Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,797,772 G→C 100% R7G (CGC→GGC)  USA300HOU_1679 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,797,7720GC100.0% 99.1 / NA 30R7G (CGC→GGC) USA300HOU_1679sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/14);  total (16/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAAT  >  CP000730/1797644‑1797906
                                                                                                                                |                                                                                                                                      
tGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACtt                                                                                                                            >  1:24103/1‑141 (MQ=255)
 gATCTTGCATCTGTTGCTCGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATGCTGCTTGTAAATAACATTCGGCCTTGGTGGAGCTtc                                                                                                                           >  1:74601/1‑141 (MQ=255)
   tCTTGCATCTGTTTCTATACACTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAAAAACATTAAGCCGTGGTGAAACTtcat                                                                                                                         <  1:174541/141‑1 (MQ=255)
       gCATCTTTTGCTAGAGCTTGTTAGTGTTTTTCTGATAAATCATATGCGGTGGAAGCGTGATTAAGAATGCCTAATGTAACAAAACTAGTCGTTGATATATTTCTTATCAATAACATTAAGGCGTGGTGAACTTTCAGCAtt                                                                                                                     >  2:134009/1‑141 (MQ=255)
         aTCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGt                                                                                                                   <  2:64140/141‑1 (MQ=255)
             gTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCtt                                                                                                               <  2:327427/141‑1 (MQ=255)
                      gCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCttt                                                                                                      <  1:271622/141‑1 (MQ=255)
                       cTTGTTCGTGTTTTTCTGATAAAACATCTGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTc                                                                                                     <  2:70979/141‑1 (MQ=255)
                       cTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTc                                                                                                     >  1:174726/1‑141 (MQ=255)
                                               cATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCAACGcac                                                                             >  1:5701/1‑141 (MQ=255)
                                                 tatGTGATGGAAGCCTAAATAATAAGGCTCAATGTAACAAAACTGATAATTAATATACTTCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAg                                                                           <  2:42360/141‑1 (MQ=255)
                                                  atGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGt                                                                          >  1:75495/1‑141 (MQ=255)
                                                     tgATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGtttt                                                                       >  1:13672/1‑141 (MQ=255)
                                                             gCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGtt                                                               >  1:102325/1‑141 (MQ=255)
                                                              cGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTtg                                                              <  1:7322/141‑1 (MQ=255)
                                                                tGAAAAATAATGCCTAATGTAACAAAACTGCTAATTAATATAGTGCTTAACAAGAACAATAAGCCGAGGTGAAACTTCATCATTGTTCTTTCGGTCTTTCCAATTTATAGCCTAAGGCACGCCCAGTTTTAATAAGTtgtt                                                            >  2:209526/1‑140 (MQ=255)
                                                                         atGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCAGAGTTTTAATAAGTTGTGGCTTCTTAg                                                   <  2:214440/141‑1 (MQ=255)
                                                                         atGCCAAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCAGAGTTTTAATAAGTTGTGGCTTCTTAg                                                   <  2:214493/141‑1 (MQ=255)
                                                                                         aaCTAATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCCATTTATAGCCTAAGCCACGCACACTTTTAATAAGTTGTGGCTTCTTAGGTTTTTCTTCTAAttt                                   >  1:243978/1‑141 (MQ=255)
                                                                                                aattaatATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCtt                            >  1:217531/1‑141 (MQ=255)
                                                                                                  ttaatATACTGCTTATCAATAACATGAAGCCGTGGGGACACTTCATCATTGTTCTTTAGGTCTTTCCAATTGATAGCCTCAGCCACGCACAGTTTTAATAAGTTGTGGCTTCGTATGATTATCTTCTAATTTATCTCTCaa                          <  2:151882/141‑1 (MQ=255)
                                                                                                              ttATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATg              <  2:5701/141‑1 (MQ=255)
                                                                                                              ttATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATg              <  1:156416/141‑1 (MQ=255)
                                                                                                              ttATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATg              <  2:325033/141‑1 (MQ=255)
                                                                                                                  cAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACa          >  1:71758/1‑141 (MQ=255)
                                                                                                                    aTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTACTTTATCTCTTAAATGACTGATATGTACATc        >  2:108054/1‑141 (MQ=255)
                                                                                                                    aTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGGTATGTACATc        >  1:30409/1‑141 (MQ=255)
                                                                                                                    aTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATc        >  2:108050/1‑141 (MQ=255)
                                                                                                                       aCATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATcaac     <  2:174726/141‑1 (MQ=255)
                                                                                                                          ttAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAAt  >  2:59557/1‑141 (MQ=255)
                                                                                                                                |                                                                                                                                      
TGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCAACAAT  >  CP000730/1797644‑1797906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: