BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,918	A→G	47.0%	intergenic (‑1001/‑167)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,918	0	A	G	47.0%	9.2 / 231.7	180	intergenic (‑1001/‑167)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (46/47); new base G (45/39); total (93/87)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01

Only 100 of 180 total aligned reads displayed.

GACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATT  >  CP000730/1929779‑1930056

gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                                                                           <  1:278438/141‑1 (MQ=255)

gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑CAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                                                                           <  1:714266/141‑1 (MQ=255)

   tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                                                                       <  2:185909/139‑1 (MQ=255)

       cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtgtt                                                                                                                                   >  1:322164/1‑141 (MQ=255)

        ttgttgatTTGCCGATGAGATAAGAGTAGAGTCTCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                                                                                   <  2:954764/135‑1 (MQ=255)

        ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                                                                                   <  2:16197/141‑1 (MQ=255)

        ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                                                                                   <  2:385319/141‑1 (MQ=255)

         tGTATATTTGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTTAATT‑AAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                                                                  <  1:933634/141‑1 (MQ=255)

          gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                                <  2:782078/130‑4 (MQ=255)

          gTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttt                                                                                                                                 <  1:157319/141‑1 (MQ=255)

            atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATAAGTGGCTGTGCTGTTGCGtattatact                                                                                                                              >  1:214158/10‑136 (MQ=255)

             tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                                                                             >  2:172044/9‑135 (MQ=255)

              attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTGATCAGTGGCTGTGCTGTTGCGTATTATACttt                                                                                                                            >  2:513247/8‑140 (MQ=255)

                 tGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGACGCCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTATTTCTCTTAtt                                                                                                                          >  2:175705/1‑141 (MQ=255)

                  caagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                        <  1:863092/138‑1 (MQ=255)

                  acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATTGAAGAGGCATGGACG‑GGCAT‑‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                        <  1:823026/138‑1 (MQ=255)

                  acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑‑TCGACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTGTACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                        <  2:994198/138‑1 (MQ=255)

                    agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGCGGAGCTTCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                      <  1:1040905/140‑1 (MQ=255)

                     gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                                                                                      >  1:542007/1‑141 (MQ=255)

                      atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                                                                     <  2:931290/141‑1 (MQ=255)

                      atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TACACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATTGCACCAGTCAGCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                                                                     <  2:399891/141‑1 (MQ=255)

                        ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                  <  1:655840/139‑1 (MQ=255)

                        ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                  >  1:1049589/3‑141 (MQ=255)

                        ggtataAAAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                  <  2:614834/139‑1 (MQ=255)

                         gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACTTAGTa                                                                                                                 >  1:59171/2‑141 (MQ=255)

                         gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                 >  2:18345/2‑141 (MQ=255)

                         gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                 <  2:539936/140‑1 (MQ=255)

                           ataataataGGGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                               <  2:838248/141‑3 (MQ=255)

                           ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                               >  2:144909/1‑139 (MQ=255)

                           ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                               <  2:911961/141‑3 (MQ=255)

                            aaataatAGAGCCGCCTATCGCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                              <  2:873598/140‑4 (MQ=255)

                              ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                            <  2:944018/141‑1 (MQ=255)

                                 atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                         <  1:955160/140‑2 (MQ=255)

                                  tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAGGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTCTTGGCTTAGTAAACGCaaca                                                                                                        >  2:566309/1‑139 (MQ=255)

                                   agagTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                       >  2:810943/1‑138 (MQ=255)

                                   aaagTCGCCTATCTCTCAGGCAGCAA‑‑‑TGGACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                       <  2:32700/139‑4 (MQ=255)

                                    gagTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                      <  1:203723/141‑5 (MQ=255)

                                    gagTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                      >  1:218811/1‑137 (MQ=255)

                                      gTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                                                                     >  1:858012/1‑141 (MQ=255)

                                      gTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                    >  1:40418/1‑135 (MQ=255)

                                      gTCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGGGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCTGTTGCGTATGATACTTATTGGCTTAGTAAAAGCaacaaata                                                                                                    >  2:970926/1‑135 (MQ=255)

                                       tCGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                                                                                   <  1:901024/141‑8 (MQ=255)

                                        cGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTCAGTAGACGCAATACaaaa                                                                                                   >  2:974809/1‑141 (MQ=255)

                                        cGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                                                                                   >  1:138422/1‑141 (MQ=255)

                                        cGCCTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGGAAACGCaacaaataat                                                                                                  >  2:344020/1‑133 (MQ=255)

                                         gCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                                                                  >  1:598935/1‑141 (MQ=255)

                                         gCCTATCTCTCAGGCGTCAAAT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAGTCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATCATACTTATTGGCTTAGTACACGCaacaaataatt                                                                                                 >  1:645268/1‑132 (MQ=255)

                                          ccTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                                                                                 >  1:767668/1‑141 (MQ=255)

                                          ccTATCTCTCAGGCGTCAATT‑‑‑GACGAAGATAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                <  2:42778/141‑11 (MQ=255)

                                           cTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGCGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATCGCACCAGTCCTCAGTGGCTGTGCTGTTTCGTATTAGACTTATTGGCTTAGTAAACGCacgaagtaattat                                                                                               >  2:298574/1‑129 (MQ=255)

                                           cTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                               >  1:699709/1‑130 (MQ=255)

                                           cTATCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                               >  1:975657/1‑130 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                                                                              >  1:1023345/1‑141 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGACATTGCGTTTTTTTCTTATTGGTTAAGTAGACGCACTATAAAATAggg                                                                                              >  2:958987/1‑140 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                             >  2:605352/1‑128 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                             >  1:418314/1‑128 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                             >  2:695494/1‑128 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACTTAGTAAACGCaacaaataattaata                                                                                             >  2:89379/1‑128 (MQ=255)

                                             aTCTCTCAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                             >  1:189835/1‑128 (MQ=255)

                                              tctctcAGGCGTCAATT‑‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                            >  2:852191/1‑127 (MQ=255)

                                               ttctgAGGCTTCAAGT‑TAGACGCAGAGAGGAGGGGTA‑GAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGa                                                                                            <  1:441641/140‑1 (MQ=255)

                                                tctcAGGCGTCAATT‑TACACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGCGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAc                                                                                           <  2:709455/141‑1 (MQ=255)

                                                   cAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                                                                        <  2:178970/141‑1 (MQ=255)

                                                    aGGCGTCAATT‑TAGACGCGGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCACTACAAAATAGGTGAAatat                                                                                       >  1:210056/1‑141 (MQ=255)

                                                    aGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATCCAAAAAAGGTGCCatat                                                                                       >  1:76182/1‑141 (MQ=255)

                                                           aaTT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                                                                >  2:925655/1‑141 (MQ=255)

                                                           aaTT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                                                                >  1:608925/1‑141 (MQ=255)

                                                             tt‑tAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCACTAGAAAATAGGTGACATATAGCCGCAcc                                                                              >  1:487394/1‑141 (MQ=255)

                                                             tt‑tAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCCTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                                                                              <  2:836007/141‑1 (MQ=255)

                                                                 aGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAt                                                                           <  1:794324/141‑1 (MQ=255)

                                                                  gatgCAGAGTGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                                                                          <  2:672060/138‑1 (MQ=255)

                                                                     gCAGGGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa                                                                       <  1:345224/141‑1 (MQ=255)

                                                                       agagagGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGCCATATAGCAGCCCCAATAAAAAt                                                                     >  1:132380/1‑141 (MQ=255)

                                                                          gaggagGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATccc                                                                  <  1:699398/141‑1 (MQ=255)

                                                                            ggaggGGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                                                                <  2:292634/141‑1 (MQ=255)

                                                                               ggTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                             <  1:23388/141‑1 (MQ=255)

                                                                               ggTGTA‑TAA‑G‑G‑TGATGCTTACTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                             <  2:308173/141‑1 (MQ=255)

                                                                                  gtA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAc                                                          >  2:735290/1‑141 (MQ=255)

                                                                                       aa‑G‑G‑TGGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCa                                                      >  2:350452/1‑141 (MQ=255)

                                                                                        a‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTGTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaa                                                     >  2:908138/1‑141 (MQ=255)

                                                                                               gatgttgtttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAg                                                 <  1:496544/133‑1 (MQ=255)

                                                                                                   cTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAg                                             >  2:468686/1‑141 (MQ=255)

                                                                                                    ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTATTTTCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATACAAATCCCCTCACTACCGCAAATAGTGAgg                                            >  2:11435/1‑141 (MQ=255)

                                                                                                    ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                            <  1:511082/141‑1 (MQ=255)

                                                                                                    ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                            <  2:188102/141‑1 (MQ=255)

                                                                                                    ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTGTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                            >  1:1007127/1‑141 (MQ=255)

                                                                                                     ttttttCGTTCCCATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                           <  1:175705/139‑1 (MQ=255)

                                                                                                     tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                           >  1:55913/1‑141 (MQ=255)

                                                                                                     tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAGTGCGTTTTTTTGTTATTGGCTAAGTAGCCGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCCCTACCGCAAATAGTGAggg                                           >  1:407725/1‑141 (MQ=255)

                                                                                                      aTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                          >  1:825140/1‑141 (MQ=255)

                                                                                                       ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                         <  2:44583/141‑1 (MQ=255)

                                                                                                          tCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGGGGGGAtgg                                      >  1:396407/1‑139 (MQ=255)

                                                                                                          tCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAAGACAAGATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGAGTg                                      >  2:528525/1‑141 (MQ=255)

                                                                                                                     atAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGTGGTATAAGTa                           >  2:243478/1‑141 (MQ=255)

                                                                                                                       aGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACCTATAGCCGCACCCATAAAATGCCCCGCCATTCCGCAAATAGTGAGGGGATTGGTGTAtaaataaa                         >  2:744662/1‑141 (MQ=255)

                                                                                                                                catcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                >  2:726838/1‑141 (MQ=255)

                                                                                                                                aatcgttgccTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                <  1:392388/132‑1 (MQ=255)

                                                                                                                                 atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCGACTAGTGAAGGGGGTGGTGGAGAGGTAAGTACtgcttat               >  1:325314/1‑135 (MQ=255)

                                                                                                                                      tGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTg          <  2:1050144/141‑1 (MQ=255)

                                                                                                                                       ggCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGATGTAGTAAATACTTATTTTCGTTGt         <  2:1023864/141‑1 (MQ=255)

GACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATT‑TAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATT  >  CP000730/1929779‑1930056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`