Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,481,355 G→T 100% A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,481,3550GT100.0% 85.9 / NA 30A6890E (GCA→GAA) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/18);  total (12/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTT  >  CP000730/1481229‑1481483
                                                                                                                              |                                                                                                                                
ttCAGTTCCTGTGCTGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTTATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTCAGTTAGCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCa                                                                                                                    <  1:150648/141‑1 (MQ=255)
ttCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCa                                                                                                                    >  2:267232/1‑141 (MQ=255)
ttCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCa                                                                                                                    <  1:50594/141‑1 (MQ=255)
   aGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCt                                                                                                                 <  1:171750/141‑1 (MQ=255)
           aGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTGCttt                                                                                                         >  2:16186/1‑141 (MQ=255)
              ggTTGGAGTGTCTTGCGTTGCTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCt                                                                                                      <  2:16212/141‑1 (MQ=255)
                       ggCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAtt                                                                                             <  1:53066/141‑1 (MQ=255)
                         cTTGCGTTAGTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCa                                                                                           >  1:252908/1‑141 (MQ=255)
                              gTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCt                                                                                      <  1:221636/141‑1 (MQ=255)
                                  cTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGtt                                                                                  >  2:203865/1‑141 (MQ=255)
                                         tACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgt                                                                           >  1:35766/1‑141 (MQ=255)
                                         tACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgt                                                                           >  2:234791/1‑141 (MQ=255)
                                                 gggcTTGATTGATGTTTCCAGTAAACGTACCTTTTTGTGCATTGTTTAAGTTTTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAg                                                                   <  2:139924/139‑1 (MQ=255)
                                                 gcgcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAg                                                                   >  2:315504/1‑141 (MQ=255)
                                                       gattgatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGaaa                                                             <  1:298330/141‑1 (MQ=255)
                                                              gTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTCAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGtgctgc                                                      >  1:121993/1‑141 (MQ=255)
                                                                  cctgtacctgCACCTTTTTGTGCTTTGTTTAAGCTATCTAACGTATATTGTGTTTGGTTTTCGTTGTTTTTAGCATCTGTTACTTTTCTATTCCCATGCAACGCTTGTTTCGGAGTTGTTACAGTTGAAAGTGCTGCTTCa                                                  <  1:233671/132‑1 (MQ=255)
                                                                  ccAGTAACGGCACCGTGTTGTGCATTGTTTAAGTTACCTAACGTAGATAATGTTTGGTTTTCAGTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCa                                                  <  1:206723/141‑1 (MQ=255)
                                                                        aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTtgg                                            >  1:6917/1‑140 (MQ=255)
                                                                              ccTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTgg                                      <  1:196572/141‑1 (MQ=255)
                                                                                         aTTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGt                           <  2:251984/141‑1 (MQ=255)
                                                                                          ttGTTTAAGTTCTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTa                          <  2:282196/141‑1 (MQ=255)
                                                                                             tttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATATGTTACTTGTCTGTCTCCTTGCAATGCTTGTTTCGTAGTTGTTACAGTGGAAATTGCTGCTTCAAGTTTTGTTTGGTTCGCGTTTTTAccg                       >  2:189042/1‑140 (MQ=255)
                                                                                               tAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAtt                     <  1:66203/141‑1 (MQ=255)
                                                                                                   ttATCTAACGTAGATATTGTTTGGTTTTCATTCTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTTTTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCt                 <  2:67230/141‑1 (MQ=255)
                                                                                                        tacgttAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCACCTTGTGATTGGTTCGCATTTGTACCATTTGCTTGAtt            >  1:119070/6‑141 (MQ=255)
                                                                                                          aCGTAGATAATGTTTGGTTTTCATTGTTTTTTGCATCTGTTTCTTTTCTATTGCCATTCAACGCTTTTTTCGTAGTTGTTACAGTTGAAAGTGCTTGTTCAATTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa          <  2:61476/141‑1 (MQ=255)
                                                                                                          aCGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa          >  1:171293/1‑141 (MQ=255)
                                                                                                            gtgggtAATGTTGTGTTTTCAGTGTTTGGAGCAGCTGTTACTTTTGTATCAGCATTGAACGCTTGTTTCGTAGTTGTGACAGTTGAAAGTGCTGCTTCAACGTGTGGGTGGTTCGCAGTGTTACCGTTTGGGGGATtaata        <  2:333961/136‑1 (MQ=255)
                                                                                                                  aaTGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAttt  <  1:300712/141‑1 (MQ=255)
                                                                                                                              |                                                                                                                                
TTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTT  >  CP000730/1481229‑1481483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑