Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,725,352 A→T 6.2% intergenic (‑58/+276) USA300HOU_2578 ← / ← USA300HOU_2579 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,725,3520AT 6.2% 223.9 / 3.8 80intergenic (‑58/+276)USA300HOU_2578/USA300HOU_2579hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (45/29);  new base T (4/2);  total (49/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATACCGAAATCTTTTAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGTT  >  CP000730/2725214‑2725487
                                                                                                                                          |                                                                                                                                       
tcattcataaTCTTTTAAGGTAATGGCCATACCAATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATAGTAACACGCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATAGtaatatt                                                                                                                                       >  2:701519/9‑139 (MQ=255)
 ataCCGAAATCTTTTAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAAtt                                                                                                                                      <  2:331228/141‑1 (MQ=255)
  taCCGAAATCTTTTAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTa                                                                                                                                     >  1:788019/1‑141 (MQ=255)
    cttaaagcgtttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACg                                                                                                                                   <  2:789170/132‑1 (MQ=255)
    ccGAAATCTTTTAAGGGAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACg                                                                                                                                   <  1:750605/141‑1 (MQ=255)
     cGAAATCTTTTAAGGAAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGa                                                                                                                                  <  1:756145/141‑1 (MQ=255)
            ttttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATaa                                                                                                                           >  2:244686/1‑141 (MQ=255)
            ttttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATaa                                                                                                                           <  1:941155/141‑1 (MQ=255)
            ttttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATaa                                                                                                                           >  1:628397/1‑141 (MQ=255)
            ttttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATaa                                                                                                                           >  2:1025715/1‑141 (MQ=255)
            ttttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATaa                                                                                                                           >  1:913919/1‑141 (MQ=255)
              ttAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAg                                                                                                                         >  1:260255/1‑141 (MQ=255)
               tAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTATTTTTGATTATAAATCATAATAATTACGATTTATAAAGt                                                                                                                        >  2:18361/1‑141 (MQ=255)
                 aGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGtat                                                                                                                      <  1:732901/141‑1 (MQ=255)
                         gCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATTATGACGATTTATATAGTATAGACCTAt                                                                                                              >  2:169923/1‑141 (MQ=255)
                         gCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTAt                                                                                                              <  2:1022744/141‑1 (MQ=255)
                          ccATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATc                                                                                                             <  2:214931/141‑1 (MQ=255)
                          ccAGACCTAGACCTGCAGCACCGGCACCGATAACAGCCATTTTCTTTGGTCGCTGACTAACATTACTTAAACGCTAGCTTTAAATCTATTGTTTTTGAATTTTATTCGTAAGAATTACGATGTATAAAGTATAGACCTATc                                                                                                             <  2:209872/141‑1 (MQ=255)
                           cATAGCTATACCAGCAGCACCGGCACCGATAATAGCCACTTTATGAGGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGAATATAACTCGTAATAATTACGATTTATAGAGTATAGACCTATcc                                                                                                            >  1:748502/1‑141 (MQ=255)
                             tacctatacctGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCtt                                                                                                          >  2:578073/1‑141 (MQ=255)
                              acctatacctGCAGCACCGGTACCGATAATAGCCACTTTACGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTg                                                                                                         <  2:542013/141‑1 (MQ=255)
                               cctttaccgTCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTga                                                                                                        <  2:713540/141‑1 (MQ=255)
                               cctatacctGCAGCACCCGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTga                                                                                                        <  2:713281/141‑1 (MQ=255)
                                 tatacctGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTgaga                                                                                                      <  2:1038696/141‑1 (MQ=255)
                                    accgGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCCCATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAAt                                                                                                   <  1:552044/141‑1 (MQ=255)
                                          agcaCCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACCCTCACTCAATGATCTATTTGAAGTTACTGTTTCTGATTATAAATCGTAACACTTACGGTTGCAAAAGTATGGCCCTATCCTTTAGAAACTTCAat                                                                                             >  2:51377/1‑141 (MQ=255)
                                             accggcaccgATAATAGCCACTTTATGATGTTGCATATTAACGCTCCCTCAATGATCTATTTCACGTTATTGTTTTTGCTTATAAATCGTAATAATTACGAATTGTAAAGTATAAACGTATCCTTTAGAAATTTCAATAtt                                                                                          >  2:79469/1‑141 (MQ=255)
                                             accggcaccgATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATGGACCTATCCTTGAGAAATTTCAATAtt                                                                                          >  2:101029/1‑141 (MQ=255)
                                             accggcaccgATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATACTTGGGaacattctagatt                                                                                          >  2:951912/1‑130 (MQ=255)
                                               cggcacctATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTaa                                                                                        >  1:667693/1‑141 (MQ=255)
                                                ggcaccgATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTTTTTTTTATTCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGCGAACTTTCTATGTTtaa                                                                                       >  2:558656/1‑141 (MQ=255)
                                                    ccgATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCCATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGaa                                                                                   >  2:460138/1‑141 (MQ=255)
                                                          ataGCCACTTTATGATGTTGCATATTAACACTCCCTCAAAGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACtt                                                                             >  1:853664/1‑141 (MQ=255)
                                                           aaGCCACCTTCTGATGTTGCATATTAACACTCCCTCCATGATCTATCTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTc                                                                            <  1:131849/140‑1 (MQ=255)
                                                               cACTTTACGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGtt                                                                        >  2:130173/1‑141 (MQ=255)
                                                                 cTTTATGATGTTGCATATTAACACTCCCTCCATGATCTATTTCAAGGTATTGTTTTTGATTATAAATCGTAATAATTACGATTTCTAAAGTATAGACCTATCCTTGAGAAATATCAATATTAAAAGAAAAACTTCCGTTAt                                                                      >  2:373357/1‑141 (MQ=255)
                                                                  tttATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTAtt                                                                     >  2:313259/1‑141 (MQ=255)
                                                                   ttATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGTTGAATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTg                                                                    >  1:772633/1‑141 (MQ=255)
                                                                   ttATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCAGTTATTg                                                                    >  2:528596/1‑141 (MQ=255)
                                                                    tATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGt                                                                   >  1:622133/1‑141 (MQ=255)
                                                                    tATGATGTTGCATATTAACACTCCCCCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGt                                                                   <  1:319505/141‑1 (MQ=255)
                                                                      tgatgTTGCATATTAACACTCCCTCAATGATCTATTTCACGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCACTATTGAAAGAAAGACTTCCGTTATTGTag                                                                 >  1:87570/1‑141 (MQ=255)
                                                                       gatgTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTaga                                                                <  1:467178/141‑1 (MQ=255)
                                                                              cATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGtttatcactga                                                         <  2:651009/141‑11 (MQ=255)
                                                                               atatTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTGTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCAt                                                        <  1:163294/141‑1 (MQ=255)
                                                                                     acacTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGCGAATTCATCTACAt                                                  >  2:360028/1‑141 (MQ=255)
                                                                                      cacTCCCTCAATGAACTATTTCAAGATATTGTTTCTGATTATCAATCGTAATACTAACGATTTGTCAAGTATACCCCTATCCTTGAGAAATTTCACACTTAAAAGAAAAACTTCCGTTATTGTAGAGAATTTATCTACAtt                                                 >  2:54716/1‑141 (MQ=255)
                                                                                         tCCCTCAATGATTTATTTCGACTTATCGTTTTTGATTAGAAATCGTAATAATGACTATGTATAAAGGATGGTCCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTGCCGTTATTGTAGATAATTCATCTACATTTCg                                              <  2:3429/141‑1 (MQ=255)
                                                                                         ctccTCAATTATCTATTTTAAGTTATTGTTTTTGATTATAAATCGTAATAATTAAGCTTCATAACGTATTGACCTATCCTTGACGAATTTCAATATCAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCg                                              <  1:470415/139‑1 (MQ=255)
                                                                                            cTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTCCCGTTATTGTAGAGAATTCATCTACATTTCGGaa                                           >  1:582866/1‑141 (MQ=255)
                                                                                            cTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTAAGATTTATAAAGTATAGACCTATCCTTGAGGAATTTCAATATAAAAAAAAAAACTTCCGTTATTTTAGAGAATTCATATCCATTCGGAAg                                          >  2:820683/1‑141 (MQ=255)
                                                                                             tCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTTTAGAGAATTCATCTACATTTCGGAAg                                          >  1:66890/1‑141 (MQ=255)
                                                                                                aTGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACTTATCCTTGATAAATTTTAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAGGtgg                                       >  1:470458/1‑139 (MQ=255)
                                                                                                 ttaTCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAAGATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTgg                                      <  2:170754/139‑1 (MQ=255)
                                                                                                 tGATCTATTTCAAGGTATTGTTTTTGATTCTAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTAATTATTAAAAGAAAAACTTCCGTTATTAGGGAGGTGTCATCTACATTTCGGAAGTTgg                                      >  2:479452/1‑141 (MQ=255)
                                                                                                  gATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTGGGAATTTGGt                                     >  1:110674/1‑141 (MQ=255)
                                                                                                  gATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGt                                     <  2:853664/141‑1 (MQ=255)
                                                                                                     cTATTTCAAGTTATTGTTTTAGATTATACATCGTAATAATTACGATTTATAAAGCATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTAtg                                  >  1:686271/1‑141 (MQ=255)
                                                                                                      tATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTCGGTAtgt                                 >  2:230839/1‑141 (MQ=255)
                                                                                                        tttCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATgtgt                               <  1:473206/141‑1 (MQ=255)
                                                                                                               tttttGTGTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCtttttt                        <  2:789711/138‑1 (MQ=255)
                                                                                                               ttATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCtttttt                        <  2:21558/141‑1 (MQ=255)
                                                                                                               ttATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCtttttt                        >  2:673352/1‑141 (MQ=255)
                                                                                                                tacttgttttGATTATAAATCGGAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCttttttt                       <  1:76983/135‑1 (MQ=255)
                                                                                                                tATTGTTTTTGATTATAAATCGTATTAATTACGATTTATAAAGTCTAGACCTATCCTTGAGCAATTTCAATATTACAAGAAAAACTTCCGTTATTGTAGCGAATTCATCTCCATTTCGGACGTTGGTATGTGTCttttttt                       >  1:515662/1‑141 (MQ=255)
                                                                                                                 aTTGTTTTTGATTATAAATCGTAATAATTAAGATTTATCAAGTCTAGCAATATATTTGAGAAAATGTAATAGCAAAAGATAACCATCTATTGTGATAACAGACTCATCTGCAGTGCGTAGGTTGGTATTTGTCtttttttt                      >  2:509635/1‑140 (MQ=255)
                                                                                                                    gTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCttttttttttt                   >  2:777089/1‑137 (MQ=255)
                                                                                                                     tttttGATTATGAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTACAAt                  >  2:650461/1‑141 (MQ=255)
                                                                                                                     tttttGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCAGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGGCTTTTTTTCAAAt                  >  2:24094/1‑141 (MQ=255)
                                                                                                                     tttttGATCATAAATCGTAACTATATCGATTTATAAAGTATAGCCCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAAt                  <  1:485116/141‑1 (MQ=255)
                                                                                                                             tataAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGgct          >  1:49628/1‑141 (MQ=255)
                                                                                                                              ataAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATGTTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTAGGTGTCTTTTTTTAAAATTTACGgctg         >  2:585373/1‑141 (MQ=255)
                                                                                                                               taAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTACACTTGTAGAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGgctgc        <  1:244686/141‑1 (MQ=255)
                                                                                                                                 aaTCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTGGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTa      >  1:827149/1‑141 (MQ=255)
                                                                                                                                  aTCGTAATTATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAGAATTTAAGGCTGGTAc     >  2:1058363/1‑141 (MQ=255)
                                                                                                                                  aTCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTAc     >  1:149999/1‑141 (MQ=255)
                                                                                                                                   tCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTagg    >  1:236910/1‑139 (MQ=255)
                                                                                                                                    cttaataaTTACGAAATATAAAGGATAAACCTAGCCTTGAGCACTTTCCATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGt   <  1:873280/139‑1 (MQ=255)
                                                                                                                                    cGTAATTATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGt   >  1:622264/1‑141 (MQ=255)
                                                                                                                                    cGTAATTATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGt   >  1:364980/1‑141 (MQ=255)
                                                                                                                                    cGTAATAAATACGATTTATGAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGt   <  1:678128/141‑1 (MQ=255)
                                                                                                                                     gTAATTATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGtt  <  2:784526/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
TATACCGAAATCTTTTAAGGTAATGGCCATACCTATACCTGCAGCACCGGCACCGATAATAGCCACTTTATGATGTTGCATATTAACACTCCCTCAATGATCTATTTCAAGTTATTGTTTTTGATTATAAATCGTAATAATTACGATTTATAAAGTATAGACCTATCCTTGAGAAATTTCAATATTAAAAGAAAAACTTCCGTTATTGTAGAGAATTCATCTACATTTCGGAAGTTGGTATGTGTCTTTTTTTAAAATTTAAGGCTGCTACGTT  >  CP000730/2725214‑2725487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: