Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 531,823 A→G 13.4% intergenic (+1394/‑4331) USA300HOU_0494 → / → rplY hypothetical protein/ribosomal protein L25

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730531,8230AG13.4% 61.8 / 5.3 30intergenic (+1394/‑4331)USA300HOU_0494/rplYhypothetical protein/ribosomal protein L25
Reads supporting (aligned to +/- strand):  ref base A (13/13);  new base G (2/2);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-02

GAAAAAATTTAAACAGGCGAAGTCTTCAATAAGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGTTGGT  >  CP000730/531690‑531957
                                                                                                                                     |                                                                                                                                      
gAAAAAATTTAAACAGGCGAAGTCTTCAATAAGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTaa                                                                                                                                 >  1:220830/1‑141 (MQ=255)
                             aaaGTGATGATTTCCAAATTAGAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAgg                                                                                                    <  2:249573/140‑1 (MQ=255)
                             aaaGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAATTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAgg                                                                                                    <  2:249654/140‑1 (MQ=255)
                                gTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCa                                                                                                 >  2:11677/1‑141 (MQ=255)
                                atGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCa                                                                                                 <  1:256135/140‑1 (MQ=255)
                                 tGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCAt                                                                                                >  1:308295/1‑141 (MQ=255)
                                            aaaTTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTag                                                                                     >  2:300072/1‑141 (MQ=255)
                                                  taAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAACCCAATGATGAGTAAAGAAGCGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAtaga                                                                               <  2:289051/141‑1 (MQ=255)
                                                  taAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAgaga                                                                               >  1:54131/1‑141 (MQ=255)
                                                    aaaaaTACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGagaaa                                                                             >  1:272687/1‑141 (MQ=255)
                                                    aaaaaTACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGagaaa                                                                             >  1:272717/1‑141 (MQ=255)
                                                    aaaaaTACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGATGTCATTACTCTATTAGAAGagaaa                                                                             <  1:251639/141‑1 (MQ=255)
                                                                      gaaaagTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTggg                                                           >  2:309044/1‑140 (MQ=255)
                                                                          agTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGat                                                       <  2:272687/141‑1 (MQ=255)
                                                                          agTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGat                                                       <  2:272717/141‑1 (MQ=255)
                                                                                          tactacTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGa                                       >  1:268133/1‑141 (MQ=255)
                                                                                              actGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTg                                   >  1:259113/1‑141 (MQ=255)
                                                                                                   tgGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGGAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTTCCTGGCGGATATTTATTTTTATCAGATTTggtagg                              >  2:24551/1‑141 (MQ=255)
                                                                                                    ggCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTggtaggt                             <  1:19716/141‑1 (MQ=255)
                                                                                                     gcgcAAGTGGTGGTGTTACGTATAAACCAATGATCAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTggtaggta                            <  2:328712/141‑1 (MQ=255)
                                                                                                       gcAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGACCGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAAt                          <  1:11677/141‑1 (MQ=255)
                                                                                                        cAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATc                         <  1:281975/141‑1 (MQ=255)
                                                                                                          aggggtggtgTTACGTAAAAACTAAAGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCa                       <  1:281769/138‑1 (MQ=255)
                                                                                                              gtggtgTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACATGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGc                   >  1:280098/1‑141 (MQ=255)
                                                                                                                 gtgtTACGTAGAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGctac                <  2:269976/141‑1 (MQ=255)
                                                                                                                  tgtTACGTAAAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGctact               <  1:114443/141‑1 (MQ=255)
                                                                                                                    ttACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATTCATCGCTACTaa             <  1:243763/141‑1 (MQ=255)
                                                                                                                       cttagaaaCCAAGGATGAGTAAAAAAGAGGCGACTGAAGTTGTTAATGAGTTCATTACTCAATTAAAAGAGAAAGAACGTTTGTTACCTAGCGCATATTTATGTTTATCTTATTTGGTAGGTCATCCATCGCGACTAAACa          >  2:89178/6‑141 (MQ=255)
                                                                                                                           taAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGt      >  2:94651/1‑141 (MQ=255)
                                                                                                                               ccAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGTTGGt  >  1:282048/1‑141 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GAAAAAATTTAAACAGGCGAAGTCTTCAATAAGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGTTGGT  >  CP000730/531690‑531957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: