BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,231,474	G→A	4.4%	G40D (GGT→GAT)	USA300HOU_1156 →	possible PP2C protein phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,231,474	0	G	A	4.4%	305.4 / 2.2	103	G40D (GGT→GAT)	USA300HOU_1156	possible PP2C protein phosphatase
Reads supporting (aligned to +/- strand): ref base G (41/57); new base A (2/3); total (43/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 106 total aligned reads displayed.

AGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATAAATTTTCAGT  >  CP000730/1231334‑1231611

aGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATggg                                                                                                                                           <  1:545143/141‑1 (MQ=255)

aGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACAAATCAACAACTTTTAGTTCTGTGTGATGGTATtgg                                                                                                                                           >  2:885567/1‑141 (MQ=255)

aGTAGAAACGAAGGAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGTGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATggg                                                                                                                                           <  1:187679/141‑1 (MQ=255)

     aaaCGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGcc                                                                                                                                      >  1:337966/1‑141 (MQ=255)

          aGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGAGAAGATAGGGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTATGTGTGATGGTATGGGTGGCCATaaa                                                                                                                                 >  1:465920/1‑141 (MQ=255)

              aaaGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAg                                                                                                                             <  2:518172/141‑1 (MQ=255)

              aaaGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAg                                                                                                                             <  2:980290/141‑1 (MQ=255)

              aaaGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAg                                                                                                                             >  1:654704/1‑141 (MQ=255)

               aaGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGACTGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGCTGGTGTGGGTGGCCATAAAGCggg                                                                                                                            >  1:928938/1‑141 (MQ=255)

               aaGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAgg                                                                                                                            <  1:185794/141‑1 (MQ=255)

                aGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTACAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGga                                                                                                                           >  2:761060/1‑141 (MQ=255)

                  aCAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGCTGGTATGGGTGGCCATCAAGCAGgaga                                                                                                                         >  1:50855/1‑141 (MQ=255)

                  aCAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGgaga                                                                                                                         >  1:50772/1‑141 (MQ=255)

                   cAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGGCATAAAGAAGgagga                                                                                                                        >  2:1032201/1‑139 (MQ=255)

                    aaaTGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAAC‑TTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGt                                                                                                                      <  1:536999/141‑1 (MQ=255)

                     aaTGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGt                                                                                                                      <  1:82477/141‑1 (MQ=255)

                       tGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTg                                                                                                                    >  1:186584/1‑141 (MQ=255)

                        gCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGTTGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGc                                                                                                                   <  1:59174/141‑1 (MQ=255)

                               gCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTaaa                                                                                                            <  2:480866/141‑1 (MQ=255)

                                 acaATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAAtt                                                                                                          >  2:695979/1‑141 (MQ=255)

                                  caATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAAttt                                                                                                         <  2:463197/141‑1 (MQ=255)

                                    aTTTTTTACTGATACTGGAAAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGt                                                                                                       <  1:185368/141‑1 (MQ=255)

                                      tttttACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTa                                                                                                     <  2:510845/141‑1 (MQ=255)

                                                tACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGtt                                                                                           >  1:97031/1‑141 (MQ=255)

                                                  atGGACCATATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTGAGAATTAAACTAAACAAGAACTTTTAGTTCTGTGTGGTGGTAAGAATGGTTAAATGGGAGGTTAAGTGGTATGTATATTTGTTACAGATGAGTTGa                                                                                         <  2:486626/140‑1 (MQ=255)

                                                   tGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGaa                                                                                        <  2:186584/141‑1 (MQ=255)

                                                       caacaTAGAGATAAGAATGAAGAAAAGTATGGTT‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATcc                                                                                    >  1:513988/1‑141 (MQ=255)

                                                        aacaTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATgcc                                                                                   >  1:311756/1‑141 (MQ=255)

                                                         acaTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGTAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAGATCCCg                                                                                  <  2:666427/141‑1 (MQ=255)

                                                         acaTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGTAGAAGTTGCAAGGAAATTTGTTACAGGTGAGTTGAAATCCCg                                                                                  <  1:897341/141‑1 (MQ=255)

                                                          caTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGt                                                                                 >  1:589988/1‑141 (MQ=255)

                                                          caTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGt                                                                                 <  1:194028/141‑1 (MQ=255)

                                                          caTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGt                                                                                 >  1:487343/1‑141 (MQ=255)

                                                           aTAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGtt                                                                                >  1:490294/1‑141 (MQ=255)

                                                            tAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGttt                                                                               <  1:547265/141‑1 (MQ=255)

                                                            tAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGttt                                                                               >  2:685198/1‑141 (MQ=255)

                                                              gagaTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGACAAAGATTCCTGAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTg                                                                             >  1:771774/1‑141 (MQ=255)

                                                               agaTAAGACTGAAGATGCGGGGGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGa                                                                            <  2:983472/141‑1 (MQ=255)

                                                               agaTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGa                                                                            <  2:1040380/141‑1 (MQ=255)

                                                               agaTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGa                                                                            >  2:217616/1‑141 (MQ=255)

                                                                 aTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAg                                                                          <  1:135418/141‑1 (MQ=255)

                                                                 aTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAg                                                                          <  1:638679/141‑1 (MQ=255)

                                                                 aTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAATTGCAATTAAATTTGTTACAGATAAGTTGAAATCCCGTTTTGAAg                                                                          >  1:178318/1‑141 (MQ=255)

                                                                 aTAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGATGGCCATAAAGCAGGAGAAGCTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAg                                                                          <  1:837235/141‑1 (MQ=255)

                                                                   aaGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGCGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGct                                                                        <  1:300178/141‑2 (MQ=255)

                                                                    aGAATGAAGATGCGGGGGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCgg                                                                       <  2:552206/141‑1 (MQ=255)

                                                                      aaTGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGATGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGaa                                                                     >  2:249427/1‑141 (MQ=255)

                                                                         caaGATACAGCTGGCA‑TTCTTTAAAATCAAACTAATCAACAACGTTTAGTTCTGTTTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAAt                                                                  <  2:465920/140‑1 (MQ=255)

                                                                           aGATGCGGGTGGTA‑TTTTTTTTTTTTTTTTTAATCAACAACTGTTAGTTCTGTGTGATGGTATGGGTGGCCGTAAAGCAGGAGAAGTTGCCAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAATCGGAAAATCt                                                                >  2:1042464/1‑141 (MQ=255)

                                                                             aTGCGGGTGGGA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTta                                                              >  2:717599/1‑141 (MQ=255)

                                                                                cGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAg                                                           <  1:739655/141‑1 (MQ=255)

                                                                                 gggTGGTATTTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGAGGAAAATCTTATAg                                                           >  1:594059/1‑141 (MQ=255)

                                                                                 gggTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGa                                                          >  2:819392/1‑141 (MQ=255)

                                                                                   gtggtA‑TTTTTTATAATCAAAGTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGGTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGaac                                                        <  2:891536/141‑1 (MQ=255)

                                                                                   gtggtA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGaac                                                        <  2:870314/141‑1 (MQ=255)

                                                                                     ggtA‑TTTTTTATAATTAAACTAATCAGCAACTTTGAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAATTAAATCTGTTACATATGAGTTGAAATCCCGTTTTGAATCGGAAAATCTTATAGaacaa                                                      <  1:646391/141‑1 (MQ=255)

                                                                                     ggtA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGATTTGAAGCGGAAAATCTTATAGaacaa                                                      >  1:309435/1‑141 (MQ=255)

                                                                                          ttttttATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATc                                                  >  1:739070/1‑141 (MQ=255)

                                                                                                    gctaaCTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGaaaa                                        <  1:762158/138‑1 (MQ=255)

                                                                                                     cAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAAt                                       <  1:449698/141‑1 (MQ=255)

                                                                                                     cAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAAt                                       <  1:922908/141‑1 (MQ=255)

                                                                                                     cAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAAt                                       <  1:439697/141‑1 (MQ=255)

                                                                                                       aaCTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTg                                     <  1:809778/141‑1 (MQ=255)

                                                                                                        aCTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTgg                                    <  1:374248/141‑1 (MQ=255)

                                                                                                         cTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGt                                   <  1:1011461/141‑1 (MQ=255)

                                                                                                         cTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGt                                   >  1:161136/1‑141 (MQ=255)

                                                                                                           aaTCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACATCATCTAGCTGAAAATTGGTTg                                 <  1:546109/141‑1 (MQ=255)

                                                                                                           aaTCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTg                                 <  2:50772/141‑1 (MQ=255)

                                                                                                           aaTCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTg                                 <  1:685198/141‑1 (MQ=255)

                                                                                                           aaTCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGAAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTg                                 <  2:50855/141‑1 (MQ=255)

                                                                                                            aTCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGc                                >  2:1048604/1‑141 (MQ=255)

                                                                                                               aacaacTTTTAGTTCTGTGTGATGGTATGGATGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAATCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGta                             >  1:873170/1‑141 (MQ=255)

                                                                                                                acaacTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGTTGGAAATTGGTTGCGtaa                            >  2:151313/1‑141 (MQ=255)

                                                                                                                acaacTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGtaa                            <  1:110764/141‑1 (MQ=255)

                                                                                                                acaacTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGtaa                            >  2:151444/1‑141 (MQ=255)

                                                                                                                acaacTTTTAGTTCTGTGTGATGATATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTCAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAGGCTGAAAATTGGTTGCGtaa                            <  1:669496/141‑1 (MQ=255)

                                                                                                                 caacTTTTAGTTCTGTGTGATGGTCAGGGTGTCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAGACTGAAAATTGGGTGCGtaat                           >  2:1023111/1‑141 (MQ=255)

                                                                                                                 caacTATTACTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGTAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGtaat                           <  2:113728/141‑1 (MQ=255)

                                                                                                                 aaaattttAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGtaat                           <  2:51539/137‑1 (MQ=255)

                                                                                                                   aattgtAGTTTTATGTAAAGGTATGGGTGGCCATAAAGCAGGAGCATTTGCAAGTAAATTTGTTAAAGATGAGTTGAAATCCCGTTTTGAAGCGGAGAATCTTATAAAACTACATCAAGCTGAAAATTGGTTGCGcaattc                         <  2:11579/136‑3 (MQ=255)

                                                                                                                     ttttAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAataata                       <  2:847192/141‑1 (MQ=255)

                                                                                                                     ttttAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGGAataata                       >  1:94973/1‑141 (MQ=255)

                                                                                                                     ttttAGTTCTGTGTGATGGTATGGATGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGGGTTGAAATCCCGTTTGCAATCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAataata                       <  1:249427/141‑1 (MQ=255)

                                                                                                                       ttAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAAtata                     <  1:695979/141‑1 (MQ=255)

                                                                                                                        tAGTTCAGTGTGATGGCATGGGTGGCCATAAAGCAGGCGAAGTTGCCAGTAAATTTGTTACAGCTGAGTTGAAATCCCGTTTTGAAGCGGAAAGTCTTATAGAACAACATCAAGCTTCAAATTGGTTGCGCAATAATATaa                    >  2:128554/1‑141 (MQ=255)

                                                                                                                          gTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATaaaa                  >  1:878335/1‑141 (MQ=255)

                                                                                                                          gTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATaaaa                  >  1:12757/1‑141 (MQ=255)

                                                                                                                               gtgtgATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGatat             <  2:380041/141‑1 (MQ=255)

                                                                                                                               gtgtgATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGatat             <  2:879404/141‑1 (MQ=255)

                                                                                                                               gtgtgATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGatat             >  2:463525/1‑141 (MQ=255)

                                                                                                                               gtgtgATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGatat             <  1:885567/141‑1 (MQ=255)

                                                                                                                               gtgtgATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGatat             <  1:678986/141‑1 (MQ=255)

                                                                                                                               gtgtgATGGTAGGCGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTCTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGatat             <  1:141897/141‑1 (MQ=255)

                                                                                                                                 gtgATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTGCAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATaa           <  2:694347/141‑1 (MQ=255)

                                                                                                                                    gtGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATAAAtt        <  2:1040985/140‑1 (MQ=255)

                                                                                                                                    aTGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACTACATCAAGCTGTAAATTGGTTGCGTAATAATATAAAAGATATAAAtt        >  2:480199/1‑141 (MQ=255)

                                                                                                                                      ggTATGGGGGGCCATAAAGCAACAAAAGGTCCAAGTAAATTTGTTACAGATGAGTTGAAACCCCGTCTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAAGTGGTTGCGTAATAATATAAAAGATATGAAtttt      <  2:217024/141‑1 (MQ=255)

                                                                                                                                       gTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATAAATTTTc     <  1:28142/141‑1 (MQ=255)

                                                                                                                                       gTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGGAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATAAATTTTc     <  2:428242/141‑1 (MQ=255)

                                                                                                                                          tGGGTGGCCATAAAGCAGTGGAACTTTAAAGTAAATTTGTAACAGATGAGTTTAAATCCCGTTTTGAAGTGGAAAATCTTATAAAACAACATGAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATAAATTTTCAGt  <  2:928938/141‑1 (MQ=255)

AGTAGAAACGAGGTAAAGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTA‑TTTTTTATAATCAAACTAATCAACAACTTTTAGTTCTGTGTGATGGTATGGGTGGCCATAAAGCAGGAGAAGTTGCAAGTAAATTTGTTACAGATGAGTTGAAATCCCGTTTTGAAGCGGAAAATCTTATAGAACAACATCAAGCTGAAAATTGGTTGCGTAATAATATAAAAGATATAAATTTTCAGT  >  CP000730/1231334‑1231611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`