Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,028,542 G→C 32.7% L35V (CTC→GTC)  USA300HOU_1882 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,028,5420GC32.7% 18.1 / 21.7 26L35V (CTC→GTC) USA300HOU_1882hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (7/9);  new base C (4/4);  total (12/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

TGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACA  >  CP000730/2028407‑2028668
                                                                                                                                       |                                                                                                                              
tgatgatTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTaaaa                                                                                                                           <  2:315692/141‑1 (MQ=255)
    gatTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGcc                                                                                                                       <  1:54023/141‑1 (MQ=255)
             cTTATTAGACAATAATGTTAATGCAATTAATAGCGCGCCTCTCCAAACCGCCAAAAGGCCACGTATTTTATGAAAATAAATATATCCTACATAAACTAATACACATCCCAATAATAGAACTAGTAAAAAGCCAATTTTTTc                                                                                                              <  1:270765/141‑1 (MQ=255)
                      cAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAatat                                                                                                     >  1:273985/1‑141 (MQ=255)
                       aataatGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAAAACGATGAAAAAGCCAATTTTTTCAAAAAatatt                                                                                                    >  1:24875/1‑140 (MQ=255)
                                  tGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAAtt                                                                                         >  2:116206/1‑141 (MQ=255)
                                    cAATTAATCGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTgg                                                                                       <  2:124427/141‑1 (MQ=255)
                                      aTTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCa                                                                                     >  2:306362/1‑141 (MQ=255)
                                         aaTAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCaata                                                                                  <  1:201302/141‑1 (MQ=255)
                                             gctctccTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAAAAAAAAAAAAAAATATGTAGTAAAAATTGGCAATAATCa                                                                              <  2:346074/136‑1 (MQ=255)
                                                            aCGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGTAATAAACCTTAATGCGGTaaaaa                                                               >  1:50250/1‑141 (MQ=255)
                                                               ccAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAAAATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAAAGa                                                            >  2:50449/1‑141 (MQ=255)
                                                                   aaGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAGAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCaa                                                        <  2:273985/141‑1 (MQ=255)
                                                                                 tATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGTTAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGata                                          <  1:150636/141‑1 (MQ=255)
                                                                                         tAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTtg                                  <  1:193916/141‑1 (MQ=255)
                                                                                               aatCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTc                            <  2:33968/140‑1 (MQ=255)
                                                                                                  ccTACATAAACTAATACACATGCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCTATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATg                         >  1:176634/1‑141 (MQ=255)
                                                                                                      cATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTc                     <  1:112880/141‑1 (MQ=255)
                                                                                                       aTAAACTAATACCGTACCCAAAAATAGAACGATTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTcg                    <  1:331055/141‑1 (MQ=255)
                                                                                                         aaaCTAATACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTcgcc                  >  1:223740/1‑141 (MQ=255)
                                                                                                               aTACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTCATATATGAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCttt            >  1:250747/1‑141 (MQ=255)
                                                                                                                   acaTCCCAATAATAGAACAAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTAt        >  1:22601/1‑141 (MQ=255)
                                                                                                                     aacccAATAATAGAACGACTAAAAATCCATTTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATTATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGc      <  1:303505/139‑1 (MQ=255)
                                                                                                                      tCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGcc     >  2:50725/1‑141 (MQ=255)
                                                                                                                        ccAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcac   >  2:216798/1‑141 (MQ=255)
                                                                                                                         cAATAATAGAACTAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATTAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca  <  2:295983/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                              
TGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACA  >  CP000730/2028407‑2028668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: