BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	27.0%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	27.0%	801.4 / inf	611	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (205/237); new base A (92/73); total (299/312)
Fisher's exact test for biased strand distribution p-value = 4.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 615 total aligned reads displayed.

GATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35800‑36080

attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGAAGAGACCTCCg                                                                                                                                            >  2:427998/5‑141 (MQ=11)

 ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTGATATATCACGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           <  2:40379/138‑1 (MQ=11)

 ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           <  1:810969/138‑1 (MQ=11)

 ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTCAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           >  2:1037636/4‑141 (MQ=11)

 ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATAGAACTGATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           >  1:250794/4‑141 (MQ=11)

   ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATTGCTAATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTCGCATGATGCTAATTTCGTGGCATGGCGAAGATCTGTAGCTCTGTAGGGACCTGCGGtt                                                                                                                                         >  1:63753/2‑141 (MQ=11)

   taaaaaaataTAAAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           <  2:634667/136‑1 (MQ=12)

   cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           >  1:653939/1‑141 (MQ=40)

              aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                                                                                <  2:75944/141‑1 (MQ=32)

               gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat                                                                                                                               >  2:213725/1‑141 (MQ=31)

               gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatat                                                                                                                               >  2:963771/1‑141 (MQ=31)

                                                aatcACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                                                                                              <  2:699055/138‑1 (MQ=0)

                                                          ttgtCATGTCAGTGGTCGCTTAACGTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAGAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTg                                                                                    <  2:321688/138‑1 (MQ=1)

                                                                   taGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  2:175157/140‑1 (MQ=11)

                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  1:951444/141‑1 (MQ=11)

                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         >  2:534153/1‑141 (MQ=12)

                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  2:598443/141‑1 (MQ=17)

                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAACTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  1:518436/141‑1 (MQ=17)

                                                                     gggtTTGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  1:228692/139‑1 (MQ=17)

                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                        >  1:323907/1‑141 (MQ=17)

                                                                      tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                        >  1:129961/1‑141 (MQ=32)

                                                                      tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                        >  1:942660/1‑141 (MQ=32)

                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                                                       <  1:799601/141‑1 (MQ=11)

                                                                       gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                       >  1:416993/1‑141 (MQ=17)

                                                                        ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  2:925738/141‑1 (MQ=17)

                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  2:618682/141‑1 (MQ=32)

                                                                         tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTGTTTATTTTGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTTATACTTTGAt                                                                     <  2:348416/141‑1 (MQ=11)

                                                                         tCGCTTAACTGTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                     <  1:463973/141‑1 (MQ=12)

                                                                          cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                    >  1:541108/4‑141 (MQ=17)

                                                                          cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACCTTGATa                                                                    >  2:249785/4‑141 (MQ=12)

                                                                             ttAACTTGCTATCACCATGCTAATTTCGTGTCATGGCGAAAATCCGTAGATCAGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  1:285571/141‑1 (MQ=11)

                                                                             ttAACTTGCTAGGATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  1:33614/141‑1 (MQ=17)

                                                                             ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCGGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTACAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 >  1:66975/1‑141 (MQ=12)

                                                                              tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                >  1:9239/1‑141 (MQ=17)

                                                                               aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                               <  2:865667/141‑1 (MQ=17)

                                                                                aCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGt                                                              <  2:199713/141‑1 (MQ=11)

                                                                                aCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCCTATTGATACTTTGATATCTTGt                                                              >  1:734471/1‑141 (MQ=11)

                                                                                    gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTTtcttt                                                          >  1:75663/1‑141 (MQ=12)

                                                                                    ccTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGCAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTTGATATCTTGtcttt                                                          <  1:745068/140‑1 (MQ=11)

                                                                                     cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                         <  2:676415/141‑1 (MQ=17)

                                                                                     cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACGCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttt                                                         >  1:594561/1‑140 (MQ=12)

                                                                                     cTAGCATGATACTACTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttt                                                         >  2:719707/1‑140 (MQ=11)

                                                                                      tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                        <  1:271476/141‑1 (MQ=17)

                                                                                        gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATAGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:1007847/1‑141 (MQ=17)

                                                                                        gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTCCGGTATTGCTACTTTGATACCTTGCCTTTCTTa                                                      >  2:741566/1‑141 (MQ=11)

                                                                                         catgattacaagttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAGACCTTGTCTTTCTTAc                                                     <  2:370710/129‑1 (MQ=11)

                                                                                         cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATATCTTGTCTTTCTTAc                                                     >  1:127415/1‑141 (MQ=11)

                                                                                         cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTCAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAGACTTTGATACCTTGTCTTTCTTAc                                                     >  1:881541/1‑141 (MQ=12)

                                                                                          atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATTCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    <  1:704755/141‑1 (MQ=21)

                                                                                          atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    >  1:301526/1‑141 (MQ=21)

                                                                                          atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    <  1:406628/141‑1 (MQ=21)

                                                                                          atgatgATAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACGTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    >  2:666763/1‑141 (MQ=255)

                                                                                           tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                   >  1:47370/1‑141 (MQ=255)

                                                                                           tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGCCTTGTCTTTCTTACtt                                                   <  1:841948/141‑1 (MQ=25)

                                                                                            gatgCTATTTTCGTGGCATGGCGAAAATTCGTAGATCTGAACTGAGCCGCGGTTTTTTTTATCGACAGCGTAAATACACTCAAGAGCTGTTAAAGTATTCTTTGCTGTGCTGATTCTTTGAGACCTTGTCTTTCATACttt                                                  <  2:266550/141‑1 (MQ=2)

                                                                                                cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                              >  2:390800/1‑141 (MQ=255)

                                                                                                 tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATGCTTTGATACCTTGTCTTTCTTACTTTAattt                                             >  1:578848/1‑139 (MQ=255)

                                                                                                 tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                             >  2:106371/1‑141 (MQ=21)

                                                                                                  aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                            <  1:972159/141‑1 (MQ=255)

                                                                                                  aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                            <  1:612440/141‑1 (MQ=25)

                                                                                                  aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGAGGTTCTTTTTTTTTATAGCGTAAATACTCTCAATACCTTTTAGGTTTTTCGGTGCTGTATGTATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                            <  2:1047565/141‑1 (MQ=11)

                                                                                                     ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                         >  1:495383/1‑141 (MQ=11)

                                                                                                     ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                         >  2:288700/1‑141 (MQ=25)

                                                                                                     ttCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCGGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTGATCTTTCTTACTTTAATATGACg                                         >  2:547753/1‑141 (MQ=11)

                                                                                                      tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTCAAGTTGCCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                        >  1:1040836/1‑141 (MQ=11)

                                                                                                      tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                        <  2:458987/141‑1 (MQ=37)

                                                                                                       cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                       <  2:136912/141‑1 (MQ=255)

                                                                                                       cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                       <  2:980696/141‑1 (MQ=255)

                                                                                                       cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                       >  1:1022064/1‑141 (MQ=37)

                                                                                                       cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAACATGACGGt                                       >  2:299882/1‑141 (MQ=255)

                                                                                                         tGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGATCGTAAATACATTCAATACCTTTTATAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGa                                     >  1:686458/1‑141 (MQ=11)

                                                                                                          ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATGCATTCAATACCTTTTTAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:72021/1‑141 (MQ=11)

                                                                                                          ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    <  2:503491/141‑1 (MQ=39)

                                                                                                          ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:55870/1‑141 (MQ=38)

                                                                                                           gCATGGGGAAAATCCGTAGGTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTCTGAAGTATTCTTTGCCGTATTGATTCTTTGATAACTTGTATTTCTTACTTTAACATGTCGGTCATc                                   >  1:389390/1‑141 (MQ=11)

                                                                                                           gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCTTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                   <  2:232068/141‑1 (MQ=11)

                                                                                                               tgagAAAATCCGTTGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  1:362587/138‑1 (MQ=255)

                                                                                                               ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGATGATAtttt                               >  2:522270/1‑139 (MQ=255)

                                                                                                                    aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:439361/1‑141 (MQ=38)

                                                                                                                    aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTACCGTATCCATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          <  1:201349/141‑1 (MQ=11)

                                                                                                                     aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         <  2:78161/141‑1 (MQ=255)

                                                                                                                     aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         >  2:1012570/1‑141 (MQ=255)

                                                                                                                        ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCATTGCgg                      >  2:445607/1‑141 (MQ=255)

                                                                                                                        ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      >  2:570842/1‑141 (MQ=37)

                                                                                                                        ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      >  2:152160/1‑141 (MQ=255)

                                                                                                                         cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  1:895481/141‑1 (MQ=255)

                                                                                                                           tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTCTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                   >  2:909126/1‑141 (MQ=255)

                                                                                                                            aggggtGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGATCAATGAGGTTAt                  >  2:651143/6‑141 (MQ=11)

                                                                                                                             gATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:839886/141‑1 (MQ=255)

                                                                                                                              aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATTCCTTATCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                >  1:990553/1‑141 (MQ=255)

                                                                                                                                cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:121405/141‑1 (MQ=255)

                                                                                                                                cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTCAGTTGTTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:770738/141‑1 (MQ=14)

                                                                                                                                cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  2:880915/141‑1 (MQ=255)

                                                                                                                                 ttgatagaCTTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:167424/136‑1 (MQ=14)

                                                                                                                                 tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             >  2:478530/1‑141 (MQ=255)

                                                                                                                                 tGAAGAGACCTGCGGGTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:863711/141‑1 (MQ=255)

                                                                                                                                         ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATGTTTCgt     >  2:470579/1‑140 (MQ=255)

                                                                                                                                         ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:216666/141‑1 (MQ=255)

                                                                                                                                           tGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAtt   >  1:62621/1‑140 (MQ=38)

                                                                                                                                           tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGGGATCTTGCTCAATGAGGTTATTCAGATATTTCGTTg   >  2:812106/1‑141 (MQ=255)

GATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35800‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`