| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 531,823 | A→G | 13.4% | intergenic (+1394/‑4331) | USA300HOU_0494 → / → rplY | hypothetical protein/ribosomal protein L25 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 531,823 | 0 | A | G | 13.4% | 61.8 / 5.3 | 30 | intergenic (+1394/‑4331) | USA300HOU_0494/rplY | hypothetical protein/ribosomal protein L25 |
| Reads supporting (aligned to +/- strand): ref base A (13/13); new base G (2/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-02 | |||||||||||
GAAAAAATTTAAACAGGCGAAGTCTTCAATAAGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGTTGGT > CP000730/531690‑531957 | gAAAAAATTTAAACAGGCGAAGTCTTCAATAAGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTaa > 1:220830/1‑141 (MQ=255) aaaGTGATGATTTCCAAATTAGAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAgg < 2:249573/140‑1 (MQ=255) aaaGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAATTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAgg < 2:249654/140‑1 (MQ=255) gTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCa > 2:11677/1‑141 (MQ=255) atGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCa < 1:256135/140‑1 (MQ=255) tGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCAt > 1:308295/1‑141 (MQ=255) aaaTTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTag > 2:300072/1‑141 (MQ=255) taAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAACCCAATGATGAGTAAAGAAGCGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAtaga < 2:289051/141‑1 (MQ=255) taAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAgaga > 1:54131/1‑141 (MQ=255) aaaaaTACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGagaaa > 1:272687/1‑141 (MQ=255) aaaaaTACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGagaaa > 1:272717/1‑141 (MQ=255) aaaaaTACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGATGTCATTACTCTATTAGAAGagaaa < 1:251639/141‑1 (MQ=255) gaaaagTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTggg > 2:309044/1‑140 (MQ=255) agTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGat < 2:272687/141‑1 (MQ=255) agTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGGTGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGat < 2:272717/141‑1 (MQ=255) tactacTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGa > 1:268133/1‑141 (MQ=255) actGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTg > 1:259113/1‑141 (MQ=255) tgGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGGAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTTCCTGGCGGATATTTATTTTTATCAGATTTggtagg > 2:24551/1‑141 (MQ=255) ggCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTggtaggt < 1:19716/141‑1 (MQ=255) gcgcAAGTGGTGGTGTTACGTATAAACCAATGATCAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTggtaggta < 2:328712/141‑1 (MQ=255) gcAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGACCGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAAt < 1:11677/141‑1 (MQ=255) cAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATc < 1:281975/141‑1 (MQ=255) aggggtggtgTTACGTAAAAACTAAAGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCa < 1:281769/138‑1 (MQ=255) gtggtgTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACATGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGc > 1:280098/1‑141 (MQ=255) gtgtTACGTAGAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGctac < 2:269976/141‑1 (MQ=255) tgtTACGTAAAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGctact < 1:114443/141‑1 (MQ=255) ttACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATTCATCGCTACTaa < 1:243763/141‑1 (MQ=255) cttagaaaCCAAGGATGAGTAAAAAAGAGGCGACTGAAGTTGTTAATGAGTTCATTACTCAATTAAAAGAGAAAGAACGTTTGTTACCTAGCGCATATTTATGTTTATCTTATTTGGTAGGTCATCCATCGCGACTAAACa > 2:89178/6‑141 (MQ=255) taAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGt > 2:94651/1‑141 (MQ=255) ccAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGTTGGt > 1:282048/1‑141 (MQ=255) | GAAAAAATTTAAACAGGCGAAGTCTTCAATAAGTGAAGATGTCCAAATTATAAAAAATACATTCCAAAAAGAAAAGTTAGGTACAGTAATTACTACTGCTGGCGCAAGTGGTGGTGTTACGTATAAACCAATGATGAGTAAAGAAGAGGCGACTGAAGTTGTTAATGAGGTCATTACTCTATTAGAAGAGAAAGAACGTTTGTTACCTGGCGGATATTTATTTTTATCAGATTTGGTAGGTAATCCATCGCTACTAAACAAAGTTGGT > CP000730/531690‑531957 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |