Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,775,439 C→T 62.6% G362D (GGT→GAT)  clpX ← S14 family endopeptidase ClpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,775,4390CT62.6% 22.0 / 16.0 26G362D (GGT→GAT) clpXS14 family endopeptidase ClpX
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base T (10/6);  total (13/13)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGTCGTATAGTTCTGGTTCAGTTTCTTCATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAGGTT  >  CP000730/1775300‑1775559
                                                                                                                                           |                                                                                                                        
tGCGTCGTATAGTTCTGGTTCAGTTTCTTCATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCAcc                                                                                                                         >  1:238734/1‑141 (MQ=255)
   gtcgtATAGTTCTGGTTCAGTTTCTTCATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGt                                                                                                                      <  1:5229/141‑1 (MQ=255)
    tcgtATAGTTCTGGTTCAGTTTCTTCATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTTCCGCTTCTTCTATGATTTGACGTATACCACGGGGACCTGtt                                                                                                                     >  1:260102/1‑141 (MQ=255)
                    cAGTTTCTTCATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCtt                                                                                                     >  1:315101/1‑141 (MQ=255)
                          ttttcttAATTGTTTGTCCTGTAATAACTACCTTCGTTACATTTTCGTTCGCAGGCACATCAAACATAATATCGATTAACGATTCTTTTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCAc                                                                                               <  2:110225/136‑1 (MQ=255)
                           ttcATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACt                                                                                              >  1:3379/1‑141 (MQ=255)
                                catttgtttgtGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTg                                                                                         <  2:346133/138‑1 (MQ=255)
                                        tgtgCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCGCTAATTGCTGATaaa                                                                                 >  2:110546/1‑141 (MQ=255)
                                          ggCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGc                                                                               <  1:181821/140‑1 (MQ=255)
                                                aataaCTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCttcttc                                                                         <  1:314130/141‑1 (MQ=255)
                                                      tACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTAATAAAGCTTCGTCAGTGaa                                                                   >  1:174779/1‑141 (MQ=255)
                                                          ttCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAActct                                                               >  1:240520/1‑141 (MQ=255)
                                                                   ttttCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGGTTGAACGTAAACCACGCGCATCTGTTTTTCATATTTTTTTGTTTTCACTAATTGCTGATAAAGGTTCTTCAGTGAACTCTTAATCtaca                                                      >  1:148957/1‑141 (MQ=255)
                                                                         ttAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACTACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGATTCTTCAGTGAACTCTAAATCCACATCATCt                                                >  1:207515/1‑141 (MQ=255)
                                                                            gAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAAt                                             >  1:277819/1‑141 (MQ=255)
                                                                               ggCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTcc                                          <  2:315101/141‑1 (MQ=255)
                                                                               ggCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTcc                                          <  2:238734/141‑1 (MQ=255)
                                                                                 cacaTCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAg                                        >  2:125763/1‑141 (MQ=255)
                                                                                         aCATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAg                                >  1:47701/1‑141 (MQ=255)
                                                                                           ataataTCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGta                              <  1:90637/141‑1 (MQ=255)
                                                                                                        aCGATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACaa                 <  1:203325/141‑1 (MQ=255)
                                                                                                          gATTCTTCTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGt               <  2:311578/141‑1 (MQ=255)
                                                                                                            ttcttcTATGATTGAACGTAAACCACGCGCATCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCAACATCATCTTATTCCAGCAGTTTAGTATATTGTTTCACAAGTGc             >  1:93735/1‑141 (MQ=255)
                                                                                                             tcttctATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTATTCAGTTAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCa            >  1:323334/1‑141 (MQ=255)
                                                                                                                 ctATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTCCGAGTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCAtttt        <  1:233371/141‑1 (MQ=255)
                                                                                                                  tATGATTGAACGTACACCACGCTCACCTTTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATTAAGCTTCTTCAGTTAGCTCTAAATCCTCATCATCTCATTCCAGCATTTTAGTATATTGTTTCACAAGTGCAttttt       <  2:300605/141‑1 (MQ=255)
                                                                                                                       tagaccctAAACCACGCGCACCTGTTTTTCTTTTAATTGCTTTTTCACTAATTGCTGATTAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAGGtt  <  2:329644/134‑1 (MQ=255)
                                                                                                                                           |                                                                                                                        
TGCGTCGTATAGTTCTGGTTCAGTTTCTTCATTAATTGTTTGTGCTGTAATAACTACCTTCGTTACATTTTCGTTAGAAGGCACATCAAACATAATATCGATTAACGATTCTTCTATGATTGAACGTAAACCACGCGCACCTGTTTTTCTTTCAATTGCTTTTTCACTAATTGCTGATAAAGCTTCTTCAGTGAACTCTAAATCCACATCATCTAATTCCAGCATTTTAGTATATTGTTTCACAAGTGCATTTTTAGGTT  >  CP000730/1775300‑1775559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑