑141 (MQ=255)
                     cATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATg                                                                                                                    <  1:1012567/141‑1 (MQ=255)
                      aTAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGc                                                                                                                   <  2:674350/141‑1 (MQ=255)
                      aTAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGc                                                                                                                   <  2:245815/141‑1 (MQ=255)
                      aTAAGCGCGTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGc                                                                                                                   <  2:245844/141‑1 (MQ=255)
                        aaGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCta                                                                                                                 <  1:1002883/141‑1 (MQ=255)
                        aaGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCta                                                                                                                 <  1:1028686/141‑1 (MQ=255)
                           cgcTTAGATTTTCATCCATTTCTGAAAAATGAACCCGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCtaata                                                                                                              <  2:895223/141‑1 (MQ=255)
                            gcTTAGATTTTCCTTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCtaataa                                                                                                             <  2:869334/141‑1 (MQ=255)
                                   ttttCATTCTTTTATGAAAAATTAACACGTAGTGCTAATGAACCGCGCGGTCCGGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCt                                                                                                      <  1:551262/141‑1 (MQ=255)
                                     ttcattcaTTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTCATAATATTGCTGt                                                                                                    >  2:630136/1‑141 (MQ=255)
                                      tcattcatTTCTGAAAGATGACCACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTa                                                                                                   >  1:544624/1‑141 (MQ=255)
                                       cattcattTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAg                                                                                                  >  2:929979/1‑141 (MQ=255)
                                       cattcattTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTCTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAg                                                                                                  >  2:919557/1‑141 (MQ=255)
                                       cattcattTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTCTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAg                                                                                                  >  2:919567/1‑141 (MQ=255)
                                         ttcattTCTTAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCTGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAggg                                                                                                <  2:617366/141‑1 (MQ=255)
                                         ttcattTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGTTATTATTTTGATGTAATGCTAATAATGTTTCTGTAggg                                                                                                >  2:17665/1‑141 (MQ=255)
                                         ttcattTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAggg                                                                                                <  2:799737/141‑1 (MQ=255)
                                           cattTCTGAAAAATGAACACGTTGTGCTAAAGAACGACCAACACCTGACACGCCGGTCTTAGCATCCATAAGAATAGATAACAAA‑ACTACGATATTTTCGCTAATAAGTGGATGTAATGCTAAAAATGTGGCGGTAGGGaa                                                                                              >  1:483412/1‑141 (MQ=255)
                                             tttCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAAc                                                                                            >  2:516587/1‑141 (MQ=255)
                                                cTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGc                                                                                         <  2:273380/141‑1 (MQ=255)
                                                 tGAAAAATGAACACGTGGTGATAATGCACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGcc                                                                                        >  2:179955/1‑141 (MQ=255)
                                                    aaaaTGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTCTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAgg                                                                                     <  1:919567/141‑1 (MQ=255)
                                                    aaaaTGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTCTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAgg                                                                                     <  1:919557/141‑1 (MQ=255)
                                                     aaaTGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCCGGa                                                                                    >  2:171708/1‑141 (MQ=255)
                                                       aTGAACCCGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGAtt                                                                                  <  1:1010007/141‑1 (MQ=255)
                                                       aTGAACACGTCGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGAtt                                                                                  <  1:185778/141‑1 (MQ=255)
                                                          aaCACGTTGTGATAATGAACGAACAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGa                                                                               <  2:346537/141‑1 (MQ=255)
                                                              cGTTGTGAGAATGAACGACCAGTACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTGTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATg                                                                           <  1:159646/141‑1 (MQ=255)
                                                               gtggtgATAATGAGCGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGa                                                                          <  2:765350/138‑1 (MQ=255)
                                                               gTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGa                                                                          <  2:974422/141‑1 (MQ=255)
                                                               cttGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGa                                                                          <  2:940994/140‑1 (MQ=255)
                                                                        aTGAACGACCAGCACCTGACACGCCGGTCTCAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTAGAGGGAAAGAGAAAAGATTAGAAATGAGCTTCGTTc                                                                 >  2:939422/1‑141 (MQ=255)
                                                                         agAACGACCAGCACCTTACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTcc                                                                <  2:979190/140‑1 (MQ=255)
                                                                         agAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTcc                                                                <  2:627774/140‑1 (MQ=255)
                                                                          taaCGACCAGCACCTTACACGCAGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCa                                                               <  2:879941/140‑1 (MQ=255)
                                                                          gAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTATAGGGAAACAGCCAGGATTAGAAATGGACTGCGTTcct                                                               >  2:13868/1‑140 (MQ=255)
                                                                              gACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGt                                                           >  1:360174/1‑141 (MQ=255)
                                                                               aCCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGtt                                                          >  1:367912/1‑141 (MQ=255)
                                                                               aCCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGtt                                                          >  1:635093/1‑141 (MQ=255)
                                                                                 cAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTAt                                                        <  2:676952/141‑1 (MQ=255)
                                                                                  aGCACCTGACACGCCGGTCTTAGCATCAGTAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCTTTGTTATc                                                       >  1:40161/1‑141 (MQ=255)
                                                                                  aGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTCGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATc                                                       <  1:580095/141‑1 (MQ=255)
                                                                                    cACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCaa                                                     >  1:9959/1‑141 (MQ=255)
                                                                                     aCCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTTCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCaaa                                                    <  2:170838/141‑1 (MQ=255)
                                                                                      ccTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAAAAGCCAGGATTAGAAATGCGCGTCGTTCCATTGTTATCaaag                                                   >  1:1039331/1‑140 (MQ=255)
                                                                                         gACCCGCCGTGCTTAGAATCAATAATAATCGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGTGAAATTGAACGAATCAAAAATGAGCTTCGTTCCATTGTTATCAAACGAt                                                <  1:1047787/141‑1 (MQ=255)
                                                                                          acacGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATGGTTATCAAACGAtt                                               >  2:821280/1‑141 (MQ=255)
                                                                                          acacGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGATTCGTTCAATTGTTATCAAACGAtt                                               >  1:870946/1‑141 (MQ=255)
                                                                                             cGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGcc                                            >  2:50184/1‑141 (MQ=255)
                                                                                                cGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCAtt                                         <  2:977349/141‑1 (MQ=255)
                                                                                                cGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCAtt                                         >  1:920928/1‑141 (MQ=255)
                                                                                                  gTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCGGGATAAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCt                                       >  2:744681/1‑141 (MQ=255)
                                                                                                  gTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATTAAACGATTGCCATTCt                                       <  1:159147/141‑1 (MQ=255)
                                                                                                   tCTTAGCATCAAGAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAACGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTg                                      <  1:593964/141‑1 (MQ=255)
                                                                                                       aGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGGGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAAt                                  >  1:1045588/1‑141 (MQ=255)
                                                                                                          aTCAATAATAATAGATGACAAA‑TCTACTATTTGTTCGCTATTAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGACATTCTGAAATGCt                               >  1:759262/1‑141 (MQ=255)
                                                                                                                aataataGATGACAAA‑TCTACTATTTTTTCTCTTATAAGTGGATGTAATGCTAATAATGTTGCAGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACAATTCCCATTCTCAAATACTGTAAAt                         >  2:366233/1‑141 (MQ=255)
                                                                                                                aataataGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAAt                         >  1:372141/1‑141 (MQ=255)
                                                                                                                 ataataGATGACAAA‑TCTACTATTTTTTCTCTTATATTTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTTAAATGCTGTAAAtt                        >  2:8821/1‑140 (MQ=255)
                                                                                                                   aataGATGACAAA‑TCTACTATTTGTTCGCTAATAAGTGGATGTAATGCTAACAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGc                      <  1:822625/141‑1 (MQ=255)
                                                                                                                    ataGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTATAAATTATCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCa                     <  2:584099/141‑1 (MQ=255)
                                                                                                                     taGATGACAAA‑TCTACTATTTTTTCGCTAATAAGGGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCAt                    <  1:229612/141‑1 (MQ=255)
                                                                                                                         tGACAAA‑TCTACCTTTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGAtt                <  2:1006432/141‑1 (MQ=255)
                                                                                                                           aCAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCa              <  1:806300/141‑1 (MQ=255)
                                                                                                                                   tactaTTTTTTCGCTAAGAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCAAatcatc       <  2:899191/141‑1 (MQ=255)
                                                                                                                                   tactaTTCTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAATCCGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCAAatcatc       <  1:876710/141‑1 (MQ=255)
                                                                                                                                     ctaTTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCAAATCATCtt     >  1:162768/1‑141 (MQ=255)
                                                                                                                                        ttttttCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCAAATCATCTtgtg  <  1:418982/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TGTGTTTATGGTTTCCGATTGCATAAGCGCTTAGATTTTCATTCATTTCTGAAAAATGAACACGTTGTGATAATGAACGACCAGCACCTGACACGCCGGTCTTAGCATCAATAATAATAGATGACAAA‑TCTACTATTTTTTCGCTAATAAGTGGATGTAATGCTAATAATGTTGCTGTAGGGAAACAGCCAGGATTAGAAATGAGCTTCGTTCCATTGTTATCAAACGATTGCCATTCTGAAATGCTGTAAATAGCATGATTCAAATCATCTTGTG  >  CP000730/214959‑215234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑